Abstract：
:A halotolerant isolate Bacillus sp. L1 producing extracellular cellulase was isolated from Yuncheng, China. Production of the enzyme started from mid-exponential phase of bacterial growth and reached a maximum level during the post-stationary phase. The cellulase was purified to homogeneity with molecular mass of 45 kDa. Substrate specificity test indicated that it was an endoglucanase for soluble cellulose. Optimal enzyme activity was found to be at 60 °C, pH 8.0, and 7.5 % NaCl. Furthermore, it was highly active and stable over broad ranges of temperature (30-80 °C), pH (7.0-9.0), and NaCl concentration (2.5-15 %), thus showing its excellent thermostable, alkali-stable, and halotolerant nature. The cellulase activity was greatly inhibited by ethylenediaminetetraacetic acid, indicating that it was a metalloenzyme. Significant inhibition by phenylmethylsulfonyl fluoride and phenylarsine oxide revealed that serine and cysteine residues were essential for the enzyme catalysis. Moreover, the cellulase was highly active in the presence of surfactants, and it showed high stability in the presence of water-insoluble organic solvents with log P (ow)at least 0.88. Results from this study indicate that the purified cellulase from isolate L1 may have considerable potential for industrial application owing to its useful properties.
journal_name
J Ind Microbiol Biotechnoljournal_title
Journal of industrial microbiology & biotechnologyauthors
Li X,Yu HYdoi
10.1007/s10295-012-1120-2subject
Has Abstractpub_date
2012-08-01 00:00:00pages
1117-24issue
8eissn
1367-5435issn
1476-5535journal_volume
39pub_type
杂志文章abstract：:Robust microorganisms are necessary for economical bioethanol production. However, such organisms must be able to effectively ferment both hexose and pentose sugars present in lignocellulosic hydrolysate to ethanol. Wild type Saccharomyces cerevisiae can rapidly ferment hexose, but cannot ferment pentose sugars. Consi...
journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2008-04-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2011-06-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2010-03-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2015-01-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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doi：10.1007/s10295-014-1437-0
更新日期：2014-06-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2000-12-01 00:00:00
abstract：:There have been numerous developments in ethanol fermentation technology since the beginning of the new millennium as ethanol has become an immediate viable alternative to fast-depleting crude reserves as well as increasing concerns over environmental pollution. Nowadays, although most research efforts are focused on ...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s10295-011-0999-3
更新日期：2011-09-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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doi：10.1007/s10295-018-2103-8
更新日期：2019-01-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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doi：10.1007/s10295-008-0472-0
更新日期：2009-01-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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doi：10.1007/s10295-009-0678-9
更新日期：2010-04-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2020-11-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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abstract：:Biological synthesis of plant secondary metabolites has attracted increasing attention due to their proven or assumed beneficial properties and health-promoting effects. Phenylpropanoids are the precursors to a range of important plant metabolites such as the secondary metabolites belonging to the flavonoid/stilbenoid...
journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2011-10-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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