Characterization of macrophage subsets regulating murine natural killer cell activity.


:We examined the relationship of I-A expression by normal murine macrophages to their immunoregulatory role on natural killer cell activity. Macrophages were isolated on the basis of plastic adherence; characterized on the basis of conventional markers such as phagocytic ability, cytoplasmic non-specific esterase activity, surface MAC-1 and F4/80 antigen expression; and then used for functional studies relative to their expression of surface I-A. Two functional macrophage subsets were identified: NK-stimulatory and NK-suppressive subsets. The former function was associated with splenic macrophages, which were predominantly I-A+ as identified with a radioautographic immunolabeling technique; the latter function with peritoneal macrophages which were predominantly I-A-. Loss of macrophage I-A expression in vitro was delayed in the presence of indomethacin and enhanced in the presence of PGE2, indicating that PGE2 down-regulates I-A expression on macrophages. The NK stimulatory function of I-A+ macrophages was attributable to a soluble mediator, identified as IFN-gamma, since the stimulatory ability was abrogated with an anti-IFN-gamma antibody. I-A expression appears to be important for the stimulatory function, since some interference with this function was noted in the presence of anti-I-A antibody. The NK-suppressor function of I-A- macrophages was attributable to the soluble mediator PGE2, since this function was abrogated with indomethacin or anti-PGE2 antibody. These results are relevant to the understanding of normal in vivo immunoregulation by macrophages.


J Leukoc Biol


Nelson JA,Parhar RS,Scodras JM,Lala PK




Has Abstract


1990-11-01 00:00:00












  • Increased mortality, blunted production of nitric oxide, and increased production of TNF-alpha in endotoxemic TGF-beta1 transgenic mice.

    abstract::The expression of the inducible isoform of nitric oxide synthase (NOS2, iNOS) is increased in patients undergoing sepsis as well as in animal models in which septic shock is induced by injection of bacterial lipopolysaccharide (LPS). Transforming growth factor-beta1 (TGF-beta1) potently suppresses NO production both i...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Vodovotz Y,Kopp JB,Takeguchi H,Shrivastav S,Coffin D,Lucia MS,Mitchell JB,Webber R,Letterio J,Wink D,Roberts AB

    更新日期:1998-01-01 00:00:00

  • Endogenous monocyte chemoattractant protein-1 recruits monocytes in the zymosan peritonitis model.

    abstract::The role of monocyte chemoattractant protein-1 (MCP-1) in the recruitment of blood-derived monocytes in a model of zymosan peritoneal inflammation was investigated. After zymosan injection (1 mg) a rapid influx of polymorphonuclear leukocytes (PMN) and monocytes into the peritoneal cavity associated with mouse MCP-1 (...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Ajuebor MN,Flower RJ,Hannon R,Christie M,Bowers K,Verity A,Perretti M

    更新日期:1998-01-01 00:00:00

  • The RP105/MD-1 complex: molecular signaling mechanisms and pathophysiological implications.

    abstract::Radioprotective 105 kDa (CD180) is an unusual TLR that lacks an intracellular Toll-IL-1R signaling domain and exhibits unconventional homodimerization behavior. Differential expression and functions of radioprotective 105 kDa have been associated with immune-mediated pathologies, including infection, chronic inflammat...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审


    authors: Schultz TE,Blumenthal A

    更新日期:2017-01-01 00:00:00

  • Identification of glyceraldehyde-3-phosphate dehydrogenase as a Ca2+-dependent fusogen in human neutrophil cytosol.

    abstract::The membrane fusion events observed during neutrophil degranulation are important aspects of the immunoregulatory system. In an attempt to understand the regulation of granule-plasma membrane fusion, we have begun characterizing human neutrophil cytosol for fusion activity, finding that 50% of the fusogenic activity c...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Hessler RJ,Blackwood RA,Brock TG,Francis JW,Harsh DM,Smolen JE

    更新日期:1998-03-01 00:00:00

  • Involvement of CD11b/CD18 in enhanced neutrophil adhesion by Fc gamma receptor stimulation.

    abstract::Neutrophils showed a rapid and transient adhesion to immunoglobulin G (IgG)-coated plates compared with their adhesion to bovine serum albumin (BSA)-coated plates: the adhesion reached a peak after 15 min of incubation and then gradually returned to almost the basal state in 60 min. The addition of monomeric IgG or an...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Kusunoki T,Tsuruta S,Higashi H,Hosoi S,Hata D,Sugie K,Mayumi M,Mikawa H

