Abstract:
:Clostridium saccharoperbutylacetonicum N1-4 (Csa) is a historically significant anaerobic bacterium which can perform saccharolytic fermentations to produce acetone, butanol, and ethanol (ABE). Recent genomic analyses have highlighted this organism's potential to produce polyketide and nonribosomal peptide secondary metabolites, but little is known regarding the identity and function of these metabolites. This study provides a detailed bioinformatic analysis of seven biosynthetic gene clusters (BGCs) present in the Csa genome that are predicted to produce polyketides/nonribosomal peptides. An RNA-seq-based untargeted transcriptomic approach revealed that five of seven BGCs were expressed during ABE fermentation. Additional characterization of a highly expressed nonribosomal peptide synthetase gene led to the discovery of its associated metabolite and its biosynthetic pathway. Transcriptomic analysis suggested an association of this nonribosomal peptide synthetase gene with butanol tolerance, which was supported by butanol challenge assays.
journal_name
J Ind Microbiol Biotechnoljournal_title
Journal of industrial microbiology & biotechnologyauthors
Li JS,Barber CC,Herman NA,Cai W,Zafrir E,Du Y,Zhu X,Skyrud W,Zhang Wdoi
10.1007/s10295-020-02266-8subject
Has Abstractpub_date
2020-03-01 00:00:00pages
319-328issue
3eissn
1367-5435issn
1476-5535pii
10.1007/s10295-020-02266-8journal_volume
47pub_type
杂志文章abstract::A polyene compound NPP identified in Pseudonocardia autotrophica was shown to contain an aglycone identical to nystatin, but to harbor a unique disaccharide moiety that led to higher solubility and reduced hemolytic activity. Recently, it was revealed that the final step of NPP (nystatin-like polyene) biosynthesis is ...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-016-1813-z
更新日期:2016-11-01 00:00:00
abstract::The Red recombinase system, the most convenient genetic tool applied in Escherichia coli and other bacteria, was introduced for gene replacement in Klebsiella pneumoniae. The novel K. pneumoniae gene replacement system comprised the Red and FLP recombinases expression vector pDK6-red and pDK6-flp, and linear DNA fragm...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-012-1117-x
更新日期:2012-08-01 00:00:00
abstract::Component C (Acarviosy-1,4-Glc-1,1-Glc) was a highly structural acarbose analog, which could be largely formed during acarbose fermentation process, resulting in acarbose purification being highly difficult. By choosing osmolality level as the key fermentation parameter of acarbose-producing Actinoplanes sp. A56, this...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-014-1520-6
更新日期:2014-12-01 00:00:00
abstract::The present study deals with the production of citric acid from a ram horn peptone (RHP) by Aspergillus niger NRRL 330. A medium from RHP and a control medium (CM) were compared for citric acid production using A. niger in a batch culture. For this purpose, first, RHP was produced. Ram horns were hydrolyzed by treatme...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-004-0147-4
更新日期:2004-07-01 00:00:00
abstract::Given the recent increases in natural gas reserves and associated drawbacks of current gas-to-liquids technologies, the development of a bioconversion process to directly convert methane to liquid fuels would generate considerable industrial interest. Several clades of anaerobic methanotrophic archaea (ANME) are capab...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s10295-014-1548-7
更新日期:2015-03-01 00:00:00
abstract::Study of the distribution of the oxygen mass transfer coefficient, k (l) a, for a stirred bioreactor and simulated (pseudoplastic solutions of carboxymethylcellulose sodium salt) bacterial (P. shermanii), yeast (S. cerevisiae), and fungal (P. chrysogenum free mycelia) broths indicated significant variation of transfer...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-010-0930-3
更新日期:2011-09-01 00:00:00
abstract::Providencia rettgeri strain YL was found to be efficient in heterotrophic nitrogen removal under aerobic conditions. Maximum removal of NH(4) (+)-N occurred under the conditions of pH 7 and supplemented with glucose as the carbon source. Inorganic ions such as Mg(2+), Mn(2+), and Zn(2+) largely influenced the growth a...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-010-0708-7
更新日期:2010-06-01 00:00:00
abstract::Traditional synthesis of biodiesel competes with food sources and has limitations with storage, particularly due to limited oxidative stability. Microbial synthesis of lipids provides a platform to produce renewable fuel with improved properties from various renewable carbon sources. Specifically, biodiesel properties...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-018-2067-8
