Scalable temperature induced stress for the large-scale production of functionalized Bifidobacteria.


:The application of sub-lethal stresses is known to be an efficient strategy to enhance survival of probiotic bacteria during drying processes. In this context, we previously showed that the application of heat stress upon the entry into stationary phase increased significantly the viability of Bifidobacterium bifidum. However, this heat shock has been considered only in small-scale bioreactor and no information is available for a possible scaling-up strategy. Five different operating scales (0.2, 2, 20, 200 and 2000 L) have thus been tested and the results showed that the viability of B. bifidum increases from 3.15 to 6.57 folds, depending on the scale considered. Our observations pointed out the fact that the heat stress procedure is scalable according to the main outcome, i.e., increases in cell viability, but other factors have to be taken into account. Among these factors, dissolved carbon dioxide seems to play a significant role, since it explains the differences observed between the test performed at laboratory scale and in industrial conditions.


Nguyen HT,Razafindralambo H,Richel A,Jacquet N,Evrard P,Antoine P,Thonart P,Delvigne F




Has Abstract


2015-09-01 00:00:00












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    abstract::L-Methionine is an essential amino acid in humans, which plays an important role in the synthesis of some important amino acids and proteins. In this work, metabolic flux of batch fermentation of L-methionine with recombinant Escherichia coli W3110BL was analyzed using the flux balance analysis method, which estimated...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Niu K,Xu YY,Wu WJ,Zhou HY,Liu ZQ,Zheng YG

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  • Enhancement of rapamycin production by metabolic engineering in Streptomyces hygroscopicus based on genome-scale metabolic model.

    abstract::Rapamycin, as a macrocyclic polyketide with immunosuppressive, antifungal, and anti-tumor activity produced by Streptomyces hygroscopicus, is receiving considerable attention for its significant contribution in medical field. However, the production capacity of the wild strain is very low. Hereby, a computational guid...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Dang L,Liu J,Wang C,Liu H,Wen J

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  • Selection of an autochthonous Saccharomyces strain starter for alcoholic fermentation of Sherry base wines.

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    authors: Rodríguez-Palero MJ,Fierro-Risco J,Codón AC,Benítez T,Valcárcel MJ

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  • Heterotrophic nitrogen removal by Providencia rettgeri strain YL.

    abstract::Providencia rettgeri strain YL was found to be efficient in heterotrophic nitrogen removal under aerobic conditions. Maximum removal of NH(4) (+)-N occurred under the conditions of pH 7 and supplemented with glucose as the carbon source. Inorganic ions such as Mg(2+), Mn(2+), and Zn(2+) largely influenced the growth a...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Zhao B,He YL,Huang J,Taylor S,Hughes J

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  • Genetic manipulation of secondary metabolite biosynthesis for improved production in Streptomyces and other actinomycetes.

    abstract::Actinomycetes continue to be important sources for the discovery of secondary metabolites for applications in human medicine, animal health, and crop protection. With the maturation of actinomycete genome mining as a robust approach to identify new and novel cryptic secondary metabolite gene clusters, it is critical t...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Baltz RH

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 历史文章,杂志文章


    authors: Kyslík P,Prokop A

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  • The effect of albumin fusion structure on the production and bioactivity of the somatostatin-28 fusion protein in Pichia pastoris.

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    journal_title:Journal of industrial microbiology & biotechnology

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    authors: Ding Y,Fan J,Li W,Peng Y,Yang R,Deng L,Fu Q

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  • Construction of advanced producers of first- and second-generation ethanol in Saccharomyces cerevisiae and selected species of non-conventional yeasts (Scheffersomyces stipitis, Ogataea polymorpha).

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Ruchala J,Kurylenko OO,Dmytruk KV,Sibirny AA

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Jiménez-Esquilín AE,Roane TM

    更新日期:2005-08-01 00:00:00

  • Sequential improvement of rimocidin production in Streptomyces rimosus M527 by introduction of cumulative drug-resistance mutations.

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    journal_title:Journal of industrial microbiology & biotechnology

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    authors: Zhao Y,Song Z,Ma Z,Bechthold A,Yu X

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    authors: Moon JW,Ivanov IN,Duty CE,Love LJ,Rondinone AJ,Wang W,Li YL,Madden AS,Mosher JJ,Hu MZ,Suresh AK,Rawn CJ,Jung H,Lauf RJ,Phelps TJ

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Raghuvanshi S,Gupta R

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  • Enhanced poly-γ-L-diaminobutanoic acid production in Bacillus pumilus by combining genome shuffling with multiple antibiotic-resistance.

