A very efficient bioconversion of soybean phytosterols mixtures to androstanes by mycobacteria.


:The production of several high value steroid drugs, used as progestational, adrenocortical, estrogenic and contraceptive agents, is mostly derived from 4-androstene-dione (AD) and 1,4 androsta-diene-3,17-dione (ADD). Three Vietnamese phytosterols mixtures named VN-1, VN-2 and VN-3, isolated from soybean oil may be efficiently converted into these key compounds by mycobacterial cells. Their general phytosterol composition was 55.39, 70.55, 70.19% for VN-1, VN-2 and VN-3, respectively. Moreover, values of campesterol, beta-sitosterol and stigmasterol were determined. After 120 h of shaking in suitable culture media and temperature, maximal yield conversion to ADD was higher than 70% and up to 64% to AD, for the various phytosterols mixtures assays. These results may be better when scaling-up such a procedure of phytosterols conversion.


Pérez C,Falero A,Duc HL,Balcinde Y,Hung BR




Has Abstract


2006-08-01 00:00:00












  • Enhanced ethyl caproate production of Chinese liquor yeast by overexpressing EHT1 with deleted FAA1.

    abstract::The fruity odor of Chinese liquor is largely derived from ester formation. Ethyl caproate, an ethyl ester eliciting apple-like flavor, is one of the most important esters in the strong aromatic Chinese liquor (or Luzhou-flavor liquor), which is the most popular and best-selling liquor in China. In the traditional ferm...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Chen Y,Li F,Guo J,Liu G,Guo X,Xiao D

    更新日期:2014-03-01 00:00:00

  • Non-targeted metabolomic reveals the effect of salt stress on global metabolite of halotolerant yeast Candida versatilis and principal component analysis.

    abstract::As one of the major microbes in the soy sauce fermentation, Candida versatilis enriches the flavor and improves the quality of soy sauce. In this study, a combination of five different GC-MS and LC-MS-based metabolome analytical approaches was used to analyze the intracellular, extracellular and whole metabolites of C...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Qi W,Fan ZC,Wang CL,Hou LH,Liu JF,Cao XH

    更新日期:2014-10-01 00:00:00

  • Cell surface hydrophobicity and mycolic acid composition of Rhodococcus strains isolated from activated sludge foam.

    abstract::The bacteria causing foaming in activated sludge plants are considered to be hydrophobic, and their hydrophobicity is assumed to be a crucial factor in their foam-forming ability. This study showed no consistent relationship between cell surface hydrophobicity (CSH), as determined by microbial adherence to hydrocarbon...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Stratton HM,Brooks PR,Griffiths PC,Seviour RJ

    更新日期:2002-05-01 00:00:00

  • Genetic switches and related tools for controlling gene expression and electrical outputs of Geobacter sulfurreducens.

    abstract::Physiological studies and biotechnology applications of Geobacter species have been limited by a lack of genetic tools. Therefore, potential additional molecular strategies for controlling metabolism were explored. When the gene for citrate synthase, or acetyl-CoA transferase, was placed under the control of a LacI/IP...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Ueki T,Nevin KP,Woodard TL,Lovley DR

    更新日期:2016-11-01 00:00:00

  • In vitro colonization of hydrophilic contact lenses by Aspergillus niger.

    abstract::In vitro colonization of hydrophilic contact lenses by Aspergillus niger was investigated. Five strains of the fungus, four polymers, two culture media and four incubation periods were considered for analysis. Only the 2700 strain colonized the lenses. The degrees of adhesion and invasion varied significantly accordin...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Marqués-Calvo MS

    更新日期:2002-07-01 00:00:00

  • Production of beta-glucosidase using immobilised Piromyces sp. KSX1 and Orpinomyces sp. 478P1 in repeat-batch culture.

    abstract::Two anaerobic fungi, one a monocentric strain ( Piromyces sp. KSX1) and the other a polycentric strain ( Orpinomyces sp. 478P1), were immobilised in calcium alginate beads and cultured in sequential batches where spent medium (containing 0.25% cellobiose) was repeatedly drained and replaced. beta-Glucosidase productio...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: McCabe BK,Kuek C,Gordon GL,Phillips MW

    更新日期:2003-04-01 00:00:00

  • Degradation of substituted naphthalenesulfonic acids by Sphingomonas xenophaga BN6.

