Abstract：
:A metabolic network consisting of 48 reactions was established to describe intracellular processes during growth and poly-3-hydroxybutyrate (PHB) production for Cupriavidus necator DSM 545. Glycerol acted as the sole carbon source during exponential, steady-state cultivation conditions. Elementary flux modes were obtained by the program Metatool and analyzed by using yield space analysis. Four sets of elementary modes were obtained, depending on whether the pair NAD/NADH or FAD/FADH2 contributes to the reaction of glycerol-3-phosphate dehydrogenase (GLY-3-P DH), and whether 6-phosphogluconate dehydrogenase (6-PG DH) is present or not. Established metabolic network and the related system of equations provide multiple solutions for the simultaneous synthesis of PHB and biomass; this number of solutions can be further increased if NAD/NADH or FAD/FADH2 were assumed to contribute in the reaction of GLY-3-P DH. As a major outcome, it was demonstrated that experimentally determined yields for biomass and PHB with respect to glycerol fit well to the values obtained in silico when the Entner-Doudoroff pathway (ED) dominates over the glycolytic pathway; this is also the case if the Embden-Meyerhof-Parnas pathway dominates over the ED.
journal_name
J Ind Microbiol Biotechnoljournal_title
Journal of industrial microbiology & biotechnologyauthors
Lopar M,Špoljarić IV,Cepanec N,Koller M,Braunegg G,Horvat Pdoi
10.1007/s10295-014-1439-ysubject
Has Abstractpub_date
2014-06-01 00:00:00pages
913-30issue
6eissn
1367-5435issn
1476-5535journal_volume
41pub_type
杂志文章abstract：:The aspartate pathway of Streptomyces clavuligerus is an important primary metabolic pathway which provides substrates for beta-lactam synthesis. In this study, the hom gene which encodes homoserine dehydrogenase was cloned from the cephamycin C producer S. clavuligerus NRRL 3585 and characterized. The fully sequenced...
journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2008-01-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-010-0729-2
更新日期：2010-08-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2010-02-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2015-01-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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doi：10.1007/s10295-010-0753-2
更新日期：2010-10-01 00:00:00
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更新日期：2011-12-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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doi：10.1007/s10295-009-0590-3
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2001-03-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章,评审
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2005-01-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2014-07-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s10295-007-0234-4
更新日期：2007-09-01 00:00:00
abstract：:Crude glycerol obtained as a by-product of biodiesel production is a reliable feedstock with the potential to be converted into reduced chemicals with high yields. It has been previously shown that ethanol is the primary product of glycerol fermentation by Escherichia coli. However, few efforts were made to enhance th...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2016-10-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s10295-019-02198-y
更新日期：2019-10-01 00:00:00
