Production of beta-glucosidase using immobilised Piromyces sp. KSX1 and Orpinomyces sp. 478P1 in repeat-batch culture.

Abstract:

:Two anaerobic fungi, one a monocentric strain ( Piromyces sp. KSX1) and the other a polycentric strain ( Orpinomyces sp. 478P1), were immobilised in calcium alginate beads and cultured in sequential batches where spent medium (containing 0.25% cellobiose) was repeatedly drained and replaced. beta-Glucosidase production with KSX1 was maintained for 45 days over six repeated batch cultures yielding a maximum level of 107 mIU/ml. For 478P1, beta-glucosidase production was maintained for 30 days over four repeated batches yielding a maximum level of 34 mIU/ml. Although repeat-batch cultures of KSX1 produced more beta-glucosidase than strain 478P1, the maximum specific beta-glucosidase produced from these immobilised cultures was similar. The immobilised polycentric strain proved to be operationally superior to strain KSX1, as strain 478P1 did not produce any growth in the culture liquor.

authors

McCabe BK,Kuek C,Gordon GL,Phillips MW

doi

10.1007/s10295-003-0033-5

subject

Has Abstract

pub_date

2003-04-01 00:00:00

pages

205-9

issue

4

eissn

1367-5435

issn

1476-5535

journal_volume

30

pub_type

杂志文章
  • Bacterial cell attachment, the beginning of a biofilm.

    abstract::The ability of bacteria to attach to surfaces and develop into a biofilm has been of considerable interest to many groups in numerous industries, including the medical and food industry. However, little is understood in the critical initial step seen in all biofilm development, the initial bacterial cell attachment to...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s10295-007-0234-4

    authors: Palmer J,Flint S,Brooks J

    更新日期:2007-09-01 00:00:00

  • Poly(hydroxyalkanoate) synthase genotype and PHA production of Pseudomonas corrugata and P. mediterranea.

    abstract::A collection of Pseudomonas corrugata and P. mediterranea strains, two closely related species, was evaluated for the presence and variability of pha loci. Using PCR methods that specifically amplify segments of medium-chain-length poly(hydroxyalkanoate) (mcl-PHA) synthase genes, we demonstrated the presence of phaC1 ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-005-0205-6

    authors: Solaiman DK,Catara V,Greco S

    更新日期:2005-02-01 00:00:00

  • Characterization of product capture resin during microbial cultivations.

    abstract::Various bioactive small molecules produced by microbial cultivation are degraded in the culture broth or may repress the formation of additional product. The inclusion of hydrophobic adsorber resin beads to capture these products in situ and remove them from the culture broth can reduce or prevent this degradation and...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-006-0088-1

    authors: Frykman S,Tsuruta H,Galazzo J,Licari P

    更新日期:2006-06-01 00:00:00

  • Natural and engineered biosynthesis of nucleoside antibiotics in Actinomycetes.

    abstract::Nucleoside antibiotics constitute an important family of microbial natural products bearing diverse bioactivities and unusual structural features. Their biosynthetic logics are unique with involvement of complex multi-enzymatic reactions leading to the intricate molecules from simple building blocks. Understanding how...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s10295-015-1636-3

    authors: Chen W,Qi J,Wu P,Wan D,Liu J,Feng X,Deng Z

    更新日期:2016-03-01 00:00:00

  • Electron flux and microbial community in microbial fuel cells (open-circuit and closed-circuit modes) and fermentation.

    abstract::A closed-circuit microbial fuel cell (C-MFC) was operated to investigate the electron flux under fed-batch mode, and the results were compared to those of open-circuit MFC (O-MFC) and a fermentation reactor (F-reactor). The current was the largest electron sink (52.7% of influent SCOD) in C-MFC, whereas biomass and me...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-015-1629-2

    authors: Yu J,Park Y,Lee T

    更新日期:2015-07-01 00:00:00

  • Isolation and characterization of endophytic huperzine A-producing fungi from Huperzia serrata.

    abstract::Huperzia serrata is a producer of huperzine A (HupA), a cholinesterase inhibitor (ChEI). Over 120 endophytic fungi were recovered from this plant and screened for Hup-A and nine were found. These nine represented seven different fungal genera with the most significant producer being Shiraia sp. A total of 127 endophyt...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-010-0905-4

    authors: Wang Y,Zeng QG,Zhang ZB,Yan RM,Wang LY,Zhu D

    更新日期:2011-09-01 00:00:00

  • Effects of water potential on spore germination and viability of Fusarium species.