更新日期:2018-10-01 00:00:00
abstract::The ability of dromedary skim milk to form an acid curd during a lactic acid starter fermentation was investigated. The activity of the starter in dromedary milk was characterized by a longer lag phase ( approximately 5 vs. approximately 1 h) and by an earlier decline phase. This suggests the presence of inhibiting fa...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1038/sj.jim.7000111
更新日期:2001-05-01 00:00:00
abstract::A strain of Bacillus subtilis was examined for antifungal activity against phytopathogenic and wood-surface contaminant fungi. The bacterium was grown in five culture media with different incubation times in order to study cell development, sporulation, and the production of metabolites with antifungal activity. The a...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-004-0133-x
更新日期:2004-06-01 00:00:00
abstract::For decades, microbial natural products have been one of the major sources of novel drugs for pharmaceutical companies, and today all evidence suggests that novel molecules with potential therapeutic applications are still waiting to be discovered from these natural sources, especially from actinomycetes. Any appropri...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s10295-010-0882-7
更新日期:2011-03-01 00:00:00
abstract::Biosurfactant-producing bacteria were isolated from the production water of an oil field. Isolates were screened for biosurfactant production using surface tension test. The highest reduction of surface tension was achieved with a bacterial strain which was identified by 16S rRNA gene sequencing as Brevibacilis brevis...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-008-0403-0
更新日期:2008-12-01 00:00:00
abstract::Natural product discovery in the microbial world has historically been biased toward aerobes. Recent in silico analysis demonstrates that genomes of anaerobes encode unexpected biosynthetic potential for natural products, however, chemical data on natural products from the anaerobic world are extremely limited. Here, ...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s10295-018-2086-5
更新日期:2019-03-01 00:00:00
abstract::A collection of Pseudomonas corrugata and P. mediterranea strains, two closely related species, was evaluated for the presence and variability of pha loci. Using PCR methods that specifically amplify segments of medium-chain-length poly(hydroxyalkanoate) (mcl-PHA) synthase genes, we demonstrated the presence of phaC1 ...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-005-0205-6
更新日期:2005-02-01 00:00:00
abstract::The aspartate pathway of Streptomyces clavuligerus is an important primary metabolic pathway which provides substrates for beta-lactam synthesis. In this study, the hom gene which encodes homoserine dehydrogenase was cloned from the cephamycin C producer S. clavuligerus NRRL 3585 and characterized. The fully sequenced...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-007-0259-8
更新日期:2008-01-01 00:00:00
abstract::Several indigenous Saccharomyces strains from musts were isolated in the Jerez de la Frontera region, at the end of spontaneous fermentation, in order to select the most suitable autochthonous yeast starter, during the 2007 vintage. Five strains were chosen for their oenological abilities and fermentative kinetics to ...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-013-1251-0
更新日期:2013-06-01 00:00:00
abstract::Pentose sugars are increasingly being used in industrial applications of Saccharomyces cerevisiae. Although L-arabinose is a highlighted pentose that has been identified as next-generation biomass, arabinose fermentation has not yet undergone extensive development for industrial utilization. In this study, we integrat...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-019-02233-y
更新日期:2019-12-01 00:00:00
abstract::In this study, the M13 primer was used to distinguish Geotrichum candidum from the anamorphic and teleomorphic forms of other arthrospore-forming species (discriminatory power = 0.99). For intraspecific characterization, the GATA4 primer showed the highest level of discrimination for G. candidum among the 20 microsate...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-006-0130-3
更新日期:2006-12-01 00:00:00
abstract::Genomic analysis of the clavulanic acid (CA)-high-producing Streptomyces clavuligerus strains, OL13 and OR, developed through random mutagenesis revealed a frameshift mutation in the cas1 gene-encoding clavaminate synthase 1. Overexpression of the intact cas1 in S. clavuligerus OR enhanced the CA titer by approximatel...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-019-02196-0
