13C nuclear magnetic resonance studies on selectively labeled bacterial biofilms.

Abstract:

:Bacterial biofilms of Pseudomonas aeruginosa selectively labeled by introduction of 2-13C-glycerol was studied by solid-state and high-resolution nuclear magnetic resonance. The 13C nuclei were mainly integrated into mannuronate and guluronate, the two monomer units forming the bacterial alginate. The signal for the C5 position of the mannuronate, which was easily identified and well separated from other peaks, was analyzed for molecular mobility. The result indicated a high degree of motional freedom within the molecular network of the alginate. Despite the fact that the alginate was part of a solid aqueous gel phase, the reorientation mechanism of the monomer units came close to isotropic tumbling. Solid-state spectra of biofilms labeled in the described manner may serve as a valuable tool for noninvasive analyses of molecular mobility of the alginate component under various influences, thereby revealing important structural information. In addition, the effect of a monovalent electrolyte (LiCl) on the molecular mobility of alginate fragments in an aqueous solution was studied by determining the spin-lattice relaxation times, line widths and line shapes under variations of the ion concentration. The presence of ions accelerated overall motions but left rapid local motions virtually unaffected.

authors

Mayer C,Lattner D,Schürks N

subject

Has Abstract

pub_date

2001-01-01 00:00:00

pages

62-9

issue

1-2

eissn

1367-5435

issn

1476-5535

journal_volume

26

pub_type

杂志文章
  • Influence of straw types and nitrogen sources on mushroom composting emissions and compost productivity.

    abstract::The effects of different straw types and organic and inorganic nitrogen (N) sources on the chemical composition and odor concentration (OC) of mushroom composting emissions, compost parameters, and mushroom yield were examined using bench-scale and large-scale (windrows and aerated tunnels) composting systems. There w...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1038/sj.jim.7000292

    authors: Noble R,Hobbs PJ,Mead A,Dobrovin-Pennington A

    更新日期:2002-09-01 00:00:00

  • The performance of fungal xylan-degrading enzyme preparations in elemental chlorine-free bleaching for Eucalyptus pulp.

    abstract::Cellulase-free xylan-degrading enzyme preparations from Acrophialophora nainiana, Humicola grisea var. thermoidea and two Trichoderma harzianum strains were used as bleaching agents for Eucalyptus kraft pulp, prior to a chlorine dioxide and alkaline bleaching sequence. In comparison to the control sequence (performed ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1038/sj/jim/7000227

    authors: Medeiros RG,Silva FG Jr,Salles BC,Estelles RS,Filho EX

    更新日期:2002-04-01 00:00:00

  • L(+)-lactic acid production by co-fermentation of glucose and xylose with Rhizopus oryzae obtained by low-energy ion beam irradiation.

    abstract::Low-energy ion beam irradiation (10-200 keV) has been proved to have a wide range of biological effects in recent years. When Rhizopus oryzae PW352 was irradiated with a 15-keV low-energy ion beam an L(+)-lactic acid high-yield mutant, RQ4015, was obtained. When 150 g/l glucose was used as the sole carbon source, L(+)...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-009-0621-0

    authors: Wang P,Li J,Wang L,Tang ML,Yu ZL,Zheng ZM

    更新日期:2009-11-01 00:00:00

  • Discovery of a new diol-containing polyketide by heterologous expression of a silent biosynthetic gene cluster from Streptomyces lavendulae FRI-5.

    abstract::The genome of streptomycetes has the ability to produce many novel and potentially useful bioactive compounds, but most of which are not produced under standard laboratory cultivation conditions and are referred to as silent/cryptic secondary metabolites. Streptomyces lavendulae FRI-5 produces several types of bioacti...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-017-1997-x

    authors: Pait IGU,Kitani S,Roslan FW,Ulanova D,Arai M,Ikeda H,Nihira T

    更新日期:2018-02-01 00:00:00

  • Natural and engineered biosynthesis of nucleoside antibiotics in Actinomycetes.

    abstract::Nucleoside antibiotics constitute an important family of microbial natural products bearing diverse bioactivities and unusual structural features. Their biosynthetic logics are unique with involvement of complex multi-enzymatic reactions leading to the intricate molecules from simple building blocks. Understanding how...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s10295-015-1636-3

    authors: Chen W,Qi J,Wu P,Wan D,Liu J,Feng X,Deng Z

    更新日期:2016-03-01 00:00:00

  • Identification of metabolites produced from N-phenylpiperazine by Mycobacterium spp.

