Evolving enzyme technology for pharmaceutical applications: case studies.


:The case studies focus on two types of enzyme applications for pharmaceutical development. Demethylmacrocin O-methyltransferase, macrocin O-methyltransferase (both putatively rate-limiting) and tylosin reductase were purified from Streptomyces fradiae, characterized and the genes manipulated for increasing tylosin biosynthesis in S. fradiae. The rate-limiting enzyme, deacetoxycephalosporin C (DAOC) synthase/hydroxylase (expandase/ hydroxylase), was purified from Cephalosporium acremonium, its gene over-expressed, and cephalosporin C biosynthesis improved in C. acremonium. Also, heterologous expression of penicillin N epimerase and DAOC synthase (expandase) genes of Streptomyces clavuligerus in Penicillium chrysogenum permitted DAOC production in the fungal strain. Second, serine hydroxymethyltransferase of Escherichia coli and phthalyl amidase of Xanthobacter agilis were employed in chemo-enzymatic synthesis of carbacephem. Similarly, echinocandin B deacylase of Actinoplanes utahensis was used in the second-type synthesis of the ECB antifungal agent.


Yeh WK




Has Abstract


1997-11-01 00:00:00












  • Comparative analysis of the Corynebacterium glutamicum transcriptome in response to changes in dissolved oxygen levels.

    abstract::The dissolved oxygen (DO) level of a culture of Corynebacterium glutamicum (C. glutamicum) in a bioreactor has a significant impact on the cellular redox potential and the distribution of energy and metabolites. In this study, to gain a deeper understanding of the effects of DO on the metabolism of C. glutamicum, we s...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,收录出版


    authors: Liu X,Yang S,Wang F,Dai X,Yang Y,Bai Z

    更新日期:2017-02-01 00:00:00

  • INDISIM-Denitrification, an individual-based model for study the denitrification process.

    abstract::Denitrification is one of the key processes of the global nitrogen (N) cycle driven by bacteria. It has been widely known for more than 100 years as a process by which the biogeochemical N-cycle is balanced. To study this process, we develop an individual-based model called INDISIM-Denitrification. The model embeds a ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Araujo-Granda P,Gras A,Ginovart M,Moulton V

    更新日期:2020-01-01 00:00:00

  • Bacterial cell attachment, the beginning of a biofilm.

    abstract::The ability of bacteria to attach to surfaces and develop into a biofilm has been of considerable interest to many groups in numerous industries, including the medical and food industry. However, little is understood in the critical initial step seen in all biofilm development, the initial bacterial cell attachment to...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Palmer J,Flint S,Brooks J

    更新日期:2007-09-01 00:00:00

  • Enhanced thermostability of keratinase by computational design and empirical mutation.

    abstract::Keratinases are proteolytic enzymes capable of degrading insoluble keratins. The importance of these enzymes is being increasingly recognized in fields as diverse as animal feed production, textile processing, detergent formulation, leather manufacture, and medicine. To enhance the thermostability of Bacillus lichenif...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Liu B,Zhang J,Fang Z,Gu L,Liao X,Du G,Chen J

    更新日期:2013-07-01 00:00:00

  • Expression, characterization, and improvement of a newly cloned halohydrin dehalogenase from Agrobacterium tumefaciens and its application in production of epichlorohydrin.

    abstract::A gene encoding halohydrin dehalogenase (HHDH) from Agrobacterium tumefaciens CCTCC M 87071 was cloned and expressed in Escherichia coli. To increase activity and stability of HHDH, 14 amino acid residues around the active site and substrate-binding pocket based on the structural analysis and molecular docking were se...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Liu ZQ,Gao AC,Wang YJ,Zheng YG,Shen YC

    更新日期:2014-07-01 00:00:00

  • A recombinant vaccine against hydatidosis: production of the antigen in Escherichia coli.

    abstract::A commercial process was developed for producing a recombinant vaccine against hydatidosis in farm animals. The vaccine antigen consisting of a surface protein of the oncospheres of the hydatid worm (Echinococcus granulosus), was produced as inclusion bodies in Escherichia coli. Fed-batch cultures of E. coli using Ter...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Manderson D,Dempster R,Chisti Y

    更新日期:2006-03-01 00:00:00

  • Five gene products are required for assembly of the central pyrrole moiety of coumermycin A1.

