Screening specific polypeptides of breast cancer stem cells from a phage display random peptide library.


:The present study aimed to identify polypeptides that specifically bond to breast cancer stem cells from a phage display random 12 peptide library, in addition to the affinity and specificity of polypeptides. A phage display random 12 peptide library was screened using breast cancer stem cells as targets isolated from the MDA-MB-231 cell line using the serum-free culture technique with hs578bst and MDA-MB-231 cells as subtract-screening cells. Positive and specific binding clones were amplified and sent for sequencing. The affinity and specificity of the positive clones were subsequently identified by ELISA and 3,3'-diaminobenzidine staining. The results demonstrated that phages were gathered ~500 times following three rounds of biopanning. ELISA identified that the affinity to breast cancer stem cells of the no. 6 phage was 6.14 times higher than that in the control group. In addition, immunohistochemistry observed that the no. 6 phage exhibited high-specificity bonding to breast cancer stem cells, and the peptide sequence of the positive phage was GYSASRSTIPGK following DNA sequencing and translation. Thus, the present study isolated a specific peptide that bonds to breast cancer stem cells from a phage display random peptide library, which may facilitate further studies regarding the stem cell-targeted therapy of breast cancer.


Oncol Lett


Oncology letters


Liu F,Qi CL,Kong M,Liu TT,Li L,Li BJ




Has Abstract


2016-12-01 00:00:00














  • Effects of Platycodin D on apoptosis, migration, invasion and cell cycle arrest of gallbladder cancer cells.

    abstract::Platycodin D (PD) is a triterpenoid saponin that exists in the roots of Platycodonis. It exhibits evident growth inhibitory effects and potent cytotoxicity against multiple types of cancer. Gallbladder cancer (GBC) is the most common malignant disease of the biliary tract system. Patients with GBC usually have limited...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Zhang X,Zhai T,Hei Z,Zhou D,Jin L,Han C,Wang J

    更新日期:2020-12-01 00:00:00

  • Effects of melatonin on HIF-1α and VEGF expression and on the invasive properties of hepatocarcinoma cells.

    abstract::Liver cancer is the sixth most commonly occurring cancer globally, and the main histological type is hepatocellular carcinoma. This type of neoplasia has a poor prognosis due to a high rate of recurrence and intrahepatic metastasis, which are closely are closely associated with the angiogenic process. Vascular endothe...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Colombo J,Maciel JM,Ferreira LC,DA Silva RF,Zuccari DA

    更新日期:2016-07-01 00:00:00

  • Advanced prostatic ductal carcinoma in a patient with a long survival time following a total pelvis exenteration: A case report.

    abstract::Ductal adenocarcinoma of the prostate (DAP) is a rare variant of prostate cancer, and has poorly-identified clinical characteristics. Few cases have been previously reported in Chinese males and DAP is more commonly reported in Caucasian males over 70-years of age. In the present study, a 55-year-old Chinese male pati...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Ji C,Zhou M,Gan W,Zheng J,Yan X,Guo H

    更新日期:2016-02-01 00:00:00

  • Hyperthermia and radiation reduce the toxic side-effects of bufadienolides for cancer therapy.

    abstract::Bufadienolides are constituents of the traditional Chinese medicine Chan Su and are found in toad venom. Cardiovascular side-effects are one of the limiting factors towards developing bufadienolides as chemotherapeutic agents. Thus, in the present study, low doses of bufalin and cinobufotalin, prominent members of the...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Emam H,Refaat A,Jawaid P,Ur Rehman M,Li P,Zhao QL,Kondo T

    更新日期:2017-07-01 00:00:00

  • The distribution of human rectal aberrant crypt foci and criteria for defining the counting area.

    abstract::Aberrant crypt foci (ACF) are considered as a useful surrogate biomarker for colorectal cancer, although their biological significance still remains controversial. We conducted this study to clarify whether differences in the ACF counting area might have led to the discrepancies in the ACF counts among previous report...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Fujii T,Sakai E,Takahashi H,Yamada E,Ohkubo H,Higurashi T,Nakajima A

