Screening specific polypeptides of breast cancer stem cells from a phage display random peptide library.


:The present study aimed to identify polypeptides that specifically bond to breast cancer stem cells from a phage display random 12 peptide library, in addition to the affinity and specificity of polypeptides. A phage display random 12 peptide library was screened using breast cancer stem cells as targets isolated from the MDA-MB-231 cell line using the serum-free culture technique with hs578bst and MDA-MB-231 cells as subtract-screening cells. Positive and specific binding clones were amplified and sent for sequencing. The affinity and specificity of the positive clones were subsequently identified by ELISA and 3,3'-diaminobenzidine staining. The results demonstrated that phages were gathered ~500 times following three rounds of biopanning. ELISA identified that the affinity to breast cancer stem cells of the no. 6 phage was 6.14 times higher than that in the control group. In addition, immunohistochemistry observed that the no. 6 phage exhibited high-specificity bonding to breast cancer stem cells, and the peptide sequence of the positive phage was GYSASRSTIPGK following DNA sequencing and translation. Thus, the present study isolated a specific peptide that bonds to breast cancer stem cells from a phage display random peptide library, which may facilitate further studies regarding the stem cell-targeted therapy of breast cancer.


Oncol Lett


Oncology letters


Liu F,Qi CL,Kong M,Liu TT,Li L,Li BJ




Has Abstract


2016-12-01 00:00:00














  • Preliminary investigation of the role of BTB domain-containing 3 gene in the proliferation and metastasis of hepatocellular carcinoma.

    abstract::Hepatocellular carcinoma (HCC) is one of the leading digestive malignancies, with a high metastasis and recurrence. The development and rapid progression of HCC involves numerous complex molecular and cellular events. Therefore, developing effective methods for the prevention and treatment of HCC requires an improved ...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Xiao W,Zhao W,Li L,Wu Q,Zhu L,Zhang Q,Dai W,Wang Y,Zhang B

    更新日期:2017-08-01 00:00:00

  • Identifying prognosis and metastasis-associated genes associated with Ewing sarcoma by weighted gene co-expression network analysis.

    abstract::Ewing sarcoma (ES) is a highly malignant pediatric tumor with a low survival rate and a high rate of metastasis. However, there have been limited reports on the exploration of new biomarkers of ES. Therefore, the aim of the present study was to identify the potential hub genes associated with overall vital survival (O...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Wang B,Li J,Li X,Ou Y

    更新日期:2019-10-01 00:00:00

  • Effects of silencing RIP1 with siRNA on the biological behavior of the LoVo human colon cancer cell line.

    abstract::The present study aimed to investigate the effects of silencing RIP1 by small interfering RNA (siRNA) on the biological behavior of the LoVo human colorectal carcinoma cell line and to provide evidence for the feasibility of colorectal cancer gene therapy. LoVo cells were divided into the RIP1 siRNA group, the blank c...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: You HX,Zhou YH,Tan SY,She TH

    更新日期:2014-06-01 00:00:00

  • Primary gastrointestinal stromal tumor of the prostate: A case report and literature review.

    abstract::Extragastrointestinal stromal tumors (EGISTs) are relatively rare soft tissue neoplasms arising from the extra gastrointestinal tract. The current study presents a case of primary EGIST of the prostate observed in a 55-year-old male. Imaging studies showed a 10×10.5×9.5-cm prostate mass. On histological observation, t...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Liu S,Yu Q,Han W,Qi L,Zu X,Zeng F,Xie Y,Liu J

    更新日期:2014-06-01 00:00:00

  • In vitro stimulation of calcium overload and apoptosis by sonodynamic therapy combined with hematoporphyrin monomethyl ether in C6 glioma cells.

    abstract::The present study investigated enhancement of apoptosis induction and the mechanisms underlying calcium overload on C6 glioma cells in vitro, stimulated by low-level ultrasound in combination with hematoporphyrin monomethyl ether (HMME). The optimum frequency of ultrasound was determined by 3-(4,5-dimethythiazol-2-yl)...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Dai S,Xu C,Tian Y,Cheng W,Li B

    更新日期:2014-10-01 00:00:00

  • Primary pulmonary leiomyosarcoma: A case report.

    abstract::Primary pulmonary leiomyosarcoma (PPL) is an extremely rare malignant tumor. It has been revealed that PPL may originate from the smooth muscle of the pulmonary parenchyma, pulmonary arteries and bronchi. Patients with PPL may be asymptomatic or present with symptoms similar to those observed in other primary lung tum...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Xie X,Chen Y,Ding C,Yu X,Zou L,Xu B,Wang L,Huang X

