Assessment of physiological state of microorganisms in activated sludge with flow cytometry: application for monitoring sludge production minimization.

Abstract:

:Many sludge reduction processes have been studied for the minimization of sludge production in biological wastewater treatment. The investigations on most of these processes have monitored the increase of the soluble chemical oxygen demand, the sludge mass reduction, or the decrease of the floc size, but little information has been obtained on cell lysis and the change of the biological cell activity. However, employing any strategy for reducing sludge production may have an impact of microbial community in biological wastewater treatment process. This impact may influence the sludge characteristics and the quality of effluent. The objective of this study concerns the determination of the physiological state of activated sludge microorganisms during a sludge minimization process. A thermal treatment at 80 degrees C for 5, 20, 40 and 60 min was chosen in this study. Staining bacteria with CTC and SYTOX green was used to evaluate biological cell activity and viability of cell types contained in activated sludge, respectively. The monitoring of cell activity and viability was performed using flow cytometry (FCM) analysis before and after thermal treatment of activated sludge. Results indicated an increase in the number of permeabilized cells and a decrease in the number of active cells, subsequent to the thermal treatment. The study also confirms the potential of FCM to successfully evaluate the physiological heterogeneity of an activated sludge bacterial population. Moreover, the experimentally observed correlations between the FCM results and the organic matter solubilization in activated sludge samples during thermal treatment revealed that the increase in the soluble organic matter concentration was predominantly due to an intracellular material release. Identifying the increase in activated sludge hydrolysis requires a precise knowledge of the involved mechanisms, and this study indicated that the FCM, used in conjunction with specific probes, could be a useful tool.

authors

Prorot A,Eskicioglu C,Droste R,Dagot C,Leprat P

doi

10.1007/s10295-008-0423-9

subject

Has Abstract

pub_date

2008-11-01 00:00:00

pages

1261-8

issue

11

eissn

1367-5435

issn

1476-5535

journal_volume

35

pub_type

杂志文章
  • Production of bioethanol from organic whey using Kluyveromyces marxianus.

    abstract::Ethanol production by K. marxianus in whey from organic cheese production was examined in batch and continuous mode. The results showed that no pasteurization or freezing of the whey was necessary and that K. marxianus was able to compete with the lactic acid bacteria added during cheese production. The results also s...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-010-0771-0

    authors: Christensen AD,Kádár Z,Oleskowicz-Popiel P,Thomsen MH

    更新日期:2011-02-01 00:00:00

  • Regioselective aromatic hydroxylation of quinaldine by water using quinaldine 4-oxidase in recombinant Pseudomonas putida.

    abstract::Biocatalytic hydrocarbon oxyfunctionalizations are typically accomplished using oxygenases in living bacteria as biocatalysts. These processes are often limited by either oxygen mass transfer, cofactor regeneration, and/or enzyme instabilities due to the formation of reactive oxygen species. Here, we discuss an altern...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-010-0883-6

    authors: Utkür FO,Gaykawad S,Bühler B,Schmid A

    更新日期:2011-08-01 00:00:00

  • Detection and partial characterization of a bacteriocin produced by Carnobacterium piscicola 213.

    abstract::BLIS 213, is a bacteriocin-like inhibitory substance produced by Carnobacterium piscicola 213. It is active against Carnobacterium, Enterococcus and Listeria spp. No activity was observed against tested Lactobacillus, Lactococcus, Leuconostoc and Pediococcus strains, nor against Gram-negative bacteria. The BLIS 213 ac...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1038/sj.jim.2900412

    authors: Khouiti Z,Simon JP

    更新日期:1997-07-01 00:00:00

  • Lasiodiplodan, an exocellular (1→6)-β-D: -glucan from Lasiodiplodia theobromae MMPI: production on glucose, fermentation kinetics, rheology and anti-proliferative activity.

