Abstract:
:Adhesion of epithelial cell to each other and to extracellular matrix, as well as cell migration ability and cytoskeleton organization undergo significant alterations in the course of neoplastic transformation, but regulatory mechanisms involved in these processes are not fully understood. Here, we studied the role of a Rho GAP protein GRAF1 (GTPase Regulator Associated with Focal adhesion kinase-1) in the regulation of the epithelial phenotype in cells of breast derived, non-malignant, MCF10A cell line. GRAF1 was shown to be localized to cell-cell junctions, and its depletion resulted in accelerated cell migration velocity, elongation of the cells and cell colonies, impaired monolayer integrity and significant disruption of desmosomes with a loss of associated keratin filaments. These processes were accompanied by formation of larger focal adhesions, an increased number of contractile actin stress fibers, reduction in epithelial markers and increase in mesenchymal markers such as epithelial-mesenchymal transition (EMT)-specific transcription factors Snail-1 and Snail-2, as well as N-cadherin, and vimentin. Moreover, unlike control cells, GRAF1 knocked-down cells demonstrated anchorage-independent growth in soft agar. GRAF1 expression in several highly invasive breast cancer cell lines was low, as compared to the non-malignant MCF10A cells, while overexpressing of GRAF1 in the malignant BT-549 cell line led to a decrease of mesenchymal markers, especially the Snail-1 and 2. Altogether, our analysis suggests that GRAF1 plays a role in the maintenance of normal epithelial phenotype and its depletion leads to an EMT-like process that might be involved in neoplastic transformation.
journal_name
Cell Adh Migrjournal_title
Cell adhesion & migrationauthors
Regev M,Sabanay H,Kartvelishvily E,Kam Z,Bershadsky ADdoi
10.1080/19336918.2016.1227910subject
Has Abstractpub_date
2017-07-04 00:00:00pages
367-383issue
4eissn
1933-6918issn
1933-6926journal_volume
11pub_type
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journal_title:Cell adhesion & migration
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journal_title:Cell adhesion & migration
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journal_title:Cell adhesion & migration
pub_type: 评论,杂志文章
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journal_title:Cell adhesion & migration
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journal_title:Cell adhesion & migration
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journal_title:Cell adhesion & migration
pub_type: 杂志文章
doi:10.4161/cam.4.3.11131
更新日期:2010-07-01 00:00:00
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journal_title:Cell adhesion & migration
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journal_title:Cell adhesion & migration
pub_type: 杂志文章
doi:10.1080/19336918.2016.1183867
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journal_title:Cell adhesion & migration
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journal_title:Cell adhesion & migration
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journal_title:Cell adhesion & migration
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