Abstract:
BACKGROUND:A question of epidemiological relevance in Chagas disease studies is to understand Trypanosoma cruzi transmission cycles and trace the origins of (re)emerging cases in areas under vector or disease surveillance. Conventional parasitological methods lack sensitivity whereas molecular approaches can fill in this gap, provided that an adequate sample can be collected and processed and a nucleic acid amplification method can be developed and standardized. We developed a duplex qPCR assay for accurate detection and quantification of T. cruzi satellite DNA (satDNA) sequence in samples from domestic and sylvatic mammalian reservoirs. The method incorporates amplification of the gene encoding for the interphotoreceptor retinoid-binding protein (IRBP), highly conserved among mammalian species, as endogenous internal amplification control (eIAC), allowing distinction of false negative PCR findings due to inadequate sample conditions, DNA degradation and/or PCR interfering substances. RESULTS:The novel TaqMan probe and corresponding primers employed in this study improved the analytical sensitivity of the assay to 0.01 par.eq/ml, greater than that attained by previous assays for Tc I and Tc IV strains. The assay was tested in 152 specimens, 35 from 15 different wild reservoir species and 117 from 7 domestic reservoir species, captured in endemic regions of Argentina, Colombia and Mexico and thus potentially infected with different parasite discrete typing units. The eIACs amplified in all samples from domestic reservoirs from Argentina and Mexico, such as Canis familiaris, Felis catus, Sus scrofa, Ovis aries, Equus caballus, Bos taurus and Capra hircus with quantification cycles (Cq's) between 23 and 25. Additionally, the eIACs amplified from samples obtained from wild mammals, such as small rodents Akodon toba, Galea leucoblephara, Rattus rattus, the opossums Didelphis virginiana, D. marsupialis and Marmosa murina, the bats Tadarida brasiliensis, Promops nasutus and Desmodus rotundus, as well as in Conepatus chinga, Lagostomus maximus, Leopardus geoffroyi, Lepus europaeus, Mazama gouazoubira and Lycalopex gymnocercus, rendering Cq's between 24 and 33. CONCLUSIONS:This duplex qPCR assay provides an accurate laboratory tool for screening and quantification of T. cruzi infection in a vast repertoire of domestic and wild mammalian reservoir species, contributing to improve molecular epidemiology studies of T. cruzi transmission cycles.
journal_name
Parasit Vectorsjournal_title
Parasites & vectorsauthors
Wehrendt DP,Gómez-Bravo A,Ramirez JC,Cura C,Pech-May A,Ramsey JM,Abril M,Guhl F,Schijman AGdoi
10.1186/s13071-019-3817-9subject
Has Abstractpub_date
2019-11-29 00:00:00pages
567issue
1issn
1756-3305pii
10.1186/s13071-019-3817-9journal_volume
12pub_type
杂志文章abstract:BACKGROUND:An active immune surveillance and a range of barriers to infection allow the host to effectively eliminate microbial pathogens. However, pathogens may use diverse strategies to subdue such host defences. For instance, one such mechanism is the use of leucine-rich repeat (LRR) proteins by pathogens (microbial...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1842-5
更新日期:2016-10-21 00:00:00
abstract:BACKGROUND:Transmission-blocking vaccines (TBVs) have been considered an important strategy for disrupting the malaria transmission cycle, especially for Plasmodium vivax malaria, which undergoes gametocytogenesis earlier during infection. Pvs25 and Pvs28 are transmission-blocking vaccine candidates for P. vivax malari...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-4-224
更新日期:2011-11-28 00:00:00
abstract:BACKGROUND:With field releases starting in Brazil, particular interest must be given to understanding how the endosymbiotic bacterium Wolbachia pipientis affects Aedes aegypti mosquitoes with a Brazilian genetic background. Currently, there is limited information on how the bacterium affects phenotypic traits such as l...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1559-5
更新日期:2016-05-16 00:00:00
abstract:BACKGROUND:Chagas disease is a major public health concern in Latin America and it is transmitted by insects of the subfamily Triatominae, including Rhodnius spp. Since palm trees are ubiquitous in Colombia and a habitat for Rhodnius spp., the presence of palms near villages could increase contact rates between vectors...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1884-8
更新日期:2016-11-18 00:00:00
abstract:BACKGROUND:During recent periods, the islands of the Republic of Seychelles experienced many diseases such as dengue, chikungunya, Bancroft's filaria and malaria. Mosquitoes transmit the agents that cause these diseases. Published information on mosquitoes in the Seychelles is notably dispersed in the literature. The m...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-5-207
更新日期:2012-09-21 00:00:00
abstract::Schistosomiasis is a major disease of public health importance in humans occurring in 76 countries of the tropics and sub-tropics. In China, schistosomiasis japonica is one of the highest priorities in communicable disease control defined by the central government. Since 1970s, the habitats of Biomphalaria straminea, ...
