In vitro and in vivo inhibition of mTOR by 1,25-dihydroxyvitamin D3 to improve early diabetic nephropathy via the DDIT4/TSC2/mTOR pathway.


:We investigated whether 1,25-dihydroxy-vitamin D3 (1,25(OH)2D3) could improve early diabetic nephropathy through the DNA-damage-inducible transcript 4/tuberous sclerosis 2/mammalian target of rapamycin pathway. Rat mesangial cells were cultured in media containing normal glucose or high glucose and were treated with or without 1,25(OH)2D3. Mesangial cells proliferation was measured. Streptozotocin-induced diabetic rats were injected intravenously with a recombinant lentivirus against the rat vitamin D receptor gene. Urinary and serum albumin, fasting plasma glucose, serum triglyceride, total cholesterol, calcium, parathyroid hormone and serum 25-dihydroxy-vitamin D (25(OH)D) levels, mean glomerular volume, glomerular basement membrane thickness and total kidney volume were determined. The expressions of vitamin D receptor, DNA-damage-inducible transcript 4, and mammalian target of rapamycin were measured. 1,25(OH)2D3 inhibited the proliferation of mesangial cells induced by hyperglycemia. 1,25(OH)2D3 also significantly reduced albumin excretion, mean glomerular volume, glomerular basement membrane, and total kidney volume in rats with diabetic nephropathy. The expression of DNA-damage-inducible transcript 4 was elevated by 1,25(OH)2D3 treatment. The phosphorylation of mammalian target of rapamycin was reduced by 1,25(OH)2D3 treatment. Vitamin D receptor gene silencing blocked all of the above results. The current study demonstrates that 1,25(OH)2D3 can effectively inhibit mesangial cells proliferation induced by hyperglycemia, thus suppressing the development of diabetic nephropathy. This study also shows that the nephron-protective effect of 1,25(OH)2D3 occurs partly through the DDIT4/TSC2/mTOR pathway.






Wang H,Wang J,Qu H,Wei H,Ji B,Yang Z,Wu J,He Q,Luo Y,Liu D,Duan Y,Liu F,Deng H




Has Abstract


2016-11-01 00:00:00














  • Clinical testing results and high patient satisfaction with a new needle-free device for growth hormone in young children.

    abstract::Fifty children ages 4-10 yr with type 1 diabetes mellitus volunteered to participate in a study to evaluate and compare a new needle-free device developed for growth hormone delivery. Children answered descriptive questions related to nervousness and worry, hurt or pain, redness or bleeding, and stinging and wetness. ...


    pub_type: 杂志文章


    authors: Silverstein JH,Murray FT,Malasanos T,Myers S,Johnson SB,Frye K,Grossman M

    更新日期:2001-06-01 00:00:00

  • An oral high dose of cholecalciferol restores vitamin D status in deficient postmenopausal HIV-1-infected women independently of protease inhibitors therapy: a pilot study.

    abstract::The best repletion and maintenance dosing regimens with cholecalciferol in vitamin D-deficient HIV-1 patients remain unknown. Protease inhibitors (PIs) have been shown to inhibit vitamin D 1α- and 25α-hydroxylation in hepatocyte and monocyte cultures. We therefore evaluated the effect of a single high dose of cholecal...


    pub_type: 杂志文章


    authors: Pepe J,Mezzaroma I,Fantauzzi A,Falciano M,Salotti A,Di Traglia M,Diacinti D,Biondi P,Cipriani C,Cilli M,Minisola S

    更新日期:2016-07-01 00:00:00

  • Complete evaluation of pituitary tumours in a single tertiary care institution.

    abstract:INTRODUCTION:We retrospectively evaluated all patients with pituitary tumours treated in our department from 1/1/1997 to 01/11/2014. PATIENTS AND METHODS:Two hundred and fifteen patients (124 females, 91 males, mean age 50.9 years) were treated because of pituitary tumours. All patients underwent basal hormonal analys...


    pub_type: 杂志文章


    authors: Askitis D,Tsitlakidis D,Müller N,Waschke A,Wolf G,Müller UA,Kloos C

    更新日期:2018-05-01 00:00:00

  • Metabolic syndrome and cancer: holistic or reductionist?

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    pub_type: 杂志文章,评审


    authors: Esposito K,Capuano A,Giugliano D

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  • Nicotinamide phosphoribosyltransferase leukocyte overexpression in Graves' opthalmopathy.

    abstract::To investigate the role of NAMPT/visfatin in euthyroid patients with Graves' disease without (GD) and with Graves' ophthalmopathy (GO), we analyzed NAMPT leukocyte expression and its serum concentration. This was a single-center, cross-sectional study with consecutive enrollment. In total, 149 patients diagnosed with ...


    pub_type: 杂志文章


    authors: Sawicka-Gutaj N,Budny B,Zybek-Kocik A,Sowiński J,Ziemnicka K,Waligórska-Stachura J,Ruchała M

    更新日期:2016-08-01 00:00:00

  • Synergistic insulinotropic action of succinate, acetate, and glucose esters in islets from normal and diabetic rats.

