Abstract:
BACKGROUND:Cannabinoids are known to have an anti-tumorous effect, but the underlying mechanisms are only sparsely understood. Mechanical characteristics of tumor cells represent a promising marker to distinguish between tumor cells and the healthy tissue. We tested the hypothesis whether cannabinoids influence the tumor cell specific mechanical and migratory properties and if these factors are a prognostic marker for the invasiveness of tumor cells. METHODS:3 different glioblastoma cell lines were treated with cannabinoids and changes of mechanical and migratory properties of single cells were measured using atomic force microscopy and time lapse imaging. The invasiveness of cell lines was determined using a co-culture model with organotypic hippocampal slice cultures. RESULTS:We found that cannabinoids are capable of influencing migratory and mechanical properties in a cell line specific manner. A network analysis revealed a correlation between a "generalized stiffness" and the invasiveness for all tumor cell lines after 3 and 4 d of invasion time: r3d = -0.88 [-0.52;-0.97]; r4d = -0.90 [-0.59;-0.98]. CONCLUSIONS:Here we could show that a "generalized stiffness" is a profound marker for the invasiveness of a tumor cell population in our model and thus might be of high clinical relevance for drug testing. Additionally cannabinoids were shown to be of potential use for therapeutic approaches of glioblastoma.
journal_name
Cell Adh Migrjournal_title
Cell adhesion & migrationauthors
Hohmann T,Grabiec U,Ghadban C,Feese K,Dehghani Fdoi
10.1080/19336918.2016.1183867subject
Has Abstractpub_date
2017-01-02 00:00:00pages
54-67issue
1eissn
1933-6918issn
1933-6926journal_volume
11pub_type
杂志文章abstract::Familial dysautonomia (FD) is a hereditary neuronal disease characterized by poor development and progressive degeneration of the sensory and autonomic nervous system. Majority of FD (99.5%) results from a single nucleotide point mutation in the IKBKAP gene encoding IKAP, also known as elongation protein 1 (ELP1). The...
journal_title:Cell adhesion & migration
pub_type: 杂志文章
doi:10.4161/cam.2.4.6630
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abstract::Spinal cord injury (SCI) has been regarded clinically as an irreversible damage caused by tissue contusion due to a blunt external force. Past research had focused on the analysis of the pathogenesis of secondary injury that extends from the injury epicenter to the periphery, as well as tissue damage and neural cell d...
journal_title:Cell adhesion & migration
pub_type: 杂志文章,评审
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abstract::Many physiological and pathological processes involve tissue cells sensing the rigidity of their environment. In general, tissue cells have been shown to react to the stiffness of their environment by regulating their level of contractility, and in turn applying traction forces on their environment to probe it. This m...
journal_title:Cell adhesion & migration
pub_type: 杂志文章,评审
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abstract::It is well recognized that a number of proteins present within adhesion complexes perform discrete signaling functions outside these adhesion complexes, including transcriptional control. In this respect, β-catenin is a well-known example of an adhesion protein present both in cadherin complexes and in the nucleus wh...
journal_title:Cell adhesion & migration
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abstract::The stroma in human carcinomas consists of extracellular matrix and various types of non-carcinoma cells, mainly leukocytes, endothelial cells, fibroblasts, myofibroblasts and bone marrow-derived progenitors. The tumor-associated stroma actively supports tumor growth by stimulating neo-angiogenesis, as well as prolife...
journal_title:Cell adhesion & migration
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journal_title:Cell adhesion & migration
pub_type: 杂志文章,评审
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abstract::Genetically engineered, conditionally replicating herpes simplex viruses type 1 (HSV-1) are promising therapeutic agents for brain tumors and other solid cancers. They can replicate in situ, spread and exhibit oncolytic activity via a direct cytocidal effect. One of the advantages of HSV-1 is the capacity to incorpora...
journal_title:Cell adhesion & migration
pub_type: 杂志文章
doi:10.4161/cam.2.3.6353
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journal_title:Cell adhesion & migration
pub_type: 杂志文章,评审
doi:10.1080/19336918.2015.1098800
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journal_title:Cell adhesion & migration
pub_type: 杂志文章
doi:10.4161/cam.3.2.8207
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journal_title:Cell adhesion & migration
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journal_title:Cell adhesion & migration
pub_type: 杂志文章
doi:10.4161/cam.26941
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journal_title:Cell adhesion & migration
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abstract::Renal cell carcinoma (RCC) is the third most frequent malignancy within urological oncology. However, the mechanisms responsible for RCC metastasis are still needed further illustration. Our present study revealed that a seven-transmembrane receptor G-protein coupled estrogen receptor (GPER) was highly detected in var...
journal_title:Cell adhesion & migration
pub_type: 杂志文章
doi:10.4161/19336918.2014.990781
更新日期:2018-03-04 00:00:00
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journal_title:Cell adhesion & migration
pub_type: 杂志文章,评审
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journal_title:Cell adhesion & migration
pub_type: 杂志文章,评审
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journal_title:Cell adhesion & migration
pub_type: 杂志文章
doi:10.1080/19336918.2016.1227910
更新日期:2017-07-04 00:00:00
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journal_title:Cell adhesion & migration
pub_type: 评论,杂志文章
doi:10.4161/cam.25651
更新日期:2013-07-01 00:00:00
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journal_title:Cell adhesion & migration
pub_type: 杂志文章
doi:10.4161/19336918.2014.970004
更新日期:2014-01-01 00:00:00
abstract::In addition to its well-defined role as an antagonist in apoptosis, we propose that BCL2 may act as an intracellular suppressor of cell motility and adhesion under certain conditions. Our evidence shows that, when over-expressed in both cancer and non-cancer cells, BCL2 can form a complex with actin and gelsolin that ...
journal_title:Cell adhesion & migration
pub_type: 杂志文章
doi:10.4161/cam.5.1.13175
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journal_title:Cell adhesion & migration
pub_type: 杂志文章
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journal_title:Cell adhesion & migration
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journal_title:Cell adhesion & migration
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journal_title:Cell adhesion & migration
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journal_title:Cell adhesion & migration
pub_type: 杂志文章
doi:10.4161/cam.1.4.5448
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abstract::PEAR1 is highly expressed at bovine MDSC differentiation. However, its biological function remains unclear. Western blotting results showed that PEAR1 increased between day 0 and day 2 of cell differentiation and decreased from day 3. Moreover, scratch test showed that wound healing rate increased after PEAR1 overexpr...
journal_title:Cell adhesion & migration
pub_type: 杂志文章
doi:10.1080/19336918.2019.1619434
更新日期:2019-12-01 00:00:00
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journal_title:Cell adhesion & migration
pub_type: 杂志文章,评审
doi:10.4161/cam.1.3.4662
更新日期:2007-07-01 00:00:00
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journal_title:Cell adhesion & migration
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abstract::Since the time it was pioneered in 1992, intravital imaging of tumors at cellular resolution has offered us the extremely important opportunity of "seeing biology." However, until now, most studies were monitoring tumor cell behavior in the same animal over short times, requiring the combining of acquired data into a ...
journal_title:Cell adhesion & migration
pub_type: 杂志文章
doi:10.4161/cam.3.4.9581
更新日期:2009-10-01 00:00:00
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journal_title:Cell adhesion & migration
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journal_title:Cell adhesion & migration
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更新日期:2013-11-01 00:00:00