Process development for production of recombinant human interferon-gamma expressed in Escherichia coli.


:A simple fed-batch process was carried out using constant and variable specific growth rates for high-cell-density cultivation of Escherichia coli BL21 (DE3) expressing human interferon-gamma(hIFN-gamma). The feeding rate was adjusted to achieve an appropriate specific growth rate. The dissolved oxygen level was maintained at 20-30% of air saturation by control of airflow and stirrer speed and, where necessary, by enrichment of inlet air with pure oxygen. Glucose was the sole source of carbon and energy and was provided by following a simple exponential feeding rate. The final cell density in the fed-batch fermentation with constant and variable specific growth rate feeding strategies was ~100 g dry cell wt l(-1) after 36 and 20 h, respectively. The final specific yield and overall productivity of recombinant hIFN-gamma in the variable specific growth rate strategy were 0.35 g rHu-IFN-gamma g(-1) dry cell wt and 0.9 g rHu-IFN-gamma l(-1) h(-1), respectively. A new chromatographic purification procedure involving anion exchange and cation exchange chromatographies was developed for purification of rHu-IFN-gamma from inclusion bodies. The established purification process is reproducible and the total recovery of rHu-IFN-gamma was ~30% (100 mg rHu-IFN-gamma g(-1) dry cell wt). The purity of the rHu-IFN-gamma was determined using HPLC. Sterility, pyrogenicity, and DNA content tests were conducted to assure the absence of toxic materials and other components of E. coli in the final product. The final purified rHu-IFN-gamma has a specific antiviral activity of ~2 x 10(7) IU/mg protein, as determined by viral cytopathic effect assay. These results certify the product for clinical purposes.


Khalilzadeh R,Shojaosadati SA,Maghsoudi N,Mohammadian-Mosaabadi J,Mohammadi MR,Bahrami A,Maleksabet N,Nassiri-Khalilli MA,Ebrahimi M,Naderimanesh H




Has Abstract


2004-02-01 00:00:00












  • On-line biomass measurements in bioreactor cultivations: comparison study of two on-line probes.

    abstract::Two on-line probes for biomass measurement in bioreactor cultivations were evaluated. One probe is based on near infrared (NIR) light absorption and the other on dielectric spectroscopy. The probes were used to monitor biomass production in cultivations of several different microorganisms. Differences in NIR probe res...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Kiviharju K,Salonen K,Moilanen U,Meskanen E,Leisola M,Eerikäinen T

    更新日期:2007-08-01 00:00:00

  • Application of an acid proteinase from Monascus purpureus to reduce antigenicity of bovine milk whey protein.

    abstract::An acid proteinase from Monascus purpureus No. 3403, MpuAP, was previously purified and some characterized in our laboratory (Agric Biol Chem 48:1637-1639, 1984). However, further information about this enzyme is lacking. In this study, we investigated MpuAP's comprehensive substrate specificity, storage stability, an...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Lakshman PL,Tachibana S,Toyama H,Taira T,Suganuma T,Suntornsuk W,Yasuda M

    更新日期:2011-09-01 00:00:00

  • Thermophilic aerobic treatment of a synthetic wastewater in a membrane-coupled bioreactor.

    abstract::Synthetic wastewater containing alpha-lactose and gelatin was treated in a thermophilic membrane-coupled bioreactor (MBR). Thermophilic (>45 degrees C) treatment represents a potentially advantageous process for high-temperature as well as high-strength industrial wastewaters susceptible to reactor autoheating. Thermo...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: LaPara TM,Konopka A,Nakatsu CH,Alleman JE

    更新日期:2001-04-01 00:00:00

  • Informatic search strategies to discover analogues and variants of natural product archetypes.

    abstract::Natural products are a crucial source of antimicrobial agents, but reliance on low-resolution bioactivity-guided approaches has led to diminishing interest in discovery programmes. Here, we demonstrate that two in-house automated informatic platforms can be used to target classes of biologically active natural product...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Johnston CW,Connaty AD,Skinnider MA,Li Y,Grunwald A,Wyatt MA,Kerr RG,Magarvey NA

    更新日期:2016-03-01 00:00:00

  • The impact of Ivan Málek's continuous culture concept on bioprocessing.

    abstract::This paper summarizes research results and their industrial applications obtained by continuous culture in the former Czechoslovakia. Past achievements as well as recent trends and developments worldwide are presented. The term "Prague School of continuous culture" is put forward and its international activity is outl...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 历史文章,杂志文章


    authors: Kyslík P,Prokop A

    更新日期:2010-12-01 00:00:00

  • Natural and engineered biosynthesis of nucleoside antibiotics in Actinomycetes.

