Abstract：
:(-)-Epigallocatechin-3‑gallate (EGCG), the predominant constituent of green tea, has been demonstrated to be neuroprotective against acute ischemic stroke. However, the long-term actions of EGCG on neurogenesis and functional recovery after ischemic stroke have not been identified. In this study, C57BL/6 mice underwent middle cerebral artery occlusion (60 min) followed by reperfusion for 28 days. Neural progenitor cells (NPCs) were isolated from ipsilateral subventricular zone (SVZ) at 14 days post-ischemia (dpi). The effects of EGCG on the proliferation and differentiation of NPCs were examined in vivo and in vitro. Behavioral assessments were made 3 days before MCAO and at 28 dpi. SVZ NPCs were stimulated with lipopolysaccharide (LPS) in vitro to mimic the inflammatory response after ischemic stroke. We found that 14 days treatment with EGCG significantly increased the proliferation of SVZ NPCs and the migration of SVZ neuroblasts, as well as functional recovery, perhaps through M2 phenotype induction in microglia. LPS stimulation promoted the neuronal differentiation in cultured NPCs from the ischemic SVZ. EGCG treatment (20 or 40 μM) further significantly increased the neuronal differentiation of LPS-stimulated SVZ NPCs. After screening for multiple signaling pathways, the AKT signaling pathway was found to be involved in EGCG-mediated proliferation and neuronal differentiation of NPCs in vitro. Taken together, our results reveal a previously uncharacterized role of EGCG in the augment of proliferation and neuronal differentiation of SVZ NPCs and subsequent spontaneous recovery after ischemic stroke. Thus, the beneficial effects of EGCG on neurogenesis and stroke recovery should be considered in developing therapeutic approaches.
journal_name
Mol Neurobioljournal_title
Molecular neurobiologyauthors
Zhang JC,Xu H,Yuan Y,Chen JY,Zhang YJ,Lin Y,Yuan SYdoi
10.1007/s12035-016-9924-0subject
Has Abstractpub_date
2017-07-01 00:00:00pages
3652-3664issue
5eissn
0893-7648issn
1559-1182pii
10.1007/s12035-016-9924-0journal_volume
54pub_type
杂志文章abstract：:PCDH19-Clustering Epilepsy (PCDH19-CE) is an infantile onset disorder caused by mutation of the X-linked PCDH19 gene. Intriguingly, heterozygous females are affected while hemizygous males are not. While there is compelling evidence that this disorder stems from the coexistence of WT and PCDH19-null cells, the cellula...
journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-020-02242-4
更新日期：2021-01-07 00:00:00
abstract：:Statins have proven their effectiveness in the treatment of cardiovascular disease. This class of drugs has also attracted attention as a potential treatment for dissimilar diseases such as certain types of cancers and neurodegenerative diseases. What appears to be a contradiction is that, in the case of cancer, it ha...
journal_title：Molecular neurobiology
pub_type： 杂志文章,评审
doi：10.1007/s12035-013-8496-5
更新日期：2013-10-01 00:00:00
abstract：:Axonal damage is a major factor contributing to disease progression in multiple sclerosis (MS) patients. On the histological level, acute axonal injury is most frequently analyzed by anti-amyloid precursor protein immunohistochemistry. To what extent this method truly detects axonal injury, and whether other proteins ...
journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-018-1353-9
更新日期：2019-06-01 00:00:00
abstract：:Stromal cell-derived factor-1 alpha (SDF-1a) or CXCL12 is an important cytokine with multiple functions in the brain during development and in adulthood. The inflammatory response initiated by spinal cord injury (SCI) involves the processing of interleukin-1beta (IL-1ß) and IL-18 mediated by caspase-1 which is under t...
journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-015-9203-5
更新日期：2016-07-01 00:00:00
abstract：:Experimental autoimmune encephalomyelitis (EAE) is the most used animal model of multiple sclerosis (MS) for the development of new therapies. Dopamine receptors can modulate EAE and MS development, thus highlighting the potential use of dopaminergic agonists in the treatment of MS, which has been poorly explored. Her...
journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-016-9717-5
更新日期：2017-03-01 00:00:00
abstract：:B-50 (GAP-43) is an axonal, plasma membrane-associated protein involved in growth cone morphology and function. We have conducted immunocytochemical, electron microscopic, and time-lapse experiments to visualize morphological consequences of local accumulations of B-50 at the plasma membrane of B-50-transfected PC-B2 ...
journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/BF02741362
更新日期：1999-08-01 00:00:00
abstract：:Depletion of oxygen and glucose even for brief periods is sufficient to cause cerebral ischemia, which is a predominant worldwide cause of motor deficits with the reduction of life quality and subsequently death. Hence, more insights regarding protective measures against ischemic events are becoming a major research g...
journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-016-0240-5
更新日期：2017-11-01 00:00:00
abstract：:Recently, we reported a correlation of scopolamine mediated decline in memory consolidation with increase in the expression of DNA methyltransferase 1 (DNMT1) and histone deacetylase 2 (HDAC2) in the mouse hippocampus. Memory consolidation is a protein synthesis-dependent process which involves the expression of synap...
journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-016-0047-4
更新日期：2017-09-01 00:00:00
abstract：:Parkinson's disease (PD) is a progressive neurodegenerative disease affecting over five million individuals worldwide. The exact molecular events underlying PD pathogenesis are still not clearly known. Glia maturation factor (GMF), a neuroinflammatory protein in the brain plays an important role in the pathogenesis of...
journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-018-0882-6
更新日期：2018-09-01 00:00:00
abstract：:A random-sequence peptide microarray can interrogate serum antibodies in a broad, unbiased fashion to generate disease-specific immunosignatures. This approach has been applied to cancer detection, diagnosis of infections, and interrogation of vaccine response. We hypothesized that there is an immunosignature specific...
journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-018-1354-8
更新日期：2019-06-01 00:00:00
abstract：:Voltage-dependent Ca2+ channels are important in the regulation of neuronal structure and function, and as a result, they have received considerable attention. Recent studies have begun to characterize the diversity of their properties and the relationship of this diversity to their various cellular functions. In part...
journal_title：Molecular neurobiology
pub_type： 杂志文章,评审
doi：10.1007/BF02740620
更新日期：1997-02-01 00:00:00
abstract：:Matrix metalloproteinase-9 (MMP-9) plays a crucial role in pathological processes of brain inflammation, injury, and neurodegeneration. Thrombin has been known as a regulator of MMP-9 expression and cells migration. However, the mechanisms underlying thrombin-induced MMP-9 expression in rat brain astrocytes (RBA-1 cel...
journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-013-8450-6
更新日期：2013-12-01 00:00:00
abstract：:Polyglutamine (polyQ) disorders share many similarities, such as a common mutation type in unrelated human causative genes, neurological character, and certain aspects of pathogenesis, including morphological and physiological neuronal alterations. The similarities in pathogenesis have been confirmed by findings that ...
journal_title：Molecular neurobiology
pub_type： 杂志文章,评审
doi：10.1007/s12035-012-8315-4
更新日期：2012-10-01 00:00:00
abstract：:Alzheimer's disease (AD) is the most common progressive neurodegenerative disorder. A defining hallmark of the AD brain is the presence of intraneuronal neurofibrillary tangles (NFTs) which are made up of abnormally modified tau, with aberrant phosphorylation being the most studied posttranslational modification (PTM)...
journal_title：Molecular neurobiology
pub_type： 杂志文章,评审
doi：10.1007/s12035-020-02084-0
更新日期：2020-12-01 00:00:00
abstract：:Cannabidiol (CBD), one of the most abundant Cannabis sativa-derived compounds, has been implicated with neuroprotective effect in several human pathologies. Until now, no undesired side effects have been associated with CBD. In this study, we evaluated CBD's neuroprotective effect in terminal differentiation (mature) ...
journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-014-8843-1
更新日期：2015-08-01 00:00:00
abstract：:Cultured hippocampal neurons represent the most widely used experimental substrate for in vitro electrophysiological studies. Nevertheless, in most cases, the nature of neuron under study is not identified as excitatory or inhibitory, or even worse, recorded neurons are considered as excitatory because of the paucity ...
journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-019-1506-5
更新日期：2019-09-01 00:00:00
abstract：:Stroke is the leading cause of severe disability, and lacunar stroke is related to cognitive decline and hemiparesis. There is no effective treatment for the majority of patients with stroke. Thus, stem cell-based regenerative medicine has drawn a growing body of attention due to the capabilities for trophic factor ex...
journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-015-9530-6
更新日期：2016-11-01 00:00:00
abstract：:TDP-43 accumulates in nerve cells of nearly all cases of amyotrophic lateral sclerosis (ALS; the commonest form of motor neuron disease) and in the majority of Tau-negative frontotemporal lobar degeneration (FTLD). There is currently no biochemical test or marker of disease activity for ALS or FTLD, and the clinical d...
