The application of multi-parameter flow cytometry to the study of recombinant Escherichia coli batch fermentation processes.

Abstract:

:Multi-parameter flow cytometric techniques coupled with dual colour fluorescent staining were used to study the physical and metabolic consequences of inclusion body formation in batch cultures of the recombinant Escherichia coli strain MSD3735. This strain contains a plasmid coding for the isopropylthiogalactopyranoside-inducible model eukaryotic protein AP50. It is known that the synthesis of foreign proteins at high concentrations can exert a severe metabolic stress on the host cell and that morphological changes can occur. In this work, using various points of induction, it was shown that inclusion body formation is followed immediately by measurable changes in the characteristic intrinsic light scatter patterns for the individual cell (forward scatter, 90 degrees side scatter) and a concomitant progressive change in the individual cell physiological state with respect to both cytoplasmic membrane polarisation and permeability. This work establishes flow cytometry as a potentially valuable tool for monitoring recombinant fermentation processes, providing important information for scale-up. Further, we discuss the possibility of optimising inclusion body formation by manipulating the fermentation conditions based on these rapid "real-time" measurements.

authors

Lewis G,Taylor IW,Nienow AW,Hewitt CJ

doi

10.1007/s10295-004-0151-8

subject

Has Abstract

pub_date

2004-08-01 00:00:00

pages

311-22

issue

7

eissn

1367-5435

issn

1476-5535

journal_volume

31

pub_type

杂志文章
  • Heterotrophic nitrogen removal by Providencia rettgeri strain YL.

    abstract::Providencia rettgeri strain YL was found to be efficient in heterotrophic nitrogen removal under aerobic conditions. Maximum removal of NH(4) (+)-N occurred under the conditions of pH 7 and supplemented with glucose as the carbon source. Inorganic ions such as Mg(2+), Mn(2+), and Zn(2+) largely influenced the growth a...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-010-0708-7

    authors: Zhao B,He YL,Huang J,Taylor S,Hughes J

    更新日期:2010-06-01 00:00:00

  • Chemical improvement of chitosan-modified beads for the immobilization of Enterococcus faecium DBFIQ E36 L-arabinose isomerase through multipoint covalent attachment approach.

    abstract::D-tagatose is produced from D-galactose by the enzyme L-arabinose isomerase (L-AI) in a commercially viable bioprocess. An active and stable biocatalyst was obtained by modifying chitosan gel structure through reaction with TNBS, D-fructose or DMF, among others. This led to a significant improvement in L-AI immobiliza...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-015-1662-1

    authors: Manzo RM,de Sousa M,Fenoglio CL,Gonçalves LR,Mammarella EJ

    更新日期:2015-10-01 00:00:00

  • Discovery of a new diol-containing polyketide by heterologous expression of a silent biosynthetic gene cluster from Streptomyces lavendulae FRI-5.

    abstract::The genome of streptomycetes has the ability to produce many novel and potentially useful bioactive compounds, but most of which are not produced under standard laboratory cultivation conditions and are referred to as silent/cryptic secondary metabolites. Streptomyces lavendulae FRI-5 produces several types of bioacti...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-017-1997-x

    authors: Pait IGU,Kitani S,Roslan FW,Ulanova D,Arai M,Ikeda H,Nihira T

    更新日期:2018-02-01 00:00:00

  • DNA assembly techniques for next-generation combinatorial biosynthesis of natural products.

    abstract::Natural product scaffolds remain important leads for pharmaceutical development. However, transforming a natural product into a drug entity often requires derivatization to enhance the compound's therapeutic properties. A powerful method by which to perform this derivatization is combinatorial biosynthesis, the manipu...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s10295-013-1358-3

    authors: Cobb RE,Ning JC,Zhao H

    更新日期:2014-02-01 00:00:00

  • Biodegradation of aromatic compounds under mixed oxygen/denitrifying conditions: a review.

    abstract::Bioremediation of aromatic hydrocarbons in groundwater and sediments is often limited by dissolved oxygen. Many aromatic hydrocarbons degrade very slowly or not at all under anaerobic conditions. Nitrate is a good alternative electron acceptor to oxygen, and denitrifying bacteria are commonly found in the subsurface a...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审

    doi:10.1038/sj.jim.2900288

    authors: Wilson LP,Bouwer EJ

    更新日期:1997-02-01 00:00:00

  • Fabclavine biosynthesis in X. szentirmaii: shortened derivatives and characterization of the thioester reductase FclG and the condensation domain-like protein FclL.