    abstract::Nucleoside antibiotics constitute an important family of microbial natural products bearing diverse bioactivities and unusual structural features. Their biosynthetic logics are unique with involvement of complex multi-enzymatic reactions leading to the intricate molecules from simple building blocks. Understanding how...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Chen W,Qi J,Wu P,Wan D,Liu J,Feng X,Deng Z

    更新日期:2016-03-01 00:00:00

  • INDISIM-Denitrification, an individual-based model for study the denitrification process.

    abstract::Denitrification is one of the key processes of the global nitrogen (N) cycle driven by bacteria. It has been widely known for more than 100 years as a process by which the biogeochemical N-cycle is balanced. To study this process, we develop an individual-based model called INDISIM-Denitrification. The model embeds a ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Araujo-Granda P,Gras A,Ginovart M,Moulton V

    更新日期:2020-01-01 00:00:00

  • Kinetic analysis on formation of poly(3-hydroxybutyrate) from acetic acid by Ralstonia eutropha under chemically defined conditions.

    abstract::Batch cultures of Ralstonia eutropha in chemically defined media with acetic acid (HAc) as the sole carbon source were conducted to investigate acetate utilization, formation of poly(3-hydroxybutyrate) (PHB) and growth of active biomass (ABM) under different carbon to nitrogen (C/N) weight ratios. The specific acetate...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Wang J,Yu J

    更新日期:2001-03-01 00:00:00

  • Muscodor albus and its biological promise.

    abstract::We have found a novel fungal genus that produces extremely bioactive volatile organic compounds (VOCs). This fungal isolate was initially discovered as an endophyte in Cinnamomum zeylanicum in a botanical garden in Honduras. This endophytic fungus, Muscodor albus, produces a mixture of VOCs that are lethal to a wide v...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Strobel G

    更新日期:2006-07-01 00:00:00

  • Detection and partial characterization of a bacteriocin produced by Carnobacterium piscicola 213.

    abstract::BLIS 213, is a bacteriocin-like inhibitory substance produced by Carnobacterium piscicola 213. It is active against Carnobacterium, Enterococcus and Listeria spp. No activity was observed against tested Lactobacillus, Lactococcus, Leuconostoc and Pediococcus strains, nor against Gram-negative bacteria. The BLIS 213 ac...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Khouiti Z,Simon JP

    更新日期:1997-07-01 00:00:00

  • Enhancement of Streptomyces transglutaminase activity and pro-peptide cleavage efficiency by introducing linker peptide in the C-terminus of the pro-peptide.

    abstract::Streptomyces transglutaminase (TGase) has been widely used in food, pharmaceutical and textile industries. Streptomyces TGase is naturally synthesized as zymogen (pro-TGase), which is then processed to produce active enzyme by removing its N-terminal pro-peptide. Although the pro-peptide is essential for TGase folding...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Chen K,Liu S,Wang G,Zhang D,Du G,Chen J,Shi Z

    更新日期:2013-04-01 00:00:00

  • Improved production of clavulanic acid by reverse engineering and overexpression of the regulatory genes in an industrial Streptomyces clavuligerus strain.

    abstract::Genomic analysis of the clavulanic acid (CA)-high-producing Streptomyces clavuligerus strains, OL13 and OR, developed through random mutagenesis revealed a frameshift mutation in the cas1 gene-encoding clavaminate synthase 1. Overexpression of the intact cas1 in S. clavuligerus OR enhanced the CA titer by approximatel...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Cho HS,Jo JC,Shin CH,Lee N,Choi JS,Cho BK,Roe JH,Kim CW,Kwon HJ,Yoon YJ

    更新日期:2019-08-01 00:00:00

  • Production of beta-glucosidase using immobilised Piromyces sp. KSX1 and Orpinomyces sp. 478P1 in repeat-batch culture.

    abstract::Two anaerobic fungi, one a monocentric strain ( Piromyces sp. KSX1) and the other a polycentric strain ( Orpinomyces sp. 478P1), were immobilised in calcium alginate beads and cultured in sequential batches where spent medium (containing 0.25% cellobiose) was repeatedly drained and replaced. beta-Glucosidase productio...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: McCabe BK,Kuek C,Gordon GL,Phillips MW

