Abstract：
:Marine sediments from Newfoundland, Canada were explored for biotechnologically promising Actinobacteria using culture-independent and culture-dependent approaches. Culture-independent pyrosequencing analyses uncovered significant actinobacterial diversity (H'-2.45 to 3.76), although the taxonomic diversity of biotechnologically important actinomycetes could not be fully elucidated due to limited sampling depth. Assessment of culturable actinomycete diversity resulted in the isolation of 360 actinomycetes representing 59 operational taxonomic units, the majority of which (94 %) were Streptomyces. The biotechnological potential of actinomycetes from NL sediments was assessed by bioactivity and metabolomics-based screening of 32 representative isolates. Bioactivity was exhibited by 41 % of isolates, while 11 % exhibited unique chemical signatures in metabolomics screening. Chemical analysis of two isolates resulted in the isolation of the cytotoxic metabolite 1-isopentadecanoyl-3β-D-glucopyranosyl-X-glycerol from Actinoalloteichus sp. 2L868 and sungsanpin from Streptomyces sp. 8LB7. These results demonstrate the potential for the discovery of novel bioactive metabolites from actinomycetes isolated from Atlantic Canadian marine sediments.
journal_name
J Ind Microbiol Biotechnoljournal_title
Journal of industrial microbiology & biotechnologyauthors
Duncan KR,Haltli B,Gill KA,Correa H,Berrué F,Kerr RGdoi
10.1007/s10295-014-1529-xsubject
Has Abstractpub_date
2015-01-01 00:00:00pages
57-72issue
1eissn
1367-5435issn
1476-5535journal_volume
42pub_type
杂志文章abstract：:Contamination of Lactobacillus sp. in the fermentation broth of bioethanol production decreases ethanol production efficiency. Although the addition of lactate to the broth can effectively inhibit the growth of Lactobacillus sp., it also greatly reduces the fermentation ability of Saccharomyces cerevisiae. To overcome...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-008-0390-1
更新日期：2008-10-01 00:00:00
abstract：:Industrial applications for lactate, such as the production of chemicals, has led to interest in producing this organic acid by metabolically engineered a yeast such as Saccharomyces cerevisiae, which is more acid tolerant than lactic acid bacteria. This paper deals with lactate production by S. cerevisiae K1-LDH, in ...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-004-0138-5
更新日期：2004-06-01 00:00:00
abstract：:The polyketide aglycone, tylactone (protylonolide), does not normally accumulate during tylosin production in Streptomyces fradiae, suggesting that the capacity of the organism to glycosylate tylactone exceeds the capacity for polyketide synthesis. Consistent with this model, tylosin yields were significantly increase...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1038/sj.jim.7000160
更新日期：2001-07-01 00:00:00
abstract：:A total of 179 non-spore-forming bacteria aerobically growing on Nutrient Agar, Plate Count Agar or in specific enrichment conditions for salmonella, campylobacteria, listeria, yersinia or staphylococci, were isolated from 16 untreated paper mill pulps. After phenotypical screening the isolates were characterised by a...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-008-0472-0
更新日期：2009-01-01 00:00:00
abstract：:The ability of bacteria to attach to surfaces and develop into a biofilm has been of considerable interest to many groups in numerous industries, including the medical and food industry. However, little is understood in the critical initial step seen in all biofilm development, the initial bacterial cell attachment to...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s10295-007-0234-4
更新日期：2007-09-01 00:00:00
abstract：:Bacterial contamination is an ongoing problem for commercial fuel ethanol production facilities. Both chronic and acute infections are of concern, due to the fact that bacteria compete with the ethanol-producing yeast for sugar substrates and micronutrients. Lactic acid levels often rise during bouts of contamination,...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-004-0159-0
更新日期：2004-10-01 00:00:00
abstract：:A spectrofluorometric assay was developed for quantification of bacterial biofilms grown on a microtiter plate. The method involved staining biofilms formed by gram-negative and gram-positive bacteria with wheat germ agglutinin-Alexa Fluor 488 conjugate, which selectively binds to N-acetylglucosamine residues in biofi...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-006-0086-3
更新日期：2007-01-01 00:00:00
abstract：:Corynebacterium glutamicum SNK 118 was metabolically engineered with improved L-arginine titer. Considering the crucial role of NADPH level in L-arginine production, pntAB (membrane-bound transhydrogenase) and ppnK (NAD+ kinase) were co-expressed to increase the intracellular NADPH pool. Expression of pntAB exhibited ...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-018-2103-8