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    pub_type: 杂志文章


    authors: Sener A,Kadiata MM,Ladrière L,Malaisse WJ

    更新日期:1997-10-01 00:00:00

  • Insights on the phenotypic heterogenity of 11β-hydroxylase deficiency: clinical and genetic studies in two novel families.

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    pub_type: 杂志文章,评审


    authors: Valadares LP,Pfeilsticker ACV,de Brito Sousa SM,Cardoso SC,de Moraes OL,Gonçalves de Castro LC,de Oliveira RS,Lofrano-Porto A

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  • Dual-energy X-ray absorptiometry pitfalls in Thalassemia Major.

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    pub_type: 杂志文章,评审


    authors: Pellegrino F,Zatelli MC,Bondanelli M,Carnevale A,Cittanti C,Fortini M,Gamberini MR,Giganti M,Ambrosio MR

    更新日期:2019-09-01 00:00:00

  • The Langerhans islet cells of female rabbits are differentially affected by hypothyroidism depending on the islet size.

    abstract::Effects of hypothyroidism on the glucose and insulin levels are controversial, and its impact on the Langerhans islet morphology of adult subjects has been poorly addressed. In spite of hypothyroidism and diabetes mellitus are more frequent in females than in males, most studies using animal models have been done in m...


    pub_type: 杂志文章


    authors: Rodríguez-Castelán J,Nicolás L,Morimoto S,Cuevas E

    更新日期:2015-04-01 00:00:00

  • Identification of human somatostatin receptor 2 domains involved in internalization and signaling in QGP-1 pancreatic neuroendocrine tumor cell line.

    abstract::Somatostatin exerts inhibitory effects on hormone secretion and cell proliferation via five receptor subtypes (SST1-SST5), whose internalization is regulated by β-arrestins. The receptor domains involved in these effects have been only partially elucidated. The aim of the study is to characterize the molecular mechani...


    pub_type: 杂志文章


    authors: Cambiaghi V,Vitali E,Morone D,Peverelli E,Spada A,Mantovani G,Lania AG

    更新日期:2017-04-01 00:00:00

  • Diagnostic performance of Gallium-68 somatostatin receptor PET and PET/CT in patients with thoracic and gastroenteropancreatic neuroendocrine tumours: a meta-analysis.

    abstract:UNLABELLED:Gallium-68 somatostatin receptor (SMSR) positron emission tomography (PET) and positron emission tomography/computed tomography (PET/CT) are valuable diagnostic tools for patients with neuroendocrine tumours (NETs). To date, a meta-analysis about the diagnostic accuracy of these imaging methods is lacking. A...


    pub_type: 杂志文章,meta分析,评审


    authors: Treglia G,Castaldi P,Rindi G,Giordano A,Rufini V

    更新日期:2012-08-01 00:00:00

  • Effect of a monthly dose of calcidiol in improving vitamin D deficiency and secondary hyperparathyroidism in HIV-infected patients.

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    pub_type: 杂志文章


    authors: Bañón S,Rosillo M,Gómez A,Pérez-Elias MJ,Moreno S,Casado JL

    更新日期:2015-06-01 00:00:00

  • Evaluation of oxidative status in patients with hyperthyroidism.

    abstract::Data on the antioxidant levels enzyme in patients with hyperthyroidism are limited and conflicting. Therefore, the objective of this study was to evaluate the oxidative status using an automated method in patients with hyperthyroidism. Thirty-six subjects with hyperthyroidism and 30 healthy controls were enrolled in t...


    pub_type: 杂志文章


    authors: Aslan M,Cosar N,Celik H,Aksoy N,Dulger AC,Begenik H,Soyoral YU,Kucukoglu ME,Selek S

    更新日期:2011-10-01 00:00:00

  • Comparison of the therapeutic effects of prednisolone and nonsteroidal anti-inflammatory drugs in patients with subacute thyroiditis.

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    pub_type: 杂志文章


    authors: Sato J,Uchida T,Komiya K,Goto H,Takeno K,Suzuki R,Honda A,Himuro M,Watada H

    更新日期:2017-01-01 00:00:00

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    authors: Sotelo-Rivera I,Cote-Vélez A,Uribe RM,Charli JL,Joseph-Bravo P

    更新日期:2017-03-01 00:00:00

  • Alterations in hypothalamic-pituitary-adrenal function immediately after resection of adrenal adenomas in patients with Cushing's syndrome and others with incidentalomas and subclinical hypercortisolism.