journal_title:Parasites & vectors
pub_type: 杂志文章,评审
doi:10.1186/1756-3305-6-249
更新日期:2013-08-28 00:00:00
abstract:BACKGROUND:Schistosomiasis japonica remains a major public health concern in China. There are many interventions implemented to control the transmission of the disease. The purpose of the present study was to investigate the effects of an integrated control strategy for schistosomiasis control. METHODS:An integrated c...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-4-243
更新日期:2011-12-30 00:00:00
abstract:BACKGROUND:The present study focuses on both long- and short-term malaria transmission in Eritrea and investigates the risk factors. Annual aggregates of information on malaria cases, deaths, diagnostics and control interventions from 2001 to 2008 and monthly reported data from 2009 to 2017 were obtained from the Natio...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-3974-x
更新日期:2020-03-12 00:00:00
abstract:BACKGROUND:In this study for the first time, a Dientamoeba fragilis protein profile by MALDI-TOF MS was created in order to identify specific markers for the application of this technology in the laboratory diagnosis of dientamoebiasis. In particular, one D. fragilis reference strain was used to create a reference spec...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2597-3
更新日期:2018-01-04 00:00:00
abstract:BACKGROUND:Schistosoma mansoni cercariae penetrate the skin by releasing excretory/secretory (E/S) products known as 0-3hRP, which are associated with immune modulation through Toll like receptor (TLR) signalling. Furthermore, these secretions contain Sm16, which when given to cells as a recombinant protein inhibits hu...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-014-0608-1
更新日期:2015-01-06 00:00:00
abstract:BACKGROUND:Lymphatic filariasis (LF) is a mosquito-borne parasitic disease and a major cause of disability worldwide. It is one of the neglected tropical diseases identified by the World Health Organization for elimination as a public health problem by 2020. Maps displaying disease distribution are helpful tools to ide...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-3097-9
更新日期:2018-09-17 00:00:00
abstract:BACKGROUND:Annual community-directed treatment with ivermectin (CDTI) have been carried out since 1999 in the Lekie division (central region of Cameroon where most cases of Loa-related post ivermectin severe adverse events were reported) as part of the joined activities of the African Programme for Onchocerciasis Contr...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-6-283
更新日期:2013-09-27 00:00:00
abstract:BACKGROUND:Triatomines are blood-sucking vectors of Trypanosoma cruzi, the causative agent of Chagas disease. During feeding, triatomines surpass the skin host response through biomolecules present in their saliva. Dendritic cells (DCs) play a crucial role in the induction of the protection to aggressive agents, includ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1890-x
更新日期:2016-12-09 00:00:00
abstract:BACKGROUND:Malaria remains a major public health concern. Vector control measures based solely on insecticide treated nets (ITNs) and indoor residual spraying (IRS) have demonstrated not to be feasible for malaria elimination. It has been shown that ivermectin affects several aspects of Anopheles species biology. Along...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2563-0
更新日期:2017-12-28 00:00:00
abstract:BACKGROUND:Thymidylate synthase is a housekeeping gene, designated ancient due to its role in DNA synthesis and ubiquitous phyletic distribution. The genomic sequences were characterized coding for thymidylate synthase in two species of the genus Trichinella, an encapsulating T. spiralis and a non-encapsulating T. pseu...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-175
更新日期:2014-04-09 00:00:00
abstract:BACKGROUND:The current understanding of the landscape epidemiology of Ross River virus (RRV), Australia's most common arthropod-borne pathogen, is fragmented due to gaps in surveillance programs and the relatively narrow focus of the research conducted to date. This leaves public health agencies with an incomplete unde...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-2776-x
更新日期:2018-03-20 00:00:00
abstract:BACKGROUND:Leishmania infantum is a vector-borne pathogen endemic in countries in the Mediterranean basin, including Italy. Dogs act as the primary reservoir for this parasite, but other animal species may also be infected. Low-to-moderate seroprevalence levels of infection have been reported in apparent healthy equine...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04385-8
更新日期:2020-10-22 00:00:00
abstract:BACKGROUND:The Culex pipiens complex (Diptera: Culicidae) includes the most widespread mosquito species in the world. Members of this complex are the primary enzootic and epidemic vectors of the West Nile virus (genus Flavivirus) in several countries. The two recognized forms of Cx. pipiens (Linnaeus, 1758) - pipiens a...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2265-7