    abstract::Lasiodiplodan, an exopolysaccharide of the (1→6)-β-D: -glucan type, is produced by Lasiodiplodia theobromae MMPI when grown under submerged culture on glucose. The objective of this study was to evaluate lasiodiplodan production by examining the effects of carbon (glucose, fructose, maltose, sucrose) and nitrogen sour...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-012-1112-2

    authors: Alves da Cunha MA,Turmina JA,Ivanov RC,Barroso RR,Marques PT,Fonseca EA,Fortes ZB,Dekker RF,Khaper N,Barbosa AM

    更新日期:2012-08-01 00:00:00

  • Biodegradation of acid blue-15, a textile dye, by an up-flow immobilized cell bioreactor.

    abstract::Acid blue-15, a complex and resonance-stabilized triphenylmethane (TPM) textile dye, resistant to transformation, was decolorized/degraded in an up-flow immobilized cell bioreactor. A consortium comprised of isolates belonging to Bacillus sp., Alcaligenes sp. and Aeromonas sp. formed a multispecies biofilm on refracto...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-004-0121-1

    authors: Sharma DK,Saini HS,Singh M,Chimni SS,Chadha BS

    更新日期:2004-03-01 00:00:00

  • Evolving enzyme technology for pharmaceutical applications: case studies.

    abstract::The case studies focus on two types of enzyme applications for pharmaceutical development. Demethylmacrocin O-methyltransferase, macrocin O-methyltransferase (both putatively rate-limiting) and tylosin reductase were purified from Streptomyces fradiae, characterized and the genes manipulated for increasing tylosin bio...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审

    doi:10.1038/sj.jim.2900437

    authors: Yeh WK

    更新日期:1997-11-01 00:00:00

  • Evaluation of the catalytic specificity, biochemical properties, and milk clotting abilities of an aspartic peptidase from Rhizomucor miehei.

    abstract::In this study, we detail the specificity of an aspartic peptidase from Rhizomucor miehei and evaluate the effects of this peptidase on clotting milk using the peptide sequence of k-casein (Abz-LSFMAIQ-EDDnp) and milk powder. Molecular mass of the peptidase was estimated at 37 kDa, and optimum activity was achieved at ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-016-1780-4

    authors: da Silva RR,Souto TB,de Oliveira TB,de Oliveira LC,Karcher D,Juliano MA,Juliano L,de Oliveira AH,Rodrigues A,Rosa JC,Cabral H

    更新日期:2016-08-01 00:00:00

  • Discovery of a new diol-containing polyketide by heterologous expression of a silent biosynthetic gene cluster from Streptomyces lavendulae FRI-5.

    abstract::The genome of streptomycetes has the ability to produce many novel and potentially useful bioactive compounds, but most of which are not produced under standard laboratory cultivation conditions and are referred to as silent/cryptic secondary metabolites. Streptomyces lavendulae FRI-5 produces several types of bioacti...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-017-1997-x

    authors: Pait IGU,Kitani S,Roslan FW,Ulanova D,Arai M,Ikeda H,Nihira T

    更新日期:2018-02-01 00:00:00

  • Antimicrobial compounds produced by Lactobacillus sakei subsp. sakei 2a, a bacteriocinogenic strain isolated from a Brazilian meat product.

    abstract::Bacteriocins produced by lactic acid bacteria are gaining increased importance due to their activity against undesirable microorganisms in foods. In this study, a concentrated acid extract of a culture of Lactobacillus sakei subsp. sakei 2a, a bacteriocinogenic strain isolated from a Brazilian pork product, was purifi...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-009-0684-y

    authors: de Carvalho KG,Bambirra FH,Kruger MF,Barbosa MS,Oliveira JS,Santos AM,Nicoli JR,Bemquerer MP,de Miranda A,Salvucci EJ,Sesma FJ,Franco BD

    更新日期:2010-04-01 00:00:00

  • A strategy to prevent the occurrence of Lactobacillus strains using lactate-tolerant yeast Candida glabrata in bioethanol production.

