Abstract:
:Natural products are a crucial source of antimicrobial agents, but reliance on low-resolution bioactivity-guided approaches has led to diminishing interest in discovery programmes. Here, we demonstrate that two in-house automated informatic platforms can be used to target classes of biologically active natural products, specifically, peptaibols. We demonstrate that mass spectrometry-based informatic approaches can be used to detect natural products with high sensitivity, identifying desired agents present in complex microbial extracts. Using our specialised software packages, we could elaborate specific branches of chemical space, uncovering new variants of trichopolyn and demonstrating a way forward in mining natural products as a valuable source of potential pharmaceutical agents.
journal_name
J Ind Microbiol Biotechnoljournal_title
Journal of industrial microbiology & biotechnologyauthors
Johnston CW,Connaty AD,Skinnider MA,Li Y,Grunwald A,Wyatt MA,Kerr RG,Magarvey NAdoi
10.1007/s10295-015-1675-9subject
Has Abstractpub_date
2016-03-01 00:00:00pages
293-8issue
2-3eissn
1367-5435issn
1476-5535pii
10.1007/s10295-015-1675-9journal_volume
43pub_type
杂志文章abstract::A 2 M sodium acetate buffer at pH 4.2 was tried to simplify the step of pH adjustment in a laboratory dry-grind procedure. Ethanol yields or conversion efficiencies of 18 sorghum hybrids improved significantly with 2.0-5.9% (3.9% on average) of relative increases when the method of pH adjustment changed from tradition...
journal_title:Journal of industrial microbiology & biotechnology
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abstract::Electropermeabilization of Clostridium cellulolyticum was optimized using ATP leakage assays. Electrotransformation was then performed under optimized conditions (6 to 7.5 kV x cm(-1) field strength applied during 5 ms to a mixture containing methylated plasmids and late exponential phase cell suspensions (10 molecule...
journal_title:Journal of industrial microbiology & biotechnology
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doi:10.1038/sj.jim.7000081
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journal_title:Journal of industrial microbiology & biotechnology
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journal_title:Journal of industrial microbiology & biotechnology
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abstract::A formate dehydrogenase gene (fdh) originated from Candida boidinii was co-expressed in E. coli BL21 (DE3) with the cyclohexanone monooxygenase gene (chmo) cloned from Acinetobacter calcoaceticus NCIMB 9871. The co-expression system was then used as a whole-cell biocatalyst to synthesize chiral phenyl methyl sulfoxide...
journal_title:Journal of industrial microbiology & biotechnology
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更新日期:2015-08-01 00:00:00
abstract::p-Anisaldehyde (4-methoxybenzaldehyde), an extract from Pimpinella anisum L. seeds, is a potential novel preservative. To reveal the possible action mechanism of p-anisaldehyde against microorganisms, yeast-based commercial oligonucleotide microarrays were used to analyze the genome-wide transcriptional changes in res...
journal_title:Journal of industrial microbiology & biotechnology
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abstract::The operation of bioreactors and the metabolism of microorganisms in biofilms or soil/sediment systems are strongly dictated by the transport processes therein. Nuclear magnetic resonance (NMR) spectroscopy or magnetic resonance imaging (MRI) allow nondestructive and noninvasive quantification and visualisation (in ca...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1038/sj/jim/7000087
更新日期:2001-01-01 00:00:00
abstract::Low-energy ion beam irradiation (10-200 keV) has been proved to have a wide range of biological effects in recent years. When Rhizopus oryzae PW352 was irradiated with a 15-keV low-energy ion beam an L(+)-lactic acid high-yield mutant, RQ4015, was obtained. When 150 g/l glucose was used as the sole carbon source, L(+)...
journal_title:Journal of industrial microbiology & biotechnology
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doi:10.1007/s10295-009-0621-0
更新日期:2009-11-01 00:00:00
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journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-009-0678-9
更新日期:2010-04-01 00:00:00
abstract::Carotenoids represent a group of valuable molecules for the pharmaceutical, chemical, food and feed industries, not only because they can act as vitamin A precursors, but also for their coloring, antioxidant and possible tumor-inhibiting activity. Animals cannot synthesize carotenoids, and these pigments must therefor...