    abstract::Sphingomonas xenophaga BN6 was isolated from the river Elbe as a member of a multispecies bacterial culture which mineralized 6-aminonaphthalene-2-sulfonate. Pure cultures of strain BN6 converted a wide range of amino- and hydroxynaphthalene-2-sulfonates via a catabolic pathway similar to that described for the metabo...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Stolz A

    更新日期:1999-10-01 00:00:00

  • Effects of acetoacetyl-CoA synthase expression on production of farnesene in Saccharomyces cerevisiae.

    abstract::Efficient production of sesquiterpenes in Saccharomyces cerevisiae requires a high flux through the mevalonate pathway. To achieve this, the supply of acetyl-CoA plays a crucial role, partially because nine moles of acetyl-CoA are necessary to produce one mole of farnesyl diphosphate, but also to overcome the thermody...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Tippmann S,Ferreira R,Siewers V,Nielsen J,Chen Y

    更新日期:2017-06-01 00:00:00

  • Accumulation of organic acids in cultivations of Neisseria meningitidis C.

    abstract::Aiming at the industrial production of serogroup C meningococcal vaccine, different experimental protocols were tested to cultivate Neisseria meningitidis C and to investigate the related organic acid release. Correlations were established between specific rates of acetic acid and lactic acid accumulation and specific...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Baruque-Ramos J,Hiss H,Converti A,Gonçalves VM,Raw I

    更新日期:2006-10-01 00:00:00

  • Evaluation of the catalytic specificity, biochemical properties, and milk clotting abilities of an aspartic peptidase from Rhizomucor miehei.

    abstract::In this study, we detail the specificity of an aspartic peptidase from Rhizomucor miehei and evaluate the effects of this peptidase on clotting milk using the peptide sequence of k-casein (Abz-LSFMAIQ-EDDnp) and milk powder. Molecular mass of the peptidase was estimated at 37 kDa, and optimum activity was achieved at ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: da Silva RR,Souto TB,de Oliveira TB,de Oliveira LC,Karcher D,Juliano MA,Juliano L,de Oliveira AH,Rodrigues A,Rosa JC,Cabral H

    更新日期:2016-08-01 00:00:00

  • Assessing the potential of wild yeasts for bioethanol production.

    abstract::Bioethanol fermentations expose yeasts to a new, complex and challenging fermentation medium with specific inhibitors and sugar mixtures depending on the type of carbon source. It is, therefore, suggested that the natural diversity of yeasts should be further exploited in order to find yeasts with good ethanol yield i...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Ruyters S,Mukherjee V,Verstrepen KJ,Thevelein JM,Willems KA,Lievens B

    更新日期:2015-01-01 00:00:00

  • Synthesis of 4-nitrophenyl acetate using molecular sieve-immobilized lipase from Bacillus coagulans.

    abstract::Extracellular lipase from Bacillus coagulans BTS-3 was immobilized on (3 A x 1.5 mm) molecular sieve. The molecular sieve showed approximately 68.48% binding efficiency for lipase (specific activity 55 IU mg(-1)). The immobilized enzyme achieved approx 90% conversion of acetic acid and 4-nitrophenol (100 mM each) into...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Raghuvanshi S,Gupta R

    更新日期:2009-03-01 00:00:00

  • Optimization of culture conditions for the production of thermostable polygalacturonase by Penicillium SPC-F 20.

    abstract::Penicillium strain isolated from citrus fruit was found to produce thermostable polygalacturonases. Optimization of process parameters resulted in high levels of enzyme production after 3 days of incubation at a pH of 5.0 at 30 degrees C in the presence of 1% pectin. The optimum temperature for enzyme activity was 60 ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Mathew A,Eldo AN,Molly AG

    更新日期:2008-09-01 00:00:00

  • L(+)-lactic acid production by co-fermentation of glucose and xylose with Rhizopus oryzae obtained by low-energy ion beam irradiation.

    abstract::Low-energy ion beam irradiation (10-200 keV) has been proved to have a wide range of biological effects in recent years. When Rhizopus oryzae PW352 was irradiated with a 15-keV low-energy ion beam an L(+)-lactic acid high-yield mutant, RQ4015, was obtained. When 150 g/l glucose was used as the sole carbon source, L(+)...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Wang P,Li J,Wang L,Tang ML,Yu ZL,Zheng ZM

