当前位置： SCI文献检索 > Biomolecular NMR Assignments期刊下所有文献 > 1H, 15N, 13C backbone resonance assignment of the C-terminal domain of enzyme I from Thermoanaerobacter tengcongensis.

1H, 15N, 13C backbone resonance assignment of the C-terminal domain of enzyme I from Thermoanaerobacter tengcongensis.

Abstract：

:Phosphoenolpyruvate binding to the C-terminal domain (EIC) of enzyme I of the bacterial phosphotransferase system (PTS) initiates a phosphorylation cascade that results in sugar translocation across the cell membrane and controls a large number of essential pathways in bacterial metabolism. EIC undergoes an expanded to compact conformational equilibrium that is regulated by ligand binding and determines the phosphorylation state of the overall PTS. Here, we report the backbone 1H, 15N and 13C chemical shift assignments of the 70 kDa EIC dimer from the thermophilic bacterium Thermoanaerobacter tengcongensis. Assignments were obtained at 70 °C by heteronuclear multidimensional NMR spectroscopy. In total, 90% of all backbone resonances were assigned, with 264 out of a possible 299 residues assigned in the 1H-15N TROSY spectrum. The secondary structure predicted from the assigned backbone resonance using the program TALOS+ is in good agreement with the X-ray crystal structure of T. tengcongensis EIC. The reported assignments will allow detailed structural and thermodynamic investigations on the coupling between ligand binding and conformational dynamics in EIC.

journal_name

Biomol NMR Assign

journal_title

Biomolecular NMR assignments

authors

Dotas RR,Venditti V

doi

10.1007/s12104-017-9788-x

subject

Has Abstract

pub_date

2018-04-01 00:00:00

pages

103-106

issue

1

eissn

1874-2718

issn

1874-270X

pii

10.1007/s12104-017-9788-x

journal_volume

12

pub_type

杂志文章

相关文献

Biomolecular NMR Assignments文献大全
  • Backbone and side-chain 1H, 13C and 15N assignments of the ubiquitin-associated domain of human X-linked inhibitor of apoptosis protein.

    abstract：:X-linked inhibitor of apoptosis protein (XIAP), a leading member of the family of inhibitor of apoptosis (IAP) proteins, is considered as the most potent and versatile inhibitor of caspases and apoptosis. It has been reported that XIAP is frequently overexpressed in cancer and its expression level is implicated in con...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-009-9197-x

    authors： Hui SK,Tse MK,Yang Y,Wong BC,Sze KH

    更新日期：2010-04-01 00:00:00

  • NMR resonance assignment of the autoimmunity protein SpaI from Bacillus subtilis ATCC 6633.

    abstract：:Bacillus subtilis ATCC 6633 produces the lipid II targeting lantibiotic subtilin. For self-protection these gram-positive bacteria express a cluster of four self-immunity proteins named SpaIFEG. SpaI is a 16.8 kDa lipoprotein which is attached to the outside of the cytoplasmic membrane via a covalently linked diacylgl...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-011-9314-5

    authors： Christ NA,Duchardt-Ferner E,Düsterhus S,Kötter P,Entian KD,Wöhnert J

    更新日期：2012-04-01 00:00:00

  • 1H, 13C, and 15N backbone, side-chain, and heme chemical shift assignments for oxidized and reduced forms of the monoheme c-type cytochrome ApcA isolated from the acidophilic metal-reducing bacterium Acidiphilium cryptum.

    abstract：:We report the (1)H, (13)C, and (15)N chemical shift assignments of both oxidized and reduced forms of an abundant periplasmic c-type cytochrome, designated ApcA, isolated from the acidophilic gram-negative facultatively anaerobic metal-reducing alphaproteobacterium Acidiphilium cryptum. These resonance assignments pro...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-010-9274-1

    authors： Cort JR,Swenson MW,Magnuson TS

    更新日期：2011-04-01 00:00:00

  • Resonance assignments of wild-type and two cysteine-free variants of the four-helix bundle protein, Rop.

