Characterization of CCL20 secretion by human epithelial vaginal cells: involvement in Langerhans cell precursor attraction.

Abstract:

:Mucosa represents the main site of pathogen/cell interactions. The two main types of cells forming the epithelial structure [epithelial cells and Langerhans cells (LC)] coordinate the first defense responses to avoid infection. To evaluate the involvement of epithelial cells in the early steps leading to a specific adaptive immune response, we have studied the interactions between vaginal epithelial and LC through the establishment of a human vaginal epithelial mucosa. We demonstrate that normal human vaginal epithelial cells constitutively secrete the chemokine macrophage inflammatory protein 3alpha/CC chemokine ligand 20 (CCL20), known to recruit LC precursors (LCps) selectively via its cognate CC chemokine receptor 6 (CCR6). This secretion is up-regulated by the proinflammatory cytokine interleukin-1beta through the nuclear factor-kappaB pathway. Similar results were obtained with the human vaginal epithelial cell line SiHa, which displays numerous homologies with normal vaginal cells. The chemotactic activity of the secreted CCL20 was demonstrated by its ability to attract LCp CCR6+. Moreover, the use of neutralizing polyclonal antibodies directed against the CCL20 molecule abolished this migration completely, suggesting that CCL20 is the main attracting factor for LCps, which is produced by the vaginal cells. These data indicate that vaginal epithelial cells play an important role in the immunological defense by attracting immune cells to the site of epithelial/pathogen contact.

journal_name

J Leukoc Biol

authors

Cremel M,Berlier W,Hamzeh H,Cognasse F,Lawrence P,Genin C,Bernengo JC,Lambert C,Dieu-Nosjean MC,Delézay O

doi

10.1189/jlb.0305147

subject

Has Abstract

pub_date

2005-07-01 00:00:00

pages

158-66

issue

1

eissn

0741-5400

issn

1938-3673

pii

jlb.0305147

journal_volume

78

pub_type

杂志文章
  • Tristetraprolin (TTP) coordinately regulates primary and secondary cellular responses to proinflammatory stimuli.

    abstract::TTP is an anti-inflammatory protein that acts by binding to AREs in its target mRNAs, such as Tnf mRNA, and promoting their deadenylation and decay. TNF released from inflammatory cells can then stimulate gene expression in tissue cells, such as fibroblasts. To determine whether TTP could affect the decay of TNF-induc...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.3A0214-106R

    authors: Qiu LQ,Lai WS,Bradbury A,Zeldin DC,Blackshear PJ

    更新日期:2015-04-01 00:00:00

  • Porcine NK cells display features associated with antigen-presenting cells.

    abstract::NK cells are members of the innate immunity and play a central role in the defense against viral infections and cancer development, but also contribute to triggering and shaping adaptive immune responses. Human NK cells may express MHC II and costimulatory molecules, including CD86, CD80, and OX40 ligand, which allows...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/JLB.4A0417-163RR

    authors: De Pelsmaeker S,Devriendt B,Leclercq G,Favoreel HW

    更新日期:2018-01-01 00:00:00

  • Epinephrine attenuates down-regulation of monocyte tumor necrosis factor receptors during human endotoxemia.

    abstract::Epinephrine inhibits lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF) production by increasing intracellular cAMP concentrations. Because agents that increase cAMP levels can enhance TNF receptor expression in vitro, granulocyte and monocyte TNF receptors were determined by FACS-analysis in 7 normal humans...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.61.2.156

    authors: van der Poll T,Calvano SE,Kumar A,Coyle SM,Lowry SF

    更新日期:1997-02-01 00:00:00

  • Genetic engineering of dendritic cells to express immunosuppressive molecules (viral IL-10, TGF-beta, and CTLA4Ig).

    abstract::There is growing evidence that, in addition to their role as initiators of immune responses, dendritic cells (DC) can exhibit tolerogenic properties. Immature DC deficient in cell surface costimulatory/accessory molecules can prolong organ and pancreatic islet allograft survival, whereas in vitro manipulation of DC by...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审

    doi:10.1002/jlb.66.2.293

    authors: Lu L,Lee WC,Takayama T,Qian S,Gambotto A,Robbins PD,Thomson AW

    更新日期:1999-08-01 00:00:00

  • Changes in subcellular localization and surface expression of L-selectin, alkaline phosphatase, and Mac-1 in human neutrophils during stimulation with inflammatory mediators.

    abstract::The localization of the adhesion protein L-selectin in human neutrophils was determined by subcellular fractionation and immunoelectron microscopy and compared with the localization of Mac-1 (alpha m beta 2) and alkaline phosphatase, the marker for secretory vesicles. L-selectin was found to be localized exclusively o...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.56.1.80

    authors: Borregaard N,Kjeldsen L,Sengeløv H,Diamond MS,Springer TA,Anderson HC,Kishimoto TK,Bainton DF

