Abstract：
:Light chain (AL) amyloidosis is a systemic disease characterized by the formation of immunoglobulin light-chain fibrils in critical organs of the body. The light-chain protein AL-09 presents one severe case of cardiac AL amyloidosis, which contains seven mutations in the variable domain (VL) relative to its germline counterpart, κI O18/O8 VL. Three of these mutations are non-conservative-Y87H, N34I, and K42Q-and previous work has shown that they are responsible for significantly reducing the protein's thermodynamic stability, allowing fibril formation to occur with fast kinetics and across a wide-range of pH conditions. Currently, however, there is extremely limited structural information available which explicitly describes the residues that are involved in supporting the misfolded fibril structure. Here, we assign the site-specific 15N and 13C chemical shifts of the rigid residues of AL-09 VL fibrils by solid-state NMR, reporting on the regions of the protein involved in the fibril as well as the extent of secondary structure.
journal_name
Biomol NMR Assignjournal_title
Biomolecular NMR assignmentsauthors
Piehl DW,Blancas-Mejía LM,Ramirez-Alvarado M,Rienstra CMdoi
10.1007/s12104-016-9718-3subject
Has Abstractpub_date
2017-04-01 00:00:00pages
45-50issue
1eissn
1874-2718issn
1874-270Xpii
10.1007/s12104-016-9718-3journal_volume
11pub_type
杂志文章abstract：:A pheromone-binding protein from navel orange worm, Amyelois transitella (Atra-PBP1) binds to non-polar pheromone molecules and facilitates the transport and delivery of pheromone to the membrane-bound pheromone receptors. We report complete NMR chemical shift assignments of Atra-PBP1 obtained at pH 4.5 and 25 degrees...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-008-9096-6
更新日期：2008-12-01 00:00:00
abstract：:Nervous necrosis virus (NNV) is a non-enveloped virus that causes massive mortality in aquaculture fish production worldwide. Recently X-ray crystallography and single particle cryo-EM have independently determined the icosahedral capsid of NNV to near-atomic resolutions to show the capsid protein is composed of a S-d...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-019-09921-x
更新日期：2020-04-01 00:00:00
abstract：:ITCH (aka Atrophin-1-interacting protein 4) is a prominent member of the NEDD4 HECT (Homologous to E6AP C-Terminus) E3 ubiquitin ligase family that regulates numerous cellular functions including inflammatory responses through T-cell activation, cell differentiation, and apoptosis. Known intracellular targets of ITCH-...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-018-9843-2
更新日期：2019-04-01 00:00:00
abstract：:X-linked inhibitor of apoptosis protein (XIAP), a leading member of the family of inhibitor of apoptosis (IAP) proteins, is considered as the most potent and versatile inhibitor of caspases and apoptosis. It has been reported that XIAP is frequently overexpressed in cancer and its expression level is implicated in con...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-009-9197-x
更新日期：2010-04-01 00:00:00
abstract：:In Northern America and Europe a great number of people are suffering from birch pollen allergy and pollen related food allergies. The trigger for these immunological reactions is the 17.5 kDa major birch pollen allergen Bet v 1, which belongs to the family of PR-10 (pathogenesis-related) proteins. In nature, Bet v 1 ...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-017-9754-7
更新日期：2017-10-01 00:00:00
abstract：:Salmonella typhimurium OppA is the periplasmic oligopeptide-binding protein. Backbone resonances of OppA(D419N) on its own were assigned for approximately 90% of residues. Missing residues are localised around the ligand-binding site, suggesting conformational flexibility in the unliganded state. ...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-007-9008-1
更新日期：2007-07-01 00:00:00
abstract：:Cohesin and dockerin domains are critical assembling components of cellulosome, a large extracellular multienzyme complex which is used by anaerobic cellulolytic bacteria to efficiently degrade lignocellulose. According to sequence homology, cohesins can be divided into three major groups, whereas cohesins from Clostr...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-012-9381-2
更新日期：2013-04-01 00:00:00
abstract：:Chromatin remodelling proteins are an essential family of eukaryotic proteins. They harness the energy from ATP hydrolysis and apply it to alter chromatin structure in order to regulate all aspects of genome biology. Chromodomain helicase DNA-binding protein 1 (CHD1) is one such remodelling protein that has specialise...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-015-9631-1
更新日期：2016-04-01 00:00:00
