Abstract:
:The World Health Organization's Expanded Programme on Immunization has led to a dramatic rise in worldwide vaccination rates over the past 40years, yet 19.4 million infants remain underimmunized each year. Many of these infants have received at least one vaccine dose but may remain unprotected because they did not receive subsequent booster doses due to logistical challenges. This study aimed to develop injectable controlled release microparticles with kinetics that mimic common vaccine dosing regimens consisting of large antigen doses administered periodically over the course of months in order to eliminate the need for boosters. Sixteen poly(lactic-co-glycolic acid) (PLGA) microsphere formulations containing bovine serum albumin (BSA) as a model vaccine antigen were screened in vitro to determine their respective release kinetics. Three formulations that exhibited desirable pulsatile release profiles were then selected for studying immunogenicity in mice. Two low-dose microsphere formulations induced peak anti-BSA IgG antibody titers of 13.9±1.3 and 13.7±2.2 log2 compared to 15.5±1.5 log2 for a series of three bolus injections delivered at 0, 4, and 8weeks with an equivalent cumulative dose. Similarly, high-dose formulations induced peak antibody titers that were 16.1±2.1 log2 compared to 17.7±2.2 log2 for controls. All three microparticle formulations studied in vivo induced peak antibody titers that were statistically similar to bolus controls. These results suggest that pulsatile antigen release from polymeric microparticles is a promising approach for single-injection vaccination, which could potentially reduce the logistical burden associated with immunization in the developing world.
journal_name
Vaccinejournal_title
Vaccineauthors
Guarecuco R,Lu J,McHugh KJ,Norman JJ,Thapa LS,Lydon E,Langer R,Jaklenec Adoi
10.1016/j.vaccine.2017.05.094subject
Has Abstractpub_date
2018-05-24 00:00:00pages
3161-3168issue
22eissn
0264-410Xissn
1873-2518pii
S0264-410X(17)30764-8journal_volume
36pub_type
杂志文章相关文献
VACCINE文献大全abstract::To compare the ability of a native and a recombinant preparation of the major outer membrane protein of Chlamydia trachomatis mouse pneumonitis (MoPn; Ct-nMOMP and Ct-rMOMP) to protect against an intranasal (i.n.) challenge, BALB/c mice were vaccinated by the intramuscular (i.m.) and subcutaneous (s.c.) routes using C...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.05.008
更新日期:2009-08-06 00:00:00
abstract::Measles transmission has been well documented in healthcare facilities. Healthcare personnel who are unvaccinated and who lack other evidence of measles immunity put themselves and their patients at risk for measles. We conducted a systematic literature review of measles vaccination policies and their implementation i...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2013.11.005
更新日期:2014-08-27 00:00:00
abstract::The vaccination program in Zambia includes one dose of measles vaccine at 9 months of age. The objective of this study was to compare the cost-effectiveness of the current one-dose measles vaccination program with an immunization schedule in which a second dose is provided either through routine health services or thr...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2003.07.007
更新日期:2004-01-02 00:00:00
abstract::Previous studies with the malaria vaccine RTS,S/AS02(A) in young children in a malaria endemic area of Mozambique have shown it to have a promising safety profile and to reduce the risk of Plasmodium falciparum infection and disease. In this study, we assessed the antibody responses to the P. falciparum and hepatitis ...