    abstract::Germination of macroconidia and/or microconidia of 24 strains of Fusarium solani, F. chlamydosporum, F. culmorum, F. equiseti, F. verticillioides, F. sambucinum, F. oxysporum and F. proliferatum isolated from fluvial channels and sea beds of the south-eastern coast of Spain, and three control strains (F. oxysporum iso...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-008-0441-7

    authors: Palmero Llamas D,de Cara Gonzalez M,Iglesias Gonzalez C,Ruíz Lopez G,Tello Marquina JC

    更新日期:2008-11-01 00:00:00

  • Gene cloning, expression, and biochemical characterization of an alkali-tolerant β-mannanase from Humicola insolens Y1.

    abstract::In this article, we firstly report a highly alkali-tolerant fungal β-mannanase from Humicola insolens Y1. The full-length cDNA of the β-mannanase, designated as man5A, has an open reading frame of 1,233 bp that encodes a 411-amino acid polypeptide (Man5A) with a calculated molecular mass of 42.3 kDa. The deduced seque...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-011-1067-8

    authors: Luo H,Wang K,Huang H,Shi P,Yang P,Yao B

    更新日期:2012-04-01 00:00:00

  • Eicosapentaenoic and docosahexaenoic acids production by and okara-utilizing potential of thraustochytrids.

    abstract::Nine thraustochytrid strains isolated from subtropical mangroves were screened for their eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) production potential in a glucose yeast extract medium. Their ability to utilize okara (soymilk residue) for growth and EPA and DHA production was also evaluated. EPA yiel...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1038/sj.jim.7000169

    authors: Fan KW,Chen F,Jones EB,Vrijmoed LL

    更新日期:2001-10-01 00:00:00

  • Colanic acid is an exopolysaccharide common to many enterobacteria isolated from paper-machine slimes.

    abstract::In this study, polysaccharide-producing bacteria were isolated from slimes collected from two Finnish and one Spanish paper mill and the exopolysaccharides (EPSs) produced by 18 isolates were characterised. Most of the isolates, selected on the bases of slimy colony morphology, were members of the family Enterobacteri...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-005-0064-1

    authors: Rättö M,Verhoef R,Suihko ML,Blanco A,Schols HA,Voragen AG,Wilting R,Siika-Aho M,Buchert J

    更新日期:2006-05-01 00:00:00

  • Production and single-step purification of Brugia malayi abundant larval transcript (ALT-2) using hydrophobic interaction chromatography.

    abstract::Abundant larval transcript (ALT), a novel filarial protein, has been shown to have great potential as a vaccine in the prevention of human lymphatic filariasis. In this study, we report a method for the production of recombinant ALT-2 protein, expressed in the cytoplasm of bacterium Escherichia coli in soluble form an...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-010-0753-2

    authors: Bhuvanesh S,Arunkumar C,Kaliraj P,Ramalingam S

    更新日期:2010-10-01 00:00:00

  • Bifunctional xylanases and their potential use in biotechnology.

    abstract::Plant cell walls are comprised of cellulose, hemicellulose and other polymers that are intertwined. This complex structure acts as a barrier to degradation by single enzyme. Thus, a cocktail consisting of bi and multifunctional xylanases and xylan debranching enzymes is most desired combination for the efficient utili...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s10295-008-0342-9

    authors: Khandeparker R,Numan MT

    更新日期:2008-07-01 00:00:00

  • The impact of Ivan Málek's continuous culture concept on bioprocessing.

    abstract::This paper summarizes research results and their industrial applications obtained by continuous culture in the former Czechoslovakia. Past achievements as well as recent trends and developments worldwide are presented. The term "Prague School of continuous culture" is put forward and its international activity is outl...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 历史文章,杂志文章

    doi:10.1007/s10295-010-0881-8

    authors: Kyslík P,Prokop A

    更新日期:2010-12-01 00:00:00

  • Novel organization of catechol meta pathway genes in the nitrobenzene degrader Comamonas sp. JS765 and its evolutionary implication.

    abstract::The catechol meta cleavage pathway is one of the central metabolic pathways for the degradation of aromatic compounds. A novel organization of the pathway genes, different from that of classical soil microorganisms, has been observed in Sphingomonas sp HV3 and Pseudomonas sp. DJ77. In a Comamonas sp. JS765, cdoE encod...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-006-0170-8

    authors: He Z,Parales RE,Spain JC,Johnson GR

    更新日期:2007-02-01 00:00:00

  • Characterisation of aerobically grown non-spore-forming bacteria from paper mill pulps containing recycled fibres.

