The THC-induced suppression of Th1 polarization in response to Legionella pneumophila infection is not mediated by increases in corticosterone and PGE2.


:T helper cell type 1 (Th1)-polarizing cytokines are induced by Legionella pneumophila infection and are suppressed by pretreatment with marijuana cannabinoids (CB). Glucocorticoids and prostaglandin E2(PGE2) are also reported to suppress Th1 polarization and are induced by Delta9-tetrahydrocannabinol (THC), so their role in the suppression of polarizing cytokines was examined. Injection of L. pneumophila or THC alone into BALB/c mice induced a rapid and transient rise in serum corticosterone (CS), and the injection of both agents significantly augmented the CS response, demonstrating that THC increased CS in Legionella-infected mice. Pretreatment with the CB receptor 1 (CB1) antagonist SR141716A had no effect on the THC-induced CS response, but CB2 antagonist (SR144528) treatment increased the CS response. To see if increased CS contributed to the down-regulation of Th1 cytokines, mice were pretreated with the steroid antagonist RU486 before THC injection and Legionella infection. The results showed that RU486 did not attenuate the THC-induced suppression of serum interleukin (IL)-12 or interferon-gamma (IFN-gamma). In addition to CS, THC injection increased urinary PGE2 metabolites, and the CB1 antagonist attenuated this increase. Although L. pneumophila infection increased urinary PGE2, THC pretreatment did not enhance this response; in addition, treatment with the cyclooxygenase inhibitor, indomethacin, did not block the THC-induced suppression of IL-12 and IFN-gamma. These results suggest that the elevation of CS and PGE2 does not account for the THC-induced attenuation of the Th1 cytokine response, and it is concluded that other suppressive mediators are induced by THC or that the drug acts directly on immune cells to suppress cytokine production.


J Leukoc Biol


Newton CA,Lu T,Nazian SJ,Perkins I,Friedman H,Klein TW




Has Abstract


2004-10-01 00:00:00














  • Influence of interleukin-2 infusion on cell cycle kinetics in the Walker-256 carcinosarcoma.

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Wan JM,Bistrian BR,Figoni MA,Istfan NW

    更新日期:1994-02-01 00:00:00

  • Interleukin-6 suppression of neutrophil apoptosis is neutrophil concentration dependent.

    abstract::Apoptosis of polymorphonuclear leukocytes (PMNs) is a critical step in the resolution of tissue inflammation. PMN apoptosis has been studied extensively in vitro, and diverse inflammatory mediators have been shown to modulate the process. The reported effects of interleukin-6 (IL-6) on PMN apoptosis are inconsistent; ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Biffl WL,Moore EE,Moore FA,Barnett CC Jr

    更新日期:1995-11-01 00:00:00

  • Heterogeneity in composition of mouse uterine natural killer cell granules.

    abstract::uNK cells differ from cNK cells, as they produce angiogenic molecules critical for normal implantation site development. We evaluated heterogeneity among DBA(+)uNK cells for Prf, Gzma, and Vegfa. Ctsd and Srgn expression was used to assign intracellular sorting of these molecules on gd7, -9, and -14. Vegfa was present...

    journal_title:Journal of leukocyte biology

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    authors: Lima PD,Croy BA,Degaki KY,Tayade C,Yamada AT

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  • Role of IFN-gamma on dissociation between nitric oxide and TNF/IL-6 production by murine peritoneal cells after restimulation with bacterial lipopolysaccharide.

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Tominaga K,Saito S,Matsuura M,Funatogawa K,Matsumura H,Nakano M

    更新日期:1999-12-01 00:00:00

  • Activation of EphA receptors on CD4+CD45RO+ memory cells stimulates migration.

    abstract::We have demonstrated previously that binding of ephrin-A1 to EphA receptors on human CD4(+) and CD8(+) T cells stimulates migration. Two EphA receptors have been reported in T cells: EphA1 at the protein level and EphA4 at the mRNA level. In this study, we wanted to investigate the expression profile of these receptor...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Holen HL,Nustad K,Aasheim HC

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  • RLR-mediated production of interferon-β by a human dendritic cell subset and its role in virus-specific immunity.

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Szabo A,Bene K,Gogolák P,Réthi B,Lányi Á,Jankovich I,Dezső B,Rajnavölgyi E

    更新日期:2012-07-01 00:00:00

  • Decidual macrophages: key regulators of vascular remodeling in human pregnancy.

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    authors: Lash GE,Pitman H,Morgan HL,Innes BA,Agwu CN,Bulmer JN

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  • Antibody-facilitated macrophage killing of Trypanosoma musculi is an extracellular process as studied in several variations of an in vitro analytical system.

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Albright JW,Stewart MJ,Latham PS,Albright JF

    更新日期:1994-11-01 00:00:00

  • Phosphatidylinositol 3-kinase inhibitors prevent mouse cytotoxic T-cell development in vitro.

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Phu T,Haeryfar SM,Musgrave BL,Hoskin DW

    更新日期:2001-05-01 00:00:00

  • Regulation of macrophage motility by Irgm1.

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    journal_title:Journal of leukocyte biology

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    authors: Henry SC,Traver M,Daniell X,Indaram M,Oliver T,Taylor GA

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  • TAK1 regulates multiple protein kinase cascades activated by bacterial lipopolysaccharide.