journal_title:Journal of industrial microbiology & biotechnology
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doi:10.1007/s10295-008-0492-9
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abstract::A closed-circuit microbial fuel cell (C-MFC) was operated to investigate the electron flux under fed-batch mode, and the results were compared to those of open-circuit MFC (O-MFC) and a fermentation reactor (F-reactor). The current was the largest electron sink (52.7% of influent SCOD) in C-MFC, whereas biomass and me...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
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更新日期:2015-07-01 00:00:00
abstract::Controlling water activity (a(w)) can significantly impact the growth of Salmonella in poultry litter and manure - a phenomenon that was studied quantitatively using two common serotypes of Salmonella. The quantitative effect of changes in levels of a(w) on Salmonella populations was determined using inoculated, frost...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1038/sj.jim.7000116
更新日期:2001-04-01 00:00:00
abstract::In this study, the M13 primer was used to distinguish Geotrichum candidum from the anamorphic and teleomorphic forms of other arthrospore-forming species (discriminatory power = 0.99). For intraspecific characterization, the GATA4 primer showed the highest level of discrimination for G. candidum among the 20 microsate...
journal_title:Journal of industrial microbiology & biotechnology
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abstract::The use of proteomics for direct detection of expressed pathways producing natural products has yielded many new compounds, even when used in a screening mode without a bacterial genome sequence available. Here we quantify the advantages of having draft DNA-sequence available for strain-specific proteomics using the l...
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abstract::A metabolic network consisting of 48 reactions was established to describe intracellular processes during growth and poly-3-hydroxybutyrate (PHB) production for Cupriavidus necator DSM 545. Glycerol acted as the sole carbon source during exponential, steady-state cultivation conditions. Elementary flux modes were obta...
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更新日期:1997-02-01 00:00:00
abstract::In the online published article, row value "pIB139-metK1-metK2" in table 1 has been processed incorrectly. The correct table is given below. ...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章,已发布勘误
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更新日期:2018-06-01 00:00:00
abstract::Robust microorganisms are necessary for economical bioethanol production. However, such organisms must be able to effectively ferment both hexose and pentose sugars present in lignocellulosic hydrolysate to ethanol. Wild type Saccharomyces cerevisiae can rapidly ferment hexose, but cannot ferment pentose sugars. Consi...
journal_title:Journal of industrial microbiology & biotechnology
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更新日期:2011-05-01 00:00:00
abstract::Providencia rettgeri strain YL was found to be efficient in heterotrophic nitrogen removal under aerobic conditions. Maximum removal of NH(4) (+)-N occurred under the conditions of pH 7 and supplemented with glucose as the carbon source. Inorganic ions such as Mg(2+), Mn(2+), and Zn(2+) largely influenced the growth a...
journal_title:Journal of industrial microbiology & biotechnology
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journal_title:Journal of industrial microbiology & biotechnology
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更新日期:2008-11-01 00:00:00
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journal_title:Journal of industrial microbiology & biotechnology
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abstract::A membrane bioreactor for production of nisin Z was constructed using Lactococcus lactis IO-1 in continuous culture using hydrolyzed sago starch as carbon source. A strategy used to enhance the productivity of nisin Z was to maintain the cells in a continuous growth at high cell concentration. This resulted in a volum...
journal_title:Journal of industrial microbiology & biotechnology
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更新日期:2009-03-01 00:00:00
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journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-014-1437-0
更新日期:2014-06-01 00:00:00
abstract::Klebsiella oxytoca naturally produces a large amount of 2,3-butanediol (2,3-BD), a promising bulk chemical with wide industrial applications, along with various byproducts. In this study, the in silico gene knockout simulation of K. oxytoca was carried out for 2,3-BD overproduction by inhibiting the formation of bypro...
journal_title:Journal of industrial microbiology & biotechnology
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更新日期:2013-09-01 00:00:00
abstract::The ability of dromedary skim milk to form an acid curd during a lactic acid starter fermentation was investigated. The activity of the starter in dromedary milk was characterized by a longer lag phase ( approximately 5 vs. approximately 1 h) and by an earlier decline phase. This suggests the presence of inhibiting fa...
journal_title:Journal of industrial microbiology & biotechnology
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