    abstract：:Repressor of primer (Rop, or ROM, RNA I modulator) is a 63 amino acid four-helix bundle protein that exists in solution as an anti-parallel homodimer. This protein has been extensively studied, including by X-ray crystallography, NMR, rational design, and combinatorial mutagenesis. Previous NMR experiments with wild-t...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-018-9837-0

    authors： Bowles DP,Yuan C,Stephany KR,Lavinder JJ,Hansen AL,Magliery TJ

    更新日期：2018-10-01 00:00:00

  • ¹H, ¹³C and ¹⁵N resonance assignments of Ca²⁺-bound human S100A15.

    abstract：:S100A15 (koebnerisin) is overexpressed in psoriatic skin and displays distinct localizations in skin and breast with divergent functions in inflammation. Here we report the backbone and side-chain resonance assignments for the Ca(2+)-bound human S100A15. ...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-013-9476-4

    authors： Cho CC,Hung KW,Yu C

    更新日期：2014-04-01 00:00:00

  • Backbone resonance assignments of complexes of apo human calmodulin bound to IQ motif peptides of voltage-dependent sodium channels NaV1.1, NaV1.4 and NaV1.7.

    abstract：:Human voltage-gated sodium (NaV) channels are critical for initiating and propagating action potentials in excitable cells. Nine isoforms have different roles but similar topologies, with a pore-forming α-subunit and auxiliary transmembrane β-subunits. NaV pathologies lead to debilitating conditions including epilepsy...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-018-9824-5

    authors： Isbell HM,Kilpatrick AM,Lin Z,Mahling R,Shea MA

    更新日期：2018-10-01 00:00:00

  • NMR assignments of the C-terminal domain of human galectin-8.

    abstract：:Galectins recognize β-galectosides to promote a variety of cellular functions. Despite their sequence variations, all galectins share the same carbohydrate recognition domains (CRD) and their modes of ligand recognition at a structural level are essentially identical. Human galectin 8 plays an important role in numero...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-015-9623-1

    authors： Liu CH,Chien CT,Lin CH,Hsu ST

    更新日期：2015-10-01 00:00:00

  • 1H, 13C and 15N backbone resonance assignment of the human BRCA2 N-terminal region.

    abstract：:The Breast Cancer susceptibility protein 2 (BRCA2) is involved in mechanisms that maintain genome stability, including DNA repair, replication and cell division. These functions are ensured by the folded C-terminal DNA binding domain of BRCA2 but also by its large regions predicted to be disordered. Several studies ha...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-019-09924-8

    authors： Julien M,Miron S,Carreira A,Theillet FX,Zinn-Justin S

    更新日期：2020-04-01 00:00:00

  • ¹H, ¹⁵N and ¹³C Backbone resonance assignments of a conformational mutant of the adhesion protein ∆-Bd37 from Babesia divergens.

    abstract：:We report here the resonance assignment of EDK-∆-Bd37, conformational mutant potentially displaying the "open" conformation of Bd37, a 25 kDa surface protein from the Apicomplexa parasite Babesia divergens that could undergo drastic conformational changes during erythrocyte invasion. ...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-012-9418-6

    authors： Barthe P,Murciano B,Schetters T,Gorenflot A,Delbecq S,Roumestand C

    更新日期：2013-10-01 00:00:00

  • Sequence specific 1H, 13C and 15N backbone resonance assignments of UVI31+ from Chlamydomonas reinhardtii.

    abstract：:The cDNA of UVI31+ was cloned from C. reinhardtii and expressed in E. coli from where the protein was purified to homogeneity. The purified protein exhibited beta-lactamase activity (Manuscript in preparation). However, UVI31+ has no homology with the known β-lactamases. In order to understand the structural basis of ...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-010-9239-4

    authors： Rout AK,Minda R,Peri D,Ramakrishnan V,Bhattacharjee SK,Rao BJ,Chary KV

    更新日期：2010-10-01 00:00:00

  • Backbone ¹H, ¹³C and ¹⁵N resonance assignment of the C-terminal EGF-cbEGF pair of LTBP1 and flanking residues.

