Abstract：
BACKGROUND:Cathepsin cysteine proteases play multiple roles in the life cycle of parasites such as food uptake, immune invasion and pathogenesis, making them valuable targets for diagnostic assays, vaccines and drugs. The purpose of this study was to identify a cathepsin B of Clonorchis sinensis (CsCB) and to investigate its diagnostic value for human helminthiases. RESULTS:The predicted amino acid sequence of the cathepsin B of C. sinensis shared 63%, 52%, 50% identity with that of Schistosoma japonicum, Homo sapiens and Fasciola hepatica, respectively. Sequence encoding proenzyme of CsCB was overexpressed in Escherichia coli. Reverse transcription PCR experiments revealed that CsCB transcribed in both adult worm and metacercaria of C. sinensis. CsCB was identified as a C. sinensis excretory/secretory product by immunoblot assay, which was consistent with immunohistochemical localization showing that CsCB was especially expressed in the intestine of C. sinensis adults. Both ELISA and western blotting analysis showed recombinant CsCB could react with human sera from clonorchiasis and other helminthiases. CONCLUSIONS:Our findings revealed that secreted CsCB may play an important role in the biology of C. sinensis and could be a diagnostic candidate for helminthiases.
journal_name
Parasit Vectorsjournal_title
Parasites & vectorsauthors
Chen W,Wang X,Li X,Lv X,Zhou C,Deng C,Lei H,Men J,Fan Y,Liang C,Yu Xdoi
10.1186/1756-3305-4-149subject
Has Abstractpub_date
2011-07-27 00:00:00pages
149issn
1756-3305pii
1756-3305-4-149journal_volume
4pub_type
杂志文章abstract：BACKGROUND:Culex pipiens pipiens plays an important role in the transmission of several vector-borne pathogens such as West Nile virus (WNV) in North America. Laboratory and field studies suggest that this species is ornithophilic but because of genetic hybridization with sibling species during the active mosquito seas...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-015-1012-1
更新日期：2015-07-24 00:00:00
abstract：BACKGROUND:Toxoplasma gondii infections and cases of clinical toxoplasmosis have been recorded in zoo animals. Wild felids in human care can serve as definitive hosts that shed oocysts, but also as intermediate hosts for the parasite. Some felid species, such as the Pallas's cat (Otocolobus manul) or sand cat (Felis ma...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-019-3706-2
更新日期：2019-09-11 00:00:00
abstract：BACKGROUND:Cats appear to be the primary reservoir host for Bartonella koehlerae, an alpha Proteobacteria that is most likely transmitted among cat populations by fleas (Ctenocephalides felis). Bartonella koehlerae has caused endocarditis in a dog and in one human patient from Israel, but other clinically relevant repo...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-3-76
更新日期：2010-08-24 00:00:00
abstract：BACKGROUND:Hemotropic mycoplasmas are epicellular erythrocytic bacteria that can cause infectious anemia in some mammalian species. Worldwide, hemotropic mycoplasmas are emerging or re-emerging zoonotic pathogens potentially causing serious and significant health problems in wildlife. The objective of this study was to...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-7-117
更新日期：2014-03-24 00:00:00
abstract：BACKGROUND:Th1, Th2, Th17, Treg and Tfh cells play important roles in schistosomiasis. Th9 cells secrete IL-9 as a signature cytokine and contribute to several classes of inflammatory disease. However, the effects of Th9 cells in schistosomiasis are unknown. We aimed to explore the dynamic changes and potential roles o...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-017-2242-1
更新日期：2017-06-24 00:00:00
abstract：BACKGROUND:An accurate diagnosis of soil-transmitted helminthiasis is important for individual patient management, for drug efficacy evaluation and for monitoring control programmes. The Kato-Katz technique is the most widely used method detecting soil-transmitted helminth eggs in faecal samples. However, detailed anal...
