Abstract:
:Bacillus subtilis ATCC 6633 produces the lipid II targeting lantibiotic subtilin. For self-protection these gram-positive bacteria express a cluster of four self-immunity proteins named SpaIFEG. SpaI is a 16.8 kDa lipoprotein which is attached to the outside of the cytoplasmic membrane via a covalently linked diacylglycerol anchor. Together with the ABC-transporter SpaFEG, SpaI protects the membrane from subtilin insertion and there is evidence for a direct interaction of SpaI with subtilin. As a prerequisite for further structural studies of SpaI and the SpaI/subtilin complex we report here the full (1)H, (15)N, (13)C chemical shift assignment for a stable 14.9 kDa C-terminal fragment of SpaI.
journal_name
Biomol NMR Assignjournal_title
Biomolecular NMR assignmentsauthors
Christ NA,Duchardt-Ferner E,Düsterhus S,Kötter P,Entian KD,Wöhnert Jdoi
10.1007/s12104-011-9314-5subject
Has Abstractpub_date
2012-04-01 00:00:00pages
9-13issue
1eissn
1874-2718issn
1874-270Xjournal_volume
6pub_type
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