Abstract：
:The success of mesenchymal stem cell transplantation for proctitis depends not only on cell donors but also on host microenvironmental factors, which play a major role in conditioning mesenchymal stem cell immunosuppressive action and repair. This study sought to determine if flagellin, a TLR5 ligand, can enhance the mesenchymal stem cell treatment efficacy in radiation-induced proctitis. With the use of a colorectal model of 27 Gy irradiation in rats, we investigated and compared the effects on immune capacity and remodeling at 28 d after irradiation of the following: 1) systemic mesenchymal stem cell (5 × 10(6)) administration at d 7 after irradiation, 2) administration of flagellin at d 3 and systemic mesenchymal stem cell administration at d 7, and 3) in vitro preconditioning of mesenchymal stem cells with flagellin, 24 h before their administration on d 7. The mucosal CD8(+) T cell population was normalized after treatment with flagellin-preconditioned mesenchymal stem cells or flagellin plus mesenchymal stem cells, whereas mesenchymal stem cells alone did not alter the radiation-induced elevation of CD8(+) T cell frequency. Mesenchymal stem cell treatment returned the irradiation-elevated frequency of CD25(+) cells in the mucosa-to-control levels, whereas both flagellin-preconditioned mesenchymal stem cell and flagellin-plus-mesenchymal stem cell treatment each significantly increased not only CD25(+) cell frequency but also forkhead box p3 and IL-2Rα expression. Specifically, IL-10 was overexpressed after flagellin-preconditioned mesenchymal stem cell treatment. Analysis of collagen expression showed that the collagen type 1/collagen type 3 ratio, an indicator of wound-healing maturation, was low in the irradiated and mesenchymal stem cell-treated groups and returned to the normal level only after the flagellin-preconditioned mesenchymal stem cell treatment. This was associated with a reduction in myofibroblast accumulation. In a proctitis model, flagellin-preconditioned mesenchymal stem cells improved colonic immune capacity and enhanced tissue remodeling.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Linard C,Strup-Perrot C,Lacave-Lapalun JV,Benderitter Mdoi
10.1189/jlb.3A0915-393Rsubject
Has Abstractpub_date
2016-09-01 00:00:00pages
569-80issue
3eissn
0741-5400issn
1938-3673pii
jlb.3A0915-393Rjournal_volume
100pub_type
杂志文章abstract：:Although class A type I/II scavenger receptor (SR-A) is involved in numerous macrophage functions, its signaling ability remains uncertain. We used monoclonal antibodies (mAb) to specifically stimulate receptors on mouse alveolar (AMs) and peritoneal macrophages (PMs). Immobilized anti-SR-A (2F8) and anti-FcgammaR II/...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.0504270
更新日期：2004-11-01 00:00:00
abstract：:The absence or reduction of CFTR function causes CF and results in a pulmonary milieu characterized by bacterial colonization and unresolved inflammation. The ineffectiveness at controlling infection by species such as Pseudomonas aeruginosa suggests defects in innate immunity. Macrophages, neutrophils, and DCs have a...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.0412188
更新日期：2012-11-01 00:00:00
abstract：:HL-60 cells are promyelocytic leukemia cells that respond to culture conditions with differentiation into granulocytelike or macrophagelike phagocytes. O2 metabolism is critical to the microbicidal function of phagocytic cells. O2 metabolism was studied in HL-60 cells differentiated with dimethylsulfoxide (Me2SO) and ...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.43.2.140
更新日期：1988-02-01 00:00:00
abstract：:Transition metals, including zinc, are essential to all living organisms. They are also toxic in high amounts, and their intracellular concentration must be tightly regulated. In this edition of JLB, Stocks et al. report that the zinc transporter, ZnT1 (SLC30A1) is induced by TLR4 activation in Mϕs, in which it contri...
