Abstract:
CONTEXT:High-mobility group box 1 (HMGB1) protein is a highly abundant protein that can promote the pathogenesis of inflammatory. Some experiments have demonstrated a vital role for HMGB1 to modulate the immune function of regulatory T-cells (Tregs). Astragaloside IV (AST IV), an extract from Astragalus membranaceus Moench (Leguminosae), has been shown to exert potent cardioprotective and anti-inflammatory effects. It is still unclear whether AST IV has a latent effect on the proinflammatory ability of HMGB1 with subsequent activation of Tregs in vivo. OBJECTIVE:This research explores the antagonism of different doses of AST IV on the immunologic function of Tregs mediated by HMGB1. MATERIALS AND METHODS:Mouse models (BALB/c) were constructed by which normal saline or AST IV was administered i.p. at 2, 4 and 6 days after the administration i.p. of 20 μg recombinate HMGB1. Spleen was used to procure Treg and CD4 + CD25- T-cells which were co-cultured with Treg. Cell phenotypes of Tregs(Foxp3) were examined, and the cytokine levels in supernatants and the proliferation of T-cells were assayed. Gene expression was measured by RT-PCR. RESULTS:(1) The expression levels of Foxp3 in Treg on post-stimulus days (PSD) 1-7 were significantly decreased in the HMGB1 group in comparison to those in the control group mice (p < 0.01). The Foxp3 expression was markedly increased in a dose-dependent manner in the AST group as compared with those in the HMGB1 group (p < 0.0 1-0.05). The same results were found in the contents of cytokines (IL-10 and TGF-β) released into supernatants by Treg. (2) When CD4 + CD25- T-cells were co-cultured with Treg stimulated by HMGB1, the cell proliferation and the levels of cytokines (IL-2 and IFN-γ) in supernatant were markedly increased as compared with those in the HMGB1 group. The level of IL-4 was markedly decreased as compared with that in the HMGB1 group. The same results were found when CD4 + CD25- T-cells were co-cultured with Treg in the NS group. Compared with those in the NS group, the contrary results were shown in a dose-dependent manner in the AST group. DISCUSSION AND CONCLUSION:These results showed that AST IV has a therapeutic effect on inflammation promoted by HMGB1, and it should be studied as a new drug for the treatment of sepsis.
journal_name
Pharm Bioljournal_title
Pharmaceutical biologyauthors
Li J,Huang L,Wang S,Yao Y,Zhang Zdoi
10.1080/13880209.2016.1216133subject
Has Abstractpub_date
2016-12-01 00:00:00pages
3217-3225issue
12eissn
1388-0209issn
1744-5116journal_volume
54pub_type
杂志文章abstract:CONTEXT:Cancer chemopreventive action of walnut [Juglans regia L. (Juglandaceae)] has been explored. OBJECTIVE:This study evaluated antiproliferative and antioxidant activities of walnut. MATERIALS AND METHODS:Various fractions of walnut extract have been screened for antiproliferative activity against human cancer c...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2010.537666
更新日期:2011-06-01 00:00:00
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journal_title:Pharmaceutical biology
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更新日期:2017-12-01 00:00:00
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journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2014.920396
更新日期:2015-03-01 00:00:00
abstract::Proteins from Parkia speciosa Hassk. (Fabaceae) seeds were extracted and stepwise precipitated using ammonium sulfate. Proteins precipitated with 25% ammonium sulfate were separated by affinity chromatography on Affi-Gel Blue gel followed by protein liquid chromatography on Superdex 200. The protein Gj, which was iden...
journal_title:Pharmaceutical biology
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journal_title:Pharmaceutical biology
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doi:10.3109/13880209.2012.698288
更新日期:2013-01-01 00:00:00
abstract:CONTEXT:Clerodendrum viscosum Vent. (Verbenaceae) is a shrub, widely used amongst the natives of India against various diseases. OBJECTIVE:Crude extract of the plant was tested in vitro on a tapeworm Raillietina tetragona Molin (Davaineidae) to evaluate its potential anthelmintic efficacy and ultrastructural changes i...
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doi:10.3109/13880209.2013.784920
更新日期:2013-10-01 00:00:00
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更新日期:2014-02-01 00:00:00
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更新日期:2013-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1076/phbi.39.s1.41.0007
更新日期:2001-01-01 00:00:00
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journal_title:Pharmaceutical biology
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doi:10.3109/13880209.2014.892513
更新日期:2014-11-01 00:00:00
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更新日期:2010-06-01 00:00:00
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更新日期:2013-08-01 00:00:00
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doi:10.3109/13880209.2015.1100638
更新日期:2016-01-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2016-01-01 00:00:00
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更新日期:2020-12-01 00:00:00
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更新日期:2012-09-01 00:00:00
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doi:10.3109/13880209.2011.574707
更新日期:2011-11-01 00:00:00
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更新日期:2005-01-01 00:00:00
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更新日期:2015-05-01 00:00:00
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更新日期:2016-01-01 00:00:00
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journal_title:Pharmaceutical biology
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doi:10.3109/13880209.2011.633920
更新日期:2012-07-01 00:00:00
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更新日期:2016-09-01 00:00:00
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更新日期:2016-01-01 00:00:00