A Drosophila Model for Screening Antiobesity Agents.

Abstract:

:Although triacylglycerol, the major component for lipid storage, is essential for normal physiology, its excessive accumulation causes obesity in adipose tissue and is associated with organ dysfunction in nonadipose tissue. Here, we focused on the Drosophila model to develop therapeutics for preventing obesity. The brummer (bmm) gene in Drosophila melanogaster is known to be homologous with human adipocyte triglyceride lipase, which is related to the regulation of lipid storage. We established a Drosophila model for monitoring bmm expression by introducing the green fluorescent protein (GFP) gene as a downstream reporter of the bmm promoter. The third-instar larvae of Drosophila showed the GFP signal in all tissues observed and specifically in the salivary gland nucleus. To confirm the relationship between bmm expression and obesity, the effect of oral administration of glucose diets on bmm promoter activity was analyzed. The Drosophila flies given high-glucose diets showed higher lipid contents, indicating the obesity phenotype; this was suggested by a weaker intensity of the GFP signal as well as reduced bmm mRNA expression. These results demonstrated that the transgenic Drosophila model established in this study is useful for screening antiobesity agents. We also report the effects of oral administration of histone deacetylase inhibitors and some vegetables on the bmm promoter activity.

journal_name

Biomed Res Int

authors

Men TT,Thanh DN,Yamaguchi M,Suzuki T,Hattori G,Arii M,Huy NT,Kamei K

doi

10.1155/2016/6293163

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

6293163

eissn

2314-6133

issn

2314-6141

journal_volume

2016

pub_type

杂志文章
  • Prevalence and correlates of binge drinking among young adults using alcohol: a cross-sectional survey.

    abstract:BACKGROUND:Although binge drinking prevalence and correlates among young people have been extensively studied in the USA and Northern Europe, less is known for Southern Europe countries with relatively healthier drinking cultures. OBJECTIVE:We aimed at analyzing prevalence and correlates of binge drinking in a represe...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2014/930795

    authors: Bartoli F,Carretta D,Crocamo C,Schivalocchi A,Brambilla G,Clerici M,Carrà G

    更新日期:2014-01-01 00:00:00

  • Pulmonary Rehabilitation for Exercise Tolerance and Quality of Life in IPF Patients: A Systematic Review and Meta-Analysis.

    abstract:Objective:The aim of this study is to evaluate the efficacy and safety of pulmonary rehabilitation (PR) in patients with idiopathic pulmonary fibrosis (IPF). Methods. Embase, PubMed, Cochrane Library, China National Knowledge Infrastructure (CNKI), Chongqing VIP (CQVIP), Wanfang Data, and Chinese Biomedical Literature ...

    journal_title:BioMed research international

    pub_type: 杂志文章,meta分析

    doi:10.1155/2019/8498603

    authors: Yu X,Li X,Wang L,Liu R,Xie Y,Li S,Li J

    更新日期:2019-03-21 00:00:00

  • Awareness of Biomedical Waste Management in Dental Students in Different Dental Colleges in Nepal.

    abstract:Aim:The aim of this study is to assess the awareness of biomedical waste management in dental students of various dental colleges of Nepal. Methodology:A structured pretested questionnaire was used among 434 (323 males and 111 females) undergraduate dental students of five different dental colleges of Nepal. First par...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2018/1742326

    authors: Singh T,Ghimire TR,Agrawal SK

    更新日期:2018-12-09 00:00:00

  • The Effects of Titanium Surfaces Modified with an Antimicrobial Peptide GL13K by Silanization on Polarization, Anti-Inflammatory, and Proinflammatory Properties of Macrophages.

    abstract::The polarization of macrophages and its anti-inflammatory and proinflammatory properties play a significant role in host response after implant placement to determine the outcome of osseointegration and long-term survival. In the previous study, we immobilized an antimicrobial peptide, GL13K, onto titanium surfaces to...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2020/2327034

    authors: Chen X,Zhou L,Wu D,Huang W,Lin Y,Zhou B,Chen J

    更新日期:2020-07-24 00:00:00

  • The Use of Ropivacaine in Therapeutic Treatment of Oral Aphthosis.

