Abstract：
:GNA2132 (Genome-derived Neisseria Antigen 2132) is a surface-exposed lipoprotein discovered by reverse vaccinology and expressed by genetically diverse Neisseria meningitidis strains (Pizza et al. 2000). The protein induces bactericidal antibodies against most strains of Meningococccus and has been included in a multivalent recombinant vaccine against N. meningitidis serogroup B. Structure determination of GNA2132 is important for understanding the antigenic properties of the protein in view of increased efficiency vaccine development. We report practically complete (1)H, (13)C and (15)N assignment of the detectable spectrum of a highly conserved C-terminal region of GNA2132 (residues 245-427) in micellar solution, a medium used to improve the spectral quality. The first 32 residues of our construct up to residue 277 were not visible in the spectrum, presumably because of line broadening due to solvent and/or conformational exchange. Secondary structure predictions based on chemical shift information indicate the presence of an all beta-protein with eight beta strands.
journal_name
Biomol NMR Assignjournal_title
Biomolecular NMR assignmentsauthors
Esposito V,Musi V,Veggi D,Pastore A,Pizza Mdoi
10.1007/s12104-010-9220-2subject
Has Abstractpub_date
2010-04-01 00:00:00pages
107-9issue
1eissn
1874-2718issn
1874-270Xjournal_volume
4pub_type
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