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Transformation of crustacean pathogenic bacterium Spiroplasma eriocheiris and expression of yellow fluorescent protein.

Abstract：

:Spiroplasma eriocheiris, the cause of crab trembling disease, is a wall-less bacterium, related to Mycoplasmas, measuring 2.0-10.0 μm long. It features a helical cell shape and a unique swimming mechanism that does not use flagella; instead, it moves by switching the cell helicity at a kink traveling from the front to the tail. S. eriocheiris seems to use a novel chemotactic system that is based on the frequency of reversal swimming behaviors rather than the conventional two-component system, which is generally essential for bacterial chemotaxis. To identify the genes involved in these novel mechanisms, we developed a transformation system by using oriC plasmid harboring the tetracycline resistant gene, tetM, which is under the control of a strong promoter for an abundant protein, elongation factor-Tu. The transformation efficiency achieved was 1.6 × 10-5 colony forming unit (CFU) for 1 μg DNA, enabling the expression of the enhanced yellow fluorescent protein (EYFP).

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Terahara N,Tulum I,Miyata M

doi

10.1016/j.bbrc.2017.03.144

subject

Has Abstract

pub_date

2017-06-03 00:00:00

pages

488-493

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(17)30620-4

journal_volume

487

pub_type

杂志文章

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