    更新日期:2017-06-01 00:00:00

  • Letrozole as second-line hormonal treatment for recurrent low-grade endometrial stromal sarcoma: A case report and review of the literature.

    abstract::Low-grade endometrial stromal sarcoma (LGESS) is a rare malignancy. The tumor is reportedly responsive to hormonal therapy, most commonly with medroxyprogesterone acetate (MPA), but the effectiveness of aromatase inhibitors for recurrent LGESS remains unclear. The present study reports a case of stage IC LGESS present...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Nakamura K,Nakayama K,Ishikawa M,Ishikawa N,Katagiri H,Katagiri A,Ishibashi T,Sato E,Iida K,Sultana R,Kyo S

    更新日期:2016-11-01 00:00:00

  • Effect of CCR7, CXCR4 and VEGF-C on the lymph node metastasis of human pancreatic ductal adenocarcinoma.

    abstract::The aim of the present study was to investigate the association between the expression of chemokine receptors CCR7 and CXCR4 and vascular endothelial growth factor (VEGF)-C and the lymph node metastasis of pancreatic ductal adenocarcinoma (PDAC). The mRNA transcription levels of CCR7, CXCR4 and VEGF-C were measured in...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Guo J,Lou W,Ji Y,Zhang S

    更新日期:2013-05-01 00:00:00

  • Gemcitabine and cisplatin-induced tumor lysis syndrome in a patient with gallbladder carcinoma: A case report.

    abstract::We report a case of tumor lysis syndrome (TLS) in a patient with gallbladder carcinoma. TLS has not been reported in association with this type of tumor. TLS typically occurs in cases of highly proliferative hematological malignancies and small cell carcinoma. Two factors that may have contributed to TLS in this case ...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Duff DJ,Haddadin S,Freter C,Papageorgiou C

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  • Expression of cluster of differentiation 34 and vascular endothelial growth factor in breast cancer, and their prognostic significance.

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    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Chen Z,Xu S,Xu W,Huang J,Zhang GU,Lei L,Shao X,Wang X

    更新日期:2015-08-01 00:00:00

  • Effect of recombinant Newcastle disease virus transfection on lung adenocarcinoma A549 cells in vivo.

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    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Yan Y,Jia L,Zhang J,Liu Y,Bu X

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  • High expression level of T-box transcription factor 5 predicts unfavorable survival in stage I and II gastric adenocarcinoma.

    abstract::The expression of T-box transcription factor 5 (TBX5) has previously been observed in human cancer. The aim of the present study was to investigate TBX5 expression and its potential clinical significance in gastric cancer (GC). Using reverse transcription-quantitative polymerase chain reaction, the TBX5 mRNA expressio...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Zheng Y,Li YF,Wang W,Chen YM,Wang DD,Zhao JJ,Pan QZ,Jiang SS,Zhang XF,Yuan SQ,Qiu HB,Huang CY,Zhao BW,Zhou ZW,Xia JC

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  • Triptolide induces protective autophagy and apoptosis in human cervical cancer cells by downregulating Akt/mTOR activation.

    abstract::Triptolide exhibits immunosuppressive, anti-inflammatory, antifertility and antineoplastic functions. However, the anticancer effect of triptolide on cervical cancer and the underlying mechanism remains to be fully understood. The present study assessed the mechanisms underlying the effect of triptolide on the viabili...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Qin G,Li P,Xue Z

    更新日期:2018-09-01 00:00:00

  • Primary paratesticular osteosarcoma: A case report.

    abstract::Extraosseous osteosarcoma originating from paratesticular soft tissue is an extremely rare type of malignant tumor. With the exception of the present study, only one other case of osteosarcoma originating from paratesticular soft tissue has previously been reported. A 52-year-old man presented with a painless scrotal ...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Hong R,Lee G,Kim H,Kim CS,Kee KH