    abstract::Contamination of Lactobacillus sp. in the fermentation broth of bioethanol production decreases ethanol production efficiency. Although the addition of lactate to the broth can effectively inhibit the growth of Lactobacillus sp., it also greatly reduces the fermentation ability of Saccharomyces cerevisiae. To overcome...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-008-0390-1

    authors: Watanabe I,Nakamura T,Shima J

    更新日期:2008-10-01 00:00:00

  • Transcriptome analysis of probiotic Lactobacillus casei Zhang during fermentation in soymilk.

    abstract::Lactobacillus casei Zhang is a widely recognized probiotic bacterium, which is being commercially used in China. To study the gene expression dynamics of L. casei Zhang during fermentation in soymilk, a whole genome microarray was used to screen for differentially expressed genes when grown to the lag phase, the late ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-011-1015-7

    authors: Wang JC,Zhang WY,Zhong Z,Wei AB,Bao QH,Zhang Y,Sun TS,Postnikoff A,Meng H,Zhang HP

    更新日期:2012-01-01 00:00:00

  • Production of recombinant herpes simplex virus protease in 10-L stirred vessels using a baculovirus-insect cell expression system.

    abstract::A gene expression system using recombinant Autographa california nuclear polyhedrosis virus (baculovirus) and Sf-9 cells has been scaled up to the 10-L tank level and shown to be capable of producing herpes simplex virus (HSV) protease in serum-free media. High densities of Spodoptera frugiperda (Sf-9) cells were achi...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1038/sj.jim.2900446

    authors: Schwartz JL,Ferrari EB,Terracciano J,Troyanovich J,Gunnarsson I,Wright-Minogue J,Chen JW,Kwong AD

    更新日期:1997-08-01 00:00:00

  • Overcoming inhibitors in a hemicellulosic hydrolysate: improving fermentability by feedstock detoxification and adaptation of Pichia stipitis.

    abstract::In order to improve the fermentative efficiency of sugar maple hemicellulosic hydrolysates for fuel ethanol production, various methods to mitigate the effects of inhibitory compounds were employed. These methods included detoxification treatments utilizing activated charcoal, anion exchange resin, overliming, and eth...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-011-0981-0

    authors: Stoutenburg RM,Perrotta JA,Nakas JP

    更新日期:2011-12-01 00:00:00

  • Enhanced thermostability of keratinase by computational design and empirical mutation.

    abstract::Keratinases are proteolytic enzymes capable of degrading insoluble keratins. The importance of these enzymes is being increasingly recognized in fields as diverse as animal feed production, textile processing, detergent formulation, leather manufacture, and medicine. To enhance the thermostability of Bacillus lichenif...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-013-1268-4

    authors: Liu B,Zhang J,Fang Z,Gu L,Liao X,Du G,Chen J

    更新日期:2013-07-01 00:00:00

  • Degradation of substituted naphthalenesulfonic acids by Sphingomonas xenophaga BN6.

    abstract::Sphingomonas xenophaga BN6 was isolated from the river Elbe as a member of a multispecies bacterial culture which mineralized 6-aminonaphthalene-2-sulfonate. Pure cultures of strain BN6 converted a wide range of amino- and hydroxynaphthalene-2-sulfonates via a catabolic pathway similar to that described for the metabo...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1038/sj.jim.2900725

    authors: Stolz A

    更新日期:1999-10-01 00:00:00

  • Model-based biotechnological potential analysis of Kluyveromyces marxianus central metabolism.

    abstract::The non-conventional yeast Kluyveromyces marxianus is an emerging industrial producer for many biotechnological processes. Here, we show the application of a biomass-linked stoichiometric model of central metabolism that is experimentally validated, and mass and charge balanced for assessing the carbon conversion effi...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-017-1946-8

    authors: Pentjuss A,Stalidzans E,Liepins J,Kokina A,Martynova J,Zikmanis P,Mozga I,Scherbaka R,Hartman H,Poolman MG,Fell DA,Vigants A

    更新日期:2017-08-01 00:00:00

  • Immobilization of Candida rugosa lipase on poly(3-hydroxybutyrate-co-hydroxyvalerate): a new eco-friendly support.

