Abstract:
:Natural product scaffolds remain important leads for pharmaceutical development. However, transforming a natural product into a drug entity often requires derivatization to enhance the compound's therapeutic properties. A powerful method by which to perform this derivatization is combinatorial biosynthesis, the manipulation of the genes in the corresponding pathway to divert synthesis towards novel derivatives. While these manipulations have traditionally been carried out via restriction digestion/ligation-based cloning, the shortcomings of such techniques limit their throughput and thus the scope of corresponding combinatorial biosynthesis experiments. In the burgeoning field of synthetic biology, the demand for facile DNA assembly techniques has promoted the development of a host of novel DNA assembly strategies. Here we describe the advantages of these recently developed tools for rapid, efficient synthesis of large DNA constructs. We also discuss their potential to facilitate the simultaneous assembly of complete libraries of natural product biosynthetic pathways, ushering in the next generation of combinatorial biosynthesis.
journal_name
J Ind Microbiol Biotechnoljournal_title
Journal of industrial microbiology & biotechnologyauthors
Cobb RE,Ning JC,Zhao Hdoi
10.1007/s10295-013-1358-3subject
Has Abstractpub_date
2014-02-01 00:00:00pages
469-77issue
2eissn
1367-5435issn
1476-5535journal_volume
41pub_type
杂志文章,评审abstract::p-Anisaldehyde (4-methoxybenzaldehyde), an extract from Pimpinella anisum L. seeds, is a potential novel preservative. To reveal the possible action mechanism of p-anisaldehyde against microorganisms, yeast-based commercial oligonucleotide microarrays were used to analyze the genome-wide transcriptional changes in res...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-009-0676-y
更新日期:2010-03-01 00:00:00
abstract::A 2 M sodium acetate buffer at pH 4.2 was tried to simplify the step of pH adjustment in a laboratory dry-grind procedure. Ethanol yields or conversion efficiencies of 18 sorghum hybrids improved significantly with 2.0-5.9% (3.9% on average) of relative increases when the method of pH adjustment changed from tradition...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-008-0474-y
更新日期:2009-01-01 00:00:00
abstract::A method was developed to quantify the performance of microorganisms involved in different digestion levels in biogas plants. The test system was based on the addition of butyrate (BCON), ethanol (ECON), acetate (ACON) or propionate (PCON) to biogas sludge samples and the subsequent analysis of CH4 formation in compar...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-016-1894-8
更新日期:2017-03-01 00:00:00
abstract::Penicillium strain isolated from citrus fruit was found to produce thermostable polygalacturonases. Optimization of process parameters resulted in high levels of enzyme production after 3 days of incubation at a pH of 5.0 at 30 degrees C in the presence of 1% pectin. The optimum temperature for enzyme activity was 60 ...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-008-0375-0
更新日期:2008-09-01 00:00:00
abstract::Two cultures, a yeast ( Rhodorula rubra GED8) and a yogurt starter ( Lactobacillus bulgaricus 2-11+ Streptococcus thermophilus 15HA), were selected for associated growth in whey ultrafiltrate (WU) and active synthesis of carotenoids. In associated cultivation with the yogurt culture L bulgaricus 2-11+S. thermophilus 1...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-004-0122-0
更新日期:2004-03-01 00:00:00
abstract::Bacterial contamination of commercial fermentation cultures is a common and costly problem to the fuel ethanol industry. Antimicrobials such as virginiamycin (VIR) and penicillin (PEN) are frequently used to control contamination but there are little data available on the susceptibility of bacterial contaminants to th...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-007-0250-4
更新日期:2007-11-01 00:00:00
abstract::Physiological studies and biotechnology applications of Geobacter species have been limited by a lack of genetic tools. Therefore, potential additional molecular strategies for controlling metabolism were explored. When the gene for citrate synthase, or acetyl-CoA transferase, was placed under the control of a LacI/IP...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-016-1836-5
更新日期:2016-11-01 00:00:00
abstract::Based on published results and investigations done for this study, chemotaxonomic characteristics of all validly described species of the genus Sphingomonas, as well as unnamed strains of this genus and related genera such as Rhizomonas and Blastomonas, are presented. All representatives of this group, here designated...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1038/sj.jim.2900745