    更新日期:2016-03-01 00:00:00

  • Synergistic effect of bladder cancer-specific oncolytic adenovirus in combination with chemotherapy.

    abstract::Gene therapy with adenoviral early region gene (E1A) may enhance the susceptibility of neoplastic cells to chemotherapy-induced cell death. Our previous study developed a urothelium-specific oncolytic serotype 5 adenovirus (Ad5) with the uroplakin II (UPII) promoter controlling E1A expression. The present study invest...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Li S,Wang F,Zhai Z,Fu S,Lu J,Zhang H,Guo H,Hu X,Li R,Wang Z,Rodriguez R

    更新日期:2017-08-01 00:00:00

  • Expression of V-domain immunoglobulin suppressor of T cell activation is associated with the advanced stage and presence of lymph node metastasis in ovarian cancer.

    abstract::V-domain immunoglobulin suppressor of T cell activation (VISTA) is a novel negative immune checkpoint that belongs to the B7 family. VISTA is primarily expressed on hematopoietic cells and inhibits T cell proliferation and cytokine production. The blockade of VISTA has demonstrated promising results in certain murine ...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Liao H,Zhu H,Liu S,Wang H

    更新日期:2018-09-01 00:00:00

  • Imaging and pathological features of primary hepatic neuroendocrine carcinoma: An analysis of nine cases and review of the literature.

    abstract::The present study aimed to analyze the imaging features and pathological basis of primary hepatic neuroendocrine carcinoma (PHNEC). A retrospective analysis of the imaging and pathological features of nine PHNEC cases was carried out at The Second Xiangya Hospital of Central South University (Changsha, China). The nin...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Chen Z,Xiao HE,Ramchandra P,Huang HJ

    更新日期:2014-04-01 00:00:00

  • Mutations of the TERT promoter are associated with aggressiveness and recurrence/distant metastasis of papillary thyroid carcinoma.

    abstract::Several previous studies have shown that mutations in B-Raf proto-oncogene (BRAF) and telomerase reverse transcriptase (TERT) can be used for the diagnosis and prognosis of papillary thyroid carcinoma (PTC). However, whether mutations in BRAF and the TERT promoter may improve the accurate identification and risk strat...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Liu R,Li Y,Chen W,Cong J,Zhang Z,Ma L,Chu L,Xiao H,Zhang Y,Liu Y,Xu Y,Yu Q,Yang X,Sun C

    更新日期:2020-10-01 00:00:00

  • Inflammation: A key process in skin tumorigenesis.

    abstract::The extremely delicate shift from an inflammatory process to tumorigenesis is a field of major scientific interest. While the inflammation induced by environmental agents has well known underlying mechanisms, less is known concerning the oncogenic changes that follow an inflammatory chronic status in the tissue microe...

    journal_title:Oncology letters

    pub_type: 杂志文章,评审


    authors: Neagu M,Constantin C,Caruntu C,Dumitru C,Surcel M,Zurac S

    更新日期:2019-05-01 00:00:00

  • Association between 5-lipoxygenase expression, and malignant behaviors and poor prognosis in esophageal squamous cell carcinoma.

    abstract::5-lipoxygenase (5-LO) catalyzes the first step of arachidonic acid metabolism to inflammatory mediator leukotrienes. The present study assessed 5-LO expression in esophageal squamous cell carcinoma (ESCC) tissue specimens for associations with clinicopathological and survival data from patients, then explored 5-LO act...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Bai CY,Zhang JY,Shi TW,Bai YQ,Wu BL,Du ZP,Wu ZY,Xu XE,Wang SH,Wu JY,Te RY,Zhang JY,Xu LY,Li EM

    更新日期:2018-06-01 00:00:00

  • Melanotic neuroectodermal tumor of infancy: A case report and review of the surgical treatment.

    abstract::Melanotic neuroectodermal tumor of infancy (MNTI) is a rare, fast-growing, benign tumor originating from the neural crest. The tumor most often occurs during the first year of life. The predilection site of MNTI is the anterior maxilla, whereas lesions of the mandible are uncommon and account for only 6% of all cases....