    abstract：:Latent TGFβ binding protein 1 (LTBP1) is a large extracellular protein that has been shown to bind covalently to the propeptide of TGFβ cytokines and form a large latent complex, which is then incapable of binding TGFβ receptors. LTBP1 has also been demonstrated to interact with a number of insoluble extracellular mat...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-013-9474-6

    authors： Robertson IB,Handford PA,Redfield C

    更新日期：2014-04-01 00:00:00

  • 1H, 13C and 15N NMR chemical shift assignments of A. thaliana RCD1 RST.

    abstract：:The A. thaliana RCD1 (radical-induced cell death1) protein is a cellular signaling hub protein which interacts with numerous plant transcription factors from different families. It consists of three conserved domains and intervening unstructured regions, the C-terminal RST domain being responsible for the interactions...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-017-9749-4

    authors： Tossavainen H,Hellman M,Vainonen JP,Kangasjärvi J,Permi P

    更新日期：2017-10-01 00:00:00

  • 1H, 13C and 15N resonance assignments of Q38FZ4, hypothetical protein from Trypanosoma brucei.

    abstract：:Q38FZ4 (UniProt accession number), is an 85-residue hypothetical protein from Trypanosoma brucei (T. brucei). Q38FZ4, which might be specific among the trypanosomatid genomes, shares low sequence similarity with mammal proteins and also has an essential function in the growth of T. brucei. Here we report the resonance...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-020-09956-5

    authors： Wang R,Zhang J,Liao S,Tu X

    更新日期：2020-10-01 00:00:00

  • 1H, 13C and 15N resonance assignments of the arsenate reductase from Synechocystis sp. strain PCC 6803.

    abstract：:Arsenate reductases (ArsC) are a group of enzymes that play essential roles in biological arsenic detoxification pathways by catalyzing the intracellular reduction of arsenate to arsenite, which is subsequently extruded from the cells by specific transport systems. The ArsC protein from cyanobacterium Synechocystis sp...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-010-9273-2

    authors： Yu C,Xia B,Jin C

    更新日期：2011-04-01 00:00:00

  • Backbone, side chain and heme resonance assignment of the triheme cytochrome PpcA from Geobacter metallireducens in the oxidized state.

    abstract：:The bacterium Geobacter metallireducens is capable of transferring electrons to the cell exterior, a process designated extracellular electron transfer. This mechanism allows the microorganism to reduce extracellular acceptors such as Fe(III) (hydr)oxides and water toxic and/or radioactive contaminants including Cr(VI...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-019-09916-8

    authors： Portela PC,Dantas JM,Salgueiro CA

    更新日期：2020-04-01 00:00:00

  • Chemical shift assignments for F-plasmid TraI (381-569).

    abstract：:TraI, the F plasmid-encoded nickase, is a 1,756 amino acid protein essential for conjugative transfer of F plasmid DNA from one bacterium to another. While crystal structures of N- and C-terminal domains of F TraI have been determined, central domains of the protein are structurally unexplored. These middle domains (b...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-010-9269-y

    authors： Wright NT,Majumdar A,Schildbach JF

    更新日期：2011-04-01 00:00:00

  • 1H, 13C and 15N resonance assignment of domain 1 of trans-activation response element (TAR) RNA binding protein isoform 1 (TRBP2) and its comparison with that of isoform 2 (TRBP1).