journal_title:Vaccine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.vaccine.2011.03.041
更新日期:2011-08-11 00:00:00
abstract:BACKGROUND:A vaccine manufacturer in China and regulatory authorities have been the focus of widespread outrage due to a vaccine scandal. We conducted a rapid survey during a time of intense mainstream and social media attention to determine whether the public's confidence in vaccines was affected. METHODS:We selected...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2019.07.052
更新日期:2019-08-23 00:00:00
abstract::ADVAX is a DNA-based candidate HIV vaccine that was safe but weakly immunogenic when delivered intramuscularly (IM) in humans. Studies were performed in animal models to determine whether an alternative delivery method, in vivo electroporation (EP), could improve the immunogenicity of ADVAX while maintaining an accept...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.11.011
更新日期:2011-01-17 00:00:00
abstract::Gram-negative bacterial outer membrane proteins (Omps) have an important role in pathogenesis and signal reception. We previously reported that Acinetobacter OmpA (AbOmpA) induced maturation of bone marrow-derived dendritic cells (BMDCs) and that AbOmpA-primed DCs produced IL-12 which generated Th1 CD4(+) T-cells. We ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.01.036
更新日期:2011-03-16 00:00:00
abstract::Protein based therapeutics have enjoyed great success over the past decade. Unfortunately, with this clinical success comes a heavy price tag, owing to the inherently high costs of capitalization and production using mammalian cell fermentation. To address this problem, we have begun developing a system for the expres...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2004.11.013
更新日期:2005-03-07 00:00:00
abstract::Oral, replication-competent Ad-HIV vaccines are advancing to human trials. Previous evaluation of protective efficacy in non-human primates has primarily followed upper respiratory tract administrations. Here we compared sequential oral (O/O) versus intranasal/oral (I/O) priming of rhesus macaques with Ad5 host range ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2007.09.017
更新日期:2007-11-19 00:00:00
abstract::Recombinant poxviruses are well suited for the development of new vaccine vectors. Our previous data supported the idea that Myxomavirus (MYXV) is efficient at priming antibody responses in sheep. To provide definitive evidence on the potential of MYXV for vaccination against infectious diseases in ruminants, we inves...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.12.108
更新日期:2012-02-21 00:00:00
abstract::One hundred and eight primary school students who had been vaccinated with three doses of a plasma-derived hepatitis B vaccine were randomly divided into two groups, to receive a booster dose of either the same vaccine or of a recombinant yeast-derived vaccine. The pre-booster anti-hepatitis B surface antigen (HBs) ge...
journal_title:Vaccine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0264-410x(91)90294-g
更新日期:1991-10-01 00:00:00
abstract::Aluminum salts such as aluminum oxyhydroxide and aluminum hydroxyphosphate are commonly used human vaccine adjuvants. In an effort to improve the adjuvant activity of aluminum salts, we previously showed that the adjuvant activity of aluminum oxyhydroxide nanoparticles is significantly more potent than that of aluminu...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.04.081
更新日期:2016-06-08 00:00:00
abstract:INTRODUCTION:Endemic measles persists in China, despite >95% reported coverage of two measles-containing vaccine doses and nationwide campaign that vaccinated more than 100 million children in 2010. In 2011, almost half of the 9943 measles cases in China occurred in children eligible for measles vaccination. We conduct...
journal_title:Vaccine
pub_type: 杂志文章,多中心研究
doi:10.1016/j.vaccine.2016.02.005
更新日期:2016-12-12 00:00:00
abstract::A large, randomized, placebo-controlled clinical trial in Italy on two three-component pertussis vaccines, given as DTaP in infancy, one manufactured by SmithKline and Beecham (SB) and one by Chiron Biocine (CB), found each vaccine to be 84% efficacious through the average age of 24 months. The cohort of children enro...
journal_title:Vaccine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0264-410x(98)00040-1
更新日期:1998-08-01 00:00:00
abstract::Rift Valley fever virus (RVFV), a member of the family Bunyaviridae, causes severe to fatal disease in newborn ruminants, as well as abortions in pregnant animals; both preventable by vaccination. Availability of a challenge model is a pre-requisite for vaccine efficacy trials. Several modes of inoculation with RVFV Z...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.02.066
更新日期:2014-04-25 00:00:00
abstract::Since HLA-restricted cytotoxic T-cell responses select specific polymorphisms in HIV-1 sequences and HLA diversity is relatively static in human populations, we investigated the use of peptide epitopes based on sites of HLA-associated adaptation in HIV-1 sequences to stimulate and detect T-cell responses ex vivo. Thes...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.06.091
更新日期:2010-08-23 00:00:00
abstract:AIM:Design and immunogenicity assessment of the combined vaccine candidate against zoonotic hepatitis E virus (HEV) and foot-and-mouth disease virus (FMDV). METHODS:Using the molecular cloning technology, we produced and purified 9 HEV ORF2-truncated proteins (HEV genotype 4). Then, we compared their thermal stability...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2019.09.036