    abstract::A total of 179 non-spore-forming bacteria aerobically growing on Nutrient Agar, Plate Count Agar or in specific enrichment conditions for salmonella, campylobacteria, listeria, yersinia or staphylococci, were isolated from 16 untreated paper mill pulps. After phenotypical screening the isolates were characterised by a...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-008-0472-0

    authors: Suihko ML,Skyttä E

    更新日期:2009-01-01 00:00:00

  • Electrotransformation studies in Clostridium cellulolyticum.

    abstract::Electropermeabilization of Clostridium cellulolyticum was optimized using ATP leakage assays. Electrotransformation was then performed under optimized conditions (6 to 7.5 kV x cm(-1) field strength applied during 5 ms to a mixture containing methylated plasmids and late exponential phase cell suspensions (10 molecule...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1038/sj.jim.7000081

    authors: Tardif C,Maamar H,Balfin M,Belaich JP

    更新日期:2001-11-01 00:00:00

  • Heterologous production of a new lasso peptide brevunsin in Sphingomonas subterranea.

    abstract::A shuttle vector pHSG396Sp was constructed to perform gene expression using Sphingomonas subterranea as a host. A new lasso peptide biosynthetic gene cluster, derived from Brevundimonas diminuta, was amplified by PCR and integrated to afford a expression vector pHSG396Sp-12697L. The new lasso peptide brevunsin was suc...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-018-2077-6

    authors: Kodani S,Hemmi H,Miyake Y,Kaweewan I,Nakagawa H

    更新日期:2018-11-01 00:00:00

  • Analysis of respiratory activity and carbon usage of a mutant of Azotobacter vinelandii impaired in poly-β-hydroxybutyrate synthesis.

    abstract::In this study, the respiratory activity and carbon usage of the mutant strain of A. vinelandii AT6, impaired in poly-β-hydroxybutyrate (PHB) production, and their relationship with the synthesis of alginate were evaluated. The alginate yield and the specific oxygen uptake rate were higher (2.5-fold and 62 %, respectiv...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-016-1774-2

    authors: Jiménez L,Castillo T,Flores C,Segura D,Galindo E,Peña C

    更新日期:2016-08-01 00:00:00

  • Influence of dimethylsulfoxide on tylosin production in Streptomyces fradiae.

    abstract::The polyketide aglycone, tylactone (protylonolide), does not normally accumulate during tylosin production in Streptomyces fradiae, suggesting that the capacity of the organism to glycosylate tylactone exceeds the capacity for polyketide synthesis. Consistent with this model, tylosin yields were significantly increase...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1038/sj.jim.7000160

    authors: Butler AR,Cundliffe E

    更新日期:2001-07-01 00:00:00

  • Synthesis of 4-nitrophenyl acetate using molecular sieve-immobilized lipase from Bacillus coagulans.

    abstract::Extracellular lipase from Bacillus coagulans BTS-3 was immobilized on (3 A x 1.5 mm) molecular sieve. The molecular sieve showed approximately 68.48% binding efficiency for lipase (specific activity 55 IU mg(-1)). The immobilized enzyme achieved approx 90% conversion of acetic acid and 4-nitrophenol (100 mM each) into...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-008-0510-y

    authors: Raghuvanshi S,Gupta R

    更新日期:2009-03-01 00:00:00

  • Improving conjugation efficacy of Sorangium cellulosum by the addition of dual selection antibiotics.

    abstract::The conjugation protocols in myxobacterium Sorangium cellulosum are often inapplicable due to the strain-specific sensitivity to the presence of Escherichia coli cells or the resistances to many antibiotics. Here we report that the conjugative transfer of the mobilizable plasmid pCVD442 from E. coli DH5alpha (lambda p...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-008-0395-9

    authors: Xia ZJ,Wang J,Hu W,Liu H,Gao XZ,Wu ZH,Zhang PY,Li YZ

    更新日期:2008-10-01 00:00:00

  • Assessing the potential of wild yeasts for bioethanol production.

    abstract::Bioethanol fermentations expose yeasts to a new, complex and challenging fermentation medium with specific inhibitors and sugar mixtures depending on the type of carbon source. It is, therefore, suggested that the natural diversity of yeasts should be further exploited in order to find yeasts with good ethanol yield i...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-014-1544-y

    authors: Ruyters S,Mukherjee V,Verstrepen KJ,Thevelein JM,Willems KA,Lievens B

    更新日期:2015-01-01 00:00:00

  • Study of metabolic network of Cupriavidus necator DSM 545 growing on glycerol by applying elementary flux modes and yield space analysis.

