Spatial expression of a mercury-inducible green fluorescent protein within a nanoporous latex-based biosensor coating.


:Optimizing the reactivity of cell coatings developed as biosensors or biocatalysts requires measurements of gene expression in the immobilized cells. To quantify and localize gene expression within a latex-based mercury biosensor, a plasmid, pmerGFP, was constructed, which contains the green fluorescent protein (GFP) gene under transcriptional control of the mercury resistance operon regulatory sequences. When cells containing this plasmid were exposed to mercuric chloride, GFP synthesis was induced and could be quantified by fluorescence. E. coli strain JM109 (pmerGFP) was mixed with SF091 latex (Rohm & Haas), Tween 20, and glycerol, and coated as an approximate 20-microm thick nanoporous adhesive coating on a polyester substrate. The cell coat was overlaid with a nanoporous topcoat of latex, Tween 20, and glycerol. Different fluorescent microspheres were used to mark the topcoat and cell coat layers of the coating. Upon exposure to mercury(II), cells within the coating were induced to synthesize GFP, and laser scanning confocal microscopy was used to quantify expression spatially within the cell coat. GFP expression in the coatings increased with increasing mercury concentration (2-20 microM), temperature (21-37 degrees C), and time of incubation (0-39 h). There was a gradient of GFP expression through the cell coat with expression higher near the topcoat-cell coat interface relative to the bottom of the cell coat. The topcoat thickness did not significantly affect GFP expression indicating that diffusion of mercury(II) and oxygen through the topcoat was not limiting.


Schottel JL,Orwin PM,Anderson CR,Flickinger MC




Has Abstract


2008-04-01 00:00:00












  • Construction of the astaxanthin biosynthetic pathway in a methanotrophic bacterium Methylomonas sp. strain 16a.

    abstract::Methylomonas sp. strain 16a is an obligate methanotrophic bacterium that uses methane or methanol as the sole carbon source. An effort was made to engineer this organism for astaxanthin production. Upon expressing the canthaxanthin gene cluster under the control of the native hps promoter in the chromosome, canthaxant...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Ye RW,Yao H,Stead K,Wang T,Tao L,Cheng Q,Sharpe PL,Suh W,Nagel E,Arcilla D,Dragotta D,Miller ES

    更新日期:2007-04-01 00:00:00

  • A novel high-throughput and quantitative method based on visible color shifts for screening Bacillus subtilis THY-15 for surfactin production.

    abstract::A novel chromatic visible screening method using bromothymol blue (BTB) as a color indicator and cetylpyridinium chloride (CPC) as a mediator was constructed to obtain the high titer surfactin-producing strains. The reliability and quantification accuracy of color shift were also confirmed. Regular chromatic responses...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Yang H,Yu H,Shen Z

    更新日期:2015-08-01 00:00:00

  • Improvement of bioremediation by Pseudomonas and Burkholderia by mutants of the Vitreoscilla hemoglobin gene (vgb) integrated into their chromosomes.

    abstract::Using genetic engineering, the Vitreoscilla (bacterial) hemoglobin gene (vgb) was integrated stably into the chromosomes of Pseudomonas aeruginosa and Burkholderia sp. strain DNT. This was done for both wild type vgb and two site-directed mutants of vgb that produce Vitreoscilla hemoglobin (VHb) with lowered oxygen af...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Kim Y,Webster DA,Stark BC

    更新日期:2005-04-01 00:00:00

  • Enhanced production of amidase from Rhodococcus erythropolis MTCC 1526 by medium optimisation using a statistical experimental design.

    abstract::In the present work, statistical experimental methodology was used to enhance the production of amidase from Rhodococcus erythropolis MTCC 1526. R. erythropolis MTCC 1526 was selected through screening of seven strains of Rhodococcus species. The Placket-Burman screening experiments suggested that sorbitol as carbon s...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Vaidya BK,Mutalik SR,Joshi RM,Nene SN,Kulkarni BD

    更新日期:2009-05-01 00:00:00

  • Colanic acid is an exopolysaccharide common to many enterobacteria isolated from paper-machine slimes.

    abstract::In this study, polysaccharide-producing bacteria were isolated from slimes collected from two Finnish and one Spanish paper mill and the exopolysaccharides (EPSs) produced by 18 isolates were characterised. Most of the isolates, selected on the bases of slimy colony morphology, were members of the family Enterobacteri...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Rättö M,Verhoef R,Suihko ML,Blanco A,Schols HA,Voragen AG,Wilting R,Siika-Aho M,Buchert J