    abstract::Coumermycin A1 is an aminocoumarin antibiotic produced by Streptomyces rishiriensis. It exhibits potent antibacterial and anticancer activity. The coumermycin A1 molecule contains two terminal 5-methyl-pyrrole-2-carboxylic acid moieties and one central 3-methylpyrrole-2,4-dicarboxylic acid moiety (CPM). While the bios...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Novotna J,Gust B,Kulik A,Spizek J,Heide L

    更新日期:2013-08-01 00:00:00

  • Production of recombinant herpes simplex virus protease in 10-L stirred vessels using a baculovirus-insect cell expression system.

    abstract::A gene expression system using recombinant Autographa california nuclear polyhedrosis virus (baculovirus) and Sf-9 cells has been scaled up to the 10-L tank level and shown to be capable of producing herpes simplex virus (HSV) protease in serum-free media. High densities of Spodoptera frugiperda (Sf-9) cells were achi...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Schwartz JL,Ferrari EB,Terracciano J,Troyanovich J,Gunnarsson I,Wright-Minogue J,Chen JW,Kwong AD

    更新日期:1997-08-01 00:00:00

  • Corrections to: Enhanced lincomycin production by co‑overexpression of metK1 and metK2 in Streptomyces lincolnensis.

    abstract::In the online published article, row value "pIB139-metK1-metK2" in table 1 has been processed incorrectly. The correct table is given below. ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,已发布勘误


    authors: Xu Y,Tan G,Ke M,Li J,Tang Y,Meng S,Niu J,Wang Y,Liu R,Wu H,Bai L,Zhang L,Zhang B

    更新日期:2018-06-01 00:00:00

  • Contact-free cold atmospheric plasma treatment of Deinococcus radiodurans.

    abstract::In this study we investigated the sensitivity of Deinococcus radiodurans to contact-free cold atmospheric plasma treatment as part of a project to establish new efficient procedures for disinfection of inanimate surfaces. The Gram-positive D. radiodurans is one of the most resistant microorganisms worldwide. Stationar...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Maisch T,Shimizu T,Mitra A,Heinlin J,Karrer S,Li YF,Morfill G,Zimmermann JL

    更新日期:2012-09-01 00:00:00

  • Development of a whole-cell biocatalyst with NADPH regeneration system for biosulfoxidation.

    abstract::A formate dehydrogenase gene (fdh) originated from Candida boidinii was co-expressed in E. coli BL21 (DE3) with the cyclohexanone monooxygenase gene (chmo) cloned from Acinetobacter calcoaceticus NCIMB 9871. The co-expression system was then used as a whole-cell biocatalyst to synthesize chiral phenyl methyl sulfoxide...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Zhai XH,Ma YH,Lai DY,Zhou S,Chen ZM

    更新日期:2013-08-01 00:00:00

  • Investigation of secondary metabolism in the industrial butanol hyper-producer Clostridium saccharoperbutylacetonicum N1-4.

    abstract::Clostridium saccharoperbutylacetonicum N1-4 (Csa) is a historically significant anaerobic bacterium which can perform saccharolytic fermentations to produce acetone, butanol, and ethanol (ABE). Recent genomic analyses have highlighted this organism's potential to produce polyketide and nonribosomal peptide secondary m...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Li JS,Barber CC,Herman NA,Cai W,Zafrir E,Du Y,Zhu X,Skyrud W,Zhang W

    更新日期:2020-03-01 00:00:00

  • Engineering of Rhodococcus cell catalysts for tolerance improvement by sigma factor mutation and active plasmid partition.

    abstract::Tolerance to various stresses is a key phenotype for cell catalysts, which are used widely in bioproduction of diverse valuable chemicals. Using the Rhodococcus ruber TH strain, which exhibits high nitrile hydratase activity, as the target cell catalyst for acrylamide production, we established a method to improve cel...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Ma Y,Yu H

    更新日期:2012-10-01 00:00:00

  • Improved glycerol to ethanol conversion by E. coli using a metagenomic fragment isolated from an anaerobic reactor.

    abstract::Crude glycerol obtained as a by-product of biodiesel production is a reliable feedstock with the potential to be converted into reduced chemicals with high yields. It has been previously shown that ethanol is the primary product of glycerol fermentation by Escherichia coli. However, few efforts were made to enhance th...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Loaces I,Rodríguez C,Amarelle V,Fabiano E,Noya F

    更新日期:2016-10-01 00:00:00

  • Antibiotic biosynthesis: from natural to unnatural compounds.

