Abstract:
:In the presented study we analysed the effect of histone deacetylase inhibitors (HDACi) suberoylanilide hydroxamic acid (SAHA) and valproic acid (VPA) on human plasma cell leukemia (PCL) cell line UHKT-944 in the presence of bone marrow microenvironment (BMM). For the analysis, the cells were cultured alone, with bone marrow stromal cells (BMSCs), with extracellular matrix (ECM) components or with interleukin-6, and treated with varied concentrations of SAHA and VPA for 24/48 hours. To study the effect of HDACi, we investigated cell proliferation, apoptosis, cell cycle and changes in selected signalling pathways. We found that both SAHA and VPA induced apoptosis, but had no effect on the cell cycle distribution of UHKT-944 cells. Investigation of the antiproliferative effect of SAHA and VPA revealed that BMSCs and high concentration of interleukin-6 had partial protective effect against SAHA or both inhibitors, respectively. No effect of ECM components on the efficiency of HDACi was observed. We further revealed that VPA down-regulated STAT3 phosphorylation while both inhibitors decreased Akt phosphorylation. In conclusion, VPA and SAHA might represent an additional therapeutic strategy in the PCL treatment. Protective effect of BMM should be taken into account when investigating prospective therapeutic agents against plasma cell disorders.
journal_name
Neoplasmajournal_title
Neoplasmaauthors
Burianova I,Kuzelova K,Mitrovsky O,Spicka I,Stockbauer P,Zackova Mdoi
10.4149/neo_2017_209subject
Has Abstractpub_date
2017-01-01 00:00:00pages
228-237issue
2eissn
0028-2685issn
1338-4317journal_volume
64pub_type
杂志文章相关文献
NEOPLASMA文献大全abstract::Flavopiridol, a cyclin-dependent kinase inhibitor (CDKI), shows promising anti-tumor activity in hematologic malignancies. However, Flavopiridol-induced protective autophagy may lead to drug resistance. Here we found that Hsp90 inhibitor 17-AAG can sensitize mantle cell lymphoma (MCL) cells to flavopiridol by suppress...
journal_title:Neoplasma
pub_type: 杂志文章
doi:10.4149/neo_2015_047
更新日期:2015-01-01 00:00:00
abstract::Genetic background of high incidence of spontaneous tumors in BDX rats was investigated with respect to susceptibility to physical or chemical agents and immune response. Percentages of colony forming ability of normal embryo fibroblasts from BDX rats after exposure to various doses of ultraviolet (UV) light, X-rays a...
journal_title:Neoplasma
pub_type: 杂志文章
doi:
更新日期:1986-01-01 00:00:00
abstract::A report on 1877 consecutive node negative (N-) breast cancer cases is presented in which the prognostic value of the main clinico-pathologic features is evaluated. Tumor size (and UICC-TNM T category) and lobular infiltrating histologic type were significantly associated with a more favorable prognosis whereas no ass...
journal_title:Neoplasma
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract:PURPOSE:To explore the expression of the TRAV gene in peripheral blood mononuclear cells (PBMCs) and in tumor-infiltrating lymphocytes (TILs) in the patients with breast cancer using a DNA melting curve (FQ-PCR) technique for T cell receptor (TCR) alpha chain CDR3 spectratyping. Peripheral blood samples and tissue samp...
journal_title:Neoplasma
pub_type: 杂志文章
doi:10.4149/neo_2012_088
更新日期:2012-01-01 00:00:00
abstract::Functional microRNA (miRNA) molecules are transported in extracellular vesicles among tumor cells and cells of the immune system. Macrophages as integral components of tumor microenvironment are known as potential contributors to tumor growth and progression. We searched for studies which could provide a direct link b...
journal_title:Neoplasma
pub_type: 杂志文章
doi:10.4149/neo_2017_311
更新日期:2017-01-01 00:00:00
abstract::Serial cytogenetic characteristics of four lymphoblastoid cell lines derived from the peripheral blood of four patients with acute leukemia are presented, including G and C banding techniques and the analysis of sister chromatid exchanges by means of FPG technique. One of these lines turned into predominantly diploid ...
journal_title:Neoplasma
pub_type: 杂志文章
doi:
更新日期:1977-01-01 00:00:00
abstract::Protein p53 is the tumor suppressor involved in cell cycle control and apoptosis. As a transcription factor p53 controls many cell processes and helps in prevention of cancer development. The p53 gene is polymorphic. Polymorphisms can affect the important regions involved in protein tumor suppressor activity. The well...