abstract：:The pathogenic bacterium Staphylococcus aureus has evolved to actively evade many aspects of the human innate immune system by expressing a series of secreted inhibitory proteins. Among these, the extracellular adherence protein (Eap) has been shown to inhibit the classical and lectin pathways of the complement system...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-016-9688-5
更新日期：2016-10-01 00:00:00
abstract：:Arsenate reductases (ArsC) are a group of enzymes that play essential roles in biological arsenic detoxification pathways by catalyzing the intracellular reduction of arsenate to arsenite, which is subsequently extruded from the cells by specific transport systems. The ArsC protein from cyanobacterium Synechocystis sp...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-010-9273-2
更新日期：2011-04-01 00:00:00
abstract：:Ebola viral infections have resulted in several deadly epidemics in recent years in West and Central Africa. Because only one of the seven proteins encoded by the viral genome possesses enzymatic activity, disruption of protein-protein interactions is a promising route for antiviral drug development. We carried out a ...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-019-09898-7
更新日期：2019-10-01 00:00:00
abstract：:Q38FZ4 (UniProt accession number), is an 85-residue hypothetical protein from Trypanosoma brucei (T. brucei). Q38FZ4, which might be specific among the trypanosomatid genomes, shares low sequence similarity with mammal proteins and also has an essential function in the growth of T. brucei. Here we report the resonance...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-020-09956-5
更新日期：2020-10-01 00:00:00
abstract：:We report the nearly complete 1H, 13C and 15N resonance assignments of the oxidized form (Cys(67)-Cys(70)) of the N-terminal domain of PilB from Neisseria meningitidis. Secondary structure determination using CSI method and TALOS leads mainly to the prediction of 7 alpha-helical and 5 beta-sheet parts. ...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-007-9038-8
更新日期：2007-07-01 00:00:00
abstract：:We report NMR assignments of the protein backbone of the C-terminal domain (163 a.a.) of human class 1 translation termination factor eRF1. It was found that several protein loop residues exist in two slowly interconverting conformational states. ...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-007-9050-z
更新日期：2007-12-01 00:00:00
abstract：:Norovirus protease cleaves the virus-encoded polyprotein into six mature nonstructural proteins, presenting itself as an essential enzyme for the viral replication as well as an attractive target for the antiviral drug development. A deeper understanding of the structural mechanism of the protease-substrates/inhibitor...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-011-9316-3
更新日期：2012-04-01 00:00:00
abstract：:Bacterial thiol peroxidases (Tpxs) are antioxidant enzymes which exist in various bacteria. Tpxs reduce the lipid hydroperoxides to protect the membrane lipid from destruction by reactive oxygen species. Tpxs are essential enzymes for bacterial anaerobic growth. Herein, we report the resonance assignments of (1)H, (13...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-008-9116-6
更新日期：2008-12-01 00:00:00
abstract：:During its life cycle, the malaria parasite Plasmodium falciparum is found intracellular to human erythrocytes, where its survival and ability to multiply critically depends on the control of the environment redox state. Thioredoxin is a small protein containing 104 amino acids that is part of the parasite specific re...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-009-9163-7
更新日期：2009-12-01 00:00:00
abstract：:LicT belongs to an essential family of bacterial antitermination proteins which bind to nascent mRNAs in order to stimulate transcription of sugar-metabolizing operons. As most of other antitermination proteins involved in carbohydrate metabolism, LicT is composed of a N-terminal RNA-binding module (CAT) and two homol...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-019-09914-w
更新日期：2020-04-01 00:00:00
abstract：:Human heterogeneous nuclear ribonucleoprotein H (hnRNP H) regulates alternative splicing of HIV-1 Tat pre-mRNA. The structure of the first N-terminal domain (residues 1-104) of hnRNP H was solved and its binding to an exonic splicing silencer (pESS2) studied. For this, all backbone and 85% of side-chain resonance freq...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-007-9061-9
更新日期：2007-12-01 00:00:00
abstract：:The catalytic subunit of protein kinase A is involved with a number of signal transduction pathways and has been used as a benchmark to study the structural biology and biochemistry for the entire kinase family of enzymes. Here, we report the backbone assignment of the intact 41 kDa catalytic subunit bound to AMP-PNP....