    abstract::The use of anaesthetic drugs in the treatment of oral aphthosis is one of the pharmaceutical possibilities that a doctor can use for the most painful forms. Normally, Lidocaine or Diclofenac is used to treat this disease, but they can be used for a very limited time and so they are of little practical use. In this stu...

    journal_title:BioMed research international

    pub_type: 临床试验,杂志文章

    doi:10.1155/2018/1868254

    authors: Gasparini G,Saponaro G,Gasparini D,Foresta E,Azzuni C,Adduci A,Boniello R,Moro A,De Angelis P,Di Nardo F,Damato G,Doneddu P,Todaro M,Garagiola U,Pelo S

    更新日期:2018-03-11 00:00:00

  • Effect of supracervical apposition and spontaneous labour on apoptosis and matrix metalloproteinases in human fetal membranes.

    abstract:BACKGROUND:Apoptosis and matrix metalloproteinase (MMP-9) are capable of hydrolysing components of the extracellular matrix and weakening the fetal membranes which leads to eventual rupture, a key process of human parturition. The aim of this study was to determine the effect of supracervical apposition and spontaneous...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2013/316146

    authors: Chai M,Walker SP,Riley C,Rice GE,Permezel M,Lappas M

    更新日期:2013-01-01 00:00:00

  • The Diagnostic Accuracy of Ultrasound in Assessment of Myometrial Invasion in Endometrial Cancer: Subjective Assessment versus Objective Techniques.

    abstract::The aim of this study was to assess the diagnostic accuracy of subjective ultrasound evaluation of myometrial invasion of endometrial cancer and to compare its accuracy to objective methods. All consecutive patients with histologically proven endometrial cancer, who underwent ultrasound evaluation followed by surgical...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2017/1318203

    authors: Frühauf F,Zikan M,Semeradova I,Dundr P,Nemejcova K,Dusek L,Cibula D,Fischerova D

    更新日期:2017-01-01 00:00:00

  • Diagnosis and Treatment of Human Salmonellosis in Addis Ababa City, Ethiopia.

    abstract:Background:Diagnosis using reliable tools and treatment following in vitro antimicrobial susceptibility tests are critical to proper addressing of antibiotic-resistant Salmonella infection. Methodology:A cross-sectional study was conducted to assess the practice of diagnosis and treatment of salmonellosis in Addis Aba...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2018/6406405

    authors: Garedew L,Solomon S,Worku Y,Worku H,Gemeda D,Lelissa G,Mamuye Y,Abubeker R,Mihret A,Fentaw S,Worku A,Bahiru M,Erenso G

    更新日期:2018-05-23 00:00:00

  • Murine aortic smooth muscle cells acquire, though fail to present exogenous protein antigens on major histocompatibility complex class II molecules.

    abstract::In the present study aortic murine smooth muscle cell (SMC) antigen presentation capacity was evaluated using the Eα-GFP/Y-Ae system to visualize antigen uptake through a GFP tag and tracking of Eα peptide/MHCII presentation using the Y-Ae Ab. Stimulation with IFN-γ (100 ng/mL) for 72 h caused a significant (P < 0.01)...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2014/949845

    authors: Maddaluno M,MacRitchie N,Grassia G,Ialenti A,Butcher JP,Garside P,Brewer JM,Maffia P

    更新日期:2014-01-01 00:00:00

  • The Additional Effect of Autologous Platelet Concentrates to Coronally Advanced Flap in the Treatment of Gingival Recessions: A Systematic Review and Meta-Analysis.

    abstract:Background:To improve the efficacy of regenerative treatment for gingival recessions, the autologous platelet concentrates (APCs) combined with coronally advanced flap (CAF) have been investigated. However, few studies systematically assess the complementary effect of APCs in periodontal regeneration. The present study...