    更新日期:2006-05-01 00:00:00

  • Production and genetic improvement of a novel antimycotic agent, saadamycin, against dermatophytes and other clinical fungi from endophytic Streptomyces sp. Hedaya48.

    abstract::As a part of our ongoing efforts towards finding novel antimycotic agents from marine microflora of the Red Sea, vanillin, 5,7-dimethoxy-4-p-methoxylphenylcoumarin and the new antimycotic compound saadamycin were isolated from endophytic Streptomyces sp. Hedaya48. The producing strain was isolated from the Egyptian sp...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: El-Gendy MM,El-Bondkly AM

    更新日期:2010-08-01 00:00:00

  • Evaluation of the catalytic specificity, biochemical properties, and milk clotting abilities of an aspartic peptidase from Rhizomucor miehei.

    abstract::In this study, we detail the specificity of an aspartic peptidase from Rhizomucor miehei and evaluate the effects of this peptidase on clotting milk using the peptide sequence of k-casein (Abz-LSFMAIQ-EDDnp) and milk powder. Molecular mass of the peptidase was estimated at 37 kDa, and optimum activity was achieved at ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: da Silva RR,Souto TB,de Oliveira TB,de Oliveira LC,Karcher D,Juliano MA,Juliano L,de Oliveira AH,Rodrigues A,Rosa JC,Cabral H

    更新日期:2016-08-01 00:00:00

  • Production and characterisation of a biosurfactant isolated from Pseudomonas aeruginosa UW-1.

    abstract::Pseudomonas aeruginosa UW-1 produced 17-24 g L-1 rhamnolipid in vegetable oil-containing media in shake flask cultures in 13 days. In time course studies of growth and rhamnolipid production in a salts medium containing 6% canola oil, total bacterial count reached 2.6 x 10(10) CFU ml-1 after 48 h and a maximum rhamnol...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Sim L,Ward OP,Li ZY

    更新日期:1997-10-01 00:00:00

  • Corrections to: Enhanced lincomycin production by co‑overexpression of metK1 and metK2 in Streptomyces lincolnensis.

    abstract::In the online published article, row value "pIB139-metK1-metK2" in table 1 has been processed incorrectly. The correct table is given below. ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,已发布勘误


    authors: Xu Y,Tan G,Ke M,Li J,Tang Y,Meng S,Niu J,Wang Y,Liu R,Wu H,Bai L,Zhang L,Zhang B

    更新日期:2018-06-01 00:00:00

  • An economically and environmentally acceptable synthesis of chiral drug intermediate L-pipecolic acid from biomass-derived lysine via artificially engineered microbes.

    abstract::Deficiency in petroleum resources and increasing environmental concerns have pushed a bio-based economy to be built, employing a highly reproducible, metal contaminant free, sustainable and green biomanufacturing method. Here, a chiral drug intermediate L-pipecolic acid has been synthesized from biomass-derived lysine...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Cheng J,Huang Y,Mi L,Chen W,Wang D,Wang Q

    更新日期:2018-06-01 00:00:00

  • Regioselective aromatic hydroxylation of quinaldine by water using quinaldine 4-oxidase in recombinant Pseudomonas putida.

    abstract::Biocatalytic hydrocarbon oxyfunctionalizations are typically accomplished using oxygenases in living bacteria as biocatalysts. These processes are often limited by either oxygen mass transfer, cofactor regeneration, and/or enzyme instabilities due to the formation of reactive oxygen species. Here, we discuss an altern...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Utkür FO,Gaykawad S,Bühler B,Schmid A

    更新日期:2011-08-01 00:00:00

  • Production of beta-glucosidase using immobilised Piromyces sp. KSX1 and Orpinomyces sp. 478P1 in repeat-batch culture.

    abstract::Two anaerobic fungi, one a monocentric strain ( Piromyces sp. KSX1) and the other a polycentric strain ( Orpinomyces sp. 478P1), were immobilised in calcium alginate beads and cultured in sequential batches where spent medium (containing 0.25% cellobiose) was repeatedly drained and replaced. beta-Glucosidase productio...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: McCabe BK,Kuek C,Gordon GL,Phillips MW

    更新日期:2003-04-01 00:00:00

  • Identification of bacteria contaminating pulp and a paper machine in a Canadian paper mill.

    abstract::Over 100 bacteria from pulp and slime samples in a Canadian paper mill were identified by partial sequencing of their 16S rDNAs. Seventy-one percent of the isolates could be assigned to a bacterial genus with a high level of confidence. Another 12% exhibited at least 95% similarity within their 16S rDNA sequence with ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Desjardins E,Beaulieu C