journal_title：Parasites & vectors
pub_type： 杂志文章,随机对照试验
doi：10.1186/s13071-015-0702-z
更新日期：2015-02-05 00:00:00
abstract：BACKGROUND:Despite intensive control measures including governmental campaigns using highly-efficacious systemic insecticides, there is evidence for persisting or recurring bovine Hypoderma species populations in parts of Europe, the USA and Canada. The present study evaluated the efficacy of LONGRANGE® (eprinomectin 5...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-019-3475-y
更新日期：2019-05-14 00:00:00
abstract：BACKGROUND:Bluetongue virus (BTV) is transmitted by Culicoides biting midges and causes bluetongue (BT), a clinical disease observed primarily in sheep. BT has a detrimental effect on subsistence farmers in India, where hyperendemic outbreaks impact on smallholdings in the southern states of the country. In this study,...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-017-1992-0
更新日期：2017-01-31 00:00:00
abstract：BACKGROUND:With the spread of pyrethroid resistance in mosquitoes, the combination of an insecticide (carbamate or organophosphate) with a repellent (DEET) is considered as a promising alternative strategy for the treatment of mosquito nets and other relevant materials. The efficacy of these mixtures comes from the fac...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-2-17
更新日期：2009-04-16 00:00:00
abstract：BACKGROUND:Dirofilaria immitis is a mosquito-borne pathogen that is spreading worldwide, and the associated infection (i.e. dirofilariosis) is becoming a threat to animals and humans living in endemic areas. Little is known about the occurrence and risk of infection of D. immitis in pinnipeds. Here we report dirofilari...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-017-2073-0
更新日期：2017-03-13 00:00:00
abstract：BACKGROUND:The G subfamily of ABC (ATP-binding cassette) transporters of Leishmania include 6 genes (ABCG1-G6), some with relevant biological functions associated with drug resistance and phospholipid transport. Several studies have shown that Leishmania LABCG2 transporter plays a role in the exposure of phosphatidylse...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-017-2198-1
更新日期：2017-05-30 00:00:00
abstract：BACKGROUND:Rhipicephalus sanguineus is a ubiquitous tick responsible for transmitting Ehrlichia canis and most likely Anaplasma platys to dogs, as either single or co-infections. The objective of this study was to assess the effects of either simultaneous or sequential experimental infections with E. canis and A. platy...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-3-33
更新日期：2010-04-08 00:00:00
abstract：BACKGROUND:Domestic cats play a key role in the epidemiology of the parasite Toxoplasma gondii by excreting environmentally-resistant oocysts that may infect humans and other warm-blooded animals. The dynamics of Toxoplasma gondii seroconversion, used as a proxy for primo-infection dynamics, was investigated in five ca...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-018-2834-4
更新日期：2018-06-08 00:00:00
abstract：BACKGROUND:Bravecto (fluralaner; MSD Animal Health) is a novel systemic ectoparasiticide for dogs providing long-acting flea- and tick-control after a single oral dose. Scalibor Protectorband (deltamethrin; MSD Animal Health) is a collar often used to reduce sandfly feeding for leishmaniasis prevention. This study inve...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-7-105
更新日期：2014-03-19 00:00:00
abstract：BACKGROUND:Lipophosphoglycan (LPG) is a dominant surface molecule of Leishmania promastigotes. Its species-specific polymorphisms are found mainly in the sugars that branch off the conserved Gal(β1,4)Man(α1)-PO4 backbone of repeat units. Leishmania amazonensis is one of the most important species causing human cutaneou...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-017-2568-8
更新日期：2017-12-16 00:00:00
abstract：BACKGROUND:The myxosporean Myxidium giardi Cépède, 1906 was described infecting the kidney of the European eel, Anguilla anguilla (L.), having spindle-shaped myxospores and terminal sub-spherical polar capsules. Since then, numerous anguillid eels globally have been documented to have similar Myxidium infections. Many ...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-018-3087-y
更新日期：2018-10-22 00:00:00
abstract：BACKGROUND:Leishmaniasis represents an important public health problem in Brazil. The continuous process of urbanization and expansion of human activities in forest areas impacts natural habitats, modifying the ecology of some species of Leishmania, as well as its vectors and reservoirs and, consequently, changes the e...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-018-2742-7
更新日期：2018-03-07 00:00:00
abstract：BACKGROUND:Ticks are increasingly reported on cats worldwide, with Ixodes ricinus being a relevant species across Europe and in near by areas of North Africa and the Middle East. Yet there are few acaracidal products with proven efficacy approved for use in cats. The objective of this study was to compare the efficacy ...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-017-2259-5