    abstract::Fabclavines, unusual peptide-polyketide-polyamine hybrids, show broad-spectrum bioactivity against a variety of different organism like Gram-positive and -negative bacteria, fungi and protozoa. We elucidated the biosynthesis of these NRPS-PKS hybrids in Xenorhabdus szentirmaii by deletion of most genes encoded in the ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-018-02124-8

    authors: Wenski SL,Kolbert D,Grammbitter GLC,Bode HB

    更新日期:2019-03-01 00:00:00

  • In silico aided metabolic engineering of Klebsiella oxytoca and fermentation optimization for enhanced 2,3-butanediol production.

    abstract::Klebsiella oxytoca naturally produces a large amount of 2,3-butanediol (2,3-BD), a promising bulk chemical with wide industrial applications, along with various byproducts. In this study, the in silico gene knockout simulation of K. oxytoca was carried out for 2,3-BD overproduction by inhibiting the formation of bypro...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-013-1298-y

    authors: Park JM,Song H,Lee HJ,Seung D

    更新日期:2013-09-01 00:00:00

  • Sequential improvement of rimocidin production in Streptomyces rimosus M527 by introduction of cumulative drug-resistance mutations.

    abstract::Rimocidin is a polyene macrolide that exhibits a strong inhibitory activity against a broad range of plant-pathogenic fungi. In this study, fermentation optimization and ribosome engineering technology were employed to enhance rimocidin production in Streptomyces rimosus M527. After the optimization of fermentation, r...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-019-02146-w

    authors: Zhao Y,Song Z,Ma Z,Bechthold A,Yu X

    更新日期:2019-05-01 00:00:00

  • Detection and partial characterization of a bacteriocin produced by Carnobacterium piscicola 213.

    abstract::BLIS 213, is a bacteriocin-like inhibitory substance produced by Carnobacterium piscicola 213. It is active against Carnobacterium, Enterococcus and Listeria spp. No activity was observed against tested Lactobacillus, Lactococcus, Leuconostoc and Pediococcus strains, nor against Gram-negative bacteria. The BLIS 213 ac...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1038/sj.jim.2900412

    authors: Khouiti Z,Simon JP

    更新日期:1997-07-01 00:00:00

  • Enhanced poly-γ-L-diaminobutanoic acid production in Bacillus pumilus by combining genome shuffling with multiple antibiotic-resistance.

    abstract::A breeding approach combining genome shuffling with multiple antibiotic-resistance including gentamicin, rifampin and lincomycin, was developed in this research to improve the poly-γ-L-diaminobutanoic acid (γ-PAB) production in Bacillus pumilus LS-1. By this unique strategy, recombinants from the third round of genome...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-020-02315-2

    authors: Li S,Wang L,Wang N

    更新日期:2020-12-01 00:00:00

  • Purification and characterization of an organic-solvent-tolerant cellulase from a halotolerant isolate, Bacillus sp. L1.

    abstract::A halotolerant isolate Bacillus sp. L1 producing extracellular cellulase was isolated from Yuncheng, China. Production of the enzyme started from mid-exponential phase of bacterial growth and reached a maximum level during the post-stationary phase. The cellulase was purified to homogeneity with molecular mass of 45 k...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-012-1120-2

    authors: Li X,Yu HY

    更新日期:2012-08-01 00:00:00

  • Strain development of Streptomyces sp. for tacrolimus production using sequential adaptation.

    abstract::An immunosuppressant tacrolimus-producing strain of Streptomyces sp. TST8 was isolated and developed by the TS corporation in Korea using the sequential adaptation of media containing tacrolimus (600-1600 mg/l). The aim of the tacrolimus sequential adaptation protocol was to select those cells with tacrolimus resistan...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-009-0634-8

    authors: Jung S,Moon S,Lee K,Park YJ,Yoon S,Yoo YJ

    更新日期:2009-12-01 00:00:00

  • Salmonella spp. are affected by different levels of water activity in closed microcosms.

    abstract::Controlling water activity (a(w)) can significantly impact the growth of Salmonella in poultry litter and manure - a phenomenon that was studied quantitatively using two common serotypes of Salmonella. The quantitative effect of changes in levels of a(w) on Salmonella populations was determined using inoculated, frost...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1038/sj.jim.7000116

    authors: Eriksson de Rezende CL,Mallinson ET,Gupte A,Joseph SW

    更新日期:2001-04-01 00:00:00

  • Characterization and comparative analysis of toxin-antitoxin systems in Acetobacter pasteurianus.

    abstract::Bacterial toxin-antitoxin (TA) systems play important roles in diverse cellular regulatory processes. Here, we characterize three putative type II TA candidates from Acetobacter pasteurianus and investigate the profile of type II TA systems in the genus Acetobacter. Based on the gene structure and activity detection, ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-019-02144-y

    authors: Xia K,Bao H,Zhang F,Linhardt RJ,Liang X

    更新日期:2019-06-01 00:00:00

  • Biological deinking of inkjet-printed paper using Vibrio alginolyticus and its enzymes.

