Abstract:
:Vibrio cholerae is the bacterial causative agent of the human disease cholera. Non-pathogenic bacterium can be converted to pathogenic following infection by a filamentous phage, CTXΦ, that carries the cholera toxin encoding genes. A crucial step during phage infection requires a direct interaction between the CTXΦ minor coat protein (pIII(CTX)) and the C-terminal domain of V. cholerae TolA protein (TolAIIIvc). In order to get a better understanding of TolA function during the infection process, we have initiated a study of the V. cholerae TolAIII domain by 2D and 3D heteronuclear NMR. With the exception of the His-tag (H123-H128), 97 % of backbone (1)H, (15)N and (13)C resonances were assigned and the side chain assignments for 92 % of the protein were obtained (BMRB deposit with accession number 25689).
journal_name
Biomol NMR Assignjournal_title
Biomolecular NMR assignmentsauthors
Navarro R,Bornet O,Houot L,Lloubes R,Guerlesquin F,Nouailler Mdoi
10.1007/s12104-016-9690-ysubject
Has Abstractpub_date
2016-10-01 00:00:00pages
311-3issue
2eissn
1874-2718issn
1874-270Xpii
10.1007/s12104-016-9690-yjournal_volume
10pub_type
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