更新日期：2017-06-29 00:00:00
abstract：BACKGROUND:Small mammals are crucial for the life history of ixodid ticks, but their role and importance in the transmission cycle of tick-borne pathogens is mostly unknown. Candidatus Neoehrlichia mikurensis (CNM) and Anaplasma phagocytophilum are both tick-borne pathogens, and rodents are discussed to serve as main r...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-014-0563-x
更新日期：2014-12-04 00:00:00
abstract：BACKGROUND:Canine vector-borne diseases (CVBD) have become a major concern for canine and human public health. The aim of the study described here is to add epidemiological data regarding four pathogens responsible for CVBD, namely anaplasmosis, borreliosis, dirofilariosis and ehrlichiosis in a national survey conducte...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-016-1686-z
更新日期：2016-07-29 00:00:00
abstract：BACKGROUND:Thymidylate synthase is a housekeeping gene, designated ancient due to its role in DNA synthesis and ubiquitous phyletic distribution. The genomic sequences were characterized coding for thymidylate synthase in two species of the genus Trichinella, an encapsulating T. spiralis and a non-encapsulating T. pseu...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-7-175
更新日期：2014-04-09 00:00:00
abstract：BACKGROUND:The action of chemical compounds on the Palaearctic tick D. reticulatus (Fabricius) (Acari: Amblyomminae) has been poorly investigated so far. Therefore, the effects of application of permethrin on engorged D. reticulatus females have been assessed, and the survival rate for the different developmental stage...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-7-18
更新日期：2014-01-10 00:00:00
abstract：BACKGROUND:Liver fluke (Fasciola hepatica) is a widespread parasite of ruminants which can have significant economic impact on cattle production. Fluke infection status at the animal-level is captured during meat inspection of all animals processed for human consumption within Northern Ireland. These national datasets ...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-016-1489-2
更新日期：2016-04-14 00:00:00
abstract：BACKGROUND:The East Route Project (ERP) of the South-to-North Water Diversion Project (SNWDP) stretches across schistosomiasis endemic and non-endemic areas in China, which may lead to the dispersal of Oncomelania hupensis, the intermediate host of Schistosoma japonicum, from permissive areas along the Yangtze River Ba...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-020-4004-8
更新日期：2020-03-19 00:00:00
abstract：BACKGROUND:Taenia ovis is a tapeworm that is mainly transmitted between dogs and sheep. Although T. ovis infection is not a public health issue, it causes a great financial loss due to condemnation of carcasses. The first outbreak of T. ovis infection in China occurred in 2015. Reassessment of adverse effects of T. ov...
journal_title：Parasites & vectors
pub_type： 信件
doi：10.1186/s13071-016-1700-5
更新日期：2016-07-26 00:00:00
abstract：BACKGROUND:Insect-borne diseases could induce severe symptoms in human and clinical signs in animals, such as febrility, erythra, arthralgia and hemorrhagic fever, and cause significant economic losses and pose public health threat all over the world. The significant advantages of Luminex xMAP technology are high-throu...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-018-2996-0
更新日期：2018-07-31 00:00:00
abstract：BACKGROUND:Hepatocellular carcinoma-associated antigen 59 (HCA59), which is one of the most important excretory/secretory products of Haemonchus contortus (HcESPs), is known to have antigenic functions. However, its immunomodulatory effects on host cells are poorly understood. METHODS:Here, we cloned the HCA59 gene an...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-019-3375-1
更新日期：2019-03-14 00:00:00
abstract：BACKGROUND:Tick-borne infections are no longer confined to rural areas, they are documented with increasing frequency in urban settlements across the world. They are known to cause diseases in humans as well as in their companion animals. METHODS:During a period of 2 years, from January 2013 until December 2014, ticks...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-016-1632-0
更新日期：2016-06-21 00:00:00
abstract：BACKGROUND:Leishmania infantum and Toxoplasma gondii are protozoa with zoonotic and economic importance. Prevalences of antibodies to these agents were assessed in 173 horses from the north of Portugal. FINDINGS:Antibodies to L. infantum were detected by the direct agglutination test (DAT); seven (4.0%) horses were se...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-6-178
更新日期：2013-06-17 00:00:00
abstract：BACKGROUND:The transformation of noninfective epimastigotes into infective metacyclic trypomastigotes (metacyclogenesis) is a fundamental step in the life cycle of Trypanosoma cruzi, comprising several morphological and biochemical changes. GP82 and GP90 are glycoproteins expressed at the surface of metacyclic trypomas...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-6-127
更新日期：2013-05-01 00:00:00