    abstract::Mycobacterium sp. 7E1B1W and seven other mycobacterial strains known to degrade hydrocarbons were investigated to determine their ability to metabolize the piperazine ring, a substructure found in many drugs. Cultures were grown at 30 degrees C in tryptic soy broth and dosed with 3.1 mM N-phenylpiperazine hydrochlorid...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-006-0189-x

    authors: Adjei MD,Deck J,Heinze TM,Freeman JP,Williams AJ,Sutherland JB

    更新日期:2007-03-01 00:00:00

  • Nitrogen deprivation of microalgae: effect on cell size, cell wall thickness, cell strength, and resistance to mechanical disruption.

    abstract::Nitrogen deprivation (N-deprivation) is a proven strategy for inducing triacylglyceride accumulation in microalgae. However, its effect on the physical properties of cells and subsequently on product recovery processes is relatively unknown. In this study, the effect of N-deprivation on the cell size, cell wall thickn...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-016-1848-1

    authors: Yap BH,Crawford SA,Dagastine RR,Scales PJ,Martin GJ

    更新日期:2016-12-01 00:00:00

  • Cometabolic reduction of bromate by a mixed culture of microorganisms using hydrogen gas in a gas-lift reactor.

    abstract::The discharge of bromate, a suspected carcinogen, will be restricted in the near future. To assess the possibility of biotechnological treatment of bromate-containing wastewaters, the removal of bromate by chlorate-reducing microorganisms was studied. The removal of bromate and chlorate was studied in laboratory gas-l...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-004-0188-8

    authors: van Ginkel CG,Middelhuis BJ,Spijk F,Abma WR

    更新日期:2005-01-01 00:00:00

  • Phytostimulation and biofertilization in wheat by cyanobacteria.

    abstract::Cyanobacteria are commonly used for the phytostimulation and biofertilization of agriculture crops due to their nitrogen-fixing ability. However, the contribution by their phytohormones has been neglected. This study focuses on the screening of rhizospheric and free-living cyanobacteria for in vitro phytohormones prod...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-010-0833-3

    authors: Hussain A,Hasnain S

    更新日期:2011-01-01 00:00:00

  • Rapidly directional biotransformation of tauroursodeoxycholic acid through engineered Escherichia coli.

    abstract::Bear bile powder is a precious medicinal material. It is characterized by high content of tauroursodeoxycholic acid (TUDCA) at a ratio of 1.0-1.5 to taurochenodeoxycholic acid (TCDCA). Here, we reported the biotransformation of tauroursodeoxycholic acid (TUDCA) through Escherichia coli engineered with a two-step mimic...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-017-1935-y

    authors: Shi J,Wang J,Yu L,Yang L,Zhao S,Wang Z

    更新日期:2017-07-01 00:00:00

  • Biosynthesis of plant-specific phenylpropanoids by construction of an artificial biosynthetic pathway in Escherichia coli.

    abstract::Biological synthesis of plant secondary metabolites has attracted increasing attention due to their proven or assumed beneficial properties and health-promoting effects. Phenylpropanoids are the precursors to a range of important plant metabolites such as the secondary metabolites belonging to the flavonoid/stilbenoid...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-011-0954-3

    authors: Choi O,Wu CZ,Kang SY,Ahn JS,Uhm TB,Hong YS

    更新日期:2011-10-01 00:00:00

  • Distribution and diversity of Dipteran-specific cry and cyt genes in native Bacillus thuringiensis strains obtained from different ecosystems of Iran.

    abstract::One hundred and twenty-eight Bacillus thuringiensis isolates from fields of different ecological regions of Iran were collected to study the distribution and diversity of Dipteran-specific cry and cyt genes. The percentage of samples with Bt showed significant differences between different regions and also between dif...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-007-0269-6

    authors: Jouzani GS,Abad AP,Seifinejad A,Marzban R,Kariman K,Maleki B

    更新日期:2008-02-01 00:00:00

  • A recombinant vaccine against hydatidosis: production of the antigen in Escherichia coli.

    abstract::A commercial process was developed for producing a recombinant vaccine against hydatidosis in farm animals. The vaccine antigen consisting of a surface protein of the oncospheres of the hydatid worm (Echinococcus granulosus), was produced as inclusion bodies in Escherichia coli. Fed-batch cultures of E. coli using Ter...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-005-0046-3

    authors: Manderson D,Dempster R,Chisti Y

    更新日期:2006-03-01 00:00:00

  • Biosynthesis and characterization of copolymer poly(3HB-co-3HV) from saponified Jatropha curcas oil by Pseudomonas oleovorans.