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Cui Y,Mao Z,Liao C

    更新日期:2015-01-01 00:00:00

  • Treatment of an Askin tumor: A case report and review of the literature.

    abstract::Askin tumors are rare malignant neoplasms that are localized in the thoracopulmonary region and mainly occur in children and adolescents. Further investigation with regard to the effective treatment of this disease is required, since the disease has a low incidence and limited knowledge is available on the biological ...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Dou X,Yan H,Wang R

    更新日期:2013-10-01 00:00:00

  • Ultraviolet light exposure, skin cancer risk and vitamin D production.

    abstract::The danger of overexposure to solar ultraviolet radiation has been widely reviewed since the 1980s due to the depletion of the ozone layer. However, the benefits of mild exposure of the skin to ultraviolet (UV) light have not been widely investigated. Numerous reports have demonstrated that an association exists betwe...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Rivas M,Rojas E,Araya MC,Calaf GM

    更新日期:2015-10-01 00:00:00

  • Genetic analysis and phosphoinositide 3-kinase/protein kinase B signaling pathway status in ovarian endometrioid borderline tumor samples.

    abstract::Ovarian endometrioid borderline tumors (EBTs) are extremely rare, and are thought to be precursors of endometrioid carcinoma, beginning as adenofibroma or endometriosis and progressing in a slow, stepwise manner. In endometrioid carcinomas, a high frequency of activating mutations in phosphatase and tensin homolog (PT...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Nakamura K,Nakayama K,Ishikawa M,Minamoto T,Ishibashi T,Sato E,Sanuki K,Yamashita H,Ono R,Iida K,Sultana R,Hossain MM,Ishikawa N,Kyo S

    更新日期:2018-07-01 00:00:00

  • MicroRNA and endometrial cancer: Roles of small RNAs in human tumors and clinical applications (Review).

    abstract::MicroRNAs (miRNAs) are small non-coding RNAs of approximately 22 base pairs that regulate the expression of genes by targeting messenger RNA with complementarity with the miRNA base sequence. Regulation of gene expression by miRNAs is crucial in cellular development and differentiation, and recent studies suggest a re...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Yanokura M,Banno K,Kobayashi Y,Kisu I,Ueki A,Ono A,Masuda K,Nomura H,Hirasawa A,Susumu N,Aoki D

    更新日期:2010-11-01 00:00:00

  • Talin2 regulates invasion of human breast cancer MDA-MB-231 cells via alteration of the tumor microenvironment.

    abstract::The talin proteins are a key component of the extracellular matrix-integrin-cytoskeleton system, and our previous study suggested that talin2 contributes to the tumor invasion and metastasis processes regulated by the tumor microenvironment. In the present study, the specific effects of talin2 on the invasive ability ...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Wen Z,Liang Y,Deng S,Zou L,Xie X,Yang J,Wu Y

    更新日期:2019-06-01 00:00:00

  • Ex vivo hepatectomy and partial liver autotransplantation for hepatoid adenocarcinoma: A case report.

    abstract::We previously reported the case of a 56-year-old male who underwent surgical treatment for gastric hepatoid adenocarcinoma and splenic metastasis. The present study reports the case of the same patient who underwent successful ex vivo hepatectomy and partial liver autotransplantation. Computed tomography scans demonst...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Wang FQ,Lu Q,Yan J,Peng YY,Xie CR,Su YJ,Zhou JY,Wang BL,Zhao WX,Wang XM,Bie P,Yin ZY

    更新日期:2015-05-01 00:00:00

  • Pneumocystis jiroveci pneumonia and colonization in patients with advanced lung cancer.

    abstract::Pneumocystis jiroveci pneumonia (PCP) has long been recognized as a cause of mortality in immuno-compromised populations, including those with advanced lung cancer. Although Pneumocystis colonization has only recently been described due to the development of more sensitive molecular techniques, including polymerase ch...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Togashi Y,Masago K,Ito Y,Sakamori Y,Okuda C,Fukuhara A,Nagai H,Kim YH,Mishima M

    更新日期:2013-02-01 00:00:00

  • Establishment of an insufficient radiofrequency ablation orthotopic nude mouse model of hepatocellular carcinoma to study the invasiveness and metastatic potential of residual cancer.

    abstract::In order to assess the metastatic potential of residual hepatocellular carcinoma (HCC) following insufficient radiofrequency ablation (RFA) and to improve the current animal model, an insufficient RFA orthotopic nude mouse model of HCC was developed in the present study. A human HCC orthotopic nude mouse model was est...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Zhang N,Zhu H,Dong YH,Wang L

    更新日期:2019-09-01 00:00:00

  • The mechanism of and the association between interleukin-27 and chemotherapeutic drug sensitivity in lung cancer.

    abstract::Interleukins (ILs) are involved in the occurrence and development of numerous types of cancer, and serve a critical role in the development of effective cancer therapeutics. The aim of the present study was to investigate the effect of IL-27 on chemotherapy resistance in lung cancer cells, and analyze its potential mo...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Jiang B,Shi W,Li P,Wu Y,Li Y,Bao C