更新日期:1999-10-01 00:00:00
abstract::L-Phenylalanine ammonia-lyase (PAL, EC 4.3.1.25) from Rhodosporidium toruloides was utilized to remove L-phenylalanine (L-Phe) from different commercial protein hydrolysates. A casein acid hydrolysate (CAH, L-Phe ~2.28 %) was employed as a model substrate. t-Cinnamic acid resulting from deamination of L-Phe was extrac...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-015-1664-z
更新日期:2015-10-01 00:00:00
abstract::Many sludge reduction processes have been studied for the minimization of sludge production in biological wastewater treatment. The investigations on most of these processes have monitored the increase of the soluble chemical oxygen demand, the sludge mass reduction, or the decrease of the floc size, but little inform...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-008-0423-9
更新日期:2008-11-01 00:00:00
abstract::Natural products (NPs) produced by bacteria and fungi are often used as therapeutic agents due to their complex structures and wide range of bioactivities. Enzymes that build NPs are encoded by co-localized biosynthetic gene clusters (BGCs), and genome sequencing has recently revealed that many BGCs are "silent" under...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s10295-019-02198-y
更新日期:2019-10-01 00:00:00
abstract::A novel lipolytic enzyme was isolated from a metagenomic library after demonstration of lipolytic activity on an LB agar plate containing 1% (w/v) tributyrin. A novel esterase gene (estIM1), encoding a lipolytic enzyme (EstIM1), was cloned using a shotgun method from a pFosEstIM1 clone of the metagenomic library, and ...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-011-1080-y
更新日期:2012-05-01 00:00:00
abstract::The objective of this study was to demonstrate the feasibility of novel, Horizontal Flow Biofilm Reactor (HFBR) technology for the treatment of ammonia (NH3)-contaminated airstreams. Three laboratory-scale HFBRs were used for remediation of an NH3-containing airstream at 10 °C during a 90-d trial to test the efficacy ...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-016-1740-z
更新日期:2016-05-01 00:00:00
abstract::The antifungal-producing potential of actinomycete populations from the rhizosphere of low-altitude sagebrush, Artemisia tridentata, has been examined. In a continued investigation of new sources of antifungal-producing microorganisms, this study examined the antifungal-producing potential of actinomycetes from the rh...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-005-0007-x
更新日期:2005-08-01 00:00:00
abstract::Natural product discovery in the microbial world has historically been biased toward aerobes. Recent in silico analysis demonstrates that genomes of anaerobes encode unexpected biosynthetic potential for natural products, however, chemical data on natural products from the anaerobic world are extremely limited. Here, ...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s10295-018-2086-5
更新日期:2019-03-01 00:00:00
abstract::The effect of pH, aeration and mixing on the growth and production of carbonyl reductase by Candida viswanathii was investigated in a 6.6-l fermentor. Controlling the pH at 8.0 had a very significant effect on the enzyme production. Aeration and agitation influenced the dissolved oxygen concentration which in turn aff...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-007-0280-y
更新日期:2008-03-01 00:00:00
abstract::BLIS 213, is a bacteriocin-like inhibitory substance produced by Carnobacterium piscicola 213. It is active against Carnobacterium, Enterococcus and Listeria spp. No activity was observed against tested Lactobacillus, Lactococcus, Leuconostoc and Pediococcus strains, nor against Gram-negative bacteria. The BLIS 213 ac...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1038/sj.jim.2900412
更新日期:1997-07-01 00:00:00
abstract::Pseudomonas aeruginosa UW-1 produced 17-24 g L-1 rhamnolipid in vegetable oil-containing media in shake flask cultures in 13 days. In time course studies of growth and rhamnolipid production in a salts medium containing 6% canola oil, total bacterial count reached 2.6 x 10(10) CFU ml-1 after 48 h and a maximum rhamnol...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1038/sj.jim.2900450
更新日期:1997-10-01 00:00:00
abstract::Using a simple method to introduce genetic modifications into the chromosome of naturally nontransformable Bacillus, a set of marker-free inosine-producing and 5-aminoimidazole-4-carboxamide (AICA) ribonucleoside-producing Bacillus amyloliquefaciens strains has been constructed. These strains differ in expression leve...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-010-0829-z
