Abstract:
:In the present study aortic murine smooth muscle cell (SMC) antigen presentation capacity was evaluated using the Eα-GFP/Y-Ae system to visualize antigen uptake through a GFP tag and tracking of Eα peptide/MHCII presentation using the Y-Ae Ab. Stimulation with IFN-γ (100 ng/mL) for 72 h caused a significant (P < 0.01) increase in the percentage of MHC class II positive SMCs, compared with unstimulated cells. Treatment with Eα-GFP (100 μg/mL) for 48 h induced a significant (P < 0.05) increase in the percentage of GFP positive SMCs while it did not affect the percentage of Y-Ae positive cells, being indicative of antigen uptake without its presentation in the context of MHC class II. After IFN-γ-stimulation, ovalbumin- (OVA, 1 mg/mL) or OVA323-339 peptide-(0.5 μg/mL) treated SMCs failed to induce OT-II CD4(+) T cell activation/proliferation; this was also accompanied by a lack of expression of key costimulatory molecules (OX40L, CD40, CD70, and CD86) on SMCs. Finally, OVA-treated SMCs failed to induce DO11.10-GFP hybridoma activation, a process independent of costimulation. Our results demonstrate that while murine primary aortic SMCs express MHC class II and can acquire exogenous antigens, they fail to activate T cells through a failure in antigen presentation and a lack of costimulatory molecule expression.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
Maddaluno M,MacRitchie N,Grassia G,Ialenti A,Butcher JP,Garside P,Brewer JM,Maffia Pdoi
10.1155/2014/949845subject
Has Abstractpub_date
2014-01-01 00:00:00pages
949845eissn
2314-6133issn
2314-6141journal_volume
2014pub_type
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journal_title:BioMed research international
pub_type: 杂志文章
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更新日期:2018-05-20 00:00:00
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journal_title:BioMed research international
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doi:10.1155/2014/583035
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doi:10.1155/2015/413076
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journal_title:BioMed research international
pub_type: 杂志文章,随机对照试验
doi:10.1155/2016/8357621
更新日期:2016-01-01 00:00:00
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pub_type: 临床试验,杂志文章
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更新日期:2017-01-01 00:00:00
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journal_title:BioMed research international
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更新日期:2013-01-01 00:00:00
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journal_title:BioMed research international
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journal_title:BioMed research international
pub_type: 杂志文章
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更新日期:2015-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
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journal_title:BioMed research international
pub_type: 杂志文章
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更新日期:2020-02-27 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/405860
更新日期:2014-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
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更新日期:2015-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/2647879
更新日期:2017-01-01 00:00:00