Engineering Yarrowia lipolytica for the production of cyclopropanated fatty acids.


:Traditional synthesis of biodiesel competes with food sources and has limitations with storage, particularly due to limited oxidative stability. Microbial synthesis of lipids provides a platform to produce renewable fuel with improved properties from various renewable carbon sources. Specifically, biodiesel properties can be improved through the introduction of a cyclopropane ring in place of a double bond. In this study, we demonstrate the production of C19 cyclopropanated fatty acids in the oleaginous yeast Yarrowia lipolytica through the heterologous expression of the Escherichia coli cyclopropane fatty acid synthase. Ultimately, we establish a strain capable of 3.03 ± 0.26 g/L C19 cyclopropanated fatty acid production in bioreactor fermentation where this functionalized lipid comprises over 32% of the total lipid pool. This study provides a demonstration of the flexibility of lipid metabolism in Y. lipolytica to produce specialized fatty acids.


Markham KA,Alper HS




Has Abstract


2018-10-01 00:00:00














  • Advances in microbial culturing conditions to activate silent biosynthetic gene clusters for novel metabolite production.

    abstract::Natural products (NPs) produced by bacteria and fungi are often used as therapeutic agents due to their complex structures and wide range of bioactivities. Enzymes that build NPs are encoded by co-localized biosynthetic gene clusters (BGCs), and genome sequencing has recently revealed that many BGCs are "silent" under...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Tomm HA,Ucciferri L,Ross AC

    更新日期:2019-10-01 00:00:00

  • Enhancement of maltose utilisation by Saccharomyces cerevisiae in medium containing fermentable hexoses.

    abstract::Some industrial strains of Saccharomyces cerevisiae are unable to maintain high rates of fermentation during transition from catabolism of hexoses to maltose. This phenomenon, termed 'maltose lag', presents problems for the baking, brewing and distilling industries, which rely on yeast catabolism of mixtures of hexose...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Hazell B,Attfield P

    更新日期:1999-06-01 00:00:00

  • A microplate spectrofluorometric assay for bacterial biofilms.

    abstract::A spectrofluorometric assay was developed for quantification of bacterial biofilms grown on a microtiter plate. The method involved staining biofilms formed by gram-negative and gram-positive bacteria with wheat germ agglutinin-Alexa Fluor 488 conjugate, which selectively binds to N-acetylglucosamine residues in biofi...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Burton E,Yakandawala N,LoVetri K,Madhyastha MS

    更新日期:2007-01-01 00:00:00

  • Start-up and inhibition analysis of the Anammox process seeded with anaerobic granular sludge.

    abstract::The longer start-up period of the Anammox process is due to the very low cellular yield and growth rates of Anammox bacteria. Nitrite inhibition is considered to be the key factor in the instability of the Anammox process during the operation. However, little attention was paid to the inhibitory effect of pH and free ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Tang CJ,Zheng P,Mahmood Q,Chen JW

    更新日期:2009-08-01 00:00:00

  • Detection of bacterial contamination in starch and resin-based papermaking chemicals using fluorescence techniques.

    abstract::Rapid fluorescence techniques were evaluated for the detection of bacterial contaminants in papermaking chemicals including starch and the resin-based sizes and starch slurries used in the paper industry. Viable and non-viable bacterial cells were visualised by fluorescent probes and detected by epifluorescence micros...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Nohynek L,Saski E,Haikara A,Raaska L

    更新日期:2003-04-01 00:00:00

  • Natural and engineered biosynthesis of nucleoside antibiotics in Actinomycetes.

    abstract::Nucleoside antibiotics constitute an important family of microbial natural products bearing diverse bioactivities and unusual structural features. Their biosynthetic logics are unique with involvement of complex multi-enzymatic reactions leading to the intricate molecules from simple building blocks. Understanding how...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Chen W,Qi J,Wu P,Wan D,Liu J,Feng X,Deng Z

    更新日期:2016-03-01 00:00:00

  • Strain development of Streptomyces sp. for tacrolimus production using sequential adaptation.