    abstract::Polyhydroxyalkanoates (PHAs) are naturally occurring biodegradable polymers with promising application in the formulation of plastic materials. PHAs are produced by numerous bacteria as energy/carbon storage materials from various substrates, including sugars and plant oils. Since these substrates compete as food sour...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-010-0732-7

    authors: Allen AD,Anderson WA,Ayorinde FO,Eribo BE

    更新日期:2010-08-01 00:00:00

  • Sulfide persistence in oil field waters amended with nitrate and acetate.

    abstract::Nitrate amendment is normally an effective method for sulfide control in oil field-produced waters. However, this approach has occasionally failed to prevent sulfide accumulation, despite the presence of active nitrate-reducing bacterial populations. Here, we report our study of bulk chemical transformations in microc...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-009-0639-3

    authors: Hulecki JC,Foght JM,Gray MR,Fedorak PM

    更新日期:2009-12-01 00:00:00

  • Characterisation of aerobically grown non-spore-forming bacteria from paper mill pulps containing recycled fibres.

    abstract::A total of 179 non-spore-forming bacteria aerobically growing on Nutrient Agar, Plate Count Agar or in specific enrichment conditions for salmonella, campylobacteria, listeria, yersinia or staphylococci, were isolated from 16 untreated paper mill pulps. After phenotypical screening the isolates were characterised by a...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-008-0472-0

    authors: Suihko ML,Skyttä E

    更新日期:2009-01-01 00:00:00

  • Use of a stress-minimisation paradigm in high cell density fed-batch Escherichia coli fermentations to optimise recombinant protein production.

    abstract::Production of recombinant proteins is an industrially important technique in the biopharmaceutical sector. Many recombinant proteins are problematic to generate in a soluble form in bacteria as they readily form insoluble inclusion bodies. Recombinant protein solubility can be enhanced by minimising stress imposed on ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-014-1489-1

    authors: Wyre C,Overton TW

    更新日期:2014-09-01 00:00:00

  • Optimising expression of the recombinant fusion protein biopesticide ω-hexatoxin-Hv1a/GNA in Pichia pastoris: sequence modifications and a simple method for the generation of multi-copy strains.

    abstract::Production of recombinant protein bio-insecticides on a commercial scale can only be cost effective if host strains with very high expression levels are available. A recombinant fusion protein containing an arthropod toxin, ω-hexatoxin-Hv1a, (from funnel web spider Hadronyche versuta) linked to snowdrop lectin (Galant...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-014-1466-8

    authors: Pyati P,Fitches E,Gatehouse JA

    更新日期:2014-08-01 00:00:00

  • Enhanced production of amidase from Rhodococcus erythropolis MTCC 1526 by medium optimisation using a statistical experimental design.

    abstract::In the present work, statistical experimental methodology was used to enhance the production of amidase from Rhodococcus erythropolis MTCC 1526. R. erythropolis MTCC 1526 was selected through screening of seven strains of Rhodococcus species. The Placket-Burman screening experiments suggested that sorbitol as carbon s...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-009-0536-9

    authors: Vaidya BK,Mutalik SR,Joshi RM,Nene SN,Kulkarni BD

    更新日期:2009-05-01 00:00:00

  • Clean manufacturing powered by biology: how Amyris has deployed technology and aims to do it better.

    abstract::Amyris is a fermentation product company that leverages synthetic biology and has been bringing novel fermentation products to the market since 2009. Driven by breakthroughs in genome editing, strain construction and testing, analytics, automation, data science, and process development, Amyris has commercialized nine ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-020-02314-3

    authors: Hill P,Benjamin K,Bhattacharjee B,Garcia F,Leng J,Liu CL,Murarka A,Pitera D,Rodriguez Porcel EM,da Silva I,Kraft C

    更新日期:2020-11-01 00:00:00

  • Efficient strategy for maintaining and enhancing the huperzine A production of Shiraia sp. Slf14 through inducer elicitation.

    abstract::Huperzine A (HupA), a naturally occurring lycopodium alkaloid, is a potent, highly specific and reversible inhibitor of acetylcholinesterase and is a potential treatment for Alzheimer's disease. However, isolating HupA from Huperziaceae plants is inefficient; thus, extracting this compound from endophytic fungi may be...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-014-1461-0

    authors: Yan R,Zhang Z,Wang Y,Yang H,Zeng Q,Zhu D

    更新日期:2014-07-01 00:00:00

  • Strain improvement for fermentation and biocatalysis processes by genetic engineering technology.

