Abstract:
:The bacterium Geobacter metallireducens is capable of transferring electrons to the cell exterior, a process designated extracellular electron transfer. This mechanism allows the microorganism to reduce extracellular acceptors such as Fe(III) (hydr)oxides and water toxic and/or radioactive contaminants including Cr(VI) and U(VI). It is also capable of oxidizing waste water aromatic organic compounds being an important microorganism for bioremediation of polluted waters. Extracellular electron transfer also allows electricity harvesting from microbial fuel cells, a promising sustainable form of energy production. However, extracellular electron transfer processes in this microorganism are still poorly characterized. The triheme c-type cytochrome PpcA from G. metallireducens is abundant in the periplasm and is crucial for electron transfer between the cytoplasm and the cell's exterior. In this work, we report near complete assignment of backbone, side chain and heme resonances for PpcA in the oxidized state that will permit its structure determination and identification of interactions with physiological redox partners.
journal_name
Biomol NMR Assignjournal_title
Biomolecular NMR assignmentsauthors
Portela PC,Dantas JM,Salgueiro CAdoi
10.1007/s12104-019-09916-8subject
Has Abstractpub_date
2020-04-01 00:00:00pages
31-36issue
1eissn
1874-2718issn
1874-270Xpii
10.1007/s12104-019-09916-8journal_volume
14pub_type
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journal_title:Biomolecular NMR assignments
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journal_title:Biomolecular NMR assignments
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journal_title:Biomolecular NMR assignments
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journal_title:Biomolecular NMR assignments
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journal_title:Biomolecular NMR assignments
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journal_title:Biomolecular NMR assignments
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journal_title:Biomolecular NMR assignments
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journal_title:Biomolecular NMR assignments
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journal_title:Biomolecular NMR assignments
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journal_title:Biomolecular NMR assignments
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journal_title:Biomolecular NMR assignments
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journal_title:Biomolecular NMR assignments
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journal_title:Biomolecular NMR assignments
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journal_title:Biomolecular NMR assignments
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journal_title:Biomolecular NMR assignments
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journal_title:Biomolecular NMR assignments
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journal_title:Biomolecular NMR assignments
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journal_title:Biomolecular NMR assignments
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journal_title:Biomolecular NMR assignments
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