    更新日期:1994-06-01 00:00:00

  • Extracellular adenosine enhances the ability of PMNs to kill Streptococcus pneumoniae by inhibiting IL-10 production.

    abstract::Polymorphonuclear leukocytes (PMNs) are crucial for initial control of Streptococcus pneumoniae (pneumococcus) lung infection; however, as the infection progresses their persistence in the lungs becomes detrimental. Here we explored why the antimicrobial efficacy of PMNs declines over the course of infection. We found...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Siwapornchai N,Lee JN,Tchalla EYI,Bhalla M,Yeoh JH,Roggensack SE,Leong JM,Bou Ghanem EN

    更新日期:2020-09-01 00:00:00

  • CSF-1, IGF-1, and the control of postnatal growth and development.

    abstract::Growth hormone controls somatic growth in mammals by regulating the production of IGF-1, which is predominantly made by the liver. The development of cells within the MPS is controlled by the lineage-specific growth factor M-CSF (CSF-1). In this review, we summarize the role of CSF-1-dependent macrophages in somatic g...

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    pub_type: 杂志文章,评审


    authors: Gow DJ,Sester DP,Hume DA

    更新日期:2010-09-01 00:00:00

  • Innate immune cell networking in hepatitis C virus infection.

    abstract::Persistent viral infection, such as HCV infection, is the result of the inability of the host immune system to mount a successful antiviral response, as well as the escape strategies devised by the virus. Although each individual component of the host immune system plays important roles in antiviral immunity, the inte...

    journal_title:Journal of leukocyte biology

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    authors: Saha B,Szabo G

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  • Regulation of herpes simplex virus type 1 gene expression in nonpermissive murine resident peritoneal macrophages.

    abstract::The restriction of herpes simplex virus type 1 (HSV-1) gene expression in nonpermissive murine resident peritoneal macrophages (ResPMO) was analyzed at the level of transcription. Using nuclear run-on assays, we established that transcription of a representative gene from each HSV-1 kinetic class was initiated in both...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Wu L,Morahan PS,Leary K

    更新日期:1993-01-01 00:00:00

  • Metabolic energy sensors as targets for designing host-directed therapies for tuberculosis.

    abstract::A wealth of scientific and clinical evidence during the past few years has lent credence to the idea that key components of the host immune effector mechanisms can be targeted to boost current tuberculosis (TB) treatment and control patient relapse. These host-directed strategies not only accelerate the clearance of p...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审


    authors: Cheng CY,Böhme J,Singhal A

    更新日期:2018-02-01 00:00:00

  • Neutrophil oxidative metabolism and killing of P. brasiliensis after air pouch infection of susceptible and resistant mice.

    abstract::The oxidative burst of polymorphonuclear neutrophils (PMN) and their ability to inhibit Paracoccidioides brasiliensis growth was studied in susceptible (B10.A) and resistant (A/J) mice. The cells were obtained after subcutaneous inoculation in air pouches, yielding highly pure PMN preparations; the number of cells was...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Meloni-Bruneri LH,Campa A,Abdalla DS,Calich VL,Lenzi HL,Burger E

    更新日期:1996-04-01 00:00:00

  • Recycling endosome-dependent and -independent mechanisms for IL-10 secretion in LPS-activated macrophages.

    abstract::IL-10 is a key anti-inflammatory cytokine secreted by activated macrophages as a feedback control mechanism to prevent excessive inflammatory responses. Here, we define multiple intracellular trafficking pathways involved in the secretion of newly synthesized IL-10 from macrophages following TLR4 activation with LPS, ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Stanley AC,Lieu ZZ,Wall AA,Venturato J,Khromykh T,Hamilton NA,Gleeson PA,Stow JL

    更新日期:2012-12-01 00:00:00

  • The short and happy life of neutrophil activation.

    abstract::This is largely a history of attempts from approximately 1960 until about 1980-81 to understand the mechanisms by which neutrophils are activated by chemotactic factors to induce chemotaxis and granule secretion. As such, it deals with the growth of our knowledge of neutrophil chemotactic factors and their receptors; ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审


    authors: Becker EL

    更新日期:1990-04-01 00:00:00

  • Phosphatidylinositol 3-kinase inhibitors prevent mouse cytotoxic T-cell development in vitro.

    abstract::To become competent killer cells, CD8(+) T cells require stimulation through signal transduction pathways associated with the T-cell receptor, costimulatory molecules such as CD28, and cytokine receptors such as the interleukin (IL)-2 receptor. We used wortmannin and LY294002, two inhibitors of phosphatidylinositol 3-...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Phu T,Haeryfar SM,Musgrave BL,Hoskin DW