    abstract::Twenty years ago, the first complete gene cluster encoding the actinorhodin biosynthetic pathway was cloned and characterized. Subsequently, the gene clusters encoding the biosynthetic pathways for many antibiotics were isolated. In the past decade, breakthroughs in technology brought that generation of rationally des...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s10295-004-0131-z

    authors: Chiang SJ

    更新日期:2004-03-01 00:00:00

  • Non-targeted metabolomic reveals the effect of salt stress on global metabolite of halotolerant yeast Candida versatilis and principal component analysis.

    abstract::As one of the major microbes in the soy sauce fermentation, Candida versatilis enriches the flavor and improves the quality of soy sauce. In this study, a combination of five different GC-MS and LC-MS-based metabolome analytical approaches was used to analyze the intracellular, extracellular and whole metabolites of C...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-014-1475-7

    authors: Qi W,Fan ZC,Wang CL,Hou LH,Liu JF,Cao XH

    更新日期:2014-10-01 00:00:00

  • Expression, characterization, and improvement of a newly cloned halohydrin dehalogenase from Agrobacterium tumefaciens and its application in production of epichlorohydrin.

    abstract::A gene encoding halohydrin dehalogenase (HHDH) from Agrobacterium tumefaciens CCTCC M 87071 was cloned and expressed in Escherichia coli. To increase activity and stability of HHDH, 14 amino acid residues around the active site and substrate-binding pocket based on the structural analysis and molecular docking were se...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-014-1443-2

    authors: Liu ZQ,Gao AC,Wang YJ,Zheng YG,Shen YC

    更新日期:2014-07-01 00:00:00

  • Enteric virus contamination of foods through industrial practices: a primer on intervention strategies.

    abstract::Hepatitis A and E viruses, rotaviruses, Norwalk-like caliciviruses, and astroviruses are among the enteric viruses known to cause food- and waterborne illness. These viruses are spread by the fecal-oral route and are a major cause of morbidity and mortality worldwide. Foods may be contaminated at any time pre- or post...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审

    doi:10.1038/sj.jim.7000095

    authors: Richards GP

    更新日期:2001-08-01 00:00:00

  • Madurastatin B3, a rare aziridine derivative from actinomycete Nocardiopsis sp. LS150010 with potent anti-tuberculosis activity.

    abstract::Since the discovery of the first antibiotic, natural products have played an important role in chemistry, biology and medicine. To explore the potential of bioactive compounds from microbes isolated from the southeast of Tibet, China, a crude extract library was constructed and screened against Staphylococcus aureus. ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-017-1908-1

    authors: Zhang X,He H,Ma R,Ji Z,Wei Q,Dai H,Zhang L,Song F

    更新日期:2017-05-01 00:00:00

  • Enhancement of maltose utilisation by Saccharomyces cerevisiae in medium containing fermentable hexoses.

    abstract::Some industrial strains of Saccharomyces cerevisiae are unable to maintain high rates of fermentation during transition from catabolism of hexoses to maltose. This phenomenon, termed 'maltose lag', presents problems for the baking, brewing and distilling industries, which rely on yeast catabolism of mixtures of hexose...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1038/sj.jim.2900672

    authors: Hazell B,Attfield P

    更新日期:1999-06-01 00:00:00

  • Isolation and characterization of a biosurfactant producing strain, Brevibacilis brevis HOB1.

    abstract::Biosurfactant-producing bacteria were isolated from the production water of an oil field. Isolates were screened for biosurfactant production using surface tension test. The highest reduction of surface tension was achieved with a bacterial strain which was identified by 16S rRNA gene sequencing as Brevibacilis brevis...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-008-0403-0

    authors: Haddad NI,Wang J,Mu B

    更新日期:2008-12-01 00:00:00

  • Red recombinase assisted gene replacement in Klebsiella pneumoniae.

    abstract::The Red recombinase system, the most convenient genetic tool applied in Escherichia coli and other bacteria, was introduced for gene replacement in Klebsiella pneumoniae. The novel K. pneumoniae gene replacement system comprised the Red and FLP recombinases expression vector pDK6-red and pDK6-flp, and linear DNA fragm...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-012-1117-x

    authors: Wei D,Wang M,Shi J,Hao J

    更新日期:2012-08-01 00:00:00

  • Ram horn peptone as a source of citric acid production by Aspergillus niger, with a process.

    abstract::The present study deals with the production of citric acid from a ram horn peptone (RHP) by Aspergillus niger NRRL 330. A medium from RHP and a control medium (CM) were compared for citric acid production using A. niger in a batch culture. For this purpose, first, RHP was produced. Ram horns were hydrolyzed by treatme...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-004-0147-4

    authors: Kurbanoglu EB,Kurbanoglu NI

    更新日期:2004-07-01 00:00:00