Abstract：
:In the genome of Streptomyces fradiae, the three tylM genes are codirectional with the upstream gene, tylGV. Although the introduction of transcriptional blocks into the tylM genes revealed that they are normally cotranscribed, expression of tylMI still persisted (albeit at a very low level) when either of the upstream genes, tylMII or tylMIII, was disrupted. Such expression apparently resulted from transcriptional initiation at spurious sites that probably contribute insignificantly, if at all, to promote activity in the wild type. Prior to the onset of tylosin production, tylMIII is transcribed independently of tylGV from an authentic promoter buried within tylGV. This latter observation is interesting given that the TGA stop codon of tylGV overlaps the GTG start codon of tylMIII. Evidently, terminally overlapping genes are not always translationally coupled.
journal_name
J Ind Microbiol Biotechnoljournal_title
Journal of industrial microbiology & biotechnologyauthors
Flint SA,Stratigopoulos G,Butler AR,Cundliffe Edoi
10.1038/sj/jim/7000223subject
Has Abstractpub_date
2002-03-01 00:00:00pages
160-7issue
3eissn
1367-5435issn
1476-5535journal_volume
28pub_type
杂志文章abstract：:Twenty years ago, the first complete gene cluster encoding the actinorhodin biosynthetic pathway was cloned and characterized. Subsequently, the gene clusters encoding the biosynthetic pathways for many antibiotics were isolated. In the past decade, breakthroughs in technology brought that generation of rationally des...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章,评审
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abstract：:A formate dehydrogenase gene (fdh) originated from Candida boidinii was co-expressed in E. coli BL21 (DE3) with the cyclohexanone monooxygenase gene (chmo) cloned from Acinetobacter calcoaceticus NCIMB 9871. The co-expression system was then used as a whole-cell biocatalyst to synthesize chiral phenyl methyl sulfoxide...
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journal_title：Journal of industrial microbiology & biotechnology
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doi：10.1007/s10295-004-0121-1
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journal_title：Journal of industrial microbiology & biotechnology
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abstract：:Sphingomonas xenophaga BN6 was isolated from the river Elbe as a member of a multispecies bacterial culture which mineralized 6-aminonaphthalene-2-sulfonate. Pure cultures of strain BN6 converted a wide range of amino- and hydroxynaphthalene-2-sulfonates via a catabolic pathway similar to that described for the metabo...
journal_title：Journal of industrial microbiology & biotechnology
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doi：10.1038/sj.jim.2900725
更新日期：1999-10-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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doi：10.1038/sj.jim.2900681
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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doi：10.1007/s10295-007-0266-9
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journal_title：Journal of industrial microbiology & biotechnology
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doi：10.1038/sj.jim.2900446
更新日期：1997-08-01 00:00:00
abstract：:Whole cultures of Nocardia sp. NRRL 5646 reduce carboxylic acids, first to aldehydes, then to alcohols and subsequently to the corresponding acetyl esters. This work describes an NADPH-dependent reductase responsible for catalyzing the reduction of aldehyde intermediates, which was purified 3240-fold by a combination ...
journal_title：Journal of industrial microbiology & biotechnology
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doi：10.1038/sj.jim.7000096
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abstract：:The application of sub-lethal stresses is known to be an efficient strategy to enhance survival of probiotic bacteria during drying processes. In this context, we previously showed that the application of heat stress upon the entry into stationary phase increased significantly the viability of Bifidobacterium bifidum....
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2010-03-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2012-09-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2014-06-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s10295-009-0683-z
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