    journal_title:BioMed research international

    pub_type: 杂志文章,meta分析

    doi:10.1155/2019/2587245

    authors: Li R,Liu Y,Xu T,Zhao H,Hou J,Wu Y,Zhang D

    更新日期:2019-07-25 00:00:00

  • Pharmacokinetics and Bioavailability Study of Monocrotaline in Mouse Blood by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry.

    abstract:Background and Aims:The present study aimed to develop a simple and sensitive method for quantitative determination of monocrotaline (MCT) in mouse blood employing ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI/MS/MS) using rhynchophylline as an internal standard. Me...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2018/1578643

    authors: Chen L,Zhang B,Liu J,Fan Z,Weng Z,Geng P,Wang X,Lin G

    更新日期:2018-08-13 00:00:00

  • Changing Expression Profiles and Inclination to Competing Endogenous RNA Networks on MAPK Signaling Pathways of Human Adipose-Derived Stem Cells in a Direct Current Electric Field.

    abstract::Adipose-derived stem cells (ADSCs) are an abundant cell source and provide an easy way to harvest mesenchymal stem cells, which are the focus of considerable attention in regenerative medicine. Electric fields (EF) play roles in many biological events and have been reported to promote cell proliferation, migration, an...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2020/7134719

    authors: Jin M,Zhang Y,Bian Y,Qi R,Gao X

    更新日期:2020-11-06 00:00:00

  • Serum CCL21 as a Potential Biomarker for Cognitive Impairment in Spinal Cord Injury.

    abstract:Objective:Cognitive impairment is considered to be an important complication of spinal cord injury (SCI), but its underlying mechanism remains unclear. The purpose of this study is to explore whether serum CCL21 can be used as a potential biomarker of cognitive impairment in SCI. Methods:In Neck-Shoulder and Lumbocrur...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2020/6692802

    authors: Chen Y,Liang L,Cao S,Hou G,Zhang Q,Ma H,Shi B

    更新日期:2020-12-09 00:00:00

  • Pathophysiology of spasticity: implications for neurorehabilitation.

    abstract::Spasticity is the velocity-dependent increase in muscle tone due to the exaggeration of stretch reflex. It is only one of the several components of the upper motor neuron syndrome (UMNS). The central lesion causing the UMNS disrupts the balance of supraspinal inhibitory and excitatory inputs directed to the spinal cor...

    journal_title:BioMed research international

    pub_type: 杂志文章,评审

    doi:10.1155/2014/354906

    authors: Trompetto C,Marinelli L,Mori L,Pelosin E,Currà A,Molfetta L,Abbruzzese G

    更新日期:2014-01-01 00:00:00

  • Coronary Computed Tomography Angiography in Combination with Coronary Artery Calcium Scoring for the Preoperative Cardiac Evaluation of Liver Transplant Recipients.

    abstract::Liver transplantation is the best treatment option for early-stage hepatocellular carcinoma, liver cirrhosis, fulminant liver failure, and end-stage liver diseases. Even though advances in surgical techniques and perioperative care have improved postoperative outcomes, perioperative cardiovascular complications are a ...

    journal_title:BioMed research international

    pub_type: 杂志文章,评审

    doi:10.1155/2017/4081525

    authors: Choi JM,Kong YG,Kang JW,Kim YK

    更新日期:2017-01-01 00:00:00

  • Construction Formula of Biological Age Using the Principal Component Analysis.

    abstract::The biological age (BA) equation is a prediction model that utilizes an algorithm to combine various biological markers of ageing. Different from traditional concepts, the BA equation does not emphasize the importance of a golden index but focuses on using indices of vital organs to represent the senescence of whole b...

    journal_title:BioMed research international

    pub_type: 杂志文章,评审

    doi:10.1155/2016/4697017

    authors: Jia L,Zhang W,Jia R,Zhang H,Chen X

    更新日期:2016-01-01 00:00:00

  • Expression of Adiponectin Receptors on Peripheral Blood Leukocytes of Hypertensive Children Is Associated with the Severity of Hypertension.