journal_title:Neoplasma
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::Sixty-three locoregional breast cancer biopsies were examined for association among epidermal growth factor receptors (as parameter of autocrine growth control), and estrogen and progesterone receptors (as endocrine parameters of estrogen responsiveness). In the tumor group with similar steroid receptor levels, low (0...
journal_title:Neoplasma
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract::In contrast to the B77 virus, its B77(RBI) rat variant continuously produced by the rat RBI sarcoma cells in vivo and in vitro, efficiently induced progressively growing tumors in hamsters and transformed hamster embryo cells in vitro. Both, induced tumors and transformed cell cultures showed production of infectious ...
journal_title:Neoplasma
pub_type: 杂志文章
doi:
更新日期:1975-01-01 00:00:00
abstract::A 10-year old Nigerian boy was admitted to hospital with a history of swelling in the neck, change of voice, dysphagia and dyspnoea of 2 weeks duration. He died on the 2nd day of admission. Autopsy revealed a thymoma infiltrating the thyroid, trachea and neck muscles and transforming the tracheal lumen into a slit lik...
journal_title:Neoplasma
pub_type: 杂志文章
doi:
更新日期:1979-01-01 00:00:00
abstract::A modification of the "skin window" method is proposed for investigation of delayed hypersensitivity in patients with tumors. The principle of the modified method consists in an antigen injection into the site of lesion. Patients with tumors showed a strikingly lowered lymphoid cells and eosinophilic and basophilic gr...
journal_title:Neoplasma
pub_type: 杂志文章
doi:
更新日期:1976-01-01 00:00:00
abstract::In 41 patients with Hodgkin's disease nitroblue tetrazolium (NBT) dye reduction with resting and stimulated granulocytes, before, in the course and after chemotherapy was examined. Twofold decrease of the capability of NBT reduction test with stimulated granulocytes was found, whereas with resting granulocytes only sl...
journal_title:Neoplasma
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract::The experiments reported in this communication examined the immunological properties of cell lines cultured under (a) normal, physiological (Cont.Cal) and (b) experimental, non-physiological (Expt.Cal) conditions described in the preceeding paper. Determination of oncogenicity, humoral and cellular immunity of the cul...
journal_title:Neoplasma
pub_type: 杂志文章
doi:
更新日期:1980-01-01 00:00:00
abstract::Oral cancer is one of the common cancers worldwide, among which over 90% are oral squamous cell carcinomas (OSCC). MicroRNAs act as critical regulators of cancer development and progression. MiR-103a-3p has been reported to be upregulated in OSCC patients and closely correlated to poor prognosis, yet its roles in the ...
journal_title:Neoplasma
pub_type: 杂志文章
doi:10.4149/neo_2020_190430N382
更新日期:2020-05-01 00:00:00
abstract::Analysis of literary data and the author's findings have shown that the transplacental action of most of the compounds tested in experiments on rats manifested itself by a neurotropic carcinogenic effect. A marked neurotropism in transplacental carcinogenesis in rats is characteristic even for such drugs (e.g. dimethy...
journal_title:Neoplasma
pub_type: 杂志文章
doi:
更新日期:1976-01-01 00:00:00
abstract::Multiple ultrasonographic (USG) evaluations of abdomen were performed in 97 patients: 30 with Hodgkin's disease (HD), 60 with non-Hodgkin lymphomas (NHL) and 7 with primary gastric lymphoma (PGL) before or during chemo- or X-ray therapy. In 33% of HD patients USG was normal, while in 63% splenomegaly, in 40% hepatomeg...
journal_title:Neoplasma
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract::Lung cancer has been a major threat to human health worldwide. Vimentin (VIM) and E-cadherin (E-cad) are molecular markers that play important roles in epithelial-mesenchymal transition (EMT), which has been shown to be correlated with tumor progression. Herein, we investigated the associations between clinicopatholog...
journal_title:Neoplasma
pub_type: 杂志文章
doi:10.4149/neo_2017_506
更新日期:2017-01-01 00:00:00
abstract::The authors present results of a preliminary study on the efficiency of the preparation Penberol [VUFB Prague (cis-beta-4-pentoxy-benzoyl-beta-bromoacrylic acid) CHECO protocol number 4] in the ovarian carcinoma of the stage III and IV (FIGO) in which the drug was used after the failure of the first choice chemotherap...
journal_title:Neoplasma
pub_type: 杂志文章
doi:
更新日期:1980-01-01 00:00:00
abstract::Chromosomal aberrations in bone marrow and lymph node cells have been investigated in 47 patients with lymphogranulomatosis 24--72 hr after administration of a single therapeutic dose of cyclophosphan and natulan. It is shown that the injurious action of cyclophosphan and natulan lasts more than 72 hr. An analysis of ...
