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Inhibition by rhodamine 123 of protein synthesis in mitochondria of normal and cancer tissues.

Abstract：

:The effect of the mitochondrial dye rhodamine 123 (Rho 123) on protein synthesis (PS) activity was investigated in mitochondria isolated from liver and from both chloroma and erythroleukemia tumors. Incorporation of labelled leucine into mitochondrial protein was used to measure the rate of PS. While PS specific activity was much higher in hematopoietic tumors mitochondria as compared to that of liver, the addition of increased concentration of Rho 123 in all tested organelles resulted in increased inhibition of PS to reach 75-82% with 10 micrograms/ml of the dye. Similar results were obtained with 10 micrograms/ml of chloramphenicol, the specific inhibitor of mitochondrial PS. Moreover, under the conditions of the study, the addition of Rho 123 to mitochondria did not trigger any ATPase activity, thus eliminating any competition for the energy source ATP between PS and ATPase. These results demonstrate that, in addition to its known inhibitory action on oxidative phosphorylation, the mitochondrial dye Rho 123 has a potent inhibitory effect on PS in both liver and hematopoietic tumors mitochondria.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Abou-Khalil WH,Arimura GK,Yunis AA,Abou-Khalil S

doi

10.1016/0006-291x(86)91144-7

subject

Has Abstract

pub_date

1986-06-13 00:00:00

pages

759-65

issue

2

eissn

0006-291X

issn

1090-2104

pii

0006-291X(86)91144-7

journal_volume

137

pub_type

杂志文章

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