    abstract::An immunosuppressant tacrolimus-producing strain of Streptomyces sp. TST8 was isolated and developed by the TS corporation in Korea using the sequential adaptation of media containing tacrolimus (600-1600 mg/l). The aim of the tacrolimus sequential adaptation protocol was to select those cells with tacrolimus resistan...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Jung S,Moon S,Lee K,Park YJ,Yoon S,Yoo YJ

    更新日期:2009-12-01 00:00:00

  • Rapid and stable production of 2,3-butanediol by an engineered Saccharomyces cerevisiae strain in a continuous airlift bioreactor.

    abstract::Utilization of renewable feedstocks for the production of bio-based bulk chemicals, such as 2,3-butanediol (2,3-BDO), by engineered strains of the non-pathogenic yeast, Saccharomyces cerevisiae, has recently become an attractive option. In this study, to realize rapid production of 2,3-BDO, a flocculent, 2,3-BDO-produ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Yamada R,Nishikawa R,Wakita K,Ogino H

    更新日期:2018-05-01 00:00:00

  • Analysis of respiratory activity and carbon usage of a mutant of Azotobacter vinelandii impaired in poly-β-hydroxybutyrate synthesis.

    abstract::In this study, the respiratory activity and carbon usage of the mutant strain of A. vinelandii AT6, impaired in poly-β-hydroxybutyrate (PHB) production, and their relationship with the synthesis of alginate were evaluated. The alginate yield and the specific oxygen uptake rate were higher (2.5-fold and 62 %, respectiv...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Jiménez L,Castillo T,Flores C,Segura D,Galindo E,Peña C

    更新日期:2016-08-01 00:00:00

  • Heterologous production of a new lasso peptide brevunsin in Sphingomonas subterranea.

    abstract::A shuttle vector pHSG396Sp was constructed to perform gene expression using Sphingomonas subterranea as a host. A new lasso peptide biosynthetic gene cluster, derived from Brevundimonas diminuta, was amplified by PCR and integrated to afford a expression vector pHSG396Sp-12697L. The new lasso peptide brevunsin was suc...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Kodani S,Hemmi H,Miyake Y,Kaweewan I,Nakagawa H

    更新日期:2018-11-01 00:00:00

  • A novel high-throughput and quantitative method based on visible color shifts for screening Bacillus subtilis THY-15 for surfactin production.

    abstract::A novel chromatic visible screening method using bromothymol blue (BTB) as a color indicator and cetylpyridinium chloride (CPC) as a mediator was constructed to obtain the high titer surfactin-producing strains. The reliability and quantification accuracy of color shift were also confirmed. Regular chromatic responses...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Yang H,Yu H,Shen Z

    更新日期:2015-08-01 00:00:00

  • Evaluation of liquid and solid culture media for the recovery and enrichment of Burkholderia cenocepacia from distilled water.

    abstract::Burkholderia cepacia complex (BCC) presence has been the cause of recalls of both sterile and non-sterile pharmaceutical products since these opportunistic pathogens have been implicated to cause infections to susceptible individuals. BCC are ubiquitous in nature, but in pharmaceutical settings the most common source ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Ahn Y,Kim JM,Ahn H,Lee YJ,LiPuma JJ,Hussong D,Cerniglia CE

    更新日期:2014-07-01 00:00:00

  • Comparative analysis of oxygen transfer rate distribution in stirred bioreactor for simulated and real fermentation broths.

    abstract::Study of the distribution of the oxygen mass transfer coefficient, k (l) a, for a stirred bioreactor and simulated (pseudoplastic solutions of carboxymethylcellulose sodium salt) bacterial (P. shermanii), yeast (S. cerevisiae), and fungal (P. chrysogenum free mycelia) broths indicated significant variation of transfer...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Caşcaval D,Galaction AI,Turnea M

    更新日期:2011-09-01 00:00:00

  • Characterization and comparative analysis of toxin-antitoxin systems in Acetobacter pasteurianus.

    abstract::Bacterial toxin-antitoxin (TA) systems play important roles in diverse cellular regulatory processes. Here, we characterize three putative type II TA candidates from Acetobacter pasteurianus and investigate the profile of type II TA systems in the genus Acetobacter. Based on the gene structure and activity detection, ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Xia K,Bao H,Zhang F,Linhardt RJ,Liang X

