Abstract:
:A simple fed-batch process was carried out using constant and variable specific growth rates for high-cell-density cultivation of Escherichia coli BL21 (DE3) expressing human interferon-gamma(hIFN-gamma). The feeding rate was adjusted to achieve an appropriate specific growth rate. The dissolved oxygen level was maintained at 20-30% of air saturation by control of airflow and stirrer speed and, where necessary, by enrichment of inlet air with pure oxygen. Glucose was the sole source of carbon and energy and was provided by following a simple exponential feeding rate. The final cell density in the fed-batch fermentation with constant and variable specific growth rate feeding strategies was ~100 g dry cell wt l(-1) after 36 and 20 h, respectively. The final specific yield and overall productivity of recombinant hIFN-gamma in the variable specific growth rate strategy were 0.35 g rHu-IFN-gamma g(-1) dry cell wt and 0.9 g rHu-IFN-gamma l(-1) h(-1), respectively. A new chromatographic purification procedure involving anion exchange and cation exchange chromatographies was developed for purification of rHu-IFN-gamma from inclusion bodies. The established purification process is reproducible and the total recovery of rHu-IFN-gamma was ~30% (100 mg rHu-IFN-gamma g(-1) dry cell wt). The purity of the rHu-IFN-gamma was determined using HPLC. Sterility, pyrogenicity, and DNA content tests were conducted to assure the absence of toxic materials and other components of E. coli in the final product. The final purified rHu-IFN-gamma has a specific antiviral activity of ~2 x 10(7) IU/mg protein, as determined by viral cytopathic effect assay. These results certify the product for clinical purposes.
journal_name
J Ind Microbiol Biotechnoljournal_title
Journal of industrial microbiology & biotechnologyauthors
Khalilzadeh R,Shojaosadati SA,Maghsoudi N,Mohammadian-Mosaabadi J,Mohammadi MR,Bahrami A,Maleksabet N,Nassiri-Khalilli MA,Ebrahimi M,Naderimanesh Hdoi
10.1007/s10295-004-0117-xsubject
Has Abstractpub_date
2004-02-01 00:00:00pages
63-9issue
2eissn
1367-5435issn
1476-5535journal_volume
31pub_type
杂志文章abstract::We have found a novel fungal genus that produces extremely bioactive volatile organic compounds (VOCs). This fungal isolate was initially discovered as an endophyte in Cinnamomum zeylanicum in a botanical garden in Honduras. This endophytic fungus, Muscodor albus, produces a mixture of VOCs that are lethal to a wide v...
journal_title:Journal of industrial microbiology & biotechnology
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abstract::L-Phenylalanine ammonia-lyase (PAL, EC 4.3.1.25) from Rhodosporidium toruloides was utilized to remove L-phenylalanine (L-Phe) from different commercial protein hydrolysates. A casein acid hydrolysate (CAH, L-Phe ~2.28 %) was employed as a model substrate. t-Cinnamic acid resulting from deamination of L-Phe was extrac...
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abstract::An improved extraction method for ethyl carbamate, a genotoxic and carcinogenic compound found in various fermented foods and beverages, was investigated for its determination in the two most typical Korean traditional rice wines, takju and yakju. When the rice wines were extracted twice with chloroform at 30 degrees ...
journal_title:Journal of industrial microbiology & biotechnology
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abstract::The industrial crops of microalgae use processes calling upon the presence of parts of metal nature such as steel 316L type. The goal of this study is to test the electrochemical behavior of this material in a marine culture of microalgae. Porphyridium purpureum was used under a photoperiod of alternation darkness/lig...
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journal_title:Journal of industrial microbiology & biotechnology
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abstract::Two anaerobic fungi, one a monocentric strain ( Piromyces sp. KSX1) and the other a polycentric strain ( Orpinomyces sp. 478P1), were immobilised in calcium alginate beads and cultured in sequential batches where spent medium (containing 0.25% cellobiose) was repeatedly drained and replaced. beta-Glucosidase productio...
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abstract::The Red recombinase system, the most convenient genetic tool applied in Escherichia coli and other bacteria, was introduced for gene replacement in Klebsiella pneumoniae. The novel K. pneumoniae gene replacement system comprised the Red and FLP recombinases expression vector pDK6-red and pDK6-flp, and linear DNA fragm...