journal_title：Journal of leukocyte biology
pub_type： 社论
doi：10.1002/JLB.4CE0520-256R
更新日期：2021-02-01 00:00:00
abstract：:We examined the relationship of I-A expression by normal murine macrophages to their immunoregulatory role on natural killer cell activity. Macrophages were isolated on the basis of plastic adherence; characterized on the basis of conventional markers such as phagocytic ability, cytoplasmic non-specific esterase activ...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.48.5.382
更新日期：1990-11-01 00:00:00
abstract：:Glucosamine, an amino monosaccharide naturally occurring in the connective and cartilage tissues, contributes to maintaining the strength, flexibility, and elasticity of these tissues. In recent years, glucosamine has been used widely to treat osteoarthritis in humans and animal models. Neutrophils, which usually func...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：
更新日期：2002-04-01 00:00:00
abstract：:It has been reported recently that granulocyte-colony stimulating factor (G-CSF) is degraded upon exposure to human neutrophil elastase (HNE), and this has a negative effect on the ability of the cytokine to promote the in vitro proliferation and maturation of CD34+ cells. This has important implications on the possib...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.0803378
更新日期：2004-03-01 00:00:00
abstract：:T helper cell type 1 (Th1)-polarizing cytokines are induced by Legionella pneumophila infection and are suppressed by pretreatment with marijuana cannabinoids (CB). Glucocorticoids and prostaglandin E2(PGE2) are also reported to suppress Th1 polarization and are induced by Delta9-tetrahydrocannabinol (THC), so their r...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.0304184
更新日期：2004-10-01 00:00:00
abstract：:PI(3)Kγ is thought to mediate leukocyte migration to injured tissues and may be important in the pathogenesis of various T-lymphocyte-dependent pathologies, including autoimmune and inflammatory diseases. The present study evaluated the relevance of PI(3)Kγ in donor cells for the pathogenesis of acute GVHD using a mod...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.0810464
更新日期：2011-06-01 00:00:00
abstract：:Neovascularization, the process of new blood vessel growth, is an important feature of many pathologic and physiologic processes. Monocytes were isolated from citrated blood buffy coat of healthy adult human donors on Ficoll-Hypaque gradients. Mononuclear cells from these gradients were fractionated on discontinuous P...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.39.2.233
更新日期：1986-02-01 00:00:00
abstract：:TNF is implicated in the suppression of neutrophil apoptosis during sepsis. Multiple signaling pathways are involved in TNF-mediated antiapoptotic signaling; a role for the MAP kinases (MAPK), ERK1/2, and p38 MAPK has been suggested. Antiapoptotic signaling is mediated principally through TNF receptor-1 (TNFR-1), and ...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.0406284
更新日期：2006-12-01 00:00:00
abstract：:A cell resistant to concentrations of mitomycin-C associated with the inactivation of many in vitro immune responses is described. This cell is responsive to concanavalin A (Con A), poorly adherent to nylon wool, present in relatively greater numbers in lymph node than other lymphoid tissues, and is relatively radiose...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.41.1.33
更新日期：1987-01-01 00:00:00
abstract：:Colorectal cancer is the second most common cause of cancer-related death. Novel prognostic factors should be identified and validated to refine the present tumor-node-metastasis system. The presence of immune cells infiltrating colorectal cancers is a common phenomenon. However, the current belief is that clinically ...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.1107773
更新日期：2008-10-01 00:00:00
abstract：:When mouse peritoneal cells in early phase-inflammatory exudates were cultured in vitro, the number of polymorphonuclear leukocytes (PMNs) rapidly decreased, whereas the number of macrophages gradually increased during culture. Macrophage growth was also induced by addition of effete PMNs or their debris to macrophage...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.39.5.489
更新日期：1986-05-01 00:00:00
abstract：:The Charcot-Leyden crystal (CLC) protein, a prominent cell constituent unique to eosinophils and basophils, possesses lysophospholipase activity. This activity and the extracellular deposition and formation of CLC in tissues and body fluids in association with eosinophils suggest an extracellular function for this pro...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.51.4.386
更新日期：1992-04-01 00:00:00
abstract：:Evidence that gamma/delta T cells play a broad, immunoregulatory role has been accumulating steadily. We show here that myeloid cells are disregulated after peritoneal infection with Listeria monocytogenes in mice lacking gamma/delta T cells. Inflammatory populations of neutrophils and monocytes recruited to the site ...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.1103574
更新日期：2004-07-01 00:00:00
abstract：:Over 1.4 million Americans have been diagnosed with inflammatory bowel disease (IBD), and ulcerative colitis (UC) makes up approximately half of those diagnoses. As a disease, UC cycles between periods of remission and flare, which is characterized by intense abdominal pain, increased weight loss, intestinal inflammat...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/JLB.4MA1217-506R