    更新日期:2009-11-01 00:00:00

  • Identification of Geotrichum candidum at the species and strain level: proposal for a standardized protocol.

    abstract::In this study, the M13 primer was used to distinguish Geotrichum candidum from the anamorphic and teleomorphic forms of other arthrospore-forming species (discriminatory power = 0.99). For intraspecific characterization, the GATA4 primer showed the highest level of discrimination for G. candidum among the 20 microsate...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Gente S,Sohier D,Coton E,Duhamel C,Gueguen M

    更新日期:2006-12-01 00:00:00

  • Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach.

    abstract::Diesel fuel is one of the most important sources of hydrocarbon contamination worldwide. Its composition consists of a complex mixture of n-alkanes, branched alkanes and aromatic compounds. Hydrocarbon degradation in Pseudomonas species has been mostly studied under aerobic conditions; however, a dynamic spectrum of o...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Tribelli PM,Rossi L,Ricardi MM,Gomez-Lozano M,Molin S,Raiger Iustman LJ,Lopez NI

    更新日期:2018-01-01 00:00:00

  • Rapid assessment of antimould efficacies of pressure-treated southern pine.

    abstract::A membrane-screening method was developed in conjunction with flow cytometric (FC) analysis for determining the efficacies of antimould pressure-treatment formulations for mould species of cosmetic significance on southern pine. Fusarium subglutinans, Aspergillus flavus, Penicillium chrysogenum, and Paecilomyces spp. ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Price D,Drago G,Noble J,Simmons R,Crow S Jr,Ahearn D

    更新日期:2002-12-01 00:00:00

  • An overview of rapamycin: from discovery to future perspectives.

    abstract::Rapamycin is an immunosuppressive metabolite produced from several actinomycete species. Besides its immunosuppressive activity, rapamycin and its analogs have additional therapeutic potentials, including antifungal, antitumor, neuroprotective/neuroregenerative, and lifespan extension activities. The core structure of...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Yoo YJ,Kim H,Park SR,Yoon YJ

    更新日期:2017-05-01 00:00:00

  • Assessment of physiological state of microorganisms in activated sludge with flow cytometry: application for monitoring sludge production minimization.

    abstract::Many sludge reduction processes have been studied for the minimization of sludge production in biological wastewater treatment. The investigations on most of these processes have monitored the increase of the soluble chemical oxygen demand, the sludge mass reduction, or the decrease of the floc size, but little inform...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Prorot A,Eskicioglu C,Droste R,Dagot C,Leprat P

    更新日期:2008-11-01 00:00:00

  • Detection of bacterial contamination in starch and resin-based papermaking chemicals using fluorescence techniques.

    abstract::Rapid fluorescence techniques were evaluated for the detection of bacterial contaminants in papermaking chemicals including starch and the resin-based sizes and starch slurries used in the paper industry. Viable and non-viable bacterial cells were visualised by fluorescent probes and detected by epifluorescence micros...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Nohynek L,Saski E,Haikara A,Raaska L

    更新日期:2003-04-01 00:00:00

  • Isolation and characterization of a biosurfactant producing strain, Brevibacilis brevis HOB1.

    abstract::Biosurfactant-producing bacteria were isolated from the production water of an oil field. Isolates were screened for biosurfactant production using surface tension test. The highest reduction of surface tension was achieved with a bacterial strain which was identified by 16S rRNA gene sequencing as Brevibacilis brevis...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Haddad NI,Wang J,Mu B

    更新日期:2008-12-01 00:00:00

  • Five gene products are required for assembly of the central pyrrole moiety of coumermycin A1.

    abstract::Coumermycin A1 is an aminocoumarin antibiotic produced by Streptomyces rishiriensis. It exhibits potent antibacterial and anticancer activity. The coumermycin A1 molecule contains two terminal 5-methyl-pyrrole-2-carboxylic acid moieties and one central 3-methylpyrrole-2,4-dicarboxylic acid moiety (CPM). While the bios...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Novotna J,Gust B,Kulik A,Spizek J,Heide L

    更新日期:2013-08-01 00:00:00

  • The impact of Ivan Málek's continuous culture concept on bioprocessing.