    abstract::The overall objective of this study is to evaluate the morphological [scanning electron microscopy (SEM)], physicochemical [differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), chemical composition analysis, Fourier-transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR)], and bi...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-011-1027-3

    authors: Cabrera-Padilla RY,Lisboa MC,Fricks AT,Franceschi E,Lima AS,Silva DP,Soares CM

    更新日期:2012-02-01 00:00:00

  • Harmful algal blooms: causes, impacts and detection.

    abstract::Blooms of autotrophic algae and some heterotrophic protists are increasingly frequent in coastal waters around the world and are collectively grouped as harmful algal blooms (HABs). Blooms of these organisms are attributed to two primary factors: natural processes such as circulation, upwelling relaxation, and river f...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s10295-003-0074-9

    authors: Sellner KG,Doucette GJ,Kirkpatrick GJ

    更新日期:2003-07-01 00:00:00

  • The performance of fungal xylan-degrading enzyme preparations in elemental chlorine-free bleaching for Eucalyptus pulp.

    abstract::Cellulase-free xylan-degrading enzyme preparations from Acrophialophora nainiana, Humicola grisea var. thermoidea and two Trichoderma harzianum strains were used as bleaching agents for Eucalyptus kraft pulp, prior to a chlorine dioxide and alkaline bleaching sequence. In comparison to the control sequence (performed ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1038/sj/jim/7000227

    authors: Medeiros RG,Silva FG Jr,Salles BC,Estelles RS,Filho EX

    更新日期:2002-04-01 00:00:00

  • Strain improvement for fermentation and biocatalysis processes by genetic engineering technology.

    abstract::Twenty years ago, the first complete gene cluster encoding the actinorhodin biosynthetic pathway was cloned and characterized. Subsequently, the gene clusters encoding the biosynthetic pathways for many antibiotics were isolated. In the past decade, breakthroughs in technology brought that generation of rationally des...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s10295-004-0131-z

    authors: Chiang SJ

    更新日期:2004-03-01 00:00:00

  • Optimising expression of the recombinant fusion protein biopesticide ω-hexatoxin-Hv1a/GNA in Pichia pastoris: sequence modifications and a simple method for the generation of multi-copy strains.

    abstract::Production of recombinant protein bio-insecticides on a commercial scale can only be cost effective if host strains with very high expression levels are available. A recombinant fusion protein containing an arthropod toxin, ω-hexatoxin-Hv1a, (from funnel web spider Hadronyche versuta) linked to snowdrop lectin (Galant...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-014-1466-8

    authors: Pyati P,Fitches E,Gatehouse JA

    更新日期:2014-08-01 00:00:00

  • A microplate spectrofluorometric assay for bacterial biofilms.

    abstract::A spectrofluorometric assay was developed for quantification of bacterial biofilms grown on a microtiter plate. The method involved staining biofilms formed by gram-negative and gram-positive bacteria with wheat germ agglutinin-Alexa Fluor 488 conjugate, which selectively binds to N-acetylglucosamine residues in biofi...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-006-0086-3

    authors: Burton E,Yakandawala N,LoVetri K,Madhyastha MS

    更新日期:2007-01-01 00:00:00

  • In silico aided metabolic engineering of Klebsiella oxytoca and fermentation optimization for enhanced 2,3-butanediol production.

    abstract::Klebsiella oxytoca naturally produces a large amount of 2,3-butanediol (2,3-BD), a promising bulk chemical with wide industrial applications, along with various byproducts. In this study, the in silico gene knockout simulation of K. oxytoca was carried out for 2,3-BD overproduction by inhibiting the formation of bypro...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-013-1298-y

    authors: Park JM,Song H,Lee HJ,Seung D

    更新日期:2013-09-01 00:00:00

  • Optimization of culture conditions for the production of thermostable polygalacturonase by Penicillium SPC-F 20.