    更新日期:2003-03-01 00:00:00

  • Linking species concepts to natural product discovery in the post-genomic era.

    abstract::A widely accepted species concept for bacteria has yet to be established. As a result, species designations are inconsistently applied and tied to what can be considered arbitrary metrics. Increasing access to DNA sequence data and clear evidence that bacterial genomes are dynamic entities that include large numbers o...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Jensen PR

    更新日期:2010-03-01 00:00:00

  • Production of raw cassava starch-degrading enzyme by Penicillium and its use in conversion of raw cassava flour to ethanol.

    abstract::A newly isolated strain Penicillium sp. GXU20 produced a raw starch-degrading enzyme which showed optimum activity towards raw cassava starch at pH 4.5 and 50 °C. Maximum raw cassava starch-degrading enzyme (RCSDE) activity of 20 U/ml was achieved when GXU20 was cultivated under optimized conditions using wheat bran (...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Lin HJ,Xian L,Zhang QJ,Luo XM,Xu QS,Yang Q,Duan CJ,Liu JL,Tang JL,Feng JX

    更新日期:2011-06-01 00:00:00

  • Microarray analysis of p-anisaldehyde-induced transcriptome of Saccharomyces cerevisiae.

    abstract::p-Anisaldehyde (4-methoxybenzaldehyde), an extract from Pimpinella anisum L. seeds, is a potential novel preservative. To reveal the possible action mechanism of p-anisaldehyde against microorganisms, yeast-based commercial oligonucleotide microarrays were used to analyze the genome-wide transcriptional changes in res...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Yu L,Guo N,Yang Y,Wu X,Meng R,Fan J,Ge F,Wang X,Liu J,Deng X

    更新日期:2010-03-01 00:00:00

  • A novel regeneration of iron citrate solution by biooxidation of iron-oxidizing bacteria.

    abstract::Liquid phase oxidation process using chelated iron solution is among the most promising techniques for the hydrogen sulfide removal due to its double advantage of waste minimization and resource recovery. Regeneration of chelated iron is a core reaction in this process. Regeneration of chelated iron in acidic solution...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Wang YJ,Li DP,Liu C,Zhan GQ,He XH

    更新日期:2014-11-01 00:00:00

  • Engineering Yarrowia lipolytica for the production of cyclopropanated fatty acids.

    abstract::Traditional synthesis of biodiesel competes with food sources and has limitations with storage, particularly due to limited oxidative stability. Microbial synthesis of lipids provides a platform to produce renewable fuel with improved properties from various renewable carbon sources. Specifically, biodiesel properties...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Markham KA,Alper HS

    更新日期:2018-10-01 00:00:00

  • Distribution and diversity of Dipteran-specific cry and cyt genes in native Bacillus thuringiensis strains obtained from different ecosystems of Iran.

    abstract::One hundred and twenty-eight Bacillus thuringiensis isolates from fields of different ecological regions of Iran were collected to study the distribution and diversity of Dipteran-specific cry and cyt genes. The percentage of samples with Bt showed significant differences between different regions and also between dif...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Jouzani GS,Abad AP,Seifinejad A,Marzban R,Kariman K,Maleki B

    更新日期:2008-02-01 00:00:00

  • A very efficient bioconversion of soybean phytosterols mixtures to androstanes by mycobacteria.

    abstract::The production of several high value steroid drugs, used as progestational, adrenocortical, estrogenic and contraceptive agents, is mostly derived from 4-androstene-dione (AD) and 1,4 androsta-diene-3,17-dione (ADD). Three Vietnamese phytosterols mixtures named VN-1, VN-2 and VN-3, isolated from soybean oil may be eff...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Pérez C,Falero A,Duc HL,Balcinde Y,Hung BR

    更新日期:2006-08-01 00:00:00

  • A recombinant vaccine against hydatidosis: production of the antigen in Escherichia coli.

    abstract::A commercial process was developed for producing a recombinant vaccine against hydatidosis in farm animals. The vaccine antigen consisting of a surface protein of the oncospheres of the hydatid worm (Echinococcus granulosus), was produced as inclusion bodies in Escherichia coli. Fed-batch cultures of E. coli using Ter...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Manderson D,Dempster R,Chisti Y

    更新日期:2006-03-01 00:00:00

  • Effect of dissolved oxygen on L-methionine production from glycerol by Escherichia coli W3110BL using metabolic flux analysis method.

    abstract::L-Methionine is an essential amino acid in humans, which plays an important role in the synthesis of some important amino acids and proteins. In this work, metabolic flux of batch fermentation of L-methionine with recombinant Escherichia coli W3110BL was analyzed using the flux balance analysis method, which estimated...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Niu K,Xu YY,Wu WJ,Zhou HY,Liu ZQ,Zheng YG

