NMR resonance assignment of the autoimmunity protein SpaI from Bacillus subtilis ATCC 6633.

Abstract:

:Bacillus subtilis ATCC 6633 produces the lipid II targeting lantibiotic subtilin. For self-protection these gram-positive bacteria express a cluster of four self-immunity proteins named SpaIFEG. SpaI is a 16.8 kDa lipoprotein which is attached to the outside of the cytoplasmic membrane via a covalently linked diacylglycerol anchor. Together with the ABC-transporter SpaFEG, SpaI protects the membrane from subtilin insertion and there is evidence for a direct interaction of SpaI with subtilin. As a prerequisite for further structural studies of SpaI and the SpaI/subtilin complex we report here the full (1)H, (15)N, (13)C chemical shift assignment for a stable 14.9 kDa C-terminal fragment of SpaI.

journal_name

Biomol NMR Assign

authors

Christ NA,Duchardt-Ferner E,Düsterhus S,Kötter P,Entian KD,Wöhnert J

doi

10.1007/s12104-011-9314-5

subject

Has Abstract

pub_date

2012-04-01 00:00:00

pages

9-13

issue

1

eissn

1874-2718

issn

1874-270X

journal_volume

6

pub_type

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