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    pub_type: 杂志文章


    authors: Khawandanah D,ElAsmar N,Arafah BM

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    pub_type: 杂志文章


    authors: Topaloglu O,Gokay F,Kucukler K,Burnik FS,Mete T,Yavuz HC,Berker D,Guler S

    更新日期:2013-08-01 00:00:00

  • Dopamine D2 receptor stimulation alters G-protein expression in rat pituitary intermediate lobe melanotropes.

    abstract::Stimulation of dopamine D2 receptors inhibits melanotrope pro-opiomelanocortin (POMC) biosynthesis and alpha-melanocyte-stimulating hormone (MSH) secretion. These effects are mediated by G-protein alpha i- and alpha o-subunits and are reversed by stimulating receptors linked to activation of G alpha s protein. Melanot...


    pub_type: 杂志文章


    authors: Sands SA,Dickerson DS,Morris SJ,Chronwall BM

    更新日期:1997-06-01 00:00:00

  • Expression and characterization of recombinant beta-subunit hCG homodimer.

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    pub_type: 杂志文章


    authors: Lobel L,Pollak S,Wang S,Chaney M,Lustbader JW

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  • Endothelin-1 receptor blockade prevented the electrophysiological dysfunction in cardiac myocytes of streptozotocin-induced diabetic rats.

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    pub_type: 杂志文章


    authors: Ding Y,Zou R,Judd RL,Zhong J

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  • Thioredoxin-interacting protein: a critical link between autophagy disorders and pancreatic β-cell dysfunction.

    abstract::Thioredoxin-interacting protein (TXNIP) is a known important regulatory protein of islet β-cell biology and function, but the detailed mechanism is not clear. Autophagy plays a pivotal role in maintaining cellular homoeostasis. This study aimed to elucidate the influence of TXNIP on the autophagy of β-cell. In this st...


    pub_type: 杂志文章


    authors: Deng W,Li Y,Ren Z,He Q,Jia Y,Liu Y,Zhang W,Gan X,Liu D

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    pub_type: 杂志文章


    authors: Ferrara R,Ientile V,Arcoraci V,Ferrajolo C,Piccinni C,Fontana A,Benvenga S,Trifirò G

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  • Metabolic syndrome and the risk of cardiovascular complications in young patients with different phenotypes of polycystic ovary syndrome.

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    pub_type: 杂志文章


    authors: Krentowska A,Łebkowska A,Jacewicz-Święcka M,Hryniewicka J,Leśniewska M,Adamska A,Kowalska I

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  • Correction to: Anti-Müllerian hormone serum levels in systemic lupus erythematosus patients: influence of the disease severity and therapy on the ovarian reserve.

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    pub_type: 已发布勘误


    authors: Di Mario C,Petricca L,Gigante MR,Barini A,Barini A,Varriano V,Paglionico A,Cattani P,Ferraccioli G,Tolusso B,Gremese E

    更新日期:2019-02-01 00:00:00

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    pub_type: 杂志文章,评审


    authors: Lotinun S,Sibonga JD,Turner RT

    更新日期:2002-02-01 00:00:00

  • Insulin resistance and metabolic syndrome in normal-weight individuals.

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    pub_type: 杂志文章


    authors: Chen S,Chen Y,Liu X,Li M,Wu B,Li Y,Liang Y,Shao X,Holthöfer H,Zou H

    更新日期:2014-08-01 00:00:00

  • Association between leisure time physical activity and metabolic syndrome: a meta-analysis of prospective cohort studies.

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    pub_type: 杂志文章,meta分析


    authors: He D,Xi B,Xue J,Huai P,Zhang M,Li J

    更新日期:2014-06-01 00:00:00

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    abstract:PURPOSE:A large number of studies has investigated proopiomelanocortin processing in anterior pituitary corticotropes but little is known on proopiomelanocortin/ACTH degradation within these cells. The ubiquitin-proteasome system is an intracellular protein degradation pathway which has garnered considerable interest i...


    pub_type: 杂志文章


    authors: Sesta A,Cassarino MF,Cavagnini F,Pecori Giraldi F

    更新日期:2018-09-01 00:00:00

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    pub_type: 杂志文章


    authors: Langlois F,Manea A,Lim DST,McCartney S,Yedinak CG,Cetas JS,Fleseriu M

    更新日期:2019-03-01 00:00:00

  • Low 25 (OH) vitamin D levels are associated with autoimmune thyroid disease in polycystic ovary syndrome.

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    pub_type: 杂志文章


    authors: Muscogiuri G,Palomba S,Caggiano M,Tafuri D,Colao A,Orio F

    更新日期:2016-08-01 00:00:00