    abstract::The aim of the study was to find out whether peripheral blood leukocyte adiponectin receptors 1 and 2 (AdipoR1, AdipoR2) protein expression patterns (flow cytometry) differ between the primary hypertension children (n = 57) and healthy controls (n = 19) and if their expression levels are related to selected clinical p...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2015/742646

    authors: Gackowska L,Litwin M,Trojanek J,Eljaszewicz A,Kubiszewska I,Niemirska A,Wierzbicka A,Michalkiewicz J

    更新日期:2015-01-01 00:00:00

  • Impact of Moderate Heat, Carvacrol, and Thymol Treatments on the Viability, Injury, and Stress Response of Listeria monocytogenes.

    abstract::The microbial safety and stability of minimally processed foods are based on the application of combined preservative factors. Since microorganisms are able to develop adaptive networks to survive under conditions of stress, food safety may be affected, and therefore understanding of stress adaptive mechanisms plays a...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2015/548930

    authors: Guevara L,Antolinos V,Palop A,Periago PM

    更新日期:2015-01-01 00:00:00

  • The Construct and Measurement of Perceived Risk of Nonremunerated Blood Donation: Evidence from the Chinese Public.

    abstract::The perceived risk of nonremunerated blood donation (NRBD) is one of the most important factors which hinder the Chinese public from donating blood. To understand deeply and measure scientifically the public's perceived risk of NRBD, in this paper the qualitative and quantitative methods were used to explore the const...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2015/302043

    authors: Chen L,Ma Z

    更新日期:2015-01-01 00:00:00

  • Norvaline Reduces Blood Pressure and Induces Diuresis in Rats with Inherited Stress-Induced Arterial Hypertension.

    abstract::Growing evidence suggests that increased arginase activity affects vital bioprocesses in various systems and universally mediates the pathogenesis of numerous metabolic diseases. The adverse effects of arginase are associated with a severe decline in L-arginine bioavailability, which leads to nitric oxide synthase sub...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2020/4935386

    authors: Gilinsky MA,Polityko YK,Markel AL,Latysheva TV,Samson AO,Polis B,Naumenko SE

    更新日期:2020-02-12 00:00:00

  • Expression and complex formation of MMP9, MMP2, NGAL, and TIMP1 in porcine myocardium but not in skeletal muscles in male pigs with tachycardia-induced systolic heart failure.

    abstract::Matrix metalloproteinases (MMPs) are involved in the remodeling of extracellular matrix in various tissues. Their functioning could be related to the formation of complexes, containing MMP9, MMP2, tissue inhibitor of metalloproteinases type 1 (TIMP1), and neutrophil gelatinase-associated lipocalin (NGAL). Such complex...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2013/283856

    authors: Kiczak L,Tomaszek A,Bania J,Paslawska U,Zacharski M,Noszczyk-Nowak A,Janiszewski A,Skrzypczak P,Ardehali H,Jankowska EA,Ponikowski P

    更新日期:2013-01-01 00:00:00

  • Enhanced blood compatibility of metallocene polyethylene subjected to hydrochloric acid treatment for cardiovascular implants.

    abstract::Blood compatibility of metallocene polyethylene (mPE) was investigated after modifying the surface using hydrochloric acid. Contact angle of the mPE exposed to HCl poses a decrease in its value which indicates increasing wettability and better blood compatibility. Surface of mPE analyzed by using FTIR revealed no sign...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2014/963149

    authors: Jaganathan SK,Mohandas H,Sivakumar G,Kasi P,Sudheer T,Avineri Veetil S,Murugesan S,Supriyanto E

    更新日期:2014-01-01 00:00:00

  • In Vivo Imaging of Neutrophil Extracellular Traps (NETs): Visualization Methods and Outcomes.

    abstract::Neutrophils comprise the first line of innate immune defense during a host-pathogen interaction. They attack microorganisms directly through three different methods, of which, phagocytosis and degranulation have been known and well-studied for decades. The formation of neutrophil extracellular traps (NETs) is the thir...

    journal_title:BioMed research international

    pub_type: 杂志文章,评审

    doi:10.1155/2020/4192745

    authors: Alasmari SZ

    更新日期:2020-02-01 00:00:00

  • Levels of Soluble CD30 and CD26 and Their Clinical Significance in Patients with Primary Immune Thrombocytopenia.

