Abstract：
:The effects of cell immobilization on the secretion of extracellular proteases and glucoamylase production by Aspergillus niger were investigated under a variety of immobilization techniques and culture conditions. Immobilization was achieved by means of cell attachment on metal surfaces or spore entrapment and subsequent growth on porous Celite beads. Free-suspension cultures were compared with immobilized mycelium under culture conditions that included growth in shake flasks and an airlift bioreactor. Cell attachment on metal surfaces minimized the secretion of proteases while enhancing glucoamylase production by the fungus. Growth on Celite beads in shake-flask cultures reduced the specific activity of the secreted proteases from 128 to 61 U g(-1), while glucoamylase specific activity increased from 205 to 350 U g(-1). The effect was more pronounced in bioreactor cultures. A reduction of six orders of magnitude in protease specific activities was observed when the fungus grew immobilized on a rolled metal screen, which served as the draft tube of an airlift bioreactor.
journal_name
J Ind Microbiol Biotechnoljournal_title
Journal of industrial microbiology & biotechnologyauthors
Papagianni M,Joshi N,Moo-Young Mdoi
10.1038/sj.jim.7000289subject
Has Abstractpub_date
2002-11-01 00:00:00pages
259-63issue
5eissn
1367-5435issn
1476-5535pii
7000289journal_volume
29pub_type
杂志文章abstract：:The effect of nutrient supplementation of brewery's spent grain (BSG) hydrolysates was evaluated with respect to biomass and xylitol production by Debaryomyces hansenii. For optimal biomass production, supplementation of full-strength BSG hydrolysates required only phosphate (0.5 g l(-1) KH(2)PO(4)), leading to a biom...
journal_title：Journal of industrial microbiology & biotechnology
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doi：10.1007/s10295-006-0101-8
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journal_title：Journal of industrial microbiology & biotechnology
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doi：10.1007/s10295-013-1395-y
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journal_title：Journal of industrial microbiology & biotechnology
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doi：10.1038/sj.jim.2900681
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1038/sj/jim/7000173
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
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journal_title：Journal of industrial microbiology & biotechnology
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doi：10.1007/s10295-014-1504-6
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2010-09-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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doi：10.1007/s10295-012-1221-y
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-019-02208-z
更新日期：2019-11-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-009-0592-1
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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doi：10.1038/sj.jim.2900725
更新日期：1999-10-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2004-07-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-008-0395-9
更新日期：2008-10-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2012-08-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-009-0585-0
更新日期：2009-08-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-007-0220-x
更新日期：2007-07-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2014-06-01 00:00:00
abstract：:Nucleoside antibiotics constitute an important family of microbial natural products bearing diverse bioactivities and unusual structural features. Their biosynthetic logics are unique with involvement of complex multi-enzymatic reactions leading to the intricate molecules from simple building blocks. Understanding how...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章,评审
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更新日期：2016-03-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-003-0045-1
更新日期：2003-08-01 00:00:00
abstract：:Genetic research enables the evolution of novel biochemical reactions for the production of valuable chemicals from environmentally-friendly raw materials. However, the choice of appropriate microorganisms to support these reactions, which must have strong robustness and be capable of a significant product output, is ...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-017-1956-6
更新日期：2017-08-01 00:00:00
abstract：:Nitrogen deprivation (N-deprivation) is a proven strategy for inducing triacylglyceride accumulation in microalgae. However, its effect on the physical properties of cells and subsequently on product recovery processes is relatively unknown. In this study, the effect of N-deprivation on the cell size, cell wall thickn...
journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2016-12-01 00:00:00