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Lee J,Mira-Arbibe L,Ulevitch RJ

    更新日期:2000-12-01 00:00:00

  • Nonspecific CD4(+) T cells with uptake of antigen-specific dendritic cell-released exosomes stimulate antigen-specific CD8(+) CTL responses and long-term T cell memory.

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Hao S,Yuan J,Xiang J

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  • Matrix metalloproteinase-9 promotes neutrophil and T cell recruitment and migration in the postischemic liver.

    abstract::Matrix metalloproteinases-2 and -9 (MMP-2/9) are critically involved in degradation of extracellular matrix, and their inhibition is discussed as a promising strategy against hepatic ischemia-reperfusion (I/R) injury. Here, we analyzed the role of MMP-2 and -9 for leukocyte migration and tissue injury in sham-operated...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Khandoga A,Kessler JS,Hanschen M,Khandoga AG,Burggraf D,Reichel C,Hamann GF,Enders G,Krombach F

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  • IL-27 renders DC immunosuppressive by induction of B7-H1.

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Karakhanova S,Bedke T,Enk AH,Mahnke K

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Feng Y,Huang N,Wu Q,Wang B

    更新日期:2005-11-01 00:00:00

  • CD71+ erythroid cells from neonates born to women with preterm labor regulate cytokine and cellular responses.

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Miller D,Romero R,Unkel R,Xu Y,Vadillo-Ortega F,Hassan SS,Gomez-Lopez N

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  • Characteristics of alveolar macrophages in experimental septic lung.

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    journal_title:Journal of leukocyte biology

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    authors: Goya T,Abe M,Shimura H,Torisu M

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审


    authors: Roebuck KA,Carpenter LR,Lakshminarayanan V,Page SM,Moy JN,Thomas LL

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  • Heat-shock protein-90 prolongs septic neutrophil survival by protecting c-Src kinase and caspase-8 from proteasomal degradation.

    abstract::The brief lifespan of the polymorphonuclear neutrophil (PMN) is regulated through its capacity to undergo apoptosis, a constitutive process that is actively inhibited during sepsis. We sought to define the cellular mechanisms through which Heat Shock Protein 90 (Hsp90) prolongs the survival of inflammatory PMN. We eva...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Gupta S,Lee CM,Wang JF,Parodo J,Jia SH,Hu J,Marshall JC

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  • Losartan, a selective inhibitor of subtype AT1 receptors for angiotensin II, inhibits neutrophil recruitment in the lung triggered by fMLP.

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    journal_title:Journal of leukocyte biology

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    authors: Raiden S,Pereyra Y,Nahmod V,Alvarez C,Castello L,Giordano M,Geffner J

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  • Inhibition of glucocorticoid-mediated, caspase-independent dendritic cell death by CD40 activation.

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Kim KD,Choe YK,Choe IS,Lim JS

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  • Phorbol ester-induced loss of membrane sialic acid: implications for tumor cytolysis by natural killer cells.

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Nabi ZF,Zucker-Franklin D,Baten A

    更新日期:1989-03-01 00:00:00

  • Nitric oxide post-transcriptionally up-regulates LPS-induced IL-8 expression through p38 MAPK activation.

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Ma P,Cui X,Wang S,Zhang J,Nishanian EV,Wang W,Wesley RA,Danner RL

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  • Synergism of NOD2 and NLRP3 activators promotes a unique transcriptional profile in murine dendritic cells.

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Conforti-Andreoni C,Beretta O,Licandro G,Qian HL,Urbano M,Vitulli F,Ricciardi-Castagnoli P,Mortellaro A

    更新日期:2010-12-01 00:00:00

  • Store-operated calcium entry mediates intracellular alkalinization, ERK1/2, and Akt/PKB phosphorylation in bovine neutrophils.

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Sandoval AJ,Riquelme JP,Carretta MD,Hancke JL,Hidalgo MA,Burgos RA

    更新日期:2007-11-01 00:00:00

  • Respiratory viral infection, epithelial cytokines, and innate lymphoid cells in asthma exacerbations.

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审


    authors: Kumar RK,Foster PS,Rosenberg HF

    更新日期:2014-09-01 00:00:00

  • Late IL-3-induced phenotypic and functional alterations in human basophils require continuous IL-3 receptor signaling.

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Kämpfer SS,Odermatt A,Dahinden CA,Fux M

    更新日期:2017-01-01 00:00:00

  • Cytokine requirements for the growth and development of mouse NK cells in vitro.

    abstract::Natural killer (NK) cells arise from immature progenitors present in fetal tissues and adult bone marrow, but the factors responsible for driving the proliferation and differentiation of these progenitors are poorly understood. Mouse NK cells had previously been thought not to express interleukin (IL)-2Ralpha chains, ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Toomey JA,Gays F,Foster D,Brooks CG

    更新日期:2003-08-01 00:00:00

  • Methods for measuring myeloperoxidase activity toward assessing inhibitor efficacy in living systems.

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审


    authors: Huang J,Milton A,Arnold RD,Huang H,Smith F,Panizzi JR,Panizzi P

    更新日期:2016-04-01 00:00:00