更新日期:2011-01-01 00:00:00
abstract::The ability of bacteria to attach to surfaces and develop into a biofilm has been of considerable interest to many groups in numerous industries, including the medical and food industry. However, little is understood in the critical initial step seen in all biofilm development, the initial bacterial cell attachment to...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s10295-007-0234-4
更新日期:2007-09-01 00:00:00
abstract::There have been numerous developments in ethanol fermentation technology since the beginning of the new millennium as ethanol has become an immediate viable alternative to fast-depleting crude reserves as well as increasing concerns over environmental pollution. Nowadays, although most research efforts are focused on ...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s10295-011-0999-3
更新日期:2011-09-01 00:00:00
abstract::Fungi are well known for their vast diversity of secondary metabolites that include many life-saving drugs and highly toxic mycotoxins. In general, fungal cultures producing such metabolites are immune to their toxic effects. However, some are known to produce self-toxic compounds that can pose production optimization...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-009-0678-9
更新日期:2010-04-01 00:00:00
abstract::Given the recent increases in natural gas reserves and associated drawbacks of current gas-to-liquids technologies, the development of a bioconversion process to directly convert methane to liquid fuels would generate considerable industrial interest. Several clades of anaerobic methanotrophic archaea (ANME) are capab...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s10295-014-1548-7
更新日期:2015-03-01 00:00:00
abstract::Batch cultures of Ralstonia eutropha in chemically defined media with acetic acid (HAc) as the sole carbon source were conducted to investigate acetate utilization, formation of poly(3-hydroxybutyrate) (PHB) and growth of active biomass (ABM) under different carbon to nitrogen (C/N) weight ratios. The specific acetate...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1038/sj.jim.7000097
更新日期:2001-03-01 00:00:00
abstract::Production of recombinant proteins is an industrially important technique in the biopharmaceutical sector. Many recombinant proteins are problematic to generate in a soluble form in bacteria as they readily form insoluble inclusion bodies. Recombinant protein solubility can be enhanced by minimising stress imposed on ...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-014-1489-1
更新日期:2014-09-01 00:00:00
abstract::Glycerol, cassava wastewater (CW), waste cooking oil and CW with waste frying oils were evaluated as alternative low-cost carbon substrates for the production of rhamnolipids and polyhydroxyalkanoates (PHAs) by various Pseudomonas aeruginosa strains. The polymers and surfactants produced were characterized by gas chro...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-009-0590-3
更新日期:2009-08-01 00:00:00
abstract::Amyris is a fermentation product company that leverages synthetic biology and has been bringing novel fermentation products to the market since 2009. Driven by breakthroughs in genome editing, strain construction and testing, analytics, automation, data science, and process development, Amyris has commercialized nine ...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-020-02314-3
更新日期:2020-11-01 00:00:00
abstract::An acid proteinase from Monascus purpureus No. 3403, MpuAP, was previously purified and some characterized in our laboratory (Agric Biol Chem 48:1637-1639, 1984). However, further information about this enzyme is lacking. In this study, we investigated MpuAP's comprehensive substrate specificity, storage stability, an...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-010-0933-0
更新日期:2011-09-01 00:00:00
abstract::Mycobacterium sp. 7E1B1W and seven other mycobacterial strains known to degrade hydrocarbons were investigated to determine their ability to metabolize the piperazine ring, a substructure found in many drugs. Cultures were grown at 30 degrees C in tryptic soy broth and dosed with 3.1 mM N-phenylpiperazine hydrochlorid...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-006-0189-x
更新日期:2007-03-01 00:00:00
abstract::A strain of Bacillus subtilis was examined for antifungal activity against phytopathogenic and wood-surface contaminant fungi. The bacterium was grown in five culture media with different incubation times in order to study cell development, sporulation, and the production of metabolites with antifungal activity. The a...
journal_title:Journal of industrial microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s10295-004-0133-x
更新日期:2004-06-01 00:00:00