    更新日期:2019-06-01 00:00:00

  • Supplementation requirements of brewery's spent grain hydrolysate for biomass and xylitol production by Debaryomyces hansenii CCMI 941.

    abstract::The effect of nutrient supplementation of brewery's spent grain (BSG) hydrolysates was evaluated with respect to biomass and xylitol production by Debaryomyces hansenii. For optimal biomass production, supplementation of full-strength BSG hydrolysates required only phosphate (0.5 g l(-1) KH(2)PO(4)), leading to a biom...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Carvalheiro F,Duarte LC,Lopes S,Parajó JC,Pereira H,Gírio FM

    更新日期:2006-08-01 00:00:00

  • Influence of straw types and nitrogen sources on mushroom composting emissions and compost productivity.

    abstract::The effects of different straw types and organic and inorganic nitrogen (N) sources on the chemical composition and odor concentration (OC) of mushroom composting emissions, compost parameters, and mushroom yield were examined using bench-scale and large-scale (windrows and aerated tunnels) composting systems. There w...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Noble R,Hobbs PJ,Mead A,Dobrovin-Pennington A

    更新日期:2002-09-01 00:00:00

  • Bacterial cell attachment, the beginning of a biofilm.

    abstract::The ability of bacteria to attach to surfaces and develop into a biofilm has been of considerable interest to many groups in numerous industries, including the medical and food industry. However, little is understood in the critical initial step seen in all biofilm development, the initial bacterial cell attachment to...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Palmer J,Flint S,Brooks J

    更新日期:2007-09-01 00:00:00

  • Cloning and characterization of a thermostable superoxide dismutase from the thermophilic bacterium Rhodothermus sp. XMH10.

    abstract::A superoxide dismutase (SOD) gene was cloned from the thermophilic bacterium Rhodothermus sp. XMH10 for the first time and highly expressed in Escherichia coli. The Rhodothermus sp. XMH10 SOD (RhSOD) gene encodes 209 amino acids with a putative molecular weight of 23.6 kDa and a pI value of 5.53. The recombinant RhSOD...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Wang X,Yang H,Ruan L,Liu X,Li F,Xu X

    更新日期:2008-02-01 00:00:00

  • Use of (1)H NMR to study transport processes in porous biosystems.

    abstract::The operation of bioreactors and the metabolism of microorganisms in biofilms or soil/sediment systems are strongly dictated by the transport processes therein. Nuclear magnetic resonance (NMR) spectroscopy or magnetic resonance imaging (MRI) allow nondestructive and noninvasive quantification and visualisation (in ca...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Van As H,Lens P

    更新日期:2001-01-01 00:00:00

  • Biological deinking of inkjet-printed paper using Vibrio alginolyticus and its enzymes.

    abstract::Recycling of office waste paper (photocopy, inkjet, and laser prints) is a major problem due to difficulty in removal of nonimpact ink. Biological deinking of office waste paper is reported using several microorganisms and their enzymes. We report here deinking and decolorization of the dislodged ink particles from in...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Mohandass C,Raghukumar C

    更新日期:2005-09-01 00:00:00

  • Biodegradation of acid blue-15, a textile dye, by an up-flow immobilized cell bioreactor.

    abstract::Acid blue-15, a complex and resonance-stabilized triphenylmethane (TPM) textile dye, resistant to transformation, was decolorized/degraded in an up-flow immobilized cell bioreactor. A consortium comprised of isolates belonging to Bacillus sp., Alcaligenes sp. and Aeromonas sp. formed a multispecies biofilm on refracto...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Sharma DK,Saini HS,Singh M,Chimni SS,Chadha BS

    更新日期:2004-03-01 00:00:00

  • Mechanisms for solubilization of cobalt, copper and nickel from Indian Ocean nodules at near neutral pH by a marine isolate.

    abstract::Polymetallic ocean nodules offer an alternative source for extracting valuable strategic metals like Cu, Co and Ni. A novel biodissolution process was carried out, employing the cell-free spent growth medium from a marine organism ( Bacillus M1) isolated from nodules; and Cu, Co and Ni solubilization from the nodules ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Mukherjee A,Raichur AM,Modak JM,Natarajan KA

    更新日期:2004-11-01 00:00:00

  • Identification of oleaginous yeasts that metabolize aromatic compounds.

    abstract::The valorization of lignin is critical for the economic viability of the bioeconomy. Microbial metabolism is advantageous for handling the myriad of aromatic compounds resulting from lignin chemical or enzymatic depolymerization. Coupling aromatic metabolism to fatty acid biosynthesis makes possible the production of ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Yaguchi A,Franaszek N,O'Neill K,Lee S,Sitepu I,Boundy-Mills K,Blenner M

    更新日期:2020-10-01 00:00:00

  • Ammonia oxidizing bacteria and archaea in horizontal flow biofilm reactors treating ammonia-contaminated air at 10 °C.

    abstract::The objective of this study was to demonstrate the feasibility of novel, Horizontal Flow Biofilm Reactor (HFBR) technology for the treatment of ammonia (NH3)-contaminated airstreams. Three laboratory-scale HFBRs were used for remediation of an NH3-containing airstream at 10 °C during a 90-d trial to test the efficacy ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Gerrity S,Clifford E,Kennelly C,Collins G

    更新日期:2016-05-01 00:00:00

  • An inexpensive medium for production of arachidonic acid by Mortierella alpina.

    abstract::The production of arachidonic acid was studied in the fungus Mortierella alpina using an inexpensive medium. Glucose derived from maize starch hydrolysate was the sole carbon source and defatted soybean meal and sodium nitrate were the nitrogen sources. Optimal arachidonic acid yield (1.47 g l(-1)) was observed at a g...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Zhu M,Yu LJ,Wu YX

    更新日期:2003-01-01 00:00:00

  • Exploring the diversity and metabolic potential of actinomycetes from temperate marine sediments from Newfoundland, Canada.

    abstract::Marine sediments from Newfoundland, Canada were explored for biotechnologically promising Actinobacteria using culture-independent and culture-dependent approaches. Culture-independent pyrosequencing analyses uncovered significant actinobacterial diversity (H'-2.45 to 3.76), although the taxonomic diversity of biotech...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Duncan KR,Haltli B,Gill KA,Correa H,Berrué F,Kerr RG

    更新日期:2015-01-01 00:00:00

  • Corrections to: Enhanced lincomycin production by co‑overexpression of metK1 and metK2 in Streptomyces lincolnensis.

    abstract::In the online published article, row value "pIB139-metK1-metK2" in table 1 has been processed incorrectly. The correct table is given below. ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,已发布勘误


    authors: Xu Y,Tan G,Ke M,Li J,Tang Y,Meng S,Niu J,Wang Y,Liu R,Wu H,Bai L,Zhang L,Zhang B

    更新日期:2018-06-01 00:00:00

  • Functional expression of a single-chain antibody fragment against human epidermal growth factor receptor 2 (HER2) in Escherichia coli.

    abstract::The human epidermal growth factor receptor (HER) family plays an important role in cell growth and signaling and alteration of its function has been demonstrated in many different kinds of cancer. Receptor dimerization is necessary for the HER signal transduction pathway and tyrosine kinase activity. Recently, several...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Akbari V,Mir Mohammad Sadeghi H,Jafrian-Dehkordi A,Abedi D,Chou CP

    更新日期:2014-06-01 00:00:00

  • Strain improvement for fermentation and biocatalysis processes by genetic engineering technology.

    abstract::Twenty years ago, the first complete gene cluster encoding the actinorhodin biosynthetic pathway was cloned and characterized. Subsequently, the gene clusters encoding the biosynthetic pathways for many antibiotics were isolated. In the past decade, breakthroughs in technology brought that generation of rationally des...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Chiang SJ

    更新日期:2004-03-01 00:00:00

  • Evolving enzyme technology for pharmaceutical applications: case studies.

    abstract::The case studies focus on two types of enzyme applications for pharmaceutical development. Demethylmacrocin O-methyltransferase, macrocin O-methyltransferase (both putatively rate-limiting) and tylosin reductase were purified from Streptomyces fradiae, characterized and the genes manipulated for increasing tylosin bio...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Yeh WK

    更新日期:1997-11-01 00:00:00