    更新日期:2012-03-01 00:00:00

  • Calcium gluconate alleviates the toxic effect of hydrofluoric acid on human dermal fibroblasts through the Wnt/β-catenin pathway.

    abstract::The present study was performed to determine the molecular mechanism of calcium gluconate (CG) in alleviating the toxic effect of hydrofluoric (HF) acid on human dermal fibroblasts (HDFs). HDF morphology was observed by optical microscopy and the vimentin immunofluorescence assay. Cell viability and apoptosis were eva...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Peng J,Liu R,Peng L,Jia H

    更新日期:2018-09-01 00:00:00

  • Downregulation of miR-19a inhibits the proliferation and promotes the apoptosis of osteosarcoma cells by regulating the JAK2/STAT3 pathway.

    abstract::Osteosarcoma is a malignant tumor derived from the skeletal system, often occurring in bone tissues, and it is the most common malignant tumor in the skeletal system, with more than 90% of cases being highly malignant. The present study was designed to explore the regulatory effects of microRNA (miR)-19a on the prolif...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Chen J,Chen Z

    更新日期:2020-11-01 00:00:00

  • Downregulation of regulator of G protein signaling 2 expression in breast invasive carcinoma of no special type: Clinicopathological associations and prognostic relevance.

    abstract::Changes in the expression of regulator of G protein signaling 2 (RGS2) are involved in the genesis and development of a number of malignancies. However, the association between changes in the expression of RGS2 and breast invasive carcinoma of no special type (BIC-NST) remains unknown. The present study found that, in...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Wang C,Ye Q,Cao Y,Tan J,Wang F,Jiang J,Cao Y

    更新日期:2018-01-01 00:00:00

  • DHFR and MDR1 upregulation is associated with chemoresistance in osteosarcoma stem-like cells.

    abstract::Tumor-initiating cells (TICs) are defined as a specialized subset of cells with tumor-initiating capacity that can initiate tumor growth, tumor relapse and metastasis. In the present study, osteosarcoma TICs (OS-TICs) were isolated and enriched from the osteosarcoma U2OS and MG-63 cell lines using sphere formation ass...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Lee YH,Yang HW,Yang LC,Lu MY,Tsai LL,Yang SF,Huang YF,Chou MY,Yu CC,Hu FW

    更新日期:2017-07-01 00:00:00

  • S100A11 promotes human pancreatic cancer PANC-1 cell proliferation and is involved in the PI3K/AKT signaling pathway.

    abstract::S100A11, a member of S100 calcium-binding protein family, is associated with the numerous processes of tumorigenesis and metastasis. In the present study, the role of S100A11, and its possible underlying mechanisms in cell proliferation, apoptosis and cell cycle distribution in human pancreatic cancer were explored. I...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Xiao M,Li T,Ji Y,Jiang F,Ni W,Zhu J,Bao B,Lu C,Ni R

    更新日期:2018-01-01 00:00:00

  • Abundant circulating microRNAs in breast cancer patients fluctuate considerably during neoadjuvant chemotherapy.

    abstract::Previous studies have revealed the aberrant expression of a number of microRNAs (miRNA/miRs) in the blood circulation of patients with breast cancer (BC). The aim of the present study was to assess the effect of neoadjuvant chemotherapy on the levels of a panel of BC-associated miRNAs, which are at relatively low (let...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Gezer U,Keskin S,Iğci A,Tükenmez M,Tiryakioğlu D,Cetinkaya M,Dişci R,Dalay N,Eralp Y

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  • CAR-T 19 combined with reduced-dose PD-1 blockade therapy for treatment of refractory follicular lymphoma: A case report.

    abstract::Anti-CD19 chimeric antigen receptor T cell (CAR-T) therapy has changed the typical outcomes of relapsed/refractory B-cell leukemia and lymphoma. However, treatment effectiveness for patients with relapsed/refractory B-cell non-Hodgkin lymphoma has been less satisfactory compared with patients with B-cell acute lymphob...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Wang J,Deng Q,Jiang YY,Zhang R,Zhu HB,Meng JX,Li YM

