Accumulation of organic acids in cultivations of Neisseria meningitidis C.


:Aiming at the industrial production of serogroup C meningococcal vaccine, different experimental protocols were tested to cultivate Neisseria meningitidis C and to investigate the related organic acid release. Correlations were established between specific rates of acetic acid and lactic acid accumulation and specific growth rate, during cultivations carried out on the Frantz medium in a 13 l bioreactor at 35 degrees C, 0.5 atm, 400 rpm and air flowrate of 2 l min(-1). A first set of nine batch runs was carried out: (1) with control of dissolved oxygen (O2) at 10% of its saturation point, (2) with control of pH at 6.5, and (3) without any control, respectively. Additional fed-batch or partial fed-batch cultivations were performed without dissolved O2 control, varying glucose concentration from 1.0 to 3.0 g l(-1), nine of which without pH control and other two with pH control at 6.5. No significant organic acid level was detected with dissolved O2 control, whereas acetic acid formation appeared to depend on biomass growth either in the absence of any pH and dissolved O2 control or when the pH was kept at 6.5. Under these last conditions, lactic acid was released as well, but it did not seem to be associated to biomass growth. A survey of possible metabolic causes of this behavior suggested that N. meningitidis may employ different metabolic pathways for the carbon source uptake depending on the cultivation conditions.


Baruque-Ramos J,Hiss H,Converti A,Gonçalves VM,Raw I




Has Abstract


2006-10-01 00:00:00












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    abstract::Production of recombinant protein bio-insecticides on a commercial scale can only be cost effective if host strains with very high expression levels are available. A recombinant fusion protein containing an arthropod toxin, ω-hexatoxin-Hv1a, (from funnel web spider Hadronyche versuta) linked to snowdrop lectin (Galant...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Pyati P,Fitches E,Gatehouse JA

    更新日期:2014-08-01 00:00:00

  • A comparison of stress tolerance in YPD and industrial lignocellulose-based medium among industrial and laboratory yeast strains.

    abstract::In general, it is believed that fermentation by yeast under harsh industrial conditions, especially if substrates such as wood hydrolysate or lignocellulosic substrates are used, requires the use of so-called industrial strains. In order to check whether this is always true, a comparison of performance was made using ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Albers E,Larsson C

    更新日期:2009-08-01 00:00:00

  • Insights into the fluoride-resistant regulation mechanism of Acidithiobacillus ferrooxidans ATCC 23270 based on whole genome microarrays.

    abstract::Acidophilic microorganisms involved in uranium bioleaching are usually suppressed by dissolved fluoride ions, eventually leading to reduced leaching efficiency. However, little is known about the regulation mechanisms of microbial resistance to fluoride. In this study, the resistance of Acidithiobacillus ferrooxidans ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Ma L,Li Q,Shen L,Feng X,Xiao Y,Tao J,Liang Y,Yin H,Liu X

    更新日期:2016-10-01 00:00:00

  • Poly(hydroxyalkanoate) synthase genotype and PHA production of Pseudomonas corrugata and P. mediterranea.

    abstract::A collection of Pseudomonas corrugata and P. mediterranea strains, two closely related species, was evaluated for the presence and variability of pha loci. Using PCR methods that specifically amplify segments of medium-chain-length poly(hydroxyalkanoate) (mcl-PHA) synthase genes, we demonstrated the presence of phaC1 ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Solaiman DK,Catara V,Greco S

    更新日期:2005-02-01 00:00:00

  • Five gene products are required for assembly of the central pyrrole moiety of coumermycin A1.

    abstract::Coumermycin A1 is an aminocoumarin antibiotic produced by Streptomyces rishiriensis. It exhibits potent antibacterial and anticancer activity. The coumermycin A1 molecule contains two terminal 5-methyl-pyrrole-2-carboxylic acid moieties and one central 3-methylpyrrole-2,4-dicarboxylic acid moiety (CPM). While the bios...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Novotna J,Gust B,Kulik A,Spizek J,Heide L