    abstract::The evolution of the field of biosynthesis from the unravelling of the mode of formation of natural products to the use of such knowledge to create new compounds is reviewed using examples from the author's laboratory. The discussion focuses on the mode of operation of type II (spore pigment PKS) and type I (rifamycin...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Floss HG

    更新日期:2001-09-01 00:00:00

  • Genetic manipulation of secondary metabolite biosynthesis for improved production in Streptomyces and other actinomycetes.

    abstract::Actinomycetes continue to be important sources for the discovery of secondary metabolites for applications in human medicine, animal health, and crop protection. With the maturation of actinomycete genome mining as a robust approach to identify new and novel cryptic secondary metabolite gene clusters, it is critical t...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Baltz RH

    更新日期:2016-03-01 00:00:00

  • Improved production of clavulanic acid by reverse engineering and overexpression of the regulatory genes in an industrial Streptomyces clavuligerus strain.

    abstract::Genomic analysis of the clavulanic acid (CA)-high-producing Streptomyces clavuligerus strains, OL13 and OR, developed through random mutagenesis revealed a frameshift mutation in the cas1 gene-encoding clavaminate synthase 1. Overexpression of the intact cas1 in S. clavuligerus OR enhanced the CA titer by approximatel...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Cho HS,Jo JC,Shin CH,Lee N,Choi JS,Cho BK,Roe JH,Kim CW,Kwon HJ,Yoon YJ

    更新日期:2019-08-01 00:00:00

  • Sequential improvement of rimocidin production in Streptomyces rimosus M527 by introduction of cumulative drug-resistance mutations.

    abstract::Rimocidin is a polyene macrolide that exhibits a strong inhibitory activity against a broad range of plant-pathogenic fungi. In this study, fermentation optimization and ribosome engineering technology were employed to enhance rimocidin production in Streptomyces rimosus M527. After the optimization of fermentation, r...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Zhao Y,Song Z,Ma Z,Bechthold A,Yu X

    更新日期:2019-05-01 00:00:00

  • Structural analysis of the curdlan-like exopolysaccharide produced by Cellulomonas flavigena KU.

    abstract::Cellulomonas flavigena KU produces large quantities of an insoluble exopolysaccharide (EPS) under certain growth conditions. The EPS has previously been shown to be a glucose polymer and to have solubility properties similar to curdlan, a beta-1,3-D-glucan produced by Alcaligenes faecalis var. myxogenes 10C3K. Further...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Kenyon WJ,Buller CS

    更新日期:2002-10-01 00:00:00

  • A study on the pretreatment of a sugarcane bagasse sample with dilute sulfuric acid.

    abstract::Experiments based on a 2(3) central composite full factorial design were carried out in 200-ml stainless-steel containers to study the pretreatment, with dilute sulfuric acid, of a sugarcane bagasse sample obtained from a local sugar-alcohol mill. The independent variables selected for study were temperature, varied f...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Canilha L,Santos VT,Rocha GJ,Almeida e Silva JB,Giulietti M,Silva SS,Felipe MG,Ferraz A,Milagres AM,Carvalho W

    更新日期:2011-09-01 00:00:00

  • Deletion of PHO13 improves aerobic L-arabinose fermentation in engineered Saccharomyces cerevisiae.

    abstract::Pentose sugars are increasingly being used in industrial applications of Saccharomyces cerevisiae. Although L-arabinose is a highlighted pentose that has been identified as next-generation biomass, arabinose fermentation has not yet undergone extensive development for industrial utilization. In this study, we integrat...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Ye S,Jeong D,Shon JC,Liu KH,Kim KH,Shin M,Kim SR

    更新日期:2019-12-01 00:00:00

  • Colanic acid is an exopolysaccharide common to many enterobacteria isolated from paper-machine slimes.

    abstract::In this study, polysaccharide-producing bacteria were isolated from slimes collected from two Finnish and one Spanish paper mill and the exopolysaccharides (EPSs) produced by 18 isolates were characterised. Most of the isolates, selected on the bases of slimy colony morphology, were members of the family Enterobacteri...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Rättö M,Verhoef R,Suihko ML,Blanco A,Schols HA,Voragen AG,Wilting R,Siika-Aho M,Buchert J

    更新日期:2006-05-01 00:00:00

  • Biotransformation of benzonitrile herbicides via the nitrile hydratase-amidase pathway in rhodococci.