    更新日期:1994-07-01 00:00:00

  • Orphan nuclear receptors in T lymphocyte development.

    abstract::Lymphocyte development is initiated from hematopoietic stem cells and can be divided into multiple phenotypically distinct stages. Transcription factors play important roles in programming the developmental process of lymphocytes. Recent studies have identified key roles of several orphan nuclear receptors in T lympho...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审

    doi:

    authors: He YW

    更新日期:2002-09-01 00:00:00

  • Histone H4 directly stimulates neutrophil activation through membrane permeabilization.

    abstract::Extracellular histones have been implicated as a cause of tissue inflammatory injury in a variety of disorders including sepsis, lung, and liver diseases. However, little is known about their interactions with neutrophils and how this might contribute to injury. Here, it is shown that histone H4 acts as neutrophil act...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/JLB.3A0620-342R

    authors: Hsieh IN,Deluna X,White MR,Hartshorn KL

    更新日期:2020-08-17 00:00:00

  • Human neutrophils secrete vascular endothelial growth factor.

    abstract::Vascular endothelial growth factor (VEGF) is a multifunctional cytokine that plays a pivotal role in mediating neovascularization as well as other endothelial cell alterations during inflammation. In this study, we demonstrate that human neutrophils are a source of VEGF. We observed that isolated blood neutrophils rel...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.62.3.397

    authors: Taichman NS,Young S,Cruchley AT,Taylor P,Paleolog E

    更新日期:1997-09-01 00:00:00

  • Cellular metabolism of myeloid cells in sepsis.

    abstract::In recent years, it has become appreciated that immune cells have different metabolic profiles depending on their activation status. During sepsis, circulating leukocytes go through a hyperinflammatory state, which can be accompanied or followed by defective antimicrobial defenses (also described as immune tolerance o...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审

    doi:10.1189/jlb.4MR0216-066R

    authors: Arts RJ,Gresnigt MS,Joosten LA,Netea MG

    更新日期:2017-01-01 00:00:00

  • Homologous recombination into the eosinophil peroxidase locus generates a strain of mice expressing Cre recombinase exclusively in eosinophils.

    abstract::Eosinophils are generally linked to innate host defense against helminths, as well as the pathologies associated with allergic diseases, such as asthma. Nonetheless, the activities of eosinophils remain poorly understood, which in turn, has prevented detailed definitions of their role(s) in health and disease. Homolog...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0213089

    authors: Doyle AD,Jacobsen EA,Ochkur SI,Willetts L,Shim K,Neely J,Kloeber J,Lesuer WE,Pero RS,Lacy P,Moqbel R,Lee NA,Lee JJ

    更新日期:2013-07-01 00:00:00

  • Integrin-mediated epithelial-T cell interaction enhances nitric oxide production and increased intracellular inhibition of Chlamydia.

    abstract::T cell-mediated immunity against Chlamydia in mice is mediated at least in part by T cell-derived interferon-gamma (IFN-gamma) induction of the nitric oxide synthase (iNOS) system in infected epithelial cells. Although IFN-gamma alone could stimulate nitric oxide (NO) production from epithelial cells and inhibit the i...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.59.5.656

    authors: Igietseme JU,Uriri IM,Hawkins R,Rank RG

    更新日期:1996-05-01 00:00:00

  • Neuroprotective activities of CD4+CD25+ regulatory T cells in an animal model of Parkinson's disease.

    abstract::Progressive loss of dopaminergic neurons in the substantia nigra pars compacta and their terminal connections in the striatum are central features in Parkinson's disease (PD). Emerging evidence supports the notion that microglia neuroinflammatory responses speed neurodegenerative events. We demonstrated previously tha...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0507296

    authors: Reynolds AD,Banerjee R,Liu J,Gendelman HE,Mosley RL

    更新日期:2007-11-01 00:00:00

  • Cross-talk between tumor cells and neutrophils through the Fas (APO-1, CD95)/FasL system: human glioma cells enhance cell viability and stimulate cytokine production in neutrophils.

    abstract::Many tumor cells are resistant to Fas-mediated killing, which has been primarily used as a mechanism to evade immune attack. In this study, we found a new action of Fas on tumors where activation of the Fas signal may force tumor cells to produce survival factors for neutrophils. Human peripheral circulating neutrophi...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0702375

    authors: Hor WS,Huang WL,Lin YS,Yang BC

    更新日期:2003-03-01 00:00:00

  • IRAK4 activity controls immune responses to intracellular bacteria Listeria monocytogenes and Mycobacterium smegmatis.