    abstract：:TAR RNA binding protein (TRBP) is a double-stranded RNA binding protein involved in various biological processes like cell growth, development, death, etc. The protein exists as two isoforms TRBP2 and TRBP1. TRBP2 contains additional 21 amino acids at its N-terminus, which are proposed to be involved in its membrane l...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-018-9807-6

    authors： Paithankar H,Jadhav PV,Naglekar AS,Sharma S,Chugh J

    更新日期：2018-04-01 00:00:00

  • NMR chemical shift assignment of a constitutively active fragment of the antitermination protein LicT.

    abstract：:LicT belongs to an essential family of bacterial antitermination proteins which bind to nascent mRNAs in order to stimulate transcription of sugar-metabolizing operons. As most of other antitermination proteins involved in carbohydrate metabolism, LicT is composed of a N-terminal RNA-binding module (CAT) and two homol...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-019-09914-w

    authors： Yang Y,Declerck N,Déméné H

    更新日期：2020-04-01 00:00:00

  • 1H, 15N and 13C resonance assignments of the HR1c domain of PRK1, a protein kinase C-related kinase.

    abstract：:PRK1 is a member of the protein kinase C-related kinase (PRK) family of serine/threonine kinases and a downstream effector of Rho GTPases. PRK1 has three N-terminal Homology Region 1 (HR1) domains (HR1a, HR1b and HR1c), which form antiparallel coiled coils that interact with Rho family GTPases. PRK1 also has a C2-like...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-020-09954-7

    authors： Sophocleous G,Wood G,Owen D,Mott HR

    更新日期：2020-10-01 00:00:00

  • Backbone assignment of E. coli NfsB and the effects of addition of the cofactor analogue nicotinic acid.

    abstract：:E. coli nitroreductase NfsB (also called NfnB) has been studied extensively, largely due to its potential for cancer gene therapy. A homodimeric flavoprotein of 216 residues, it catalyses the reduction of nitroaromatics to cytotoxic hydroxylamines by NADH and NADPH and also the reduction of quinones to hydroxyquinones...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-020-09997-w

    authors： Hyde EI,Chau AK,Smith LJ

    更新日期：2021-01-09 00:00:00

  • (1)H, (15)N, and (13)C chemical shift assignments of mouse HOXA13 DNA binding domain.

    abstract：:The homeobox gene (HOXA13) codes for a transcription factor protein that binds to AT-rich DNA sequences and controls expression of many important proteins during embryonic morphogenesis. We report complete NMR chemical shift assignments of the mouse HOXA13 DNA binding domain (A13DBD; BMRB no. 16252). ...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-009-9174-4

    authors： Zhang Y,Thornburg CK,Stadler HS,Ames JB

    更新日期：2009-12-01 00:00:00

  • (1)H, (13)C and (15)N resonance assignments and secondary structure analysis of translation initiation factor 1 from Pseudomonas aeruginosa.

    abstract：:Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen and a primary cause of infection in humans. P. aeruginosa can acquire resistance against multiple groups of antimicrobial agents, including β-lactams, aminoglycosides and fluoroquinolones, and multidrug resistance is increasing in this organism which mak...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-016-9678-7

    authors： Bernal A,Hu Y,Palmer SO,Silva A,Bullard J,Zhang Y

    更新日期：2016-10-01 00:00:00

  • NMR assignments of actin depolymerizing factor (ADF) like UNC-60A and cofilin like UNC-60B proteins of Caenorhabditis elegans.

    abstract：:The actin filament dynamics in nematode, Caenorhabditis elegans, is regulated by differential activity of two proteins UNC-60A and UNC-60B. UNC-60A exhibits strong pointed end depolymerization on C. elegans actin (Ce-actin), strong inhibition of polymerization, strong monomer sequestering activity, weak severing activ...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-014-9588-5

    authors： Shukla VK,Kabra A,Yadav R,Ono S,Kumar D,Arora A

    更新日期：2015-10-01 00:00:00

  • Backbone assignment of the little finger domain of a Y-family DNA polymerase.

