Beta-endorphin-like immunoreactivity levels in the hypothalamus, the periaqueductal grey and the pituitary of the DBA mouse: determination by ELISA and relationship to nociception.

Abstract:

:The present paper describes the development and application of an enzyme-linked immunosorbent assay (ELISA) for the assessment of beta-endorphin-like immunoreactivity (beta-ELIR) level in the hypothalamus, the periaqueductal grey (PAG) and the pituitary of DBA/2 mice that were subjected to mild social stress (aggressive confrontation). After confrontation these subjects showed elevated tail-flick latencies (TFL) when compared to controls, a finding that indicates stress-induced analgesia (SIA). A positive correlation was found between individual TFLs and beta-ELIR levels in the PAG but not in the hypothalamus and the pituitary. These results suggest that individual baseline PAG beta-ELIR levels may be taken as a predictor of high degrees of stress-induced analgesia.

journal_name

Physiol Behav

journal_title

Physiology & behavior

authors

Külling P,Siegfried B,Frischknecht HR,Messiha FS,Pasi A

doi

10.1016/0031-9384(89)90315-6

subject

Has Abstract

pub_date

1989-07-01 00:00:00

pages

25-8

issue

1

eissn

0031-9384

issn

1873-507X

pii

0031-9384(89)90315-6

journal_volume

46

pub_type

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