更新日期：2018-09-01 00:00:00
abstract：:Tumor necrosis factor (TNF)-stimulated gene 14 (TSG-14, also termed PTX3) encodes a secreted glycoprotein whose carboxy-terminal half shares sequence similarity with the pentraxin family of acute phase proteins (C-reactive protein and serum amyloid P component). We compared TSG-14 mRNA expression in cultures of murine...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.67.3.387
更新日期：2000-03-01 00:00:00
abstract：:In vivo, dendritic cells (DC) reside in direct proximity to extracellular matrix (ECM) proteins. Because ECM proteins affect morphology and function of a number of cell types, this study investigated potential effects of ECM proteins on functional properties of DC. DC were generated from murine bone marrow cultures, s...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.60.4.465
更新日期：1996-10-01 00:00:00
abstract：:Postnatal stem cells regulate the homeostasis of the majority of our tissues. They continuously generate new progenitors and mature, functional cells to replace old cells, which cannot assume the tissue function anymore and are eliminated. Blood, skin, gut mucosa, muscle, cartilage, nerves, cornea, retina, liver, and ...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章,评审
doi：10.1189/jlb.0505272
更新日期：2005-10-01 00:00:00
abstract：:Eosinophils were isolated from peritoneal lavages of repeated horse serum-injected guinea pigs or rhesus monkeys and from peripheral blood of normal human donors. Eicosanoid metabolism and chemotaxis by these cells were studied by in vitro techniques. Upon calcium ionophore stimulation guinea pig eosinophils released ...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.50.2.140
更新日期：1991-08-01 00:00:00
abstract：:Interleukin-1beta is a secreted protein that accumulates in the cytosol as an inactive precursor (pIL-1beta) before processing and release of biologically active protein. To understand the impact of this property on IL-1beta production, we examined the intracellular stability of pIL-1beta in lipopolysaccharide (LPS)-s...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：
更新日期：2000-07-01 00:00:00
abstract：:In the absence of IL-10, colonic inflammation ensues, which is characterized by high levels of IL-17. Here, we demonstrate a direct correlation between ICOS expression and IL-17 production in cIELs. IL-10(-/-) mice had increased numbers of cIELs and greater colon weight. Although the CD69 early activation antigen was ...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.0409238
更新日期：2010-02-01 00:00:00
abstract：:Human alveolar macrophages (AMO) have been investigated for their ability to produce three monokines, tumor necrosis factor-alpha (TNF), macrophage colony stimulating factor (CSF-1), and interleukin 1-beta (IL-1). No TNF activity was found in supernatants of unstimulated AMO cultured for 20 h, although TNF mRNA was de...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：
更新日期：1989-04-01 00:00:00
abstract：:The expression of beta 2-integrins on gammadelta T cells in naïve mice or those with experimental autoimmune encephalomyelitis (EAE) remains poorly characterized. We compared beta 2-integrin expression and cytokine production between gammadelta and alphabeta T cells over the acute course of EAE. We observed that unlik...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.0407263
更新日期：2008-01-01 00:00:00
abstract：:Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population of immature myeloid cells that accumulate during pathologic conditions, such as cancer. Patients diagnosed with advanced metastatic cancers have an average survival of 12-24 mo, a survival time that hasn't changed significantly in the past 30 yr. ...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章,评审
doi：10.1189/jlb.5VMR1116-458RRR
更新日期：2017-09-01 00:00:00
abstract：:The neutrophil inflammatory response can be altered profoundly by contact with extracellular matrix proteins (ECMs). We characterized functional responses (intracellular calcium, actin polymerization, degranulation, adhesion, and oxidative burst) of bovine neutrophils adhered to selected ECM proteins [collagen IV, lam...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：
更新日期：2002-05-01 00:00:00
abstract：:Mice with a naturally occurring Csfm(op)/Csfm(op) (op/op) gene mutation lack functional macrophage-colony stimulating factor (M-CSF) and are deficient of M-CSF-derived macrophages. They are severely monocytopenic, and their remaining M-CSF-independent macrophages were shown to differ in differentiation and distinct fu...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.12011003
更新日期：2003-05-01 00:00:00
abstract：:A combination of diclofenac and glucan administered repeatedly in a protective regimen in the course of repeated gamma irradiation of mice (6 x 2 Gy during 3 weeks) enhanced granulopoiesis and other indices of hematopoietic recovery investigated from 3 to 7 days after the last radiation exposure. Repeated administrati...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.53.2.185
更新日期：1993-02-01 00:00:00
abstract：:Mucosa represents the main site of pathogen/cell interactions. The two main types of cells forming the epithelial structure [epithelial cells and Langerhans cells (LC)] coordinate the first defense responses to avoid infection. To evaluate the involvement of epithelial cells in the early steps leading to a specific ad...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.0305147
更新日期：2005-07-01 00:00:00