    abstract::A metabolic network consisting of 48 reactions was established to describe intracellular processes during growth and poly-3-hydroxybutyrate (PHB) production for Cupriavidus necator DSM 545. Glycerol acted as the sole carbon source during exponential, steady-state cultivation conditions. Elementary flux modes were obta...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-014-1439-y

    authors: Lopar M,Špoljarić IV,Cepanec N,Koller M,Braunegg G,Horvat P

    更新日期:2014-06-01 00:00:00

  • Lipidomics characterization of the alterations of Trichoderma brevicompactum membrane glycerophospholipids during the fermentation phase.

    abstract::The biological membrane lipid composition has been demonstrated to greatly influence the secretion of secondary metabolites. This study was conducted to investigate the periodical alterations of whole cellular lipids and their associations with secondary products in Trichoderma brevicompactum. An electrospray ionizati...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-019-02152-y

    authors: Bai Y,Gao Y,Lu X,Wang H

    更新日期:2019-06-01 00:00:00

  • Molecular and phenotypic comparison of phaeochromycin-producing strains of Streptomyces phaeochromogenes and Streptomyces ederensis.

    abstract::Streptomyces strain LL-P018 produces the phaeochromycins, novel anti-inflammatory polyketides. This organism was identified as a strain of Streptomyces phaeochromogenes by physiological and genetic taxonomic analysis. In order to gain greater taxonomic perspective, LL-P018 was compared to related strains from major cu...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-008-0367-0

    authors: Ritacco FV,Eveleigh DE

    更新日期:2008-09-01 00:00:00

  • Cometabolic reduction of bromate by a mixed culture of microorganisms using hydrogen gas in a gas-lift reactor.

    abstract::The discharge of bromate, a suspected carcinogen, will be restricted in the near future. To assess the possibility of biotechnological treatment of bromate-containing wastewaters, the removal of bromate by chlorate-reducing microorganisms was studied. The removal of bromate and chlorate was studied in laboratory gas-l...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-004-0188-8

    authors: van Ginkel CG,Middelhuis BJ,Spijk F,Abma WR

    更新日期:2005-01-01 00:00:00

  • The performance of fungal xylan-degrading enzyme preparations in elemental chlorine-free bleaching for Eucalyptus pulp.

    abstract::Cellulase-free xylan-degrading enzyme preparations from Acrophialophora nainiana, Humicola grisea var. thermoidea and two Trichoderma harzianum strains were used as bleaching agents for Eucalyptus kraft pulp, prior to a chlorine dioxide and alkaline bleaching sequence. In comparison to the control sequence (performed ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1038/sj/jim/7000227

    authors: Medeiros RG,Silva FG Jr,Salles BC,Estelles RS,Filho EX

    更新日期:2002-04-01 00:00:00

  • Construction of the astaxanthin biosynthetic pathway in a methanotrophic bacterium Methylomonas sp. strain 16a.

    abstract::Methylomonas sp. strain 16a is an obligate methanotrophic bacterium that uses methane or methanol as the sole carbon source. An effort was made to engineer this organism for astaxanthin production. Upon expressing the canthaxanthin gene cluster under the control of the native hps promoter in the chromosome, canthaxant...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-006-0197-x

    authors: Ye RW,Yao H,Stead K,Wang T,Tao L,Cheng Q,Sharpe PL,Suh W,Nagel E,Arcilla D,Dragotta D,Miller ES

    更新日期:2007-04-01 00:00:00

  • Insights from the complete genome sequence of Clostridium tyrobutyricum provide a platform for biotechnological and industrial applications.

    abstract::Genetic research enables the evolution of novel biochemical reactions for the production of valuable chemicals from environmentally-friendly raw materials. However, the choice of appropriate microorganisms to support these reactions, which must have strong robustness and be capable of a significant product output, is ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-017-1956-6

    authors: Wu Q,Liu T,Zhu L,Huang H,Jiang L

    更新日期:2017-08-01 00:00:00

  • Isolation and characterization of a new bacteriocin, termed enterocin M, produced by environmental isolate Enterococcus faecium AL41.

    abstract::The new bacteriocin, termed enterocin M, produced by Enterococcus faecium AL 41 showed a wide spectrum of inhibitory activity against the indicator organisms from different sources. It was purified by (NH4)2SO4 precipitation, cation-exchange chromatography and reverse phase chromatography (FPLC). The purified peptide ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-007-0226-4

    authors: Mareková M,Lauková A,Skaugen M,Nes I

    更新日期:2007-08-01 00:00:00