更新日期:2019-08-01 00:00:00
abstract::The effects of different straw types and organic and inorganic nitrogen (N) sources on the chemical composition and odor concentration (OC) of mushroom composting emissions, compost parameters, and mushroom yield were examined using bench-scale and large-scale (windrows and aerated tunnels) composting systems. There w...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1038/sj.jim.7000292
更新日期:2002-09-01 00:00:00
abstract::The genome of streptomycetes has the ability to produce many novel and potentially useful bioactive compounds, but most of which are not produced under standard laboratory cultivation conditions and are referred to as silent/cryptic secondary metabolites. Streptomyces lavendulae FRI-5 produces several types of bioacti...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-017-1997-x
更新日期:2018-02-01 00:00:00
abstract::Development of natural products for therapeutic use is often hindered by limited availability of material from producing organisms. The speed at which current technologies enable the cloning, sequencing, and manipulation of secondary metabolite genes for production of novel compounds has made it impractical to optimiz...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-003-0045-1
更新日期:2003-08-01 00:00:00
abstract::Acid blue-15, a complex and resonance-stabilized triphenylmethane (TPM) textile dye, resistant to transformation, was decolorized/degraded in an up-flow immobilized cell bioreactor. A consortium comprised of isolates belonging to Bacillus sp., Alcaligenes sp. and Aeromonas sp. formed a multispecies biofilm on refracto...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-004-0121-1
更新日期:2004-03-01 00:00:00
abstract::Cyanobacteria are commonly used for the phytostimulation and biofertilization of agriculture crops due to their nitrogen-fixing ability. However, the contribution by their phytohormones has been neglected. This study focuses on the screening of rhizospheric and free-living cyanobacteria for in vitro phytohormones prod...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-010-0833-3
更新日期:2011-01-01 00:00:00
abstract::Since the discovery of the first antibiotic, natural products have played an important role in chemistry, biology and medicine. To explore the potential of bioactive compounds from microbes isolated from the southeast of Tibet, China, a crude extract library was constructed and screened against Staphylococcus aureus. ...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-017-1908-1
更新日期:2017-05-01 00:00:00
abstract::Plant cell walls are comprised of cellulose, hemicellulose and other polymers that are intertwined. This complex structure acts as a barrier to degradation by single enzyme. Thus, a cocktail consisting of bi and multifunctional xylanases and xylan debranching enzymes is most desired combination for the efficient utili...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s10295-008-0342-9
更新日期:2008-07-01 00:00:00
abstract::Rapamycin is an immunosuppressive metabolite produced from several actinomycete species. Besides its immunosuppressive activity, rapamycin and its analogs have additional therapeutic potentials, including antifungal, antitumor, neuroprotective/neuroregenerative, and lifespan extension activities. The core structure of...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s10295-016-1834-7
更新日期:2017-05-01 00:00:00
abstract::Natural products are a crucial source of antimicrobial agents, but reliance on low-resolution bioactivity-guided approaches has led to diminishing interest in discovery programmes. Here, we demonstrate that two in-house automated informatic platforms can be used to target classes of biologically active natural product...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-015-1675-9
更新日期:2016-03-01 00:00:00
abstract::A widely accepted species concept for bacteria has yet to be established. As a result, species designations are inconsistently applied and tied to what can be considered arbitrary metrics. Increasing access to DNA sequence data and clear evidence that bacterial genomes are dynamic entities that include large numbers o...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s10295-009-0683-z
更新日期:2010-03-01 00:00:00
abstract::In the online published article, row value "pIB139-metK1-metK2" in table 1 has been processed incorrectly. The correct table is given below. ...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章,已发布勘误
doi:10.1007/s10295-018-2036-2
更新日期:2018-06-01 00:00:00