更新日期：2019-01-01 00:00:00
abstract：:Using a simple method to introduce genetic modifications into the chromosome of naturally nontransformable Bacillus, a set of marker-free inosine-producing and 5-aminoimidazole-4-carboxamide (AICA) ribonucleoside-producing Bacillus amyloliquefaciens strains has been constructed. These strains differ in expression leve...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-010-0829-z
更新日期：2011-01-01 00:00:00
abstract：:Streptomyces strain LL-P018 produces the phaeochromycins, novel anti-inflammatory polyketides. This organism was identified as a strain of Streptomyces phaeochromogenes by physiological and genetic taxonomic analysis. In order to gain greater taxonomic perspective, LL-P018 was compared to related strains from major cu...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-008-0367-0
更新日期：2008-09-01 00:00:00
abstract：:Solar salterns can be modeled as giant outdoor chemostats, much like a series of dams on a slow-moving river. Microorganisms and their products play an essential, but sometimes uncharacterized, role in salt production in these ponds, from seawater salinity up through NaCl saturation. They may physically affect the eva...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1038/sj/jim/7000173
更新日期：2002-01-01 00:00:00
abstract：:The type strains of the anamorph Phaffia rhodozyma (CBS 5905) and the teleomorph Xanthophyllomyces dendrorhous (VKM Y-2786) were analyzed by nucleotide sequence analysis and compared to the sequences found in three additional strains (ATCC 24228, ATCC 24230 and CBS 6938). The results of ribosomal DNA Internal transcri...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1038/sj.jim.2900681
更新日期：1999-07-01 00:00:00
abstract：:Production of recombinant proteins is an industrially important technique in the biopharmaceutical sector. Many recombinant proteins are problematic to generate in a soluble form in bacteria as they readily form insoluble inclusion bodies. Recombinant protein solubility can be enhanced by minimising stress imposed on ...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-014-1489-1
更新日期：2014-09-01 00:00:00
abstract：:The overall objective of this study is to evaluate the morphological [scanning electron microscopy (SEM)], physicochemical [differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), chemical composition analysis, Fourier-transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR)], and bi...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-011-1027-3
更新日期：2012-02-01 00:00:00
abstract：:Traditional synthesis of biodiesel competes with food sources and has limitations with storage, particularly due to limited oxidative stability. Microbial synthesis of lipids provides a platform to produce renewable fuel with improved properties from various renewable carbon sources. Specifically, biodiesel properties...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-018-2067-8
更新日期：2018-10-01 00:00:00
abstract：:The operation of bioreactors and the metabolism of microorganisms in biofilms or soil/sediment systems are strongly dictated by the transport processes therein. Nuclear magnetic resonance (NMR) spectroscopy or magnetic resonance imaging (MRI) allow nondestructive and noninvasive quantification and visualisation (in ca...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1038/sj/jim/7000087
更新日期：2001-01-01 00:00:00
abstract：:During the biotransformation of castor oil into gamma-decalactone, R. aurantiaca produced both the lactone form and its precursor (4-hydroxydecanoic acid). After six days of culture, a maximum yield of gamma-decalactone of 6.5 g/l was obtained. The parameters of gamma-decalactone adsorption on three Macronet resins (M...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-009-0659-z
更新日期：2010-02-01 00:00:00
abstract：:The bacteria causing foaming in activated sludge plants are considered to be hydrophobic, and their hydrophobicity is assumed to be a crucial factor in their foam-forming ability. This study showed no consistent relationship between cell surface hydrophobicity (CSH), as determined by microbial adherence to hydrocarbon...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1038/sj/jim/7000241
更新日期：2002-05-01 00:00:00
abstract：:To improve the thermostability of a mesophilic GH family 10 xylanase, AuXyn10A, from Aspergillus usamii E001, its modification was performed by in silico design. Based on the comparison of B-factor values, a mutant xylanase ATXyn10 was predicted by substituting a segment YP from Tyr(25) to Pro(34) of AuXyn10A with the...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-014-1463-y