journal_title：Molecular neurobiology
pub_type： 杂志文章,评审
doi：10.1007/s12035-018-0947-6
更新日期：2018-10-01 00:00:00
abstract：:People suffering from Huntington's disease (HD) present cognitive deficits. Hippocampal dysfunction has been involved in the HD learning and memory impairment, but proteins leading this dysregulation are not fully characterized. Here, we studied the contribution of the family of transcription factors myocyte enhancer ...
journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-020-02041-x
更新日期：2020-11-01 00:00:00
abstract：:Accumulating evidences indicate that endogenous modulators of excitatory synapses in the mammalian brain are potential targets for treating neuropsychiatric disorders. Indeed, glutamatergic and adenosinergic neurotransmissions were recently highlighted as potential targets for developing innovative anxiolytic drugs. A...
journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-015-9660-x
更新日期：2017-01-01 00:00:00
abstract：:The human natural killer-1 (HNK-1), 3-sulfonated glucuronic acid, is a glycoepitope marker of cell adhesion that participates in cell-cell and cell-extracellular matrix interactions and in neurite growth. Very little is known about the regulation of the HNK-1 glycan in neurodegenerative disease, particularly in Alzhei...
journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-015-9644-x
更新日期：2017-01-01 00:00:00
abstract：:Heterogenous nuclear ribonucleoproteins (hnRNPs) are a complex and functionally diverse family of RNA binding proteins with multifarious roles. They are involved, directly or indirectly, in alternative splicing, transcriptional and translational regulation, stress granule formation, cell cycle regulation, and axonal t...
journal_title：Molecular neurobiology
pub_type： 杂志文章,评审
doi：10.1007/s12035-020-02137-4
更新日期：2021-02-01 00:00:00
abstract：:Prion pathologies are characterized by the conformational conversion of the cellular prion protein (PrPC) into a pathological infectious isoform, known as PrPSc. The latter acquires different abnormal conformations, which are associated with specific pathological phenotypes. Recent evidence suggests that prions adapt ...
journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-018-1279-2
更新日期：2019-04-01 00:00:00
abstract：:Activation of RhoA/Rho kinase leads to growth cone collapse and neurite retraction. Although RhoA/Rho kinase inhibition has been shown to improve axon regeneration, remyelination and functional recovery, its role in neuronal cell death remains unclear. To determine whether RhoA/Rho kinase played a role in neuronal dea...
journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-016-0187-6
更新日期：2017-11-01 00:00:00
abstract：:Zika virus (ZIKV) infection is associated with abnormal functions of neuronal cells causing neurological disorders such as microcephaly in the newborns and Guillain-Barré syndrome in the adults. Typically, healthy brain growth is associated with normal neural stem cell proliferation, differentiation, and maturation. T...
journal_title：Molecular neurobiology
pub_type： 杂志文章,评审
doi：10.1007/s12035-018-1263-x
更新日期：2019-04-01 00:00:00
abstract：:The administration of the anorexigenic drug d,l-fenfluramine (Ponderax) to laboratory animals results in a dose-dependent reduction in presynaptically located serotonergic reuptake transporter protein. This long-term effect may represent an altered mechanism of synthesis of the transporter (downregulation). Alternativ...
journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/BF02740693
更新日期：1995-08-01 00:00:00
abstract：:The Shadoo protein (Sho) exhibits homology to the hydrophobic region of the cellular isoform of prion protein (PrPC). As prion-infected brains gradually accumulate infectivity-associated isoforms of prion protein (PrPSc), levels of mature endogenous Sho become reduced. To study the regulatory effect of the proteostati...
journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-019-1623-1
更新日期：2019-11-01 00:00:00
abstract：:Antiretroviral therapy (ART) restricts human immunodeficiency virus type one (HIV-1) replication and by so doing, improves the quality and longevity of life for infected people. Nonetheless, treatment can also lead to adverse clinical outcomes such as drug resistance and systemic adverse events. Both could be affected...
journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-018-1273-8
更新日期：2019-04-01 00:00:00
abstract：:The glutamate N-methyl-D-aspartate receptor (NMDAR) plays an essential role in the excitotoxic neural damage that follows ischaemic stroke. Because the sigma-1 receptor (σ1R) can regulate NMDAR transmission, exogenous and putative endogenous regulators of σ1R have been investigated using animal models of ischaemic str...
journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-017-0697-x
更新日期：2018-06-01 00:00:00
abstract：:Epigallocatechin-3-gallate (EGCG), a catechin found in green tea, has been previously investigated for its neuroprotective effects in vitro and in vivo. In the present study, we aimed to evaluate its possible beneficial effects in a well-established preclinical mixed model of familial Alzheimer's disease (AD) and type...
journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-019-01849-6
更新日期：2020-04-01 00:00:00