    更新日期:2003-04-01 00:00:00

  • Automated quantification of budding Saccharomyces cerevisiae using a novel image cytometry method.

    abstract::The measurements of concentration, viability, and budding percentages of Saccharomyces cerevisiae are performed on a routine basis in the brewing and biofuel industries. Generation of these parameters is of great importance in a manufacturing setting, where they can aid in the estimation of product quality, quantity, ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Laverty DJ,Kury AL,Kuksin D,Pirani A,Flanagan K,Chan LL

    更新日期:2013-06-01 00:00:00

  • Current approaches to exploit actinomycetes as a source of novel natural products.

    abstract::For decades, microbial natural products have been one of the major sources of novel drugs for pharmaceutical companies, and today all evidence suggests that novel molecules with potential therapeutic applications are still waiting to be discovered from these natural sources, especially from actinomycetes. Any appropri...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Genilloud O,González I,Salazar O,Martín J,Tormo JR,Vicente F

    更新日期:2011-03-01 00:00:00

  • Exploring the diversity and metabolic potential of actinomycetes from temperate marine sediments from Newfoundland, Canada.

    abstract::Marine sediments from Newfoundland, Canada were explored for biotechnologically promising Actinobacteria using culture-independent and culture-dependent approaches. Culture-independent pyrosequencing analyses uncovered significant actinobacterial diversity (H'-2.45 to 3.76), although the taxonomic diversity of biotech...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Duncan KR,Haltli B,Gill KA,Correa H,Berrué F,Kerr RG

    更新日期:2015-01-01 00:00:00

  • Production and single-step purification of Brugia malayi abundant larval transcript (ALT-2) using hydrophobic interaction chromatography.

    abstract::Abundant larval transcript (ALT), a novel filarial protein, has been shown to have great potential as a vaccine in the prevention of human lymphatic filariasis. In this study, we report a method for the production of recombinant ALT-2 protein, expressed in the cytoplasm of bacterium Escherichia coli in soluble form an...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Bhuvanesh S,Arunkumar C,Kaliraj P,Ramalingam S

    更新日期:2010-10-01 00:00:00

  • DNA assembly techniques for next-generation combinatorial biosynthesis of natural products.

    abstract::Natural product scaffolds remain important leads for pharmaceutical development. However, transforming a natural product into a drug entity often requires derivatization to enhance the compound's therapeutic properties. A powerful method by which to perform this derivatization is combinatorial biosynthesis, the manipu...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Cobb RE,Ning JC,Zhao H

    更新日期:2014-02-01 00:00:00

  • Production of recombinant herpes simplex virus protease in 10-L stirred vessels using a baculovirus-insect cell expression system.

    abstract::A gene expression system using recombinant Autographa california nuclear polyhedrosis virus (baculovirus) and Sf-9 cells has been scaled up to the 10-L tank level and shown to be capable of producing herpes simplex virus (HSV) protease in serum-free media. High densities of Spodoptera frugiperda (Sf-9) cells were achi...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Schwartz JL,Ferrari EB,Terracciano J,Troyanovich J,Gunnarsson I,Wright-Minogue J,Chen JW,Kwong AD

    更新日期:1997-08-01 00:00:00

  • Harmful algal blooms: causes, impacts and detection.

    abstract::Blooms of autotrophic algae and some heterotrophic protists are increasingly frequent in coastal waters around the world and are collectively grouped as harmful algal blooms (HABs). Blooms of these organisms are attributed to two primary factors: natural processes such as circulation, upwelling relaxation, and river f...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Sellner KG,Doucette GJ,Kirkpatrick GJ

    更新日期:2003-07-01 00:00:00

  • Methane oxidation by anaerobic archaea for conversion to liquid fuels.

    abstract::Given the recent increases in natural gas reserves and associated drawbacks of current gas-to-liquids technologies, the development of a bioconversion process to directly convert methane to liquid fuels would generate considerable industrial interest. Several clades of anaerobic methanotrophic archaea (ANME) are capab...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Mueller TJ,Grisewood MJ,Nazem-Bokaee H,Gopalakrishnan S,Ferry JG,Wood TK,Maranas CD

    更新日期:2015-03-01 00:00:00

  • Regioselective aromatic hydroxylation of quinaldine by water using quinaldine 4-oxidase in recombinant Pseudomonas putida.

