Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach.


:Diesel fuel is one of the most important sources of hydrocarbon contamination worldwide. Its composition consists of a complex mixture of n-alkanes, branched alkanes and aromatic compounds. Hydrocarbon degradation in Pseudomonas species has been mostly studied under aerobic conditions; however, a dynamic spectrum of oxygen availability can be found in the environment. Pseudomonas extremaustralis, an Antarctic bacterium isolated from a pristine environment, is able to degrade diesel fuel and presents a wide microaerophilic metabolism. In this work RNA-deep sequence experiments were analyzed comparing the expression profile in aerobic and microaerophilic cultures. Interestingly, genes involved in alkane degradation, including alkB, were over-expressed in micro-aerobiosis in absence of hydrocarbon compounds. In minimal media supplemented with diesel fuel, n-alkanes degradation (C13-C19) after 7 days was observed under low oxygen conditions but not in aerobiosis. In-silico analysis of the alkB promoter zone showed a putative binding sequence for the anaerobic global regulator, Anr. Our results indicate that some diesel fuel components can be utilized as sole carbon source under microaerophilic conditions for cell maintenance or slow growth in a Pseudomonas species and this metabolism could represent an adaptive advantage in polluted environments.


Tribelli PM,Rossi L,Ricardi MM,Gomez-Lozano M,Molin S,Raiger Iustman LJ,Lopez NI




Has Abstract


2018-01-01 00:00:00














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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Khouiti Z,Simon JP

    更新日期:1997-07-01 00:00:00

  • Application of acetate buffer in pH adjustment of sorghum mash and its influence on fuel ethanol fermentation.

    abstract::A 2 M sodium acetate buffer at pH 4.2 was tried to simplify the step of pH adjustment in a laboratory dry-grind procedure. Ethanol yields or conversion efficiencies of 18 sorghum hybrids improved significantly with 2.0-5.9% (3.9% on average) of relative increases when the method of pH adjustment changed from tradition...

    journal_title:Journal of industrial microbiology & biotechnology

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    authors: Zhao R,Bean SR,Crozier-Dodson BA,Fung DY,Wang D

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  • A novel osmolality-shift fermentation strategy for improving acarbose production and concurrently reducing byproduct component C formation by Actinoplanes sp. A56.

    abstract::Component C (Acarviosy-1,4-Glc-1,1-Glc) was a highly structural acarbose analog, which could be largely formed during acarbose fermentation process, resulting in acarbose purification being highly difficult. By choosing osmolality level as the key fermentation parameter of acarbose-producing Actinoplanes sp. A56, this...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Cheng X,Peng WF,Huang L,Zhang B,Li KT

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    journal_title:Journal of industrial microbiology & biotechnology

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    authors: Noble R,Hobbs PJ,Mead A,Dobrovin-Pennington A

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  • Solar Pond devices: free energy or bioreactors for Artemia biomass production?

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    journal_title:Journal of industrial microbiology & biotechnology

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    authors: Gouveia L,Sousa J,Marques A,Tavares C,Giestas M

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  • Supplementation requirements of brewery's spent grain hydrolysate for biomass and xylitol production by Debaryomyces hansenii CCMI 941.

    abstract::The effect of nutrient supplementation of brewery's spent grain (BSG) hydrolysates was evaluated with respect to biomass and xylitol production by Debaryomyces hansenii. For optimal biomass production, supplementation of full-strength BSG hydrolysates required only phosphate (0.5 g l(-1) KH(2)PO(4)), leading to a biom...

    journal_title:Journal of industrial microbiology & biotechnology

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    authors: Carvalheiro F,Duarte LC,Lopes S,Parajó JC,Pereira H,Gírio FM

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  • Phytostimulation and biofertilization in wheat by cyanobacteria.

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    journal_title:Journal of industrial microbiology & biotechnology

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    authors: Hussain A,Hasnain S

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  • Cutinase production by Fusarium oxysporum in liquid medium using central composite design.

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    journal_title:Journal of industrial microbiology & biotechnology

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    authors: Pio TF,Macedo GA

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    journal_title:Journal of industrial microbiology & biotechnology

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    authors: Papagianni M,Joshi N,Moo-Young M

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    authors: Jouzani GS,Abad AP,Seifinejad A,Marzban R,Kariman K,Maleki B

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Wang Z,Xu JH,Chen D

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    authors: Tang CJ,Zheng P,Mahmood Q,Chen JW

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    journal_title:Journal of industrial microbiology & biotechnology

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    authors: Li X,Yu HY

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    authors: Butler AR,Cundliffe E

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    authors: Frykman S,Tsuruta H,Galazzo J,Licari P

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    authors: Manzo RM,de Sousa M,Fenoglio CL,Gonçalves LR,Mammarella EJ

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    pub_type: 杂志文章,评审


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    journal_title:Journal of industrial microbiology & biotechnology

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    authors: Chen K,Liu S,Wang G,Zhang D,Du G,Chen J,Shi Z

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Van As H,Lens P

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  • Insights from the complete genome sequence of Clostridium tyrobutyricum provide a platform for biotechnological and industrial applications.

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Wu Q,Liu T,Zhu L,Huang H,Jiang L

    更新日期:2017-08-01 00:00:00

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    journal_title:Journal of industrial microbiology & biotechnology

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    authors: Prieto-Samsónov DL,Vázquez-Padrón RI,Ayra-Pardo C,González-Cabrera J,de la Riva GA

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Bera AK,Ho NW,Khan A,Sedlak M

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  • Bacterial contaminants of fuel ethanol production.

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Skinner KA,Leathers TD

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Costa SG,Lépine F,Milot S,Déziel E,Nitschke M,Contiero J

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Canilha L,Santos VT,Rocha GJ,Almeida e Silva JB,Giulietti M,Silva SS,Felipe MG,Ferraz A,Milagres AM,Carvalho W

    更新日期:2011-09-01 00:00:00

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Zhan M,Kan B,Dong J,Xu G,Han R,Ni Y

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Zhao B,He YL,Huang J,Taylor S,Hughes J

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