更新日期:2019-10-31 00:00:00
abstract::Infectious laryngotracheitis virus (ILTV) is an alphaherpesvirus that causes acute respiratory disease in chickens worldwide. The virus is horizontally transmitted and causes large outbreaks of disease. Recent studies have shown that a glycoprotein G deficient candidate vaccine strain of ILTV (ΔgG ILTV) is safe and pr...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.06.002
更新日期:2011-08-05 00:00:00
abstract::The hemagglutinin/protease (HA/P) seems to be an attractive locus for the insertion of heterologous tags in live cholera vaccine strains. A deltaCTXphi spontaneous mutant derived from a pathogenic strain of O139 Vibrio cholerae was sequentially manipulated to obtain hapA Colon, two colons celA derivatives which were l...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(02)00412-7
更新日期:2003-03-07 00:00:00
abstract:BACKGROUND:Vaccinations have been suggested to be associated with increased risk of allergic diseases. Tetanus vaccination is one of the most frequently administered vaccines as a part of wound management and was also found to be associated with increased serum IgE levels. We hypothesized that the vaccination modifies ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.10.068
更新日期:2016-12-12 00:00:00
abstract::NetB (necrotic enteritis toxin B) is a recently identified β-pore-forming toxin produced by Clostridium perfringens. This toxin has been shown to play a major role in avian necrotic enteritis. In recent years, a dramatic increase in necrotic enteritis has been observed, especially in countries where the use of antimic...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2013.05.063
更新日期:2013-08-20 00:00:00
abstract:BACKGROUND:Children are scheduled to receive 18-20 immunizations before their 18th birthday in England and Wales; this approximates to 13 million vaccines administered per annum. Each immunization represents a potential opportunity for immunization-related error and effective immunization is imperative to maintain the ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.06.068
更新日期:2015-07-31 00:00:00
abstract::The prevalence of seropositive individuals that makes costs of vaccinating all individuals equal to that for screening and vaccination of susceptible individuals is defined as the critical prevalence of antibodies (p*). Screening and vaccination is more efficient when the prevalence of seropositive individuals (p) in ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2004.03.048
更新日期:2004-09-28 00:00:00
abstract::DNA vectors can be used to deliver vaccine antigens that stimulate effective protective immunity in mice, but in larger, outbred animal species, the protective efficacy is lower or large doses of DNA are required. These data demonstrate that porcine interleukin-3 (IL-3) when delivered to pigs by DNA vector or in low d...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2006.01.023
更新日期:2006-04-12 00:00:00
abstract::Six adjuvant formulations were compared for their ability to potentiate the primary and memory antibody responses in mice to three companion animal vaccine immunogens--feline leukemia virus (FeLV), feline immunodeficiency virus (FIV), and a recombinantly-derived heartworm antigen. The combination of a novel bacterial ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(97)00136-9
更新日期:1997-12-01 00:00:00
abstract::It is generally accepted that the introduction of hepatitis B vaccine to national programmes of immunization in developing countries is desirable, but it has so far been constrained by the high cost of the vaccine. To determine the potential minimal costs of the vaccine, a study has been carried out of the production ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(90)90101-q
更新日期:1990-08-01 00:00:00
abstract:BACKGROUND:Typhoid fever remains a major public health problem in low- and middle-income countries where children aged 2-14 years bear the greatest burden. Vi polysaccharide is poorly immunogenic in children <2 years of age, and protection in adults is modest. The limitations of Vi polysaccharide vaccines can be overco...
journal_title:Vaccine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.vaccine.2018.05.038
更新日期:2018-06-18 00:00:00
abstract::The effect of passively transferred RSV immune serum on the antibody response to a single dose of purified RSV fusion (F) and large (G) glycoproteins was studied in cotton rats. Passively transferred antibody that achieved serum antibody levels similar to those seen in newborn human infants resulted in a seven- to eig...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(91)90151-u
更新日期:1991-03-01 00:00:00
abstract::Persistence of antibodies 4 years after vaccination with measles, mumps and rubella (MMR) vaccine from three different manufacturers was compared in 475 children who received a single injection of vaccine when aged 12-18 months. Antibodies to measles and mumps were measured using a plaque reduction neutralisation assa...
journal_title:Vaccine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0264-410x(94)00086-3
更新日期:1995-06-01 00:00:00
abstract::Hepatitis B is a parenterally and sexually transmitted disease of global importance. It associates with hepatitis D in a consistent proportion of cases. The disease most frequently runs a subclinical and anicteric course, with a significant number of cases that become chronic. Chronic hepatitis may progress to cirrhos...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/0264-410x(93)90219-n
更新日期:1993-01-01 00:00:00