    abstract::This paper summarizes research results and their industrial applications obtained by continuous culture in the former Czechoslovakia. Past achievements as well as recent trends and developments worldwide are presented. The term "Prague School of continuous culture" is put forward and its international activity is outl...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 历史文章,杂志文章


    authors: Kyslík P,Prokop A

    更新日期:2010-12-01 00:00:00

  • Evolving enzyme technology for pharmaceutical applications: case studies.

    abstract::The case studies focus on two types of enzyme applications for pharmaceutical development. Demethylmacrocin O-methyltransferase, macrocin O-methyltransferase (both putatively rate-limiting) and tylosin reductase were purified from Streptomyces fradiae, characterized and the genes manipulated for increasing tylosin bio...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Yeh WK

    更新日期:1997-11-01 00:00:00

  • Targeted disruption of homoserine dehydrogenase gene and its effect on cephamycin C production in Streptomyces clavuligerus.

    abstract::The aspartate pathway of Streptomyces clavuligerus is an important primary metabolic pathway which provides substrates for beta-lactam synthesis. In this study, the hom gene which encodes homoserine dehydrogenase was cloned from the cephamycin C producer S. clavuligerus NRRL 3585 and characterized. The fully sequenced...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Yilmaz EI,Caydasi AK,Ozcengiz G

    更新日期:2008-01-01 00:00:00

  • Identification and characterization of a novel cold-adapted esterase from a metagenomic library of mountain soil.

    abstract::A novel lipolytic enzyme was isolated from a metagenomic library after demonstration of lipolytic activity on an LB agar plate containing 1% (w/v) tributyrin. A novel esterase gene (estIM1), encoding a lipolytic enzyme (EstIM1), was cloned using a shotgun method from a pFosEstIM1 clone of the metagenomic library, and ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Ko KC,Rim SO,Han Y,Shin BS,Kim GJ,Choi JH,Song JJ

    更新日期:2012-05-01 00:00:00

  • A simulation study comparing the impact of experimental error on the performance of experimental designs and artificial neural networks used for process screening.

    abstract::Many variables and their interactions can affect a biotechnological process. Testing a large number of variables and all their possible interactions is a cumbersome task and its cost can be prohibitive. Several screening strategies, with a relatively low number of experiments, can be used to find which variables have ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Soria MA,Gonzalez Funes JL,Garcia AF

    更新日期:2004-11-01 00:00:00

  • Rapid and stable production of 2,3-butanediol by an engineered Saccharomyces cerevisiae strain in a continuous airlift bioreactor.

    abstract::Utilization of renewable feedstocks for the production of bio-based bulk chemicals, such as 2,3-butanediol (2,3-BDO), by engineered strains of the non-pathogenic yeast, Saccharomyces cerevisiae, has recently become an attractive option. In this study, to realize rapid production of 2,3-BDO, a flocculent, 2,3-BDO-produ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Yamada R,Nishikawa R,Wakita K,Ogino H

    更新日期:2018-05-01 00:00:00

  • Improvement of bioremediation by Pseudomonas and Burkholderia by mutants of the Vitreoscilla hemoglobin gene (vgb) integrated into their chromosomes.

    abstract::Using genetic engineering, the Vitreoscilla (bacterial) hemoglobin gene (vgb) was integrated stably into the chromosomes of Pseudomonas aeruginosa and Burkholderia sp. strain DNT. This was done for both wild type vgb and two site-directed mutants of vgb that produce Vitreoscilla hemoglobin (VHb) with lowered oxygen af...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Kim Y,Webster DA,Stark BC

    更新日期:2005-04-01 00:00:00

  • Microarray analysis of p-anisaldehyde-induced transcriptome of Saccharomyces cerevisiae.

    abstract::p-Anisaldehyde (4-methoxybenzaldehyde), an extract from Pimpinella anisum L. seeds, is a potential novel preservative. To reveal the possible action mechanism of p-anisaldehyde against microorganisms, yeast-based commercial oligonucleotide microarrays were used to analyze the genome-wide transcriptional changes in res...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Yu L,Guo N,Yang Y,Wu X,Meng R,Fan J,Ge F,Wang X,Liu J,Deng X

    更新日期:2010-03-01 00:00:00