    abstract::IL-1 receptor-associated kinase (IRAK) 4 is a central enzyme of the TLR pathways. This study tested the hypothesis that IRAK4 kinase activity is prerequisite for regulating innate immunity during infections with intracellular bacteria. To this end, we analyzed responses of macrophages obtained from mice expressing wil...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/JLB.2A1117-449R

    authors: Pattabiraman G,Murphy M,Agliano F,Karlinsey K,Medvedev AE

    更新日期:2018-10-01 00:00:00

  • Tumor growth increases Ia- macrophage synthesis of tumor necrosis factor-alpha and prostaglandin E2: changes in macrophage suppressor activity.

    abstract::Although tumor growth enhances macrophage (m phi) cytotoxic activity by increasing their tumor necrosis factor-alpha (TNF-alpha) production, increased prostaglandin E2 (PGE2) synthesis reduces most immune responses during tumor growth. Macrophages that do not express major histocompatibility complex class II molecules...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.53.5.550

    authors: Alleva DG,Burger CJ,Elgert KD

    更新日期:1993-05-01 00:00:00

  • Transcriptional profiling of human monocytes reveals complex changes in the expression pattern of inflammation-related genes in response to the annexin A1-derived peptide Ac1-25.

    abstract::Annexin A1 is a glucocorticoid-regulated, anti-inflammatory protein, which plays an important role as an endogenous regulator of the inflammatory response. Many of these anti-inflammatory properties are retained in the N-terminal annexin A1 peptide Ac1-25, which is released from the full-length protein by a neutrophil...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0307158

    authors: Lange C,Starrett DJ,Goetsch J,Gerke V,Rescher U

    更新日期:2007-12-01 00:00:00

  • Assessment of antibody-dependent respiratory burst activity from mouse neutrophils on Plasmodium yoelii malaria challenge outcome.

    abstract::New tools are required to expedite the development of an effective vaccine against the blood-stage infection with the human malaria parasite Plasmodium falciparum. This work describes the assessment of the ADRB assay in a mouse model, characterizing the functional interaction between antimalarial serum antibodies and ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0513274

    authors: Llewellyn D,de Cassan SC,Williams AR,Douglas AD,Forbes EK,Adame-Gallegos JR,Shi J,Pleass RJ,Draper SJ

    更新日期:2014-02-01 00:00:00

  • Mycoplasma stimulates the production of oxidative radicals by murine peritoneal macrophages.

    abstract::Mycoplasmas and mycoplasma membranes have been shown to induce the production of inflammatory cytokines, including tumor necrosis factor alpha and interleukin-6, as well as nitric oxide, by mouse macrophages and rat brain astrocytes. Luminol-enhanced chemiluminescence was used as a sensitive method to show that Mycopl...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.57.2.264

    authors: Avron A,Gallily R

    更新日期:1995-02-01 00:00:00

  • Expression of human NDRG2 by myeloid dendritic cells inhibits down-regulation of activated leukocyte cell adhesion molecule (ALCAM) and contributes to maintenance of T cell stimulatory activity.

    abstract::We reported previously that N-myc downstream-regulated gene 2 (NDRG2), a member of a new family of differentiation-related genes, is expressed specifically in dendritic cells (DC) differentiated from monocytes, CD34(+) progenitor cells, and the myelomonocytic leukemic cell line. In this study, we demonstrate that NDRG...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0507300

    authors: Choi SC,Kim KD,Kim JT,Kim JW,Lee HG,Kim JM,Jang YS,Yoon DY,Kim KI,Yang Y,Cho DH,Lim JS

    更新日期:2008-01-01 00:00:00

  • Actin cytoskeletal dynamics in T lymphocyte activation and migration.

    abstract::Dynamic rearrangements of the actin cytoskeleton are crucial for the function of numerous cellular elements including T lymphocytes. They are required for migration of T lymphocytes through the body to scan for the presence of antigens, as well as for the formation and stabilization of the immunological synapse at the...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审

    doi:10.1189/jlb.0602272

    authors: Samstag Y,Eibert SM,Klemke M,Wabnitz GH

    更新日期:2003-01-01 00:00:00

  • Technical advance: Langerhans cells derived from a human cell line in a full-thickness skin equivalent undergo allergen-induced maturation and migration.

    abstract::In this report, the construction of a functional, immunocompetent, full-thickness skin equivalent (SE) is described, consisting of an epidermal compartment containing keratinocytes, melanocytes, and human LCs derived from the MUTZ-3 cell line (MUTZ-LC) and a fibroblast-populated dermal compartment. The CD1a(+)Langerin...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0610374

    authors: Ouwehand K,Spiekstra SW,Waaijman T,Scheper RJ,de Gruijl TD,Gibbs S

    更新日期:2011-11-01 00:00:00

  • Bleomycin binding sites on alveolar macrophages.