    abstract::The aim of this work was to determine the ability of rhodococci to transform 3,5-dichloro-4-hydroxybenzonitrile (chloroxynil), 3,5-dibromo-4-hydroxybenzonitrile (bromoxynil), 3,5-diiodo-4-hydroxybenzonitrile (ioxynil) and 2,6-dichlorobenzonitrile (dichlobenil); to identify the products and determine their acute toxici...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Veselá AB,Pelantová H,Sulc M,Macková M,Lovecká P,Thimová M,Pasquarelli F,Pičmanová M,Pátek M,Bhalla TC,Martínková L

    更新日期:2012-12-01 00:00:00

  • A comparison of stress tolerance in YPD and industrial lignocellulose-based medium among industrial and laboratory yeast strains.

    abstract::In general, it is believed that fermentation by yeast under harsh industrial conditions, especially if substrates such as wood hydrolysate or lignocellulosic substrates are used, requires the use of so-called industrial strains. In order to check whether this is always true, a comparison of performance was made using ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Albers E,Larsson C

    更新日期:2009-08-01 00:00:00

  • Transcriptome analysis of probiotic Lactobacillus casei Zhang during fermentation in soymilk.

    abstract::Lactobacillus casei Zhang is a widely recognized probiotic bacterium, which is being commercially used in China. To study the gene expression dynamics of L. casei Zhang during fermentation in soymilk, a whole genome microarray was used to screen for differentially expressed genes when grown to the lag phase, the late ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Wang JC,Zhang WY,Zhong Z,Wei AB,Bao QH,Zhang Y,Sun TS,Postnikoff A,Meng H,Zhang HP

    更新日期:2012-01-01 00:00:00

  • Recombinant carbazole-degrading strains for enhanced petroleum processing.

    abstract::Biotechnological upgrading of fossil fuels is of increasing interest as remaining stocks of petroleum show increasing levels of contaminants such as heavy metals, sulfur and nitrogen-containing heteroaromatic compounds. Carbazole is of particular interest as a major petroleum component known to reduce refining yields ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Riddle RR,Gibbs PR,Willson RC,Benedik MJ

    更新日期:2003-01-01 00:00:00

  • Isolation and characterization of endophytic huperzine A-producing fungi from Huperzia serrata.

    abstract::Huperzia serrata is a producer of huperzine A (HupA), a cholinesterase inhibitor (ChEI). Over 120 endophytic fungi were recovered from this plant and screened for Hup-A and nine were found. These nine represented seven different fungal genera with the most significant producer being Shiraia sp. A total of 127 endophyt...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Wang Y,Zeng QG,Zhang ZB,Yan RM,Wang LY,Zhu D

    更新日期:2011-09-01 00:00:00

  • BEAP profiles as rapid test system for status analysis and early detection of process incidents in biogas plants.

    abstract::A method was developed to quantify the performance of microorganisms involved in different digestion levels in biogas plants. The test system was based on the addition of butyrate (BCON), ethanol (ECON), acetate (ACON) or propionate (PCON) to biogas sludge samples and the subsequent analysis of CH4 formation in compar...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Refai S,Berger S,Wassmann K,Hecht M,Dickhaus T,Deppenmeier U

    更新日期:2017-03-01 00:00:00

  • Construction of advanced producers of first- and second-generation ethanol in Saccharomyces cerevisiae and selected species of non-conventional yeasts (Scheffersomyces stipitis, Ogataea polymorpha).

    abstract::This review summarizes progress in the construction of efficient yeast ethanol producers from glucose/sucrose and lignocellulose. Saccharomyces cerevisiae is the major industrial producer of first-generation ethanol. The different approaches to increase ethanol yield and productivity from glucose in S. cerevisiae are ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Ruchala J,Kurylenko OO,Dmytruk KV,Sibirny AA

    更新日期:2020-01-01 00:00:00

  • In silico aided metabolic engineering of Klebsiella oxytoca and fermentation optimization for enhanced 2,3-butanediol production.

    abstract::Klebsiella oxytoca naturally produces a large amount of 2,3-butanediol (2,3-BD), a promising bulk chemical with wide industrial applications, along with various byproducts. In this study, the in silico gene knockout simulation of K. oxytoca was carried out for 2,3-BD overproduction by inhibiting the formation of bypro...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Park JM,Song H,Lee HJ,Seung D

    更新日期:2013-09-01 00:00:00