Model-based biotechnological potential analysis of Kluyveromyces marxianus central metabolism.

Abstract:

:The non-conventional yeast Kluyveromyces marxianus is an emerging industrial producer for many biotechnological processes. Here, we show the application of a biomass-linked stoichiometric model of central metabolism that is experimentally validated, and mass and charge balanced for assessing the carbon conversion efficiency of wild type and modified K. marxianus. Pairs of substrates (lactose, glucose, inulin, xylose) and products (ethanol, acetate, lactate, glycerol, ethyl acetate, succinate, glutamate, phenylethanol and phenylalanine) are examined by various modelling and optimisation methods. Our model reveals the organism's potential for industrial application and metabolic engineering. Modelling results imply that the aeration regime can be used as a tool to optimise product yield and flux distribution in K. marxianus. Also rebalancing NADH and NADPH utilisation can be used to improve the efficiency of substrate conversion. Xylose is identified as a biotechnologically promising substrate for K. marxianus.

authors

Pentjuss A,Stalidzans E,Liepins J,Kokina A,Martynova J,Zikmanis P,Mozga I,Scherbaka R,Hartman H,Poolman MG,Fell DA,Vigants A

doi

10.1007/s10295-017-1946-8

subject

Has Abstract

pub_date

2017-08-01 00:00:00

pages

1177-1190

issue

8

eissn

1367-5435

issn

1476-5535

pii

10.1007/s10295-017-1946-8

journal_volume

44

pub_type

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