    更新日期:2001-05-01 00:00:00

  • Endotoxin tolerance attenuates LPS-induced TLR4 mobilization to lipid rafts: a condition reversed by PKC activation.

    abstract::Endotoxin tolerance is characterized by attenuated macrophage activation to subsequent LPS challenge and can be reversed through nonspecific protein kinase C (PKC) activation, and activation by LPS within naïve cells requires the activation of the cell surface receptors CD14 and TLR4 on lipid rafts. The effect of PKC ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Cuschieri J,Billigren J,Maier RV

    更新日期:2006-12-01 00:00:00

  • Anti-HIV-1 immunotoxin 3B3(Fv)-PE38: enhanced potency against clinical isolates in human PBMCs and macrophages, and negligible hepatotoxicity in macaques.

    abstract::Highly active antiretroviral therapy (HAART) against human immunodeficiency virus type 1 (HIV-1) infection dramatically suppresses viral load, leading to marked reductions in HIV-1 associated morbidity and mortality. However, infected cell reservoirs and low-level replication persist in the face of suppressive HAART, ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Kennedy PE,Bera TK,Wang QC,Gallo M,Wagner W,Lewis MG,Berger EA,Pastan I

    更新日期:2006-11-01 00:00:00

  • Leukocyte subsets and neutrophil function after short-term spaceflight.

    abstract::Changes in leukocyte subpopulations and function after spaceflight have been observed but the mechanisms underlying these changes are not well defined. This study investigated the effects of short-term spaceflight (8-15 days) on circulating leukocyte subsets, stress hormones, immunoglobulin levels, and neutrophil func...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Stowe RP,Sams CF,Mehta SK,Kaur I,Jones ML,Feeback DL,Pierson DL

    更新日期:1999-02-01 00:00:00

  • Cellular metabolism of myeloid cells in sepsis.

    abstract::In recent years, it has become appreciated that immune cells have different metabolic profiles depending on their activation status. During sepsis, circulating leukocytes go through a hyperinflammatory state, which can be accompanied or followed by defective antimicrobial defenses (also described as immune tolerance o...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审


    authors: Arts RJ,Gresnigt MS,Joosten LA,Netea MG

    更新日期:2017-01-01 00:00:00

  • The strain of mouse and assay conditions influence whether MuIFN-gamma primes or activates macrophages for tumor cell killing.

    abstract::Investigators from two different laboratories have compared several variables in the short-term macrophage-mediated cytotoxicity assays used by each group to study the role of MuIFN-gamma in macrophage activation. The findings suggest that the capacity of MuIFN-gamma to activate macrophages without the need for a seco...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Pace JL,Varesio L,Russell SW,Blasi E

    更新日期:1985-04-01 00:00:00

  • Macrophage-like tumor cells as tools to study chemoattractive activity.

    abstract::Macrophage-like tumor cells can be obtained in large quantities as rather homogeneous populations, making these cells useful for chemotaxis assays. Therefore, macrophage-like cells J774A, WEHI-3, P388D1, IC-21, and NCTC 1469, all of murine origin, and U937 of human origin, were tested for chemotactic activity to a num...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Terheggen P,Van Loveren H,Den Otter W

    更新日期:1985-12-01 00:00:00

  • Infection of a murine T-cell line with a retrovirus carrying c-myc decreases the interleukin-2 cell dependence by a nonautocrine mechanism.

    abstract::A murine interleukin-2 (IL-2)-dependent T-cell line, CTLL-2, was infected with a retrovirus carrying the mouse c-myc cDNA and the Tn-5 neogene. Transduced cells were selected in the presence of Geneticin sulfate (G418); these cells were shown to express both the endogenous and the transduced c-myc genes. The IL-2 requ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Shapiro IM,Winberg G,Stefanescu I,Wigzell H,Menezes J

    更新日期:1988-12-01 00:00:00

  • Decidual macrophages: key regulators of vascular remodeling in human pregnancy.

    abstract::Successful remodeling of the uterine spiral arteries is essential for a complication-free pregnancy and is best described in terms of its morphologic features. The molecular mediators and cellular sources of spiral artery remodeling are not known, although a role for uterine leukocytes has been proposed. Immunohistoch...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Lash GE,Pitman H,Morgan HL,Innes BA,Agwu CN,Bulmer JN

    更新日期:2016-08-01 00:00:00

  • Novel insights into the inhibitory effects of Galectin-1 on neutrophil recruitment under flow.

