Abstract:
:Tuberculosis is one of the deadliest diseases worldwide affecting approximately 10 million people in 2018. This classifies tuberculosis as epidemic in several countries and leads to an increasing number of multidrug-resistant strains. Thus, the development of new drugs is essential to effective treatments. A potential drug target is the ribose-5-phosphate isomerase, a ubiquitous enzyme important to nucleotide and cofactor biosynthesis. Here, we report the backbone assignment of ribose-5-phosphate isomerase of Mycobacterium tuberculosis (MtRpiB) that has been performed by triple resonance sequential approach using a [13C, 15N, 2H]-labeled protein. This is the first ribose-5-phosphate isomerase, an enzyme previously classified as highly druggable, to be assigned. These data will be important to further screening studies to find inhibitors and determine their interaction with MtRpiB.
journal_name
Biomol NMR Assignjournal_title
Biomolecular NMR assignmentsauthors
Bartkevihi L,Martins B,de Oliveira DMP,Pires JRM,Anobom CD,Almeida FCLdoi
10.1007/s12104-020-09931-0subject
Has Abstractpub_date
2020-04-01 00:00:00pages
119-122issue
1eissn
1874-2718issn
1874-270Xpii
10.1007/s12104-020-09931-0journal_volume
14pub_type
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