    abstract::Previous work has demonstrated that bleomycin can directly stimulate alveolar macrophage secretion of fibroblast growth factors and monocyte chemotactic factors. In this study, rat alveolar macrophages obtained by bronchoalveolar lavage were examined for the presence of bleomycin binding sites, which might mediate thi...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.48.6.519

    authors: Denholm EM,Phan SH

    更新日期:1990-12-01 00:00:00

  • Actin polymerization contributes to neutrophil chemotactic dysfunction following thermal injury.

    abstract::The agent(s) and mechanism(s) responsible for suppression of neutrophil chemotaxis in association with major thermal injury have not been identified. We have proposed that the reduced random motility characterizing patients' cells may contribute to their generalized chemotactic dysfunction. Here we report that actin p...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.52.5.495

    authors: Hasslen SR,Ahrenholz DH,Solem LD,Nelson RD

    更新日期:1992-11-01 00:00:00

  • Expression of both types of human interleukin-8 receptors on mature neutrophils, monocytes, and natural killer cells.

    abstract::cDNA cloning revealed the presence of two related but distinct types of human interleukin-8 (IL-8) receptors, type I (type A) and type II (type B). By immunizing rabbits with glutathione-S-transferase fused with the NH2-terminal domain of each type of IL-8 receptor, we prepared polyclonal antibodies that specifically ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.57.1.180

    authors: Morohashi H,Miyawaki T,Nomura H,Kuno K,Murakami S,Matsushima K,Mukaida N

    更新日期:1995-01-01 00:00:00

  • Human monocyte-mediated cytotoxicity against herpes simplex virus-infected cells: activation of cytotoxic monocytes by free and liposome-encapsulated lymphokines.

    abstract::Human peripheral blood monocytes were incubated with free or liposome-encapsulated human lymphokines containing macrophage-activating factor (MAF) and tested for their effect on herpes simplex virus (HSV)-infected target cells. Activated monocytes lysed allogeneic HSV type 2 (HSV-2)-infected whole human embryo cells a...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.37.4.461

    authors: Koff WC,Showalter SD,Chakrabarty MK,Hampar B,Ceccorulli LM,Kleinerman ES

    更新日期:1985-04-01 00:00:00

  • Aloe emodin relieves Ang II-induced endothelial junction dysfunction via promoting ubiquitination mediated NLRP3 inflammasome inactivation.

    abstract::Recent studies have revealed that aloe emodin (AE), a natural compound from the root and rhizome of Rheum palmatum L., exhibits significant pharmacologic activities. However, the pharmacologic relevance of the compound, particularly for cardiovascular disease, remains largely unknown. Here, we hypothesized that AE cou...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/JLB.3MA0520-582R

    authors: Zhang Y,Song Z,Huang S,Zhu L,Liu T,Shu H,Wang L,Huang Y,Chen Y

    更新日期:2020-12-01 00:00:00

  • Osteopontin augments CD3-mediated interferon-gamma and CD40 ligand expression by T cells, which results in IL-12 production from peripheral blood mononuclear cells.

    abstract::Osteopontin is an RGD-containing bone matrix protein with cytokine-like functions that is associated with early stages of Th1-mediated diseases. Although the function of osteopontin in these responses is unknown, it is expressed by activated T cells and macrophages and can costimulate T cell proliferation. Studies hav...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:

    authors: O'Regan AW,Hayden JM,Berman JS

    更新日期:2000-10-01 00:00:00

  • IL-17 superfamily cytokines modulate normal germinal center B cell migration.

    abstract::The germinal center (GC) is a dynamic structure formed by proliferating B cells in the follicles of secondary lymphoid organs during T cell-dependent antibody responses to exogenous antigens. GC is composed by a dark zone, enriched in proliferating centroblasts (CBs), and a light zone where CBs migrate and transform i...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审

    doi:10.1189/jlb.1VMR0216-096RR

    authors: Ferretti E,Ponzoni M,Doglioni C,Pistoia V

    更新日期:2016-11-01 00:00:00

  • Programming and memory dynamics of innate leukocytes during tissue homeostasis and inflammation.

    abstract::The field of innate immunity is witnessing a paradigm shift regarding "memory" and "programming" dynamics. Past studies of innate leukocytes characterized them as first responders to danger signals with no memory. However, recent findings suggest that innate leukocytes, such as monocytes and neutrophils, are capable o...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审

    doi:10.1189/jlb.6MR0117-027RR

    authors: Lee C,Geng S,Zhang Y,Rahtes A,Li L

    更新日期:2017-09-01 00:00:00

  • IL-10 receptor dysfunction in macrophages during chronic inflammation.

    abstract::The immunosuppressive activity of interleukin-10 (IL-10) makes this cytokine a potentially important clinical tool to reduce inflammatory responses in various diseases. Its efficacy as a therapeutic modality is dependent on the responsiveness of immune cells. We report that macrophages from mice chronically infected w...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:

    authors: Avdiushko R,Hongo D,Lake-Bullock H,Kaplan A,Cohen D

    更新日期:2001-10-01 00:00:00