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journal_title:Journal of industrial microbiology & biotechnology
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journal_title:Journal of industrial microbiology & biotechnology
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journal_title:Journal of industrial microbiology & biotechnology
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abstract::Nucleoside antibiotics constitute an important family of microbial natural products bearing diverse bioactivities and unusual structural features. Their biosynthetic logics are unique with involvement of complex multi-enzymatic reactions leading to the intricate molecules from simple building blocks. Understanding how...
journal_title:Journal of industrial microbiology & biotechnology
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journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章,评审
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journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1038/sj/jim/7000173
更新日期:2002-01-01 00:00:00
abstract::Germination of macroconidia and/or microconidia of 24 strains of Fusarium solani, F. chlamydosporum, F. culmorum, F. equiseti, F. verticillioides, F. sambucinum, F. oxysporum and F. proliferatum isolated from fluvial channels and sea beds of the south-eastern coast of Spain, and three control strains (F. oxysporum iso...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
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更新日期:2008-11-01 00:00:00
abstract::The effect of nutrient supplementation of brewery's spent grain (BSG) hydrolysates was evaluated with respect to biomass and xylitol production by Debaryomyces hansenii. For optimal biomass production, supplementation of full-strength BSG hydrolysates required only phosphate (0.5 g l(-1) KH(2)PO(4)), leading to a biom...
journal_title:Journal of industrial microbiology & biotechnology
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journal_title:Journal of industrial microbiology & biotechnology
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journal_title:Journal of industrial microbiology & biotechnology
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abstract::A strain of Bacillus subtilis was examined for antifungal activity against phytopathogenic and wood-surface contaminant fungi. The bacterium was grown in five culture media with different incubation times in order to study cell development, sporulation, and the production of metabolites with antifungal activity. The a...
journal_title:Journal of industrial microbiology & biotechnology
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doi:10.1007/s10295-004-0133-x
更新日期:2004-06-01 00:00:00
abstract::Several indigenous Saccharomyces strains from musts were isolated in the Jerez de la Frontera region, at the end of spontaneous fermentation, in order to select the most suitable autochthonous yeast starter, during the 2007 vintage. Five strains were chosen for their oenological abilities and fermentative kinetics to ...
journal_title:Journal of industrial microbiology & biotechnology
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abstract::The survival of a Sphingomonas species that was introduced into pentachlorophenol (PCP)-contaminated soil was monitored with two complementary methods, a respiration-based assay and a most probable number (MPN) technique. Sphingomonas chlorophenolicastrain RA2 is a PCP-mineralizing bacterium that was introduced into s...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1038/sj.jim.2900718
更新日期:1999-10-01 00:00:00
abstract::Polymetallic ocean nodules offer an alternative source for extracting valuable strategic metals like Cu, Co and Ni. A novel biodissolution process was carried out, employing the cell-free spent growth medium from a marine organism ( Bacillus M1) isolated from nodules; and Cu, Co and Ni solubilization from the nodules ...
journal_title:Journal of industrial microbiology & biotechnology
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abstract::A gene encoding halohydrin dehalogenase (HHDH) from Agrobacterium tumefaciens CCTCC M 87071 was cloned and expressed in Escherichia coli. To increase activity and stability of HHDH, 14 amino acid residues around the active site and substrate-binding pocket based on the structural analysis and molecular docking were se...
journal_title:Journal of industrial microbiology & biotechnology
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journal_title:Journal of industrial microbiology & biotechnology
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更新日期:2004-07-01 00:00:00
abstract::Huperzine A (HupA), a naturally occurring lycopodium alkaloid, is a potent, highly specific and reversible inhibitor of acetylcholinesterase and is a potential treatment for Alzheimer's disease. However, isolating HupA from Huperziaceae plants is inefficient; thus, extracting this compound from endophytic fungi may be...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-014-1461-0
更新日期:2014-07-01 00:00:00
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journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-007-0226-4
更新日期:2007-08-01 00:00:00
abstract::Ethyl carbamate (EC), a pluripotent carcinogen, is mainly formed by a spontaneous chemical reaction of ethanol with urea in wine. The arginine, one of the major amino acids in grape musts, is metabolized by arginase (encoded by CAR1) to ornithine and urea. To reduce the production of urea and EC, an arginase-deficient...
journal_title:Journal of industrial microbiology & biotechnology
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更新日期:2016-05-01 00:00:00
abstract::Bacillus thuringiensis is a Gram-positive bacterium, widely used in agriculture as a biological pesticide. The biocidal activity mainly resides in a parasporal protein inclusion body, or crystal. The inclusion is composed of one or more types of delta-endotoxins (Cry and Cyt proteins). Cry proteins are selectively tox...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1038/sj.jim.2900460
更新日期:1997-09-01 00:00:00