更新日期：2014-08-01 00:00:00
abstract：:The conjugation protocols in myxobacterium Sorangium cellulosum are often inapplicable due to the strain-specific sensitivity to the presence of Escherichia coli cells or the resistances to many antibiotics. Here we report that the conjugative transfer of the mobilizable plasmid pCVD442 from E. coli DH5alpha (lambda p...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-008-0395-9
更新日期：2008-10-01 00:00:00
abstract：:Natural product discovery in the microbial world has historically been biased toward aerobes. Recent in silico analysis demonstrates that genomes of anaerobes encode unexpected biosynthetic potential for natural products, however, chemical data on natural products from the anaerobic world are extremely limited. Here, ...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s10295-018-2086-5
更新日期：2019-03-01 00:00:00
abstract：:Genistin and daidzein exhibit a protective effect on DNA damage and inhibit cell proliferation. Glycosylation and malonylation of the compounds increase water solubility and stability. Constructed pET15b-GmIF7GT and pET28a-GmIF7MAT were used for the transformation of Escherichia coli and bioconversion of genistein and...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-014-1504-6
更新日期：2014-11-01 00:00:00
abstract：:Using genetic engineering, the Vitreoscilla (bacterial) hemoglobin gene (vgb) was integrated stably into the chromosomes of Pseudomonas aeruginosa and Burkholderia sp. strain DNT. This was done for both wild type vgb and two site-directed mutants of vgb that produce Vitreoscilla hemoglobin (VHb) with lowered oxygen af...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-005-0215-4
更新日期：2005-04-01 00:00:00
abstract：:The production of arachidonic acid was studied in the fungus Mortierella alpina using an inexpensive medium. Glucose derived from maize starch hydrolysate was the sole carbon source and defatted soybean meal and sodium nitrate were the nitrogen sources. Optimal arachidonic acid yield (1.47 g l(-1)) was observed at a g...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-002-0013-1
更新日期：2003-01-01 00:00:00
abstract：:Keratinases are proteolytic enzymes capable of degrading insoluble keratins. The importance of these enzymes is being increasingly recognized in fields as diverse as animal feed production, textile processing, detergent formulation, leather manufacture, and medicine. To enhance the thermostability of Bacillus lichenif...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-013-1268-4
更新日期：2013-07-01 00:00:00
abstract：:The discharge of bromate, a suspected carcinogen, will be restricted in the near future. To assess the possibility of biotechnological treatment of bromate-containing wastewaters, the removal of bromate by chlorate-reducing microorganisms was studied. The removal of bromate and chlorate was studied in laboratory gas-l...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-004-0188-8
更新日期：2005-01-01 00:00:00
abstract：:A closed-circuit microbial fuel cell (C-MFC) was operated to investigate the electron flux under fed-batch mode, and the results were compared to those of open-circuit MFC (O-MFC) and a fermentation reactor (F-reactor). The current was the largest electron sink (52.7% of influent SCOD) in C-MFC, whereas biomass and me...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-015-1629-2
更新日期：2015-07-01 00:00:00
abstract：:The present study deals with the production of citric acid from a ram horn peptone (RHP) by Aspergillus niger NRRL 330. A medium from RHP and a control medium (CM) were compared for citric acid production using A. niger in a batch culture. For this purpose, first, RHP was produced. Ram horns were hydrolyzed by treatme...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-004-0147-4
更新日期：2004-07-01 00:00:00
abstract：:The effects of cell immobilization on the secretion of extracellular proteases and glucoamylase production by Aspergillus niger were investigated under a variety of immobilization techniques and culture conditions. Immobilization was achieved by means of cell attachment on metal surfaces or spore entrapment and subseq...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1038/sj.jim.7000289
更新日期：2002-11-01 00:00:00
abstract：:Microbes exchange electrons with their extracellular environment via direct or indirect means. This exchange is bidirectional and supports essential microbial oxidation-reduction processes, such as respiration and photosynthesis. The microbial capacity to use electrons from insoluble electron donors, such as redox-act...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s10295-020-02309-0
更新日期：2020-10-01 00:00:00