    更新日期:2013-08-01 00:00:00

  • Construction of advanced producers of first- and second-generation ethanol in Saccharomyces cerevisiae and selected species of non-conventional yeasts (Scheffersomyces stipitis, Ogataea polymorpha).

    abstract::This review summarizes progress in the construction of efficient yeast ethanol producers from glucose/sucrose and lignocellulose. Saccharomyces cerevisiae is the major industrial producer of first-generation ethanol. The different approaches to increase ethanol yield and productivity from glucose in S. cerevisiae are ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Ruchala J,Kurylenko OO,Dmytruk KV,Sibirny AA

    更新日期:2020-01-01 00:00:00

  • Optimization of process parameters for the production of carbonyl reductase by Candida viswanathii in a laboratory-scale fermentor.

    abstract::The effect of pH, aeration and mixing on the growth and production of carbonyl reductase by Candida viswanathii was investigated in a 6.6-l fermentor. Controlling the pH at 8.0 had a very significant effect on the enzyme production. Aeration and agitation influenced the dissolved oxygen concentration which in turn aff...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Soni P,Kansal H,Banerjee UC

    更新日期:2008-03-01 00:00:00

  • Bifunctional xylanases and their potential use in biotechnology.

    abstract::Plant cell walls are comprised of cellulose, hemicellulose and other polymers that are intertwined. This complex structure acts as a barrier to degradation by single enzyme. Thus, a cocktail consisting of bi and multifunctional xylanases and xylan debranching enzymes is most desired combination for the efficient utili...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Khandeparker R,Numan MT

    更新日期:2008-07-01 00:00:00

  • Heterologous production of a new lasso peptide brevunsin in Sphingomonas subterranea.

    abstract::A shuttle vector pHSG396Sp was constructed to perform gene expression using Sphingomonas subterranea as a host. A new lasso peptide biosynthetic gene cluster, derived from Brevundimonas diminuta, was amplified by PCR and integrated to afford a expression vector pHSG396Sp-12697L. The new lasso peptide brevunsin was suc...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Kodani S,Hemmi H,Miyake Y,Kaweewan I,Nakagawa H

    更新日期:2018-11-01 00:00:00

  • Molecular and phenotypic comparison of phaeochromycin-producing strains of Streptomyces phaeochromogenes and Streptomyces ederensis.

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Ritacco FV,Eveleigh DE

    更新日期:2008-09-01 00:00:00

  • Enhancement of Streptomyces transglutaminase activity and pro-peptide cleavage efficiency by introducing linker peptide in the C-terminus of the pro-peptide.

    abstract::Streptomyces transglutaminase (TGase) has been widely used in food, pharmaceutical and textile industries. Streptomyces TGase is naturally synthesized as zymogen (pro-TGase), which is then processed to produce active enzyme by removing its N-terminal pro-peptide. Although the pro-peptide is essential for TGase folding...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Chen K,Liu S,Wang G,Zhang D,Du G,Chen J,Shi Z

    更新日期:2013-04-01 00:00:00

  • Carbon-limited fed-batch production of medium-chain-length polyhydroxyalkanoates by a phaZ-knockout strain of Pseudomonas putida KT2440.

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Vo MT,Ko K,Ramsay B

    更新日期:2015-04-01 00:00:00

  • Genetic switches and related tools for controlling gene expression and electrical outputs of Geobacter sulfurreducens.

    abstract::Physiological studies and biotechnology applications of Geobacter species have been limited by a lack of genetic tools. Therefore, potential additional molecular strategies for controlling metabolism were explored. When the gene for citrate synthase, or acetyl-CoA transferase, was placed under the control of a LacI/IP...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Ueki T,Nevin KP,Woodard TL,Lovley DR

    更新日期:2016-11-01 00:00:00

  • Analysis of respiratory activity and carbon usage of a mutant of Azotobacter vinelandii impaired in poly-β-hydroxybutyrate synthesis.