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  • Differential expressions of integrin-linked kinase, β-parvin and cofilin 1 in high-fat diet induced prostate cancer progression in a transgenic mouse model.

    abstract::High-fat diet induced obesity was associated with more aggressive prostate cancer. Recent research has demonstrated that integrin-linked kinase (ILK), β-parvin and downstream cofilin 1 jointly affected cancer progression. Meanwhile, these proteins were also involved in energy metabolism. Therefore, the present study w...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Hu MB,Hu JM,Jiang LR,Yang T,Zhu WH,Hu Y,Wu XB,Jiang HW,Ding Q

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  • Piper betle extracts exhibit antitumor activity by augmenting antioxidant potential.

    abstract::The present study was conducted to evaluate the methanolic extract of Piper betle leaves (MPBL) and its organic fractions with regard to antitumor activity against Ehrlich ascites carcinoma (EAC) in Swiss albino mice and to confirm their antioxidant activities. At 24 h post-intraperitoneal inoculation of tumor cells i...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Alam B,Majumder R,Akter S,Lee SH

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  • Multifocal epithelial hyperplasia: A potentially precancerous disease? (Review).

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    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Bascones-Martínez A,Cok S,Bascones-Ilundáin C,Arias-Herrera S,Gomez-Font R,Bascones-Ilundáin J

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  • Exosomes derived from HBV-associated liver cancer promote chemoresistance by upregulating chaperone-mediated autophagy.

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    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Liu DX,Li PP,Guo JP,Li LL,Guo B,Jiao HB,Wu JH,Chen JM

    更新日期:2019-01-01 00:00:00

  • Thermal ablation in cancer.

    abstract::Radiofrequency ablation (RFA) and cryoablation are alternative forms of therapy used widely in various pathological states, including treatment of carcinogenesis. The reason is that ablation techniques have ability of modulating the immune system. Furthermore, recent studies have applied this form of therapy on tumor ...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Liu Y,Cao CS,Yu Y,Si YM

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  • Familial genetic tuberous sclerosis complex associated with bilateral giant renal angiomyolipoma: A case report.

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    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Wang L,Ni D,Zhong L,Wang J

    更新日期:2017-12-01 00:00:00

  • Correlation between CT features of adrenocortical and adrenal medullary tumors and expression of miR-96 in serum.

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    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Xu Y,Wang Z,Bi Y,Duan Z,Yue X

    更新日期:2018-08-01 00:00:00

  • DNA damage regulated autophagy modulator 1 recovers the function of apoptosis-stimulating of p53 protein 2 on inducing apoptotic cell death in Huh7.5 cells.

    abstract::Overexpression of apoptosis-stimulating of p53 protein 2 (ASPP2) can induce apoptotic cell death in hepatoma cells, which contributes to a killing effect of ASPP2 on treating hepatocellular carcinoma (HCC). In the present study, ASPP2 overexpression failed to induce apoptotic cell death in the HCC Huh7.5 cell line, bu...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Liu D,Li R,Guo X,Pang L,Zang Y,Liu K,Chen D

    更新日期:2018-06-01 00:00:00

  • Inhibition of tumor necrosis factor-α enhances apoptosis induced by nuclear factor-κB inhibition in leukemia cells.

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    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Dong QM,Ling C,Chen X,Zhao LI

    更新日期:2015-12-01 00:00:00

  • Telomeres and telomerase in oncogenesis.

    abstract::Telomeres are located at the ends of chromosomes and protect them from degradation. Suppressing the activity of telomerase, a telomere-synthesizing enzyme, and maintaining short telomeres is a protective mechanism against cancer in humans. In most human somatic cells, the expression of telomerase reverse transcriptase...

    journal_title:Oncology letters

    pub_type: 杂志文章,评审


    authors: Trybek T,Kowalik A,Góźdź S,Kowalska A

    更新日期:2020-08-01 00:00:00