    abstract::Biocatalytic hydrocarbon oxyfunctionalizations are typically accomplished using oxygenases in living bacteria as biocatalysts. These processes are often limited by either oxygen mass transfer, cofactor regeneration, and/or enzyme instabilities due to the formation of reactive oxygen species. Here, we discuss an altern...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Utkür FO,Gaykawad S,Bühler B,Schmid A

    更新日期:2011-08-01 00:00:00

  • Rapidly directional biotransformation of tauroursodeoxycholic acid through engineered Escherichia coli.

    abstract::Bear bile powder is a precious medicinal material. It is characterized by high content of tauroursodeoxycholic acid (TUDCA) at a ratio of 1.0-1.5 to taurochenodeoxycholic acid (TCDCA). Here, we reported the biotransformation of tauroursodeoxycholic acid (TUDCA) through Escherichia coli engineered with a two-step mimic...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Shi J,Wang J,Yu L,Yang L,Zhao S,Wang Z

    更新日期:2017-07-01 00:00:00

  • Isolation and characterization of a new bacteriocin, termed enterocin M, produced by environmental isolate Enterococcus faecium AL41.

    abstract::The new bacteriocin, termed enterocin M, produced by Enterococcus faecium AL 41 showed a wide spectrum of inhibitory activity against the indicator organisms from different sources. It was purified by (NH4)2SO4 precipitation, cation-exchange chromatography and reverse phase chromatography (FPLC). The purified peptide ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Mareková M,Lauková A,Skaugen M,Nes I

    更新日期:2007-08-01 00:00:00

  • Rapid assessment of antimould efficacies of pressure-treated southern pine.

    abstract::A membrane-screening method was developed in conjunction with flow cytometric (FC) analysis for determining the efficacies of antimould pressure-treatment formulations for mould species of cosmetic significance on southern pine. Fusarium subglutinans, Aspergillus flavus, Penicillium chrysogenum, and Paecilomyces spp. ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Price D,Drago G,Noble J,Simmons R,Crow S Jr,Ahearn D

    更新日期:2002-12-01 00:00:00

  • Biodegradation of aromatic compounds under mixed oxygen/denitrifying conditions: a review.

    abstract::Bioremediation of aromatic hydrocarbons in groundwater and sediments is often limited by dissolved oxygen. Many aromatic hydrocarbons degrade very slowly or not at all under anaerobic conditions. Nitrate is a good alternative electron acceptor to oxygen, and denitrifying bacteria are commonly found in the subsurface a...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Wilson LP,Bouwer EJ

    更新日期:1997-02-01 00:00:00

  • A study on the pretreatment of a sugarcane bagasse sample with dilute sulfuric acid.

    abstract::Experiments based on a 2(3) central composite full factorial design were carried out in 200-ml stainless-steel containers to study the pretreatment, with dilute sulfuric acid, of a sugarcane bagasse sample obtained from a local sugar-alcohol mill. The independent variables selected for study were temperature, varied f...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Canilha L,Santos VT,Rocha GJ,Almeida e Silva JB,Giulietti M,Silva SS,Felipe MG,Ferraz A,Milagres AM,Carvalho W

    更新日期:2011-09-01 00:00:00

  • Chemotaxonomic characterisation of Sphingomonas.

    abstract::Based on published results and investigations done for this study, chemotaxonomic characteristics of all validly described species of the genus Sphingomonas, as well as unnamed strains of this genus and related genera such as Rhizomonas and Blastomonas, are presented. All representatives of this group, here designated...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Busse HJ,Kämpfer P,Denner EB

    更新日期:1999-10-01 00:00:00

  • Growth and bacteriocin production by Enterococcus faecium DPC1146 in batch and continuous culture.

    abstract::Production of the bacteriocin enterocin 1146 (E1146) by Enterococcus faecium DPC1146 was studied in batch and continuous fermentation. Growth was strongly inhibited by lactic acid. In batch fermentations maximum E1146 activity (2.8 MBU L-1) was obtained in 9 h with 20 g L-1 glucose. Increase in initial glucose concent...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Parente E,Brienza C,Ricciardi A,Addario G

    更新日期:1997-01-01 00:00:00

  • A novel regeneration of iron citrate solution by biooxidation of iron-oxidizing bacteria.

    abstract::Liquid phase oxidation process using chelated iron solution is among the most promising techniques for the hydrogen sulfide removal due to its double advantage of waste minimization and resource recovery. Regeneration of chelated iron is a core reaction in this process. Regeneration of chelated iron in acidic solution...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Wang YJ,Li DP,Liu C,Zhan GQ,He XH

    更新日期:2014-11-01 00:00:00