    abstract::Penicillium strain isolated from citrus fruit was found to produce thermostable polygalacturonases. Optimization of process parameters resulted in high levels of enzyme production after 3 days of incubation at a pH of 5.0 at 30 degrees C in the presence of 1% pectin. The optimum temperature for enzyme activity was 60 ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-008-0375-0

    authors: Mathew A,Eldo AN,Molly AG

    更新日期:2008-09-01 00:00:00

  • Non-targeted metabolomic reveals the effect of salt stress on global metabolite of halotolerant yeast Candida versatilis and principal component analysis.

    abstract::As one of the major microbes in the soy sauce fermentation, Candida versatilis enriches the flavor and improves the quality of soy sauce. In this study, a combination of five different GC-MS and LC-MS-based metabolome analytical approaches was used to analyze the intracellular, extracellular and whole metabolites of C...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-014-1475-7

    authors: Qi W,Fan ZC,Wang CL,Hou LH,Liu JF,Cao XH

    更新日期:2014-10-01 00:00:00

  • Biotransformation of benzonitrile herbicides via the nitrile hydratase-amidase pathway in rhodococci.

    abstract::The aim of this work was to determine the ability of rhodococci to transform 3,5-dichloro-4-hydroxybenzonitrile (chloroxynil), 3,5-dibromo-4-hydroxybenzonitrile (bromoxynil), 3,5-diiodo-4-hydroxybenzonitrile (ioxynil) and 2,6-dichlorobenzonitrile (dichlobenil); to identify the products and determine their acute toxici...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-012-1184-z

    authors: Veselá AB,Pelantová H,Sulc M,Macková M,Lovecká P,Thimová M,Pasquarelli F,Pičmanová M,Pátek M,Bhalla TC,Martínková L

    更新日期:2012-12-01 00:00:00

  • Novel organization of catechol meta pathway genes in the nitrobenzene degrader Comamonas sp. JS765 and its evolutionary implication.

    abstract::The catechol meta cleavage pathway is one of the central metabolic pathways for the degradation of aromatic compounds. A novel organization of the pathway genes, different from that of classical soil microorganisms, has been observed in Sphingomonas sp HV3 and Pseudomonas sp. DJ77. In a Comamonas sp. JS765, cdoE encod...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-006-0170-8

    authors: He Z,Parales RE,Spain JC,Johnson GR

    更新日期:2007-02-01 00:00:00

  • Insights into the fluoride-resistant regulation mechanism of Acidithiobacillus ferrooxidans ATCC 23270 based on whole genome microarrays.

    abstract::Acidophilic microorganisms involved in uranium bioleaching are usually suppressed by dissolved fluoride ions, eventually leading to reduced leaching efficiency. However, little is known about the regulation mechanisms of microbial resistance to fluoride. In this study, the resistance of Acidithiobacillus ferrooxidans ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-016-1827-6

    authors: Ma L,Li Q,Shen L,Feng X,Xiao Y,Tao J,Liang Y,Yin H,Liu X

    更新日期:2016-10-01 00:00:00

  • Ram horn peptone as a source of citric acid production by Aspergillus niger, with a process.

    abstract::The present study deals with the production of citric acid from a ram horn peptone (RHP) by Aspergillus niger NRRL 330. A medium from RHP and a control medium (CM) were compared for citric acid production using A. niger in a batch culture. For this purpose, first, RHP was produced. Ram horns were hydrolyzed by treatme...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-004-0147-4

    authors: Kurbanoglu EB,Kurbanoglu NI

    更新日期:2004-07-01 00:00:00

  • Bifunctional xylanases and their potential use in biotechnology.

    abstract::Plant cell walls are comprised of cellulose, hemicellulose and other polymers that are intertwined. This complex structure acts as a barrier to degradation by single enzyme. Thus, a cocktail consisting of bi and multifunctional xylanases and xylan debranching enzymes is most desired combination for the efficient utili...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s10295-008-0342-9

    authors: Khandeparker R,Numan MT

    更新日期:2008-07-01 00:00:00