journal_title:Neoplasma
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract::In a group of 11,153 autopsied subjects a higher number of cholecystectomies was found in both sexes in individuals with large intestine tumors as compared with the rest of the autopsied persons without tumors (p less than 0.001). The results confirmed the hypothesis that cholecystectomy is a supporting factor in the ...
journal_title:Neoplasma
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract::We evaluated the prognostic impact of chromosomal abnormalities as detected by interphase fluorescence in situ hybridization (iFISH) in 86 chronic lymphocytic leukemia (CLL) patients. Overall, 39 of 86 (45%) patients displayed one (35%) or more (10%) chromosomal abnormalities, del13q (31%) being more frequently detect...
journal_title:Neoplasma
pub_type: 杂志文章
doi:10.4149/neo_2011_01_82
更新日期:2011-01-01 00:00:00
abstract::Forty-four cases of well documented progressive radiation myelopathy of thoracic spinal cord were analyzed in terms of the new DFT conception (Dose-Fraction-Time). The DFT conception is a new radiobiological cell tissue kinetic model comprising explicitly repopulation. The conception has been previously tested against...
journal_title:Neoplasma
pub_type: 杂志文章
doi:
更新日期:1986-01-01 00:00:00
abstract::Bladder cancer remains a very challenging disease to treat with the high rates of recurrence and progression associated with current therapies. Although the association between bladder cancer pathology and circRNAs remains undetermined, circRNAs signatures may be useful as prognostic and predictive factors and clinica...
journal_title:Neoplasma
pub_type: 杂志文章
doi:10.4149/neo_2019_190107N25
更新日期:2019-06-03 00:00:00
abstract::Carcinoma of oral cavity have a high risk of recurrence after initial treatment with surgery, radiotherapy, surgery with adjuvant radiotherapy, or radio-chemotherapy.The present study investigated the changes in expression, activity and regulation of matrix metalloproteinases (MMP) -2 and -9 in oral squamous cell carc...
journal_title:Neoplasma
pub_type: 杂志文章
doi:10.4149/neo_2014_091
更新日期:2014-01-01 00:00:00
abstract::A group of 54 patients with the original diagnosis of polycythemia vera were subjected to cytogenetic examination. Six (17.6%) of the 34 cases examined in the period of the advanced phase of the polycythemia vera had a chromosomal change. Thirteen (65%) of the 20 patients undergoing the cytogenetic examination in the ...
journal_title:Neoplasma
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is a particularly lethal form of cancer. Overall survival evenafter liver surgery is unsatisfactory due to high metastatic capacity and recurrence rates. Cancer stem cells (CSCs) were recently proposed to elucidate the molecular mechanism of HCC metastasis and recurrence. In our study, w...
journal_title:Neoplasma
pub_type: 杂志文章
doi:10.4149/neo_2015_068
更新日期:2015-01-01 00:00:00
abstract::We examined the effect of gamma-irradiation (4 Gy) alone or combined with estrogen (17beta-estradiol 15 microM) treatment on the radiation response of stromal fibroblasts from cervical tumors. The fibroblasts were derived from tumors of 9 younger (<50 years) and 9 older (>50 years) cervical cancer patients. A normal f...
journal_title:Neoplasma
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::Regional lymph nodes in 80 cases of gastric and 70 cases of breast cancer have been examined and the reactive types have been correlated to the survival periods of the patients. In both groups of patients, a prognostically favorable sign was the detection of lymphocytic predominance combined with sinus histiocytosis, ...
journal_title:Neoplasma
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract::Zinc finger protein 139(ZNF139), a member of zinc finger protein family, is a transcription factor. Our previous research showed ZNF139 was overexpressed in gastric cancer cells. The purpose of present study is to explore impact and mechanism of ZNF139 on metastasis by regulating invasive ability of gastric cancer cel...
journal_title:Neoplasma
pub_type: 杂志文章
doi:10.4149/neo_2014_037
更新日期:2014-01-01 00:00:00
abstract:UNLABELLED:The study was aimed at detecting the expression of a newly found oncogene, astrocyte elevated gene-1 (AEG-1), in renal cell carcinoma (RCC) and its correlation with histopathologic features and the survival of patients. Real-time reverse transcription-PCR and Western blot showed markedly higher expression of...
journal_title:Neoplasma
pub_type: 杂志文章
doi:10.4149/neo_2010_06_522
更新日期:2010-01-01 00:00:00