    abstract::Recycling of office waste paper (photocopy, inkjet, and laser prints) is a major problem due to difficulty in removal of nonimpact ink. Biological deinking of office waste paper is reported using several microorganisms and their enzymes. We report here deinking and decolorization of the dislodged ink particles from in...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-005-0017-8

    authors: Mohandass C,Raghukumar C

    更新日期:2005-09-01 00:00:00

  • The mycinose-biosynthetic genes of Streptomyces fradiae, producer of tylosin.

    abstract::The tylE-J region of the tylosin-biosynthetic gene cluster of Streptomyces fradiae contains six open reading frames. The products of tylJ and tylD are nucleoside diphospho (NDP)-deoxyhexose 3-epimerase and NDP-deoxyhexose 4-ketoreductase, respectively, involved in the synthesis of NDP-6-deoxyallose from NDP-4-keto, 6-...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1038/sj.jim.2900707

    authors: Bate N,Cundliffe E

    更新日期:1999-08-01 00:00:00

  • Functional expression of a single-chain antibody fragment against human epidermal growth factor receptor 2 (HER2) in Escherichia coli.

    abstract::The human epidermal growth factor receptor (HER) family plays an important role in cell growth and signaling and alteration of its function has been demonstrated in many different kinds of cancer. Receptor dimerization is necessary for the HER signal transduction pathway and tyrosine kinase activity. Recently, several...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-014-1437-0

    authors: Akbari V,Mir Mohammad Sadeghi H,Jafrian-Dehkordi A,Abedi D,Chou CP

    更新日期:2014-06-01 00:00:00

  • Analysis of respiratory activity and carbon usage of a mutant of Azotobacter vinelandii impaired in poly-β-hydroxybutyrate synthesis.

    abstract::In this study, the respiratory activity and carbon usage of the mutant strain of A. vinelandii AT6, impaired in poly-β-hydroxybutyrate (PHB) production, and their relationship with the synthesis of alginate were evaluated. The alginate yield and the specific oxygen uptake rate were higher (2.5-fold and 62 %, respectiv...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-016-1774-2

    authors: Jiménez L,Castillo T,Flores C,Segura D,Galindo E,Peña C

    更新日期:2016-08-01 00:00:00

  • Adaptation of the xylose fermenting yeast Saccharomyces cerevisiae F12 for improving ethanol production in different fed-batch SSF processes.

    abstract::An efficient fermenting microorganism for bioethanol production from lignocellulose is highly tolerant to the inhibitors released during pretreatment and is able to ferment efficiently both glucose and xylose. In this study, directed evolution was employed to improve the xylose fermenting Saccharomyces cerevisiae F12 ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-010-0768-8

    authors: Tomás-Pejó E,Ballesteros M,Oliva JM,Olsson L

    更新日期:2010-11-01 00:00:00

  • Distribution and diversity of Dipteran-specific cry and cyt genes in native Bacillus thuringiensis strains obtained from different ecosystems of Iran.

    abstract::One hundred and twenty-eight Bacillus thuringiensis isolates from fields of different ecological regions of Iran were collected to study the distribution and diversity of Dipteran-specific cry and cyt genes. The percentage of samples with Bt showed significant differences between different regions and also between dif...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-007-0269-6

    authors: Jouzani GS,Abad AP,Seifinejad A,Marzban R,Kariman K,Maleki B

    更新日期:2008-02-01 00:00:00

  • Clean manufacturing powered by biology: how Amyris has deployed technology and aims to do it better.

    abstract::Amyris is a fermentation product company that leverages synthetic biology and has been bringing novel fermentation products to the market since 2009. Driven by breakthroughs in genome editing, strain construction and testing, analytics, automation, data science, and process development, Amyris has commercialized nine ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-020-02314-3

    authors: Hill P,Benjamin K,Bhattacharjee B,Garcia F,Leng J,Liu CL,Murarka A,Pitera D,Rodriguez Porcel EM,da Silva I,Kraft C

    更新日期:2020-11-01 00:00:00

  • Growth of Trametes versicolor on phenol.

    abstract::Trametes versicolor 1 was shown to grow on phenol as its sole carbon and energy source. The culture growth and degradation ability dependence on culture medium pH value was observed. The optimal pH value of a liquid Czapek salt medium was 6.5. The investigated strain utilized completely 0.5 g/l phenol in 6 days. The d...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-008-0412-z

    authors: Yemendzhiev H,Gerginova M,Krastanov A,Stoilova I,Alexieva Z

    更新日期:2008-11-01 00:00:00

  • Phytoremediation of toxic trace elements in soil and water.

