Microarray analysis of p-anisaldehyde-induced transcriptome of Saccharomyces cerevisiae.

Abstract:

:p-Anisaldehyde (4-methoxybenzaldehyde), an extract from Pimpinella anisum L. seeds, is a potential novel preservative. To reveal the possible action mechanism of p-anisaldehyde against microorganisms, yeast-based commercial oligonucleotide microarrays were used to analyze the genome-wide transcriptional changes in response to p-anisaldehyde. Quantitative real-time RT-PCR was performed for selected genes to verify the microarray results. We interpreted our microarray data with the clustering tool, T-profiler. Analysis of microarray data revealed that p-anisaldehyde induced the expression of genes related to sulphur assimilation, aromatic aldehydes metabolism, and secondary metabolism, which demonstrated that the addition of p-anisaldehyde may influence the normal metabolism of aromatic aldehydes. This genome-wide transcriptomics approach revealed first insights into the response of Saccharomyces cerevisiae (S. cerevisiae) to p-anisaldehyde challenge.

authors

Yu L,Guo N,Yang Y,Wu X,Meng R,Fan J,Ge F,Wang X,Liu J,Deng X

doi

10.1007/s10295-009-0676-y

subject

Has Abstract

pub_date

2010-03-01 00:00:00

pages

313-22

issue

3

eissn

1367-5435

issn

1476-5535

journal_volume

37

pub_type

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