    更新日期:2021-01-01 00:00:00

  • Therapy with transcutaneous administration of imiquimod combined with oral administration of sorafenib suppresses renal cell carcinoma growing in an orthotopic mouse model.

    abstract::Imiquimod is an imidazoquinoline immune response modifier that is used in antiviral and antiallergic creams. Combination therapy using transcutaneous imiquimod and oral sorafenib was previously demonstrated to reduce the tumor burden of renal cell carcinoma growing cutaneously in a mouse model. In the present study, a...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Karashima T,Udaka K,Niimura M,Suzuki K,Osakabe H,Shimamoto T,Fukata S,Inoue K,Kuroda N,Seiki M,Shuin T

    更新日期:2017-07-01 00:00:00

  • Long non-coding RNA growth arrest-specific transcript 5 in tumor biology.

    abstract::The recognition of the biological relevance of long non-coding RNA (lncRNA) molecules has only recently been recognized as one of the most significant advances in contemporary molecular biology. A growing body of evidence indicates that lncRNAs act not only as the intermediary between DNA and protein but also as signi...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Yu X,Li Z

    更新日期:2015-10-01 00:00:00

  • Serum concentration and expression of Reg IV in patients with esophageal cancer: Age-related elevation of serum Reg IV concentration.

    abstract::Regenerating islet-derived family, member 4 (REG4, which encodes Reg IV) is a marker for cancer and inflammatory bowel disease. This study aimed to investigate the diagnostic utility of Reg IV measurement in sera from esophageal cancer patients. Reg IV expression was examined in 269 esophageal cancer samples by immuno...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Oue N,Noguchi T,Anami K,Sentani K,Sakamoto N,Uraoka N,Wakamatsu Y,Sasaki H,Yasui W

    更新日期:2011-03-01 00:00:00

  • Aryl hydrocarbon receptor nuclear translocator promotes the proliferation and invasion of clear cell renal cell carcinoma cells potentially by affecting the glycolytic pathway.

    abstract::Aryl hydrocarbon receptor nuclear translocator (ARNT) is a transcription factor that has been reported to play a vital role in regulating glycolysis, angiogenesis and apoptosis. Recently, ARNT has been reported to a play role in pancreatic-islet function in type 2 diabetes. However, the role of ARNT in kidney cancer h...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Zhao Y,Han F,Zhang X,Zhou C,Huang D

    更新日期:2020-10-01 00:00:00

  • Inhibition of Ep3 attenuates migration and promotes apoptosis of non-small cell lung cancer cells via suppression of TGF-β/Smad signaling.

    abstract::Non-small cell lung cancer (NSCLC) is the most common cause of cancer-associated mortality worldwide. Prostaglandin E2 (PGE2) regulates various biological processes, including invasion, proliferation and apoptosis. E-prostanoid 3 (Ep3) is a PGE2 receptor, and the functional role of Ep3 in the progression of NSCLC rema...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Li L,Lv Y,Yan D

    更新日期:2018-11-01 00:00:00

  • Trends of incidence and survival in patients with gastrointestinal mucinous adenocarcinoma.

    abstract::The epidemiology and clinical outcome of gastrointestinal mucinous adenocarcinoma (MA) are not well illustrated. The present study aimed to explore the evolving epidemiology and prognostic factors that affect the survival of patients with MA in the gastrointestinal tract. A retrospective and population-based study was...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Zong Z,Luo Y,Ying H,Wang A,Li H,Yi C

    更新日期:2018-11-01 00:00:00

  • Analysis of soluble urokinase plasminogen activator receptor in multiple myeloma for predicting prognosis.

    abstract::Multiple myeloma is a type of malignancy, which affects the plasma cells of the bone marrow. Recent studies have found that malignant plasma cells may express urokinase plasminogen activator (uPA) and uPA receptor (uPAR), and that initiation of proteolytic events by this system contributes to the process of invasion a...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Shen J,Wang Q,Wang J,Su GH,Wang J,Guo SH,Liu YA,Wu Z,Liu RF,Li X,Guo XJ,Cao J,Zhang YH,Wang ZY

    更新日期:2015-10-01 00:00:00

  • Clear cell myoepithelial carcinoma in the base of the tongue: Case report and review of the literature.

    abstract::Clear cell myoepithelial carcinoma is a very rare disease which develops primarily in the parotid gland. To date, only 17 cases of clear cell myoepithelial carcinoma have been reported worldwide. Among them, only three cases developed in the minor salivary gland in the oral cavity. No cases developed in the base of th...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Park JH,Choi JY,Hong R,DO NY

    更新日期:2012-12-01 00:00:00