当前位置： SCI文献检索 > JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY期刊下所有文献 > Construction of a "nutrition supply-detoxification" coculture consortium for medium-chain-length polyhydroxyalkanoate production with a glucose-xylose mixture.

Construction of a "nutrition supply-detoxification" coculture consortium for medium-chain-length polyhydroxyalkanoate production with a glucose-xylose mixture.

Abstract：

:In this study, we constructed a coculture consortium comprising engineered Pseudomonas putida KT2440 and Escherichia coli MG1655. Provision of "related" carbon sources and synthesis of medium-chain-length polyhydroxyalkanoates (mcl-PHAs) were separately assigned to these strains via a modular construction strategy. To avoid growth competition, a preference for the use of a carbon source was constructed. Further, the main intermediate metabolite acetate played an important role in constructing the expected "nutrition supply-detoxification" relationship between these strains. The coculture consortium showed a remarkable increase in the mcl-PHA titer (0.541 g/L) with a glucose-xylose mixture (1:1). Subsequently, the titer of mcl-PHA produced by the coculture consortium when tested with actual lignocellulosic hydrolysate (0.434 g/L) was similar to that achieved with laboratory sugars' mixture (0.469 g/L). These results indicate a competitive potential of the engineered E. coli-P. putida coculture consortium for mcl-PHA production with lignocellulosic hydrolysate.

journal_name

J Ind Microbiol Biotechnol

journal_title

Journal of industrial microbiology & biotechnology

authors

Liu Y,Yang S,Jia X

doi

10.1007/s10295-020-02267-7

subject

Has Abstract

pub_date

2020-03-01 00:00:00

pages

343-354

issue

3

eissn

1367-5435

issn

1476-5535

pii

10.1007/s10295-020-02267-7

journal_volume

47

pub_type

杂志文章

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