    abstract:Background:sCD30 and sCD26 are correlated with autoimmune diseases. However, little research has been done on the relationship between them and primary immune thrombocytopenia (ITP). Methods:This study enrolled 47 patients diagnosed with ITP in the Institute of Hematology and Blood Disease Hospital, Chinese Academy of...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2020/1279371

    authors: Wang H,Gu X,Li H,Yin L,Tao W,Yu J,Zhou Q,Mu H,Shen Y,Yao J,Liu L,Bi H,Yang R,Zhou Z

    更新日期:2020-03-31 00:00:00

  • Kölliker's organ and the development of spontaneous activity in the auditory system: implications for hearing dysfunction.

    abstract::Prior to the "onset of hearing," developing cochlear inner hair cells (IHCs) and primary auditory neurons undergo experience-independent activity, which is thought to be important in retaining and refining neural connections in the absence of sound. One of the major hypotheses regarding the origin of such activity inv...

    journal_title:BioMed research international

    pub_type: 杂志文章,评审

    doi:10.1155/2014/367939

    authors: Dayaratne MW,Vlajkovic SM,Lipski J,Thorne PR

    更新日期:2014-01-01 00:00:00

  • Chronic Health Conditions as a Risk Factor for Falls among the Community-Dwelling US Older Adults: A Zero-Inflated Regression Modeling Approach.

    abstract::Falls are an important health concern among older adults due to age-related changes in the body. Having a medical history of chronic health condition may pose even higher risk of falling. Only few studies have assessed a number of chronic health conditions as risk factor for falls over a large nationally representativ...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2017/5146378

    authors: Paliwal Y,Slattum PW,Ratliff SM

    更新日期:2017-01-01 00:00:00

  • Screening and Molecular Identification of Pectinase Producing Microbes from Coffee Pulp.

    abstract::Application of enzymes in biotechnological process has expanded considerably in recent years. In food and related industry, major importance was being attached to the use of enzymes in upgrading quality, increasing yields of extractive processes, product stabilization, and improvement of flavor and byproduct utilizati...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2018/2961767

    authors: Oumer OJ,Abate D

    更新日期:2018-04-03 00:00:00

  • Anion Gap Was Associated with Inhospital Mortality and Adverse Clinical Outcomes of Coronary Care Unit Patients.

    abstract:Background:Anion gap (AG) has been proved to be associated with prognosis of many cardiovascular diseases. This study is aimed at exploring the association of AG with inhospital all-cause mortality and adverse clinical outcomes in coronary care unit (CCU) patients. Method:All data of this study was extracted from Medi...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2020/4598462

    authors: Sun T,Cai C,Shen H,Yang J,Guo Q,Zhang J,Zhang B,Ding Y,Zhou Y

    更新日期:2020-08-27 00:00:00

  • Laser Scanning In Vivo Confocal Microscopy of Clear Grafts after Penetrating Keratoplasty.

    abstract:PURPOSE:To evaluate the changes of keratocytes and dendritic cells in the central clear graft by laser scanning in vivo confocal microscopy after penetrating keratoplasty (PK). METHODS:Thirty adult subjects receiving PK at Shandong Eye Institute and with clear grafts and no sign of immune rejection after surgery were ...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2016/5159746

    authors: Wang D,Song P,Wang S,Sun D,Wang Y,Zhang Y,Gao H

    更新日期:2016-01-01 00:00:00

  • A phylogenetic analysis of Greek isolates of Aspergillus species based on morphology and nuclear and mitochondrial gene sequences.

    abstract::Aspergillus species originating from Greece were examined by morphological and molecular criteria to explore the diversity of this genus. The phylogenetic relationships of these species were determined using sequences from the ITS and IGS region of the nuclear rRNA gene complex, two nuclear genes ( β -tubulin (benA) a...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2013/260395

    authors: Krimitzas A,Pyrri I,Kouvelis VN,Kapsanaki-Gotsi E,Typas MA

    更新日期:2013-01-01 00:00:00