    abstract::Toxic heavy metals and metalloids, such as cadmium, lead, mercury, arsenic, and selenium, are constantly released into the environment. There is an urgent need to develop low-cost, effective, and sustainable methods for their removal or detoxification. Plant-based approaches, such as phytoremediation, are relatively i...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s10295-005-0227-0

    authors: LeDuc DL,Terry N

    更新日期:2005-12-01 00:00:00

  • Isolation and characterization of a biosurfactant producing strain, Brevibacilis brevis HOB1.

    abstract::Biosurfactant-producing bacteria were isolated from the production water of an oil field. Isolates were screened for biosurfactant production using surface tension test. The highest reduction of surface tension was achieved with a bacterial strain which was identified by 16S rRNA gene sequencing as Brevibacilis brevis...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-008-0403-0

    authors: Haddad NI,Wang J,Mu B

    更新日期:2008-12-01 00:00:00

  • Xylanase II from Trichoderma reesei QM 9414: conformational and catalytic stability to Chaotropes, Trifluoroethanol, and pH changes.

    abstract::Xylanase II, a key enzyme in the hydrolysis of xylan, was purified from cultures of Trichoderma reesei QM 9414 (anamorph of Hypocrea jecorina) grown on wheat straw as a carbon source. Xylanase treated with increasing guanidinium hydrochloride concentrations was denatured in a cooperative way regarding secondary and te...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-010-0836-0

    authors: López G,Bañares-Hidalgo A,Estrada P

    更新日期:2011-01-01 00:00:00

  • The behavior of key enzymes of xylose metabolism on the xylitol production by Candida guilliermondii grown in hemicellulosic hydrolysate.

    abstract::A variety of raw materials have been used in fermentation process. This study shows the use of rice straw hemicellulosic hydrolysate, as the only source of nutrient, to produce high added-value products. In the present work, the activity of the enzymes xylose reductase (XR); xylitol dehydrogenase (XD); and glucose-6-p...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-008-0475-x

    authors: Gurpilhares DB,Hasmann FA,Pessoa A Jr,Roberto IC

    更新日期:2009-01-01 00:00:00

  • Moderate expression of the transcriptional regulator ALsR enhances acetoin production by Bacillus subtilis.

    abstract::Acetoin, a major extracellular catabolic product of Bacillus subtilis cultured on glucose, is widely used to add flavor to food and also serves as a precursor for chemical synthesis. The biosynthesis of acetoin from pyruvate requires the enzymes α-acetolactate synthase (ALS) and α-acetolactate decarboxylase (ALDC), bo...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-013-1303-5

    authors: Zhang X,Zhang R,Bao T,Yang T,Xu M,Li H,Xu Z,Rao Z

    更新日期:2013-09-01 00:00:00

  • Degradation of substituted naphthalenesulfonic acids by Sphingomonas xenophaga BN6.

    abstract::Sphingomonas xenophaga BN6 was isolated from the river Elbe as a member of a multispecies bacterial culture which mineralized 6-aminonaphthalene-2-sulfonate. Pure cultures of strain BN6 converted a wide range of amino- and hydroxynaphthalene-2-sulfonates via a catabolic pathway similar to that described for the metabo...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1038/sj.jim.2900725

    authors: Stolz A

    更新日期:1999-10-01 00:00:00

  • Electrochemical behavior of the 316L steel type in a marine culture of microalgae (Porphyridium purpureum) under the 12/12 h photoperiod and effect of different working electrode exposure conditions on the biofilm-metal interface.

    abstract::The industrial crops of microalgae use processes calling upon the presence of parts of metal nature such as steel 316L type. The goal of this study is to test the electrochemical behavior of this material in a marine culture of microalgae. Porphyridium purpureum was used under a photoperiod of alternation darkness/lig...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-011-0986-8

    authors: Djemai-Zoghlache Y,Isambert A,Belhaneche-Bensemra N

    更新日期:2011-12-01 00:00:00

  • Microarray analysis of p-anisaldehyde-induced transcriptome of Saccharomyces cerevisiae.

    abstract::p-Anisaldehyde (4-methoxybenzaldehyde), an extract from Pimpinella anisum L. seeds, is a potential novel preservative. To reveal the possible action mechanism of p-anisaldehyde against microorganisms, yeast-based commercial oligonucleotide microarrays were used to analyze the genome-wide transcriptional changes in res...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s10295-009-0676-y

    authors: Yu L,Guo N,Yang Y,Wu X,Meng R,Fan J,Ge F,Wang X,Liu J,Deng X

    更新日期:2010-03-01 00:00:00