    abstract::Galectin-1 (Gal-1) is a beta-galactoside-binding protein endowed with anti-inflammatory properties. The purpose of this study was to investigate the effects of endogenous and exogenous Gal-1 on neutrophil recruitment onto TNF-treated endothelium. The effect of human recombinant (hr)Gal-1 on markers of neutrophil activ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Cooper D,Norling LV,Perretti M

    更新日期:2008-06-01 00:00:00

  • ICAM-1: A master regulator of cellular responses in inflammation, injury resolution, and tumorigenesis.

    abstract::ICAM-1 is a cell surface glycoprotein and an adhesion receptor that is best known for regulating leukocyte recruitment from circulation to sites of inflammation. However, in addition to vascular endothelial cells, ICAM-1 expression is also robustly induced on epithelial and immune cells in response to inflammatory sti...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审


    authors: Bui TM,Wiesolek HL,Sumagin R

    更新日期:2020-09-01 00:00:00

  • Hypoxemia modifies circulating and exudate neutrophil number and functional responses in carrageenin-induced pleurisy in the rat.

    abstract::To assess the effect of hypoxemia on the responses of polymorphonuclear neutrophils (PMN) during an inflammatory response, rats were maintained in a low F1O2 atmosphere (9% O2) or room air for 12 h before intrathoracic injection of carrageenin or intradermal injections of agonists. After 4 h, hypoxemic rats had 50% mo...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Tremblay PB,Macari DM,Martel D,du Souich P,Barja-Fidalgo C,Marleau S

    更新日期:2000-06-01 00:00:00

  • Selective expansion of perforin-positive CD8+ T cells by immature dendritic cells infected with live Bacillus Calmette-Guérin mycobacteria.

    abstract::Live, but not dead Bacillus Calmette-Guérin (BCG) is partially protective against infection by Mycobacterium tuberculosis, which causes a disease with high mortality in immune compromised individuals. We have shown that uptake of BCG induces maturation of immature dendritic cells (DCs) regardless of the viability of t...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Tsunetsugu-Yokota Y,Tamura H,Tachibana M,Ogata K,Honda M,Takemori T

    更新日期:2002-07-01 00:00:00

  • Increase in the rat blood leukocyte counts induced by PAF-acether is suppressed by general anesthesia.

    abstract::Blood leukocyte count alterations induced by PAF-acether in anesthetized and nonanesthetized rats were investigated. Intravenous injection of increasing amounts of PAF-acether (1.5-8 micrograms/kg) in nonanesthetized animals induced dose-dependent hemoconcentration and leukocytosis. The former was apparent within 10 m...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Bozza PT,Silva PM,Castro-Faria-Neto HC,Martins MA,Cordeiro RS

    更新日期:1992-02-01 00:00:00

  • A Cys-Gly-Cys triad in the dehydrogenase region of Nox2 plays a key role in the interaction with p67phox.

    abstract::p67(phox) is the paramount cytosolic regulator of the superoxide-generating Nox of phagocytes, by controlling the conformation of the catalytic component, Nox2. The initiating event of this process is a protein-protein interaction between p67(phox) and the part of Nox2 protruding into the cytosol, known as the dehydro...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Dahan I,Smith SM,Pick E

    更新日期:2015-11-01 00:00:00

  • TLR9 is required for MAPK/NF-κB activation but does not cooperate with TLR2 or TLR6 to induce host resistance to Brucella abortus.

    abstract::Brucella abortus is a Gram-negative intracellular bacterial pathogen that causes a zoonosis of worldwide occurrence, leading to undulant fever in humans and abortion in domestic animals. B. abortus is recognized by several pattern-recognition receptors triggering pathways during the host innate immune response. Theref...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Gomes MT,Campos PC,Pereira Gde S,Bartholomeu DC,Splitter G,Oliveira SC

    更新日期:2016-05-01 00:00:00

  • L-selectin and intercellular adhesion molecule-1 regulate the development of Concanavalin A-induced liver injury.

    abstract::Concanavalin A (Con A)-induced hepatitis is a model for human T cell-mediated hepatitis. We evaluated the role of L-selectin and intercellular adhesion molecule-1 (ICAM-1) in this model by injecting Con A intravenously in mice lacking L-selectin (L-selectin-/-), ICAM-1 (ICAM-1-/-), or both (L-selectin/ICAM-1-/-). Bloo...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Kawasuji A,Hasegawa M,Horikawa M,Fujita T,Matsushita Y,Matsushita T,Fujimoto M,Steeber DA,Tedder TF,Takehara K,Sato S

    更新日期:2006-04-01 00:00:00