Abstract：
:Human-like collagen (HLC) is a novel biomedical material with promising applications. Usually, insoluble HLC was formed due to over-expression. In order to improve the production of soluble HLC, the effective chaperone proteins and their mediation roles on HLC were clarified. Trigger factor (TF) pathway with low specificity and high binding affinity to nascent chains could increase soluble HLC expression; GroEL-GroES could increase the expression level of HLC by assisting the correct folding of HLC and increase mRNA level of the gene coding for HLC by enhancing mRNA stability. DnaK chaperone system did not work positively on soluble HLC due to the unbalanced ratio of DnaK:DnaJ:GrpE, especially too high GrpE significantly inhibited DnaK-mediated refolding. The production of soluble HLC with co-expression of exogenous TF and GroEL-GroES was increased by 35.3 % in comparison with the highest value 0.26 g/L reported previously.
journal_name
J Ind Microbiol Biotechnoljournal_title
Journal of industrial microbiology & biotechnologyauthors
Jia Q,Luo Ydoi
10.1007/s10295-014-1500-xsubject
Has Abstractpub_date
2014-11-01 00:00:00pages
1667-75issue
11eissn
1367-5435issn
1476-5535journal_volume
41pub_type
杂志文章abstract：:Nine thraustochytrid strains isolated from subtropical mangroves were screened for their eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) production potential in a glucose yeast extract medium. Their ability to utilize okara (soymilk residue) for growth and EPA and DHA production was also evaluated. EPA yiel...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1038/sj.jim.7000169
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abstract：:Amyris is a fermentation product company that leverages synthetic biology and has been bringing novel fermentation products to the market since 2009. Driven by breakthroughs in genome editing, strain construction and testing, analytics, automation, data science, and process development, Amyris has commercialized nine ...
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abstract：:L-Methionine is an essential amino acid in humans, which plays an important role in the synthesis of some important amino acids and proteins. In this work, metabolic flux of batch fermentation of L-methionine with recombinant Escherichia coli W3110BL was analyzed using the flux balance analysis method, which estimated...
journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2020-03-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-009-0592-1
更新日期：2009-08-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s10295-015-1636-3
更新日期：2016-03-01 00:00:00
abstract：:This paper summarizes research results and their industrial applications obtained by continuous culture in the former Czechoslovakia. Past achievements as well as recent trends and developments worldwide are presented. The term "Prague School of continuous culture" is put forward and its international activity is outl...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 历史文章,杂志文章
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更新日期：2010-12-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2015-01-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-016-1737-7
更新日期：2016-05-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1038/sj.jim.2900460
更新日期：1997-09-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-005-0064-1
更新日期：2006-05-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-005-0046-3
更新日期：2006-03-01 00:00:00
abstract：:The Food and Drug Administration has become aware of several instances where supposedly sterile medical surgical products made of Chinese cotton have been found to contain viable Pyronema domesticum. The aim of this research was to determine the gamma and electron beam radiation resistance values for the two dormant p...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1038/sj.jim.7000267
更新日期：2002-08-01 00:00:00
abstract：:In the genome of Streptomyces fradiae, the three tylM genes are codirectional with the upstream gene, tylGV. Although the introduction of transcriptional blocks into the tylM genes revealed that they are normally cotranscribed, expression of tylMI still persisted (albeit at a very low level) when either of the upstrea...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1038/sj/jim/7000223
更新日期：2002-03-01 00:00:00
abstract：:The longer start-up period of the Anammox process is due to the very low cellular yield and growth rates of Anammox bacteria. Nitrite inhibition is considered to be the key factor in the instability of the Anammox process during the operation. However, little attention was paid to the inhibitory effect of pH and free ...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-009-0593-0
更新日期：2009-08-01 00:00:00
abstract：:Nitrate amendment is normally an effective method for sulfide control in oil field-produced waters. However, this approach has occasionally failed to prevent sulfide accumulation, despite the presence of active nitrate-reducing bacterial populations. Here, we report our study of bulk chemical transformations in microc...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-009-0639-3
更新日期：2009-12-01 00:00:00
abstract：:The discharge of bromate, a suspected carcinogen, will be restricted in the near future. To assess the possibility of biotechnological treatment of bromate-containing wastewaters, the removal of bromate by chlorate-reducing microorganisms was studied. The removal of bromate and chlorate was studied in laboratory gas-l...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-004-0188-8
更新日期：2005-01-01 00:00:00
abstract：:Production of recombinant proteins is an industrially important technique in the biopharmaceutical sector. Many recombinant proteins are problematic to generate in a soluble form in bacteria as they readily form insoluble inclusion bodies. Recombinant protein solubility can be enhanced by minimising stress imposed on ...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-014-1489-1
更新日期：2014-09-01 00:00:00
abstract：:In this study, the respiratory activity and carbon usage of the mutant strain of A. vinelandii AT6, impaired in poly-β-hydroxybutyrate (PHB) production, and their relationship with the synthesis of alginate were evaluated. The alginate yield and the specific oxygen uptake rate were higher (2.5-fold and 62 %, respectiv...
journal_title：Journal of industrial microbiology & biotechnology
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doi：10.1007/s10295-016-1774-2
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
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journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2004-06-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2015-10-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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doi：10.1007/s10295-010-0717-6
更新日期：2010-06-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2011-01-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-011-1015-7
更新日期：2012-01-01 00:00:00
abstract：:Deficiency in petroleum resources and increasing environmental concerns have pushed a bio-based economy to be built, employing a highly reproducible, metal contaminant free, sustainable and green biomanufacturing method. Here, a chiral drug intermediate L-pipecolic acid has been synthesized from biomass-derived lysine...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-018-2044-2
更新日期：2018-06-01 00:00:00
abstract：:To improve the thermostability of a mesophilic GH family 10 xylanase, AuXyn10A, from Aspergillus usamii E001, its modification was performed by in silico design. Based on the comparison of B-factor values, a mutant xylanase ATXyn10 was predicted by substituting a segment YP from Tyr(25) to Pro(34) of AuXyn10A with the...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-014-1463-y
更新日期：2014-08-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-006-0130-3
更新日期：2006-12-01 00:00:00
abstract：:The exploration of seven physiologically different white rot fungi potential to produce cellulase, xylanase, laccase, and manganese peroxidase (MnP) showed that the enzyme yield and their ratio in enzyme preparations significantly depends on the fungus species, lignocellulosic growth substrate, and cultivation method....
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-008-0454-2
更新日期：2008-11-01 00:00:00
abstract：:Microbial fermentation of sugars from plant biomass to alcohols represents an alternative to petroleum-based fuels. The optimal biocatalyst for such fermentations needs to overcome hurdles such as high concentrations of alcohols and toxic compounds. Lactic acid bacteria, especially lactobacilli, have high innate alcoh...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-016-1797-8
更新日期：2016-09-01 00:00:00