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Li S,Wang L,Wang N

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  • Synthetic biology, combinatorial biosynthesis, and chemo‑enzymatic synthesis of isoprenoids.

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    pub_type: 杂志文章


    authors: Wang X,Yang H,Ruan L,Liu X,Li F,Xu X

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    authors: D'Souza F,Bruin A,Biersteker R,Donnelly G,Klijnstra J,Rentrop C,Willemsen P

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  • A novel regeneration of iron citrate solution by biooxidation of iron-oxidizing bacteria.

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Wang YJ,Li DP,Liu C,Zhan GQ,He XH

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  • Characterization and comparative analysis of toxin-antitoxin systems in Acetobacter pasteurianus.

    abstract::Bacterial toxin-antitoxin (TA) systems play important roles in diverse cellular regulatory processes. Here, we characterize three putative type II TA candidates from Acetobacter pasteurianus and investigate the profile of type II TA systems in the genus Acetobacter. Based on the gene structure and activity detection, ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Xia K,Bao H,Zhang F,Linhardt RJ,Liang X

    更新日期:2019-06-01 00:00:00

  • Model-based biotechnological potential analysis of Kluyveromyces marxianus central metabolism.

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    authors: Pentjuss A,Stalidzans E,Liepins J,Kokina A,Martynova J,Zikmanis P,Mozga I,Scherbaka R,Hartman H,Poolman MG,Fell DA,Vigants A

    更新日期:2017-08-01 00:00:00

  • Antifungal activity of Bacillus subtilis 355 against wood-surface contaminant fungi.

    abstract::A strain of Bacillus subtilis was examined for antifungal activity against phytopathogenic and wood-surface contaminant fungi. The bacterium was grown in five culture media with different incubation times in order to study cell development, sporulation, and the production of metabolites with antifungal activity. The a...

    journal_title:Journal of industrial microbiology & biotechnology

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    authors: Feio SS,Barbosa A,Cabrita M,Nunes L,Esteves A,Roseiro JC,Curto MJ

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Joshi S,Satyanarayana T

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  • Accumulation of organic acids in cultivations of Neisseria meningitidis C.

    abstract::Aiming at the industrial production of serogroup C meningococcal vaccine, different experimental protocols were tested to cultivate Neisseria meningitidis C and to investigate the related organic acid release. Correlations were established between specific rates of acetic acid and lactic acid accumulation and specific...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Baruque-Ramos J,Hiss H,Converti A,Gonçalves VM,Raw I

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  • Solar Pond devices: free energy or bioreactors for Artemia biomass production?

    abstract::The recent exponential growth in industrial aquaculture has led to a huge increase in Artemia biomass production in order to meet increased fish production needs. The present study explores the potential use of salt gradient solar ponds (SGSPs) for production of Artemia nauplii. An SGSP is a basin of water where solar...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Gouveia L,Sousa J,Marques A,Tavares C,Giestas M

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    abstract::In order to improve the fermentative efficiency of sugar maple hemicellulosic hydrolysates for fuel ethanol production, various methods to mitigate the effects of inhibitory compounds were employed. These methods included detoxification treatments utilizing activated charcoal, anion exchange resin, overliming, and eth...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Stoutenburg RM,Perrotta JA,Nakas JP

    更新日期:2011-12-01 00:00:00

  • Barriers to genome editing with CRISPR in bacteria.

    abstract::Genome editing is essential for probing genotype-phenotype relationships and for enhancing chemical production and phenotypic robustness in industrial bacteria. Currently, the most popular tools for genome editing couple recombineering with DNA cleavage by the CRISPR nuclease Cas9 from Streptococcus pyogenes. Although...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Vento JM,Crook N,Beisel CL

    更新日期:2019-10-01 00:00:00

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Hussain A,Hasnain S

    更新日期:2011-01-01 00:00:00

  • Regioselective aromatic hydroxylation of quinaldine by water using quinaldine 4-oxidase in recombinant Pseudomonas putida.

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Utkür FO,Gaykawad S,Bühler B,Schmid A

    更新日期:2011-08-01 00:00:00

  • Influence of straw types and nitrogen sources on mushroom composting emissions and compost productivity.

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Noble R,Hobbs PJ,Mead A,Dobrovin-Pennington A

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  • Production and single-step purification of Brugia malayi abundant larval transcript (ALT-2) using hydrophobic interaction chromatography.

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Bhuvanesh S,Arunkumar C,Kaliraj P,Ramalingam S

    更新日期:2010-10-01 00:00:00

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Tardif C,Maamar H,Balfin M,Belaich JP

    更新日期:2001-11-01 00:00:00