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Jiménez L,Castillo T,Flores C,Segura D,Galindo E,Peña C

    更新日期:2016-08-01 00:00:00

  • Rapid and stable production of 2,3-butanediol by an engineered Saccharomyces cerevisiae strain in a continuous airlift bioreactor.

    abstract::Utilization of renewable feedstocks for the production of bio-based bulk chemicals, such as 2,3-butanediol (2,3-BDO), by engineered strains of the non-pathogenic yeast, Saccharomyces cerevisiae, has recently become an attractive option. In this study, to realize rapid production of 2,3-BDO, a flocculent, 2,3-BDO-produ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Yamada R,Nishikawa R,Wakita K,Ogino H

    更新日期:2018-05-01 00:00:00

  • DNA assembly techniques for next-generation combinatorial biosynthesis of natural products.

    abstract::Natural product scaffolds remain important leads for pharmaceutical development. However, transforming a natural product into a drug entity often requires derivatization to enhance the compound's therapeutic properties. A powerful method by which to perform this derivatization is combinatorial biosynthesis, the manipu...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Cobb RE,Ning JC,Zhao H

    更新日期:2014-02-01 00:00:00

  • Yeast culture collections of the world: meeting the needs of industrial researchers.

    abstract::The importance of selecting optimal yeast strains for research or industrial applications is often underestimated. For example, utilizing a strain background that already provides the desired stress tolerance or nutrient utilization profile can eliminate costly strain optimization. Yeast culture collections can provid...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章,评审


    authors: Boundy-Mills K

    更新日期:2012-05-01 00:00:00

  • Antimicrobial susceptibility of Lactobacillus species isolated from commercial ethanol plants.

    abstract::Bacterial contamination of commercial fermentation cultures is a common and costly problem to the fuel ethanol industry. Antimicrobials such as virginiamycin (VIR) and penicillin (PEN) are frequently used to control contamination but there are little data available on the susceptibility of bacterial contaminants to th...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Bischoff KM,Skinner-Nemec KA,Leathers TD

    更新日期:2007-11-01 00:00:00

  • Growth and bacteriocin production by Enterococcus faecium DPC1146 in batch and continuous culture.

    abstract::Production of the bacteriocin enterocin 1146 (E1146) by Enterococcus faecium DPC1146 was studied in batch and continuous fermentation. Growth was strongly inhibited by lactic acid. In batch fermentations maximum E1146 activity (2.8 MBU L-1) was obtained in 9 h with 20 g L-1 glucose. Increase in initial glucose concent...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Parente E,Brienza C,Ricciardi A,Addario G

    更新日期:1997-01-01 00:00:00

  • Assessment of physiological state of microorganisms in activated sludge with flow cytometry: application for monitoring sludge production minimization.

    abstract::Many sludge reduction processes have been studied for the minimization of sludge production in biological wastewater treatment. The investigations on most of these processes have monitored the increase of the soluble chemical oxygen demand, the sludge mass reduction, or the decrease of the floc size, but little inform...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Prorot A,Eskicioglu C,Droste R,Dagot C,Leprat P

    更新日期:2008-11-01 00:00:00

  • On-line biomass measurements in bioreactor cultivations: comparison study of two on-line probes.

    abstract::Two on-line probes for biomass measurement in bioreactor cultivations were evaluated. One probe is based on near infrared (NIR) light absorption and the other on dielectric spectroscopy. The probes were used to monitor biomass production in cultivations of several different microorganisms. Differences in NIR probe res...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Kiviharju K,Salonen K,Moilanen U,Meskanen E,Leisola M,Eerikäinen T

    更新日期:2007-08-01 00:00:00

  • Cell surface hydrophobicity and mycolic acid composition of Rhodococcus strains isolated from activated sludge foam.

    abstract::The bacteria causing foaming in activated sludge plants are considered to be hydrophobic, and their hydrophobicity is assumed to be a crucial factor in their foam-forming ability. This study showed no consistent relationship between cell surface hydrophobicity (CSH), as determined by microbial adherence to hydrocarbon...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Stratton HM,Brooks PR,Griffiths PC,Seviour RJ

    更新日期:2002-05-01 00:00:00

  • The performance of fungal xylan-degrading enzyme preparations in elemental chlorine-free bleaching for Eucalyptus pulp.