    更新日期:2020-03-01 00:00:00

  • Solar Pond devices: free energy or bioreactors for Artemia biomass production?

    abstract::The recent exponential growth in industrial aquaculture has led to a huge increase in Artemia biomass production in order to meet increased fish production needs. The present study explores the potential use of salt gradient solar ponds (SGSPs) for production of Artemia nauplii. An SGSP is a basin of water where solar...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Gouveia L,Sousa J,Marques A,Tavares C,Giestas M

    更新日期:2009-08-01 00:00:00

  • Antifungal activity of Bacillus subtilis 355 against wood-surface contaminant fungi.

    abstract::A strain of Bacillus subtilis was examined for antifungal activity against phytopathogenic and wood-surface contaminant fungi. The bacterium was grown in five culture media with different incubation times in order to study cell development, sporulation, and the production of metabolites with antifungal activity. The a...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Feio SS,Barbosa A,Cabrita M,Nunes L,Esteves A,Roseiro JC,Curto MJ

    更新日期:2004-06-01 00:00:00

  • Electrochemical behavior of the 316L steel type in a marine culture of microalgae (Porphyridium purpureum) under the 12/12 h photoperiod and effect of different working electrode exposure conditions on the biofilm-metal interface.

    abstract::The industrial crops of microalgae use processes calling upon the presence of parts of metal nature such as steel 316L type. The goal of this study is to test the electrochemical behavior of this material in a marine culture of microalgae. Porphyridium purpureum was used under a photoperiod of alternation darkness/lig...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Djemai-Zoghlache Y,Isambert A,Belhaneche-Bensemra N

    更新日期:2011-12-01 00:00:00

  • Xylanase II from Trichoderma reesei QM 9414: conformational and catalytic stability to Chaotropes, Trifluoroethanol, and pH changes.

    abstract::Xylanase II, a key enzyme in the hydrolysis of xylan, was purified from cultures of Trichoderma reesei QM 9414 (anamorph of Hypocrea jecorina) grown on wheat straw as a carbon source. Xylanase treated with increasing guanidinium hydrochloride concentrations was denatured in a cooperative way regarding secondary and te...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: López G,Bañares-Hidalgo A,Estrada P

    更新日期:2011-01-01 00:00:00

  • Molecular and phenotypic comparison of phaeochromycin-producing strains of Streptomyces phaeochromogenes and Streptomyces ederensis.

    abstract::Streptomyces strain LL-P018 produces the phaeochromycins, novel anti-inflammatory polyketides. This organism was identified as a strain of Streptomyces phaeochromogenes by physiological and genetic taxonomic analysis. In order to gain greater taxonomic perspective, LL-P018 was compared to related strains from major cu...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Ritacco FV,Eveleigh DE

    更新日期:2008-09-01 00:00:00

  • Colonization of contaminated soil by an introduced bacterium: effects of initial pentachlorophenol levels on the survival of Sphingomonas chlorophenolica strain RA2.

    abstract::The survival of a Sphingomonas species that was introduced into pentachlorophenol (PCP)-contaminated soil was monitored with two complementary methods, a respiration-based assay and a most probable number (MPN) technique. Sphingomonas chlorophenolicastrain RA2 is a PCP-mineralizing bacterium that was introduced into s...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Colores GM,Schmidt SK

    更新日期:1999-10-01 00:00:00

  • The mycinose-biosynthetic genes of Streptomyces fradiae, producer of tylosin.

    abstract::The tylE-J region of the tylosin-biosynthetic gene cluster of Streptomyces fradiae contains six open reading frames. The products of tylJ and tylD are nucleoside diphospho (NDP)-deoxyhexose 3-epimerase and NDP-deoxyhexose 4-ketoreductase, respectively, involved in the synthesis of NDP-6-deoxyallose from NDP-4-keto, 6-...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Bate N,Cundliffe E

    更新日期:1999-08-01 00:00:00

  • Synthesis of 4-nitrophenyl acetate using molecular sieve-immobilized lipase from Bacillus coagulans.

    abstract::Extracellular lipase from Bacillus coagulans BTS-3 was immobilized on (3 A x 1.5 mm) molecular sieve. The molecular sieve showed approximately 68.48% binding efficiency for lipase (specific activity 55 IU mg(-1)). The immobilized enzyme achieved approx 90% conversion of acetic acid and 4-nitrophenol (100 mM each) into...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Raghuvanshi S,Gupta R

    更新日期:2009-03-01 00:00:00