更新日期:2017-07-10 00:00:00
abstract:BACKGROUND:Pyrenean chamois (Rupicapra pyrenaica pyrenaica) is a nearly monomorphic mountain ungulate with an unbiased sex-specific overwinter adult survival. Few differences in gastrointestinal parasitism have been reported by coprology as yet. This study aims to assess diversity, prevalence, intensity of infection an...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-0774-9
更新日期:2015-03-18 00:00:00
abstract:BACKGROUND:Lipophosphoglycan (LPG) is a dominant surface molecule of Leishmania promastigotes. Its species-specific polymorphisms are found mainly in the sugars that branch off the conserved Gal(β1,4)Man(α1)-PO4 backbone of repeat units. Leishmania amazonensis is one of the most important species causing human cutaneou...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2568-8
更新日期:2017-12-16 00:00:00
abstract:BACKGROUND:Correct species identification of blow flies is a crucial step for understanding their biology, which can be used not only for designing fly control programs, but also to determine the minimum time since death. Identification techniques are usually based on morphological and molecular characters. However, th...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2163-z
更新日期:2017-05-10 00:00:00
abstract:BACKGROUND:Ixodes ricinus is the most common tick species in Europe and the main vector for Borrelia burgdorferi (sensu lato) and tick-borne encephalitis virus (TBEV). It is involved also in the transmission of Borrelia miyamotoi, a relapsing fever spirochete that causes health disorders in humans. Little is known rega...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-3969-7
更新日期:2020-02-27 00:00:00
abstract:BACKGROUND:Several studies were carried out in experimental hut station in areas surrounding the city of Bouaké, after the crisis in Côte d'Ivoire. They reported increasing resistance levels to insecticide for malaria transmiting mosquitoes. The present work aims to evaluate the current resistance level of An. gambiae ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-2702-2
更新日期:2018-03-02 00:00:00
abstract:BACKGROUND:Publications are often used as a measure of success of research work. Leishmaniasis is considered endemic in 98 countries, most of which are developing. This article describes a bibliometric review of the literature on leishmaniasis research indexed in PubMed during a 66-year period. METHODS:Medline was use...
journal_title:Parasites & vectors
pub_type: 杂志文章,评审
doi:10.1186/1756-3305-6-55
更新日期:2013-03-07 00:00:00
abstract:BACKGROUND:Studies of avian haemosporidians allow understanding how these parasites affect wild bird populations, and if their presence is related to factors such as habitat loss, degradation and fragmentation, and climate change. Considering the importance of the highland Plateau of Mexico as part of the North America...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1569-3
更新日期:2016-05-27 00:00:00
abstract:BACKGROUND:Insecticide treated plastic sheeting (ITPS), sometimes known as durable lining, has potential as a long-lasting insecticidal surface for malaria vector control when used as lining for interior walls and ceilings inside the home. Against a backdrop of increasing long lasting net (LN) coverage, we examined the...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-3-65
更新日期:2010-08-03 00:00:00
abstract:BACKGROUND:Mutation of the voltage-gated sodium channel (VGSC) gene, or knockdown resistance (kdr) gene, is an important resistance mechanism against DDT and pyrethroids for dengue vector Aedes albopictus. A phenylalanine to serine (F1534S), leucine (F1534L) and cysteine (F1534C) substitution were detected in many Ae. ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3829-5
更新日期:2019-12-26 00:00:00
abstract:BACKGROUND:Merozoite proteins of the malaria parasites involved in the invasion of red blood cells are selected by host immunity and their diversity is greatly influenced by changes in malaria epidemiology. In the Greater Mekong Subregion (GMS), malaria transmission is concentrated along the international borders and t...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3803-2
更新日期:2019-11-20 00:00:00
abstract:BACKGROUND:Arboviruses are a growing public health concern in Europe, with both endemic and exotic arboviruses expected to spread further into novel areas in the next decades. Predicting where future outbreaks will occur is a major challenge, particularly for regions where these arboviruses are not endemic. Spatial mod...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04339-0
更新日期:2020-09-10 00:00:00
abstract:BACKGROUND:Cutaneous leishmaniasis (CL) is caused by Leishmania (Viannia) braziliensis, which infects dermal macrophages and dendritic cells, causing an intense immune-mediated-tissue inflammation and a skin ulcer with elevated borders that can heal spontaneously or after antimonial therapy. The resolution of lesions d...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2152-2
更新日期:2017-05-03 00:00:00