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-009-9154-8
更新日期：2009-06-01 00:00:00
abstract：:The gelation factor from Dictyostelium discoideum (ABP-120) is an actin binding protein consisting of six immunoglobulin (Ig) domains in the C-terminal rod domain. We have recently used the pair of domains 5 and 6 of ABP-120 as a model system for studying multi-domain nascent chain folding on the ribosome. Here we pre...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-008-9134-4
更新日期：2009-06-01 00:00:00
abstract：:The apo-form of the 21.4 kDa catalytic domain and the 10.7 kDa carbohydrate binding domain of the AA10 family lytic polysaccharide monooxygenase ScLPMO10C from Streptomyces coelicolor have been isotopically labeled and recombinantly expressed in Escherichia coli. In this paper, we report the 1H, 13C, and 15N chemical ...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-017-9759-2
更新日期：2017-10-01 00:00:00
abstract：:Fibroblast growth factor receptor (FGFR) 4 has been associated with progression of melanoma, breast, head and neck and hepatocellular carcinoma and is therefore an interesting target for therapeutic intervention (Ho et al. in J Hepatol 50:118-127, 2009). The extracellular D2 domain of the FGFR4 receptor contains a hep...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-012-9405-y
更新日期：2013-10-01 00:00:00
abstract：:RpsA, also known as ribosomal protein S1, is an essential protein required for translation initiation of mRNAs when their Shine-Dalgarno sequence is degenerated (Sorensen et al. 1998). In addition, RpsA of Mycobacterium tuberculosis (M. tb) is involved in trans-translation, which is an effective system mediated by tmR...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-017-9734-y
更新日期：2017-10-01 00:00:00
abstract：:Nuclear Factor κB (NF-κB) is a family of five related transcription factors that recognize a κB DNA element on the promoter and enhancer regions of target genes and modulate their expression. Here we report a complete set of 1H, 13C, 15N backbone and side chain resonance assignments for the p50 DNA binding and dimeriz...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-020-09978-z
更新日期：2020-09-16 00:00:00
abstract：:The homeobox gene (HOXA13) codes for a transcription factor protein that binds to AT-rich DNA sequences and controls expression of many important proteins during embryonic morphogenesis. We report complete NMR chemical shift assignments of the mouse HOXA13 DNA binding domain (A13DBD; BMRB no. 16252). ...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-009-9174-4
更新日期：2009-12-01 00:00:00
abstract：:KKT4 is a kinetoplastid-specific microtubule-binding kinetochore protein that lacks significant similarity to any known kinetochore or microtubule-binding proteins. Here we present the 1H, 13C and 15N resonance assignments for several fragments from the microtubule-binding domain of KKT4 (KKT4115-343) from Trypanosoma...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-020-09968-1
更新日期：2020-10-01 00:00:00
abstract：:Ras homologue enriched in brain (Rheb) is a small GTPase that plays an important role in tuberous sclerosis. Here we present the backbone assignments of activated rRheb in complex with the non-hydrolisable GTP analogue GppNHp. These assignments now provide a basis for the analysis of rRheb's interaction with putative ...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-007-9030-3
更新日期：2007-07-01 00:00:00
abstract：:Repressor of primer (Rop, or ROM, RNA I modulator) is a 63 amino acid four-helix bundle protein that exists in solution as an anti-parallel homodimer. This protein has been extensively studied, including by X-ray crystallography, NMR, rational design, and combinatorial mutagenesis. Previous NMR experiments with wild-t...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-018-9837-0
更新日期：2018-10-01 00:00:00
abstract：:Ubiquitin Specific Protease 25 (USP25), a member of the deubiquitinase family, is involved in several disease-related signal pathways including myogenesis, immunity and protein degradation. It specially catalyzes the hydrolysis of the K48-linked and K63-linked polyubiquitin chains. USP25 contains one ubiquitin-associa...
journal_title：Biomolecular NMR assignments
pub_type： 杂志文章
doi：10.1007/s12104-013-9495-1
更新日期：2014-10-01 00:00:00