    abstract：:Sulfolobus solfataricus DNA polymerase IV (Dpo4), a prototype Y-family DNA polymerase, contains a unique little finger domain besides a catalytic core. Here, we report the chemical shift assignments for the backbone nitrogens, α and β carbons, and amide protons of the little finger domain of Dpo4. This work and our pu...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-011-9298-1

    authors： Ma D,Fowler JD,Suo Z

    更新日期：2011-10-01 00:00:00

  • Solid-state NMR resonance assignments of the filament-forming CARD domain of the innate immunity signaling protein MAVS.

    abstract：:The mitochondrial antiviral signalling protein (MAVS) is a central signal transduction hub in the innate immune response against viral infections. Viral RNA present in the cytoplasm is detected by retinoic acid inducible gene I like receptors, which then activate MAVS via heterotypic interactions between their respect...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-014-9579-6

    authors： He L,Lührs T,Ritter C

    更新日期：2015-10-01 00:00:00

  • NMR resonance assignments for the GSPII-B domain of the traffic ATPase PilF from Thermus thermophilus in the apo and the c-di-GMP-bound state.

    abstract：:The PilF protein from the thermophilic bacterium Thermus thermophilus is a traffic ATPase powering the assembly of the DNA translocation machinery as well as of type 4 pili. Thereby PilF mediates the natural transformability of T. thermophilus. PilF contains a C-terminal ATPase domain and three N-terminal domains with...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-019-09911-z

    authors： Neißner K,Keller H,Duchardt-Ferner E,Hacker C,Kruse K,Averhoff B,Wöhnert J

    更新日期：2019-10-01 00:00:00

  • NMR assignments of 1H, 13C and 15N resonances of the C-terminal subunit from Azotobacter vinelandii mannuronan C5-epimerase 6 (AlgE6R3).

    abstract：:The 19.9 kDa C-terminal module (R3) from Azotobacter vinelandii mannronan C5-epimerase AlgE6 has been (13)C, (15)N isotopically labelled and recombinantly expressed. We report here the (1)H, (13)C, (15)N resonance assignment of AlgE6R3. ...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-010-9259-0

    authors： Buchinger E,Skjåk-Bræk G,Valla S,Wimmer R,Aachmann FL

    更新日期：2011-04-01 00:00:00

  • Backbone NMR resonance assignment of the Abelson kinase domain in complex with imatinib.

    abstract：:Imatinib (Glivec or Gleevec) potently inhibits the tyrosine kinase activity of BCR-ABL, a constitutively activated kinase, which causes chronic myelogenous leukemia (CML). Here we report the first almost complete backbone assignment of c-ABL kinase domain in complex with imatinib. ...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-008-9079-7

    authors： Vajpai N,Strauss A,Fendrich G,Cowan-Jacob SW,Manley PW,Jahnke W,Grzesiek S

    更新日期：2008-06-01 00:00:00

  • Backbone and side-chain chemical shift assignments of p50 subunit of NF-κB transcription factor.

    abstract：:Nuclear Factor κB (NF-κB) is a family of five related transcription factors that recognize a κB DNA element on the promoter and enhancer regions of target genes and modulate their expression. Here we report a complete set of 1H, 13C, 15N backbone and side chain resonance assignments for the p50 DNA binding and dimeriz...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-020-09978-z

    authors： Singh A,Dyson HJ

    更新日期：2020-09-16 00:00:00

  • NMR assignment of the immune mapped protein 1 (IMP1) homologue from Plasmodium falciparum.

    abstract：:Plasmodium falciparum is responsible for causing cerebral malaria in humans. IMP1 is an immunogenic protein, present in the parasite, which has been shown to induce an immune response against apicomplexan parasites in a species-specific manner. Here, we report the complete NMR assignments of PfIMP1. ...

    journal_title：Biomolecular NMR assignments

    pub_type： 杂志文章

    doi：10.1007/s12104-015-9616-0

    authors： Benjamin S,Williams F,Kerry L,Matthews S

    更新日期：2015-10-01 00:00:00