    abstract::Cellulase-free xylan-degrading enzyme preparations from Acrophialophora nainiana, Humicola grisea var. thermoidea and two Trichoderma harzianum strains were used as bleaching agents for Eucalyptus kraft pulp, prior to a chlorine dioxide and alkaline bleaching sequence. In comparison to the control sequence (performed ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Medeiros RG,Silva FG Jr,Salles BC,Estelles RS,Filho EX

    更新日期:2002-04-01 00:00:00

  • Functional expression of a single-chain antibody fragment against human epidermal growth factor receptor 2 (HER2) in Escherichia coli.

    abstract::The human epidermal growth factor receptor (HER) family plays an important role in cell growth and signaling and alteration of its function has been demonstrated in many different kinds of cancer. Receptor dimerization is necessary for the HER signal transduction pathway and tyrosine kinase activity. Recently, several...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Akbari V,Mir Mohammad Sadeghi H,Jafrian-Dehkordi A,Abedi D,Chou CP

    更新日期:2014-06-01 00:00:00

  • Rapidly directional biotransformation of tauroursodeoxycholic acid through engineered Escherichia coli.

    abstract::Bear bile powder is a precious medicinal material. It is characterized by high content of tauroursodeoxycholic acid (TUDCA) at a ratio of 1.0-1.5 to taurochenodeoxycholic acid (TCDCA). Here, we reported the biotransformation of tauroursodeoxycholic acid (TUDCA) through Escherichia coli engineered with a two-step mimic...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Shi J,Wang J,Yu L,Yang L,Zhao S,Wang Z

    更新日期:2017-07-01 00:00:00

  • Identification of metabolites produced from N-phenylpiperazine by Mycobacterium spp.

    abstract::Mycobacterium sp. 7E1B1W and seven other mycobacterial strains known to degrade hydrocarbons were investigated to determine their ability to metabolize the piperazine ring, a substructure found in many drugs. Cultures were grown at 30 degrees C in tryptic soy broth and dosed with 3.1 mM N-phenylpiperazine hydrochlorid...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Adjei MD,Deck J,Heinze TM,Freeman JP,Williams AJ,Sutherland JB

    更新日期:2007-03-01 00:00:00

  • Heterotrophic nitrogen removal by Providencia rettgeri strain YL.

    abstract::Providencia rettgeri strain YL was found to be efficient in heterotrophic nitrogen removal under aerobic conditions. Maximum removal of NH(4) (+)-N occurred under the conditions of pH 7 and supplemented with glucose as the carbon source. Inorganic ions such as Mg(2+), Mn(2+), and Zn(2+) largely influenced the growth a...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Zhao B,He YL,Huang J,Taylor S,Hughes J

    更新日期:2010-06-01 00:00:00

  • Construction of the astaxanthin biosynthetic pathway in a methanotrophic bacterium Methylomonas sp. strain 16a.

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Ye RW,Yao H,Stead K,Wang T,Tao L,Cheng Q,Sharpe PL,Suh W,Nagel E,Arcilla D,Dragotta D,Miller ES

    更新日期:2007-04-01 00:00:00

  • A very efficient bioconversion of soybean phytosterols mixtures to androstanes by mycobacteria.

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    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Pérez C,Falero A,Duc HL,Balcinde Y,Hung BR

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  • Carboxyl-terminal domain characterization of polyene-specific P450 hydroxylase in Pseudonocardia autotrophica.

    abstract::A polyene compound NPP identified in Pseudonocardia autotrophica was shown to contain an aglycone identical to nystatin, but to harbor a unique disaccharide moiety that led to higher solubility and reduced hemolytic activity. Recently, it was revealed that the final step of NPP (nystatin-like polyene) biosynthesis is ...

    journal_title:Journal of industrial microbiology & biotechnology

    pub_type: 杂志文章


    authors: Kim MK,Won HJ,Kim HJ,Choi SS,Lee HS,Kim P,Kim ES

    更新日期:2016-11-01 00:00:00