Abstract:
:The non-conventional yeast Kluyveromyces marxianus is an emerging industrial producer for many biotechnological processes. Here, we show the application of a biomass-linked stoichiometric model of central metabolism that is experimentally validated, and mass and charge balanced for assessing the carbon conversion efficiency of wild type and modified K. marxianus. Pairs of substrates (lactose, glucose, inulin, xylose) and products (ethanol, acetate, lactate, glycerol, ethyl acetate, succinate, glutamate, phenylethanol and phenylalanine) are examined by various modelling and optimisation methods. Our model reveals the organism's potential for industrial application and metabolic engineering. Modelling results imply that the aeration regime can be used as a tool to optimise product yield and flux distribution in K. marxianus. Also rebalancing NADH and NADPH utilisation can be used to improve the efficiency of substrate conversion. Xylose is identified as a biotechnologically promising substrate for K. marxianus.
journal_name
J Ind Microbiol Biotechnoljournal_title
Journal of industrial microbiology & biotechnologyauthors
Pentjuss A,Stalidzans E,Liepins J,Kokina A,Martynova J,Zikmanis P,Mozga I,Scherbaka R,Hartman H,Poolman MG,Fell DA,Vigants Adoi
10.1007/s10295-017-1946-8subject
Has Abstractpub_date
2017-08-01 00:00:00pages
1177-1190issue
8eissn
1367-5435issn
1476-5535pii
10.1007/s10295-017-1946-8journal_volume
44pub_type
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