Engineering Yarrowia lipolytica for the production of cyclopropanated fatty acids.

Abstract:

:Traditional synthesis of biodiesel competes with food sources and has limitations with storage, particularly due to limited oxidative stability. Microbial synthesis of lipids provides a platform to produce renewable fuel with improved properties from various renewable carbon sources. Specifically, biodiesel properties can be improved through the introduction of a cyclopropane ring in place of a double bond. In this study, we demonstrate the production of C19 cyclopropanated fatty acids in the oleaginous yeast Yarrowia lipolytica through the heterologous expression of the Escherichia coli cyclopropane fatty acid synthase. Ultimately, we establish a strain capable of 3.03 ± 0.26 g/L C19 cyclopropanated fatty acid production in bioreactor fermentation where this functionalized lipid comprises over 32% of the total lipid pool. This study provides a demonstration of the flexibility of lipid metabolism in Y. lipolytica to produce specialized fatty acids.

authors

Markham KA,Alper HS

doi

10.1007/s10295-018-2067-8

subject

Has Abstract

pub_date

2018-10-01 00:00:00

pages

881-888

issue

10

eissn

1367-5435

issn

1476-5535

